P36147 (PAM17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Presequence translocated-associated motor subunit PAM17, mitochondrial

Gene names

Name:	PAM17
Synonyms:	FMP18
Ordered Locus Names:	YKR065C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to organize PAM16-PAM18 (TIM16-TIM14) heterodimer. Ref.5
Subunit structure	Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18/TIM14.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.3 Ref.5.
Miscellaneous	Present with 3000 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the PAM17 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial matrix Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW presequence translocase-associated import motor Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-26467,EBI-2042717

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 14	14	Mitochondrion Potential
	Chain	15 – 197	183	Presequence translocated-associated motor subunit PAM17, mitochondrial		PRO_0000043179
Regions
	Transmembrane	64 – 84	21	Helical; Potential
	Transmembrane	103 – 123	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P36147 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 633C74D569E51603	FASTA	197	21,968
	10 20 30 40 50 60 MFTSAIRLSS QRLFASQPSV TAAALRSTAT TLPLRSYSQP ASLQDSSILT WSDFFKLRKQ 70 80 90 100 110 120 QRRINVGSSL FTALLGCNVS WAYLSTMEID PTQMLFGFDP LTVISAGIIA SGALGYLLGP 130 140 150 160 170 180 IVGSQVFKLS HNQQLAQFNN KNKEFLKHII NNRVDASSQS FSNPVPDYYG EKIGSLKEYK 190 QWLRDCHAYA KKAKEFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor." van der Laan M., Chacinska A., Lind M., Perschil I., Sickmann A., Meyer H.E., Guiard B., Meisinger C., Pfanner N., Rehling P. Mol. Cell. Biol. 25:7449-7458(2005) [PubMed: 16107694] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PAM COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28290 Genomic DNA. Translation: CAA82144.1. BK006944 Genomic DNA. Translation: DAA09216.1.
PIR	S38141.
RefSeq	NP_012991.1. NM_001179855.1.
3D structure databases
ProteinModelPortal	P36147.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2128N.
IntAct	P36147. 2 interactions.
MINT	MINT-547403.
STRING	P36147.
Proteomic databases
PeptideAtlas	P36147.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR065C; YKR065C; YKR065C.
GeneID	853939.
KEGG	sce:YKR065C.
NMPDR	fig|4932.3.peg.3977.
Organism-specific databases
CYGD	YKR065c.
SGD	S000001773. PAM17.
Phylogenomic databases
eggNOG	fuNOG09299.
GeneTree	EFGT00050000003497.
HOGENOM	HBG396609.
OMA	DVEKPIM.
OrthoDB	EOG46T6B1.
Gene expression databases
ArrayExpress	P36147.
Genevestigator	P36147.
GermOnline	YKR065C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013875. Pam17. [Graphical view]
Pfam	PF08566. Pam17. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975324.

Entry information

Entry name

PAM17_YEAST

Accession

Primary (citable) accession number: P36147
Secondary accession number(s): D6VXC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program