Glycerol-3-phosphate O-acyltransferase 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36148 (GPT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate O-acyltransferase 2
Short name=G-3-P acyltransferase 2
EC=2.3.1.15

Alternative name(s):
Dihydroxyacetone phosphate acyltransferase 2
Short name=DHAP-AT 2
EC=2.3.1.42
Glycerol-3-phosphate / dihydroxyacetone phosphate acyltransferase 2

Gene names

Name:	GPT2
Synonyms:	GAT1
Ordered Locus Names:	YKR067W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	G-3-P/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase. Ref.1
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Miscellaneous	Present with 3100 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from genetic interaction Ref.1. Source: SGD
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: SGD
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from genetic interaction Ref.1. Source: SGD glycerone-phosphate O-acyltransferase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 743

743

Glycerol-3-phosphate O-acyltransferase 2

PRO_0000195258

Regions

Topological domain

1 – 30

Cytoplasmic Potential

Transmembrane

31 – 55

Helical; Potential

Topological domain

56 – 68

Extracellular Potential

Transmembrane

69 – 85

Helical; Potential

Topological domain

86 – 501

416

Cytoplasmic Potential

Transmembrane

502 – 524

Helical; Potential

Topological domain

525 – 538

Extracellular Potential

Transmembrane

539 – 555

Helical; Potential

Topological domain

556 – 743

188

Cytoplasmic Potential

Amino acid modifications

Modified residue

211

Phosphoserine Ref.9

Modified residue

631

Phosphoserine Ref.9

Modified residue

632

Phosphoserine Ref.8 Ref.9

Modified residue

637

Phosphoserine Ref.8

Modified residue

654

Phosphoserine Ref.7

Modified residue

668

Phosphoserine Ref.5 Ref.7 Ref.9

Modified residue

671

Phosphoserine Ref.5 Ref.7 Ref.9

Modified residue

673

Phosphothreonine Ref.9

Modified residue

688

Phosphoserine Ref.8 Ref.9

Modified residue

692

Phosphothreonine Ref.9

Modified residue

693

Phosphoserine Ref.8 Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P36148 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 84B9946E56B82F15
Show »

FASTA

743

83,645

        10         20         30         40         50         60 
MSAPAADHNA AKPIPHVPQA SRRYKNSYNG FVYNIHTWLY DVSVFLFNIL FTIFFREIKV 

        70         80         90        100        110        120 
RGAYNVPEVG VPTILVCAPH ANQFIDPALV MSQTRLLKTS AGKSRSRMPC FVTAESSFKK 

       130        140        150        160        170        180 
RFISFFGHAM GGIPVPRIQD NLKPVDENLE IYAPDLKNHP EIIKGRSKNP QTTPVNFTKR 

       190        200        210        220        230        240 
FSAKSLLGLP DYLSNAQIKE IPDDETIILS SPFRTSKSKV VELLTNGTNF KYAEKIDNTE 

       250        260        270        280        290        300 
TFQSVFDHLH TKGCVGIFPE GGSHDRPSLL PIKAGVAIMA LGAVAADPTM KVAVVPCGLH 

       310        320        330        340        350        360 
YFHRNKFRSR AVLEYGEPIV VDGKYGEMYK DSPRETVSKL LKKITNSLFS VTENAPDYDT 

       370        380        390        400        410        420 
LMVIQAARRL YQPVKVRLPL PAIVEINRRL LFGYSKFKDD PRIIHLKKLV YDYNRKLDSV 

       430        440        450        460        470        480 
GLKDHQVMQL KTTKLEALRC FVTLIVRLIK FSVFAILSLP GSILFTPIFI ICRVYSEKKA 

       490        500        510        520        530        540 
KEGLKKSLVK IKGTDLLATW KLIVALILAP ILYVTYSILL IILARKQHYC RIWVPSNNAF 

       550        560        570        580        590        600 
IQFVYFYALL VFTTYSSLKT GEIGVDLFKS LRPLFVSIVY PGKKIEEIQT TRKNLSLELT 

       610        620        630        640        650        660 
AVCNDLGPLV FPDYDKLATE IFSKRDGYDV SSDAESSISR MSVQSRSRSS SIHSIGSLAS 

       670        680        690        700        710        720 
NALSRVNSRG SLTDIPIFSD AKQGQWKSEG ETSEDEDEFD EKNPAIVQTA RSSDLNKENS 

       730        740 
RNTNISSKIA SLVRQKREHE KKE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The initial step of the glycerolipid pathway: identification of glycerol-3-phosphate / dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae." Zheng Z., Zou J. J. Biol. Chem. 276:41710-41716(2001) [PubMed: 11544256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204660 / DBY746.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668 AND SER-671, MASS SPECTROMETRY. Strain: SUB592.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-654; SER-668 AND SER-671, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632; SER-637; SER-688 AND SER-693, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-211; SER-631; SER-632; SER-668; SER-671; THR-673; SER-688; THR-692 AND SER-693, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ311354 Genomic DNA. Translation: CAC85303.1. Z28292 Genomic DNA. Translation: CAA82146.1. BK006944 Genomic DNA. Translation: DAA09218.1.
PIR	S38143.
RefSeq	NP_012993.1. NM_001179857.1.
3D structure databases
ProteinModelPortal	P36148.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6620N.
IntAct	P36148. 47 interactions.
MINT	MINT-643778.
STRING	P36148.
Proteomic databases
PeptideAtlas	P36148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR067W; YKR067W; YKR067W.
GeneID	853941.
KEGG	sce:YKR067W.
NMPDR	fig\|4932.3.peg.3979.
Organism-specific databases
CYGD	YKR067w.
SGD	S000001775. GPT2.
Phylogenomic databases
eggNOG	fuNOG05103.
GeneTree	EFGT00050000005473.
HOGENOM	HBG324690.
OMA	FFREIKV.
OrthoDB	EOG4DFSWX.
Enzyme and pathway databases
BRENDA	2.3.1.15. 984.
Gene expression databases
ArrayExpress	P36148.
Genevestigator	P36148.
GermOnline	YKR067W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
KO	K13507.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975330.

Entry information

Entry name

GPT2_YEAST

Accession

Primary (citable) accession number: P36148
Secondary accession number(s): D6VXC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents