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P36149

- BET3_YEAST

UniProt

P36149 - BET3_YEAST

Protein

Trafficking protein particle complex subunit BET3

Gene

BET3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication.5 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. ER to Golgi vesicle-mediated transport Source: SGD
    3. intra-Golgi vesicle-mediated transport Source: SGD
    4. positive regulation of Rab GTPase activity Source: GOC

    Keywords - Biological processi

    Autophagy, ER-Golgi transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32034-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trafficking protein particle complex subunit BET3
    Short name:
    TRAPP subunit BET3
    Alternative name(s):
    Transport protein particle 22 kDa subunit
    Gene namesi
    Name:BET3
    Ordered Locus Names:YKR068C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR068c.
    SGDiS000001776. BET3.

    Subcellular locationi

    GO - Cellular componenti

    1. cis-Golgi network membrane Source: SGD
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. pre-autophagosomal structure Source: UniProtKB-SubCell
    4. TRAPPIII protein complex Source: SGD
    5. TRAPPII protein complex Source: SGD
    6. TRAPPI protein complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi80 – 801C → S: Loss of palmitoylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 193193Trafficking protein particle complex subunit BET3PRO_0000211578Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi80 – 801S-palmitoyl cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiP36149.
    PaxDbiP36149.
    PeptideAtlasiP36149.

    Expressioni

    Gene expression databases

    GenevestigatoriP36149.

    Interactioni

    Subunit structurei

    Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-3567,EBI-3567
    BET5Q0363010EBI-3567,EBI-3580
    TRS20P3833411EBI-3567,EBI-19468
    TRS23Q0378410EBI-3567,EBI-19474
    TRS31Q0333713EBI-3567,EBI-38770
    TRS33Q993948EBI-3567,EBI-19480

    Protein-protein interaction databases

    BioGridi34199. 77 interactions.
    DIPiDIP-1710N.
    IntActiP36149. 27 interactions.
    MINTiMINT-401400.
    STRINGi4932.YKR068C.

    Structurei

    Secondary structure

    1
    193
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 124
    Helixi16 – 183
    Beta strandi19 – 224
    Beta strandi24 – 263
    Helixi27 – 337
    Helixi37 – 448
    Turni45 – 473
    Helixi49 – 5810
    Helixi66 – 749
    Helixi83 – 9816
    Helixi123 – 1253
    Turni132 – 1365
    Turni140 – 1445
    Helixi153 – 1553
    Beta strandi160 – 1634
    Beta strandi176 – 1794

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3CUEX-ray3.70D/E/J/K/P/Q/V/W1-193[»]
    ProteinModelPortaliP36149.
    SMRiP36149. Positions 6-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP36149.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG286899.
    GeneTreeiENSGT00390000003880.
    HOGENOMiHOG000186184.
    OMAiLWYSNIL.
    OrthoDBiEOG7X9GK1.

    Family and domain databases

    InterProiIPR024096. NO_sig/Golgi_transp_ligand-bd.
    IPR007194. TRAPP_component.
    IPR016721. TRAPP_I_complex_Bet3.
    [Graphical view]
    PANTHERiPTHR13048. PTHR13048. 1 hit.
    PfamiPF04051. TRAPP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018293. TRAPP_I_complex_Bet3. 1 hit.
    SUPFAMiSSF111126. SSF111126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSTTQSRSL KAMGEEIWKN KTEKINTELF TLTYGSIVAQ LCQDYERDFN    50
    KVNDHLYSMG YNIGCRLIED FLARTALPRC ENLVKTSEVL SKCAFKIFLN 100
    ITPNITNWSH NKDTFSLILD ENPLADFVEL PMDAMKSLWY SNILCGVLKG 150
    SLEMVQLDCD VWFVSDILRG DSQTEIKVKL NRILKDEIPI GED 193
    Length:193
    Mass (Da):22,129
    Last modified:June 1, 1994 - v1
    Checksum:iC802D8A3563DE82B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28293 Genomic DNA. Translation: CAA82147.1.
    AY557896 Genomic DNA. Translation: AAS56222.1.
    BK006944 Genomic DNA. Translation: DAA09219.1.
    PIRiS38144.
    RefSeqiNP_012994.1. NM_001179858.1.

    Genome annotation databases

    EnsemblFungiiYKR068C; YKR068C; YKR068C.
    GeneIDi853942.
    KEGGisce:YKR068C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28293 Genomic DNA. Translation: CAA82147.1 .
    AY557896 Genomic DNA. Translation: AAS56222.1 .
    BK006944 Genomic DNA. Translation: DAA09219.1 .
    PIRi S38144.
    RefSeqi NP_012994.1. NM_001179858.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3CUE X-ray 3.70 D/E/J/K/P/Q/V/W 1-193 [» ]
    ProteinModelPortali P36149.
    SMRi P36149. Positions 6-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34199. 77 interactions.
    DIPi DIP-1710N.
    IntActi P36149. 27 interactions.
    MINTi MINT-401400.
    STRINGi 4932.YKR068C.

    Proteomic databases

    MaxQBi P36149.
    PaxDbi P36149.
    PeptideAtlasi P36149.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR068C ; YKR068C ; YKR068C .
    GeneIDi 853942.
    KEGGi sce:YKR068C.

    Organism-specific databases

    CYGDi YKR068c.
    SGDi S000001776. BET3.

    Phylogenomic databases

    eggNOGi NOG286899.
    GeneTreei ENSGT00390000003880.
    HOGENOMi HOG000186184.
    OMAi LWYSNIL.
    OrthoDBi EOG7X9GK1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32034-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P36149.
    NextBioi 975333.
    PROi P36149.

    Gene expression databases

    Genevestigatori P36149.

    Family and domain databases

    InterProi IPR024096. NO_sig/Golgi_transp_ligand-bd.
    IPR007194. TRAPP_component.
    IPR016721. TRAPP_I_complex_Bet3.
    [Graphical view ]
    PANTHERi PTHR13048. PTHR13048. 1 hit.
    Pfami PF04051. TRAPP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018293. TRAPP_I_complex_Bet3. 1 hit.
    SUPFAMi SSF111126. SSF111126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs."
      Rossi G., Kolstad K., Stone S., Palluault F., Ferro-Novick S.
      Mol. Biol. Cell 6:1769-1780(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
      Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
      EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX, CHARACTERIZATION.
    6. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
      Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
      Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization."
      Turnbull A.P., Kummel D., Prinz B., Holz C., Schultchen J., Lang C., Niesen F.H., Hofmann K.P., Delbruck H., Behlke J., Muller E.C., Jarosch E., Sommer T., Heinemann U.
      EMBO J. 24:875-884(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYS-80, PALMITOYLATION AT CYS-80.
    9. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
      Yip C.K., Berscheminski J., Walz T.
      Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
    10. Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBET3_YEAST
    AccessioniPrimary (citable) accession number: P36149
    Secondary accession number(s): D6VXC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2370 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3