Trafficking protein particle complex subunit BET3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36149 (BET3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Trafficking protein particle complex subunit BET3
Short name=TRAPP subunit BET3

Alternative name(s):
Transport protein particle 22 kDa subunit

Gene names

Name:	BET3
Ordered Locus Names:	YKR068C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication. Ref.4 Ref.5 Ref.6 Ref.9 Ref.10
Subunit structure	Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.5 Ref.6 Ref.9 Ref.10
Subcellular location	Golgi apparatus › cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.6 Ref.10.
Miscellaneous	Present with 2370 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TRAPP small subunits family. BET3 subfamily.

Ontologies

Keywords
Biological process	Autophagy ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.6 Ref.4. Source: SGD autophagy Inferred from electronic annotation. Source: UniProtKB-KW intra-Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	TRAPP complex Inferred from direct assay PubMed 10727015 Ref.5. Source: SGD cis-Golgi network membrane Inferred from direct assay Ref.5. Source: SGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell pre-autophagosomal structure Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
BET5	Q03630	10	EBI-3567,EBI-3580
TRS20	P38334	11	EBI-3567,EBI-19468
TRS23	Q03784	10	EBI-3567,EBI-19474
TRS31	Q03337	13	EBI-3567,EBI-38770
TRS33	Q99394	8	EBI-3567,EBI-19480

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Trafficking protein particle complex subunit BET3

PRO_0000211578

Amino acid modifications

Lipidation

S-palmitoyl cysteine Ref.8

Experimental info

Mutagenesis

C → S: Loss of palmitoylation. Ref.8

Secondary structure

193

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P36149 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: C802D8A3563DE82B
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FASTA

193

22,129

        10         20         30         40         50         60 
MVSTTQSRSL KAMGEEIWKN KTEKINTELF TLTYGSIVAQ LCQDYERDFN KVNDHLYSMG 

        70         80         90        100        110        120 
YNIGCRLIED FLARTALPRC ENLVKTSEVL SKCAFKIFLN ITPNITNWSH NKDTFSLILD 

       130        140        150        160        170        180 
ENPLADFVEL PMDAMKSLWY SNILCGVLKG SLEMVQLDCD VWFVSDILRG DSQTEIKVKL 

       190 
NRILKDEIPI GED

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs." Rossi G., Kolstad K., Stone S., Palluault F., Ferro-Novick S. Mol. Biol. Cell 6:1769-1780(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion." Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S. EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX, CHARACTERIZATION.
[6]	"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport." Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S. Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization." Turnbull A.P., Kummel D., Prinz B., Holz C., Schultchen J., Lang C., Niesen F.H., Hofmann K.P., Delbruck H., Behlke J., Muller E.C., Jarosch E., Sommer T., Heinemann U. EMBO J. 24:875-884(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-80, PALMITOYLATION AT CYS-80.
[9]	"Molecular architecture of the TRAPPII complex and implications for vesicle tethering." Yip C.K., Berscheminski J., Walz T. Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[10]	"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy." Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J. Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z28293 Genomic DNA. Translation: CAA82147.1.
AY557896 Genomic DNA. Translation: AAS56222.1.
BK006944 Genomic DNA. Translation: DAA09219.1.

PIR

S38144.

RefSeq

NP_012994.1. NM_001179858.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CUE	X-ray	3.70	D/E/J/K/P/Q/V/W	1-193	[»]

ProteinModelPortal

P36149.

SMR

P36149. Positions 6-193.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1710N.

IntAct

P36149. 26 interactions.

MINT

MINT-401400.

STRING

4932.YKR068C.

Proteomic databases

PaxDb

P36149.

PeptideAtlas

P36149.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKR068C; YKR068C; YKR068C.

GeneID

853942.

KEGG

sce:YKR068C.

Organism-specific databases

CYGD

YKR068c.

SGD

S000001776. BET3.

Phylogenomic databases

eggNOG

NOG286899.

GeneTree

ENSGT00390000003880.

HOGENOM

HOG000186184.

OMA

WFVQDQL.

OrthoDB

EOG43V34Q.

Gene expression databases

Genevestigator

P36149.

GermOnline

YKR068C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
IPR016721. TRAPP_I_complex_Bet3.
[Graphical view]

PANTHER

PTHR13048. PTHR13048. 1 hit.

Pfam

PF04051. TRAPP. 1 hit.
[Graphical view]

PIRSF

PIRSF018293. TRAPP_I_complex_Bet3. 1 hit.

SUPFAM

SSF111126. Haem_NO-bd. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P36149.

NextBio

975333.

Entry information

Entry name

BET3_YEAST

Accession

Primary (citable) accession number: P36149
Secondary accession number(s): D6VXC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents