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Protein

Trafficking protein particle complex subunit BET3

Gene

BET3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication.5 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • ER to Golgi vesicle-mediated transport Source: SGD
  • intra-Golgi vesicle-mediated transport Source: SGD
  • positive regulation of GTPase activity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32034-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex subunit BET3
Short name:
TRAPP subunit BET3
Alternative name(s):
Transport protein particle 22 kDa subunit
Gene namesi
Name:BET3
Ordered Locus Names:YKR068C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR068C.
SGDiS000001776. BET3.

Subcellular locationi

GO - Cellular componenti

  • cis-Golgi network membrane Source: SGD
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • pre-autophagosomal structure Source: UniProtKB-SubCell
  • TRAPPIII protein complex Source: SGD
  • TRAPPII protein complex Source: SGD
  • TRAPPI protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi80 – 801C → S: Loss of palmitoylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Trafficking protein particle complex subunit BET3PRO_0000211578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi80 – 801S-palmitoyl cysteine1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiP36149.

PTM databases

SwissPalmiP36149.

Interactioni

Subunit structurei

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-3567,EBI-3567
BET5Q0363010EBI-3567,EBI-3580
TRS20P3833411EBI-3567,EBI-19468
TRS23Q0378410EBI-3567,EBI-19474
TRS31Q0333713EBI-3567,EBI-38770
TRS33Q993948EBI-3567,EBI-19480

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi34199. 78 interactions.
DIPiDIP-1710N.
IntActiP36149. 27 interactions.
MINTiMINT-401400.

Structurei

Secondary structure

1
193
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 124
Helixi16 – 183
Beta strandi19 – 224
Beta strandi24 – 263
Helixi27 – 337
Helixi37 – 448
Turni45 – 473
Helixi49 – 5810
Helixi66 – 749
Helixi83 – 9816
Helixi123 – 1253
Turni132 – 1365
Turni140 – 1445
Helixi153 – 1553
Beta strandi160 – 1634
Beta strandi176 – 1794

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70D/E/J/K/P/Q/V/W1-193[»]
ProteinModelPortaliP36149.
SMRiP36149. Positions 6-193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36149.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000003880.
HOGENOMiHOG000186184.
InParanoidiP36149.
KOiK20302.
OMAiVEAHFIS.
OrthoDBiEOG092C4STP.

Family and domain databases

CDDicd14942. TRAPPC3_bet3. 1 hit.
InterProiIPR016721. Bet3.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
[Graphical view]
PANTHERiPTHR13048. PTHR13048. 1 hit.
PfamiPF04051. TRAPP. 1 hit.
[Graphical view]
PIRSFiPIRSF018293. TRAPP_I_complex_Bet3. 1 hit.
SUPFAMiSSF111126. SSF111126. 1 hit.

Sequencei

Sequence statusi: Complete.

P36149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSTTQSRSL KAMGEEIWKN KTEKINTELF TLTYGSIVAQ LCQDYERDFN
60 70 80 90 100
KVNDHLYSMG YNIGCRLIED FLARTALPRC ENLVKTSEVL SKCAFKIFLN
110 120 130 140 150
ITPNITNWSH NKDTFSLILD ENPLADFVEL PMDAMKSLWY SNILCGVLKG
160 170 180 190
SLEMVQLDCD VWFVSDILRG DSQTEIKVKL NRILKDEIPI GED
Length:193
Mass (Da):22,129
Last modified:June 1, 1994 - v1
Checksum:iC802D8A3563DE82B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28293 Genomic DNA. Translation: CAA82147.1.
AY557896 Genomic DNA. Translation: AAS56222.1.
BK006944 Genomic DNA. Translation: DAA09219.1.
PIRiS38144.
RefSeqiNP_012994.1. NM_001179858.1.

Genome annotation databases

EnsemblFungiiYKR068C; YKR068C; YKR068C.
GeneIDi853942.
KEGGisce:YKR068C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28293 Genomic DNA. Translation: CAA82147.1.
AY557896 Genomic DNA. Translation: AAS56222.1.
BK006944 Genomic DNA. Translation: DAA09219.1.
PIRiS38144.
RefSeqiNP_012994.1. NM_001179858.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70D/E/J/K/P/Q/V/W1-193[»]
ProteinModelPortaliP36149.
SMRiP36149. Positions 6-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34199. 78 interactions.
DIPiDIP-1710N.
IntActiP36149. 27 interactions.
MINTiMINT-401400.

PTM databases

SwissPalmiP36149.

Proteomic databases

MaxQBiP36149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR068C; YKR068C; YKR068C.
GeneIDi853942.
KEGGisce:YKR068C.

Organism-specific databases

EuPathDBiFungiDB:YKR068C.
SGDiS000001776. BET3.

Phylogenomic databases

GeneTreeiENSGT00390000003880.
HOGENOMiHOG000186184.
InParanoidiP36149.
KOiK20302.
OMAiVEAHFIS.
OrthoDBiEOG092C4STP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32034-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Miscellaneous databases

EvolutionaryTraceiP36149.
PROiP36149.

Family and domain databases

CDDicd14942. TRAPPC3_bet3. 1 hit.
InterProiIPR016721. Bet3.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
[Graphical view]
PANTHERiPTHR13048. PTHR13048. 1 hit.
PfamiPF04051. TRAPP. 1 hit.
[Graphical view]
PIRSFiPIRSF018293. TRAPP_I_complex_Bet3. 1 hit.
SUPFAMiSSF111126. SSF111126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBET3_YEAST
AccessioniPrimary (citable) accession number: P36149
Secondary accession number(s): D6VXC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2370 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.