Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol-3-phosphate O-acyltransferase 2

Gene

GPT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-3-P/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase.1 Publication

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate O-acyltransferase 2 (GPT2), Glycerol-3-phosphate O-acyltransferase 1 (SCT1)
  2. Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase (SLC1)
  3. Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, mitochondrial (TAM41)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • glycerol-3-phosphate O-acyltransferase activity Source: SGD
  • glycerone-phosphate O-acyltransferase activity Source: SGD

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • phospholipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-4095.
YEAST:MONOMER3O-4095.
BRENDAi2.3.1.15. 984.
UniPathwayiUPA00557; UER00612.

Chemistry

SwissLipidsiSLP:000000047.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate O-acyltransferase 2 (EC:2.3.1.15)
Short name:
G-3-P acyltransferase 2
Alternative name(s):
Dihydroxyacetone phosphate acyltransferase 2 (EC:2.3.1.42)
Short name:
DHAP-AT 2
Glycerol-3-phosphate / dihydroxyacetone phosphate acyltransferase 2
Gene namesi
Name:GPT2
Synonyms:GAT1
Ordered Locus Names:YKR067W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR067W.
SGDiS000001775. GPT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030CytoplasmicSequence analysisAdd
BLAST
Transmembranei31 – 5525HelicalSequence analysisAdd
BLAST
Topological domaini56 – 6813ExtracellularSequence analysisAdd
BLAST
Transmembranei69 – 8517HelicalSequence analysisAdd
BLAST
Topological domaini86 – 501416CytoplasmicSequence analysisAdd
BLAST
Transmembranei502 – 52423HelicalSequence analysisAdd
BLAST
Topological domaini525 – 53814ExtracellularSequence analysisAdd
BLAST
Transmembranei539 – 55517HelicalSequence analysisAdd
BLAST
Topological domaini556 – 743188CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 743743Glycerol-3-phosphate O-acyltransferase 2PRO_0000195258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei632 – 6321PhosphoserineCombined sources
Modified residuei637 – 6371PhosphoserineCombined sources
Modified residuei647 – 6471PhosphoserineCombined sources
Modified residuei651 – 6511PhosphoserineCombined sources
Modified residuei654 – 6541PhosphoserineCombined sources
Modified residuei657 – 6571PhosphoserineCombined sources
Modified residuei664 – 6641PhosphoserineCombined sources
Modified residuei668 – 6681PhosphoserineCombined sources
Modified residuei671 – 6711PhosphoserineCombined sources
Modified residuei673 – 6731PhosphothreonineCombined sources
Modified residuei688 – 6881PhosphoserineCombined sources
Modified residuei692 – 6921PhosphothreonineCombined sources
Modified residuei693 – 6931PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36148.

PTM databases

iPTMnetiP36148.

Interactioni

Protein-protein interaction databases

BioGridi34198. 101 interactions.
DIPiDIP-6620N.
IntActiP36148. 39 interactions.
MINTiMINT-643778.

Structurei

3D structure databases

ProteinModelPortaliP36148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000067105.
HOGENOMiHOG000191288.
InParanoidiP36148.
KOiK13507.
OMAiGLHYFHR.
OrthoDBiEOG092C0TE2.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAADHNA AKPIPHVPQA SRRYKNSYNG FVYNIHTWLY DVSVFLFNIL
60 70 80 90 100
FTIFFREIKV RGAYNVPEVG VPTILVCAPH ANQFIDPALV MSQTRLLKTS
110 120 130 140 150
AGKSRSRMPC FVTAESSFKK RFISFFGHAM GGIPVPRIQD NLKPVDENLE
160 170 180 190 200
IYAPDLKNHP EIIKGRSKNP QTTPVNFTKR FSAKSLLGLP DYLSNAQIKE
210 220 230 240 250
IPDDETIILS SPFRTSKSKV VELLTNGTNF KYAEKIDNTE TFQSVFDHLH
260 270 280 290 300
TKGCVGIFPE GGSHDRPSLL PIKAGVAIMA LGAVAADPTM KVAVVPCGLH
310 320 330 340 350
YFHRNKFRSR AVLEYGEPIV VDGKYGEMYK DSPRETVSKL LKKITNSLFS
360 370 380 390 400
VTENAPDYDT LMVIQAARRL YQPVKVRLPL PAIVEINRRL LFGYSKFKDD
410 420 430 440 450
PRIIHLKKLV YDYNRKLDSV GLKDHQVMQL KTTKLEALRC FVTLIVRLIK
460 470 480 490 500
FSVFAILSLP GSILFTPIFI ICRVYSEKKA KEGLKKSLVK IKGTDLLATW
510 520 530 540 550
KLIVALILAP ILYVTYSILL IILARKQHYC RIWVPSNNAF IQFVYFYALL
560 570 580 590 600
VFTTYSSLKT GEIGVDLFKS LRPLFVSIVY PGKKIEEIQT TRKNLSLELT
610 620 630 640 650
AVCNDLGPLV FPDYDKLATE IFSKRDGYDV SSDAESSISR MSVQSRSRSS
660 670 680 690 700
SIHSIGSLAS NALSRVNSRG SLTDIPIFSD AKQGQWKSEG ETSEDEDEFD
710 720 730 740
EKNPAIVQTA RSSDLNKENS RNTNISSKIA SLVRQKREHE KKE
Length:743
Mass (Da):83,645
Last modified:June 1, 1994 - v1
Checksum:i84B9946E56B82F15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311354 Genomic DNA. Translation: CAC85303.1.
Z28292 Genomic DNA. Translation: CAA82146.1.
BK006944 Genomic DNA. Translation: DAA09218.1.
PIRiS38143.
RefSeqiNP_012993.3. NM_001179857.3.

Genome annotation databases

EnsemblFungiiYKR067W; YKR067W; YKR067W.
GeneIDi853941.
KEGGisce:YKR067W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311354 Genomic DNA. Translation: CAC85303.1.
Z28292 Genomic DNA. Translation: CAA82146.1.
BK006944 Genomic DNA. Translation: DAA09218.1.
PIRiS38143.
RefSeqiNP_012993.3. NM_001179857.3.

3D structure databases

ProteinModelPortaliP36148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34198. 101 interactions.
DIPiDIP-6620N.
IntActiP36148. 39 interactions.
MINTiMINT-643778.

Chemistry

SwissLipidsiSLP:000000047.

PTM databases

iPTMnetiP36148.

Proteomic databases

MaxQBiP36148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR067W; YKR067W; YKR067W.
GeneIDi853941.
KEGGisce:YKR067W.

Organism-specific databases

EuPathDBiFungiDB:YKR067W.
SGDiS000001775. GPT2.

Phylogenomic databases

GeneTreeiENSGT00530000067105.
HOGENOMiHOG000191288.
InParanoidiP36148.
KOiK13507.
OMAiGLHYFHR.
OrthoDBiEOG092C0TE2.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciMetaCyc:MONOMER3O-4095.
YEAST:MONOMER3O-4095.
BRENDAi2.3.1.15. 984.

Miscellaneous databases

PROiP36148.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPT2_YEAST
AccessioniPrimary (citable) accession number: P36148
Secondary accession number(s): D6VXC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.