Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P36149 (BET3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking protein particle complex subunit BET3

Short name=TRAPP subunit BET3
Alternative name(s):
Transport protein particle 22 kDa subunit
Gene names
Name:BET3
Ordered Locus Names:YKR068C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication. Ref.4 Ref.5 Ref.6 Ref.9 Ref.10

Subunit structure

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.5 Ref.6 Ref.9 Ref.10

Subcellular location

Golgi apparatuscis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.6 Ref.10.

Miscellaneous

Present with 2370 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRAPP small subunits family. BET3 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Trafficking protein particle complex subunit BET3
PRO_0000211578

Amino acid modifications

Lipidation801S-palmitoyl cysteine Ref.8

Experimental info

Mutagenesis801C → S: Loss of palmitoylation. Ref.8

Secondary structure

.............................. 193
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36149 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: C802D8A3563DE82B

FASTA19322,129
        10         20         30         40         50         60 
MVSTTQSRSL KAMGEEIWKN KTEKINTELF TLTYGSIVAQ LCQDYERDFN KVNDHLYSMG 

        70         80         90        100        110        120 
YNIGCRLIED FLARTALPRC ENLVKTSEVL SKCAFKIFLN ITPNITNWSH NKDTFSLILD 

       130        140        150        160        170        180 
ENPLADFVEL PMDAMKSLWY SNILCGVLKG SLEMVQLDCD VWFVSDILRG DSQTEIKVKL 

       190 
NRILKDEIPI GED 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs."
Rossi G., Kolstad K., Stone S., Palluault F., Ferro-Novick S.
Mol. Biol. Cell 6:1769-1780(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX, CHARACTERIZATION.
[6]"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization."
Turnbull A.P., Kummel D., Prinz B., Holz C., Schultchen J., Lang C., Niesen F.H., Hofmann K.P., Delbruck H., Behlke J., Muller E.C., Jarosch E., Sommer T., Heinemann U.
EMBO J. 24:875-884(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYS-80, PALMITOYLATION AT CYS-80.
[9]"Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
Yip C.K., Berscheminski J., Walz T.
Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[10]"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy."
Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J.
Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28293 Genomic DNA. Translation: CAA82147.1.
AY557896 Genomic DNA. Translation: AAS56222.1.
BK006944 Genomic DNA. Translation: DAA09219.1.
PIRS38144.
RefSeqNP_012994.1. NM_001179858.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70D/E/J/K/P/Q/V/W1-193[»]
ProteinModelPortalP36149.
SMRP36149. Positions 6-193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34199. 77 interactions.
DIPDIP-1710N.
IntActP36149. 27 interactions.
MINTMINT-401400.
STRING4932.YKR068C.

Proteomic databases

PaxDbP36149.
PeptideAtlasP36149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR068C; YKR068C; YKR068C.
GeneID853942.
KEGGsce:YKR068C.

Organism-specific databases

CYGDYKR068c.
SGDS000001776. BET3.

Phylogenomic databases

eggNOGNOG286899.
GeneTreeENSGT00390000003880.
HOGENOMHOG000186184.
OMAVGFKMFL.
OrthoDBEOG7X9GK1.

Enzyme and pathway databases

BioCycYEAST:G3O-32034-MONOMER.

Gene expression databases

GenevestigatorP36149.

Family and domain databases

InterProIPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
IPR016721. TRAPP_I_complex_Bet3.
[Graphical view]
PANTHERPTHR13048. PTHR13048. 1 hit.
PfamPF04051. TRAPP. 1 hit.
[Graphical view]
PIRSFPIRSF018293. TRAPP_I_complex_Bet3. 1 hit.
SUPFAMSSF111126. SSF111126. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP36149.
NextBio975333.
PROP36149.

Entry information

Entry nameBET3_YEAST
AccessionPrimary (citable) accession number: P36149
Secondary accession number(s): D6VXC9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references