ID   RL1D1_YEAST             Reviewed;         274 AA.
AC   P36144; D6VXC1;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   27-MAR-2024, entry version 163.
DE   RecName: Full=Ribosome biogenesis protein UTP30;
DE   AltName: Full=U3 snoRNP-associated protein UTP30;
GN   Name=UTP30; OrderedLocusNames=YKR060W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   IDENTIFICATION IN THE 90S PRE-RIBOSOME, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RX   PubMed=12150911; DOI=10.1016/s1097-2765(02)00579-8;
RA   Grandi P., Rybin V., Bassler J., Petfalski E., Strauss D., Marzioch M.,
RA   Schaefer T., Kuster B., Tschochner H., Tollervey D., Gavin A.-C., Hurt E.;
RT   "90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit
RT   processing factors but predominantly lack 60S synthesis factors.";
RL   Mol. Cell 10:105-115(2002).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   INTERACTION WITH FAF1.
RX   PubMed=16762320; DOI=10.1016/j.bbrc.2006.05.140;
RA   Rempola B., Karkusiewicz I., Piekarska I., Rytka J.;
RT   "Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins
RT   involved in pre-rRNA processing.";
RL   Biochem. Biophys. Res. Commun. 346:546-554(2006).
RN   [8]
RP   FUNCTION.
RX   PubMed=16544271; DOI=10.1002/yea.1353;
RA   Wade C.H., Umbarger M.A., McAlear M.A.;
RT   "The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains
RT   over 200 genes.";
RL   Yeast 23:293-306(2006).
CC   -!- FUNCTION: Involved in rRNA-processing and ribosome biosynthesis.
CC       {ECO:0000269|PubMed:16544271}.
CC   -!- SUBUNIT: Component of the 90S pre-ribosomes. Interacts with FAF1.
CC       {ECO:0000269|PubMed:12150911, ECO:0000269|PubMed:16762320}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 3060 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL1 family.
CC       Highly divergent. {ECO:0000305}.
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DR   EMBL; Z28285; CAA82139.1; -; Genomic_DNA.
DR   EMBL; AY557907; AAS56233.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09211.1; -; Genomic_DNA.
DR   PIR; S38136; S38136.
DR   RefSeq; NP_012986.1; NM_001179850.1.
DR   PDB; 5WLC; EM; 3.80 A; SN=1-274.
DR   PDB; 5WYJ; EM; 8.70 A; U5=1-274.
DR   PDB; 5WYK; EM; 4.50 A; U5=1-274.
DR   PDB; 5YDT; EM; 4.50 A; U5=1-274.
DR   PDB; 5YDU; X-ray; 2.65 A; A/B=1-274.
DR   PDB; 6KE6; EM; 3.40 A; RI=1-274.
DR   PDB; 6LQP; EM; 3.20 A; RI=1-274.
DR   PDB; 6LQU; EM; 3.70 A; RI=1-274.
DR   PDB; 6ZQA; EM; 4.40 A; UZ=1-274.
DR   PDB; 6ZQB; EM; 3.90 A; UZ=1-274.
DR   PDB; 6ZQC; EM; 3.80 A; UZ=1-274.
DR   PDB; 7AJT; EM; 4.60 A; UZ=1-274.
DR   PDB; 7D5S; EM; 4.60 A; RI=1-274.
DR   PDB; 7SUK; EM; 3.99 A; SN=11-257.
DR   PDBsum; 5WLC; -.
DR   PDBsum; 5WYJ; -.
DR   PDBsum; 5WYK; -.
DR   PDBsum; 5YDT; -.
DR   PDBsum; 5YDU; -.
DR   PDBsum; 6KE6; -.
DR   PDBsum; 6LQP; -.
DR   PDBsum; 6LQU; -.
DR   PDBsum; 6ZQA; -.
DR   PDBsum; 6ZQB; -.
DR   PDBsum; 6ZQC; -.
DR   PDBsum; 7AJT; -.
DR   PDBsum; 7D5S; -.
DR   PDBsum; 7SUK; -.
DR   AlphaFoldDB; P36144; -.
DR   EMDB; EMD-0949; -.
DR   EMDB; EMD-0954; -.
DR   EMDB; EMD-11357; -.
DR   EMDB; EMD-11358; -.
DR   EMDB; EMD-11359; -.
DR   EMDB; EMD-11807; -.
DR   EMDB; EMD-25441; -.
DR   EMDB; EMD-30584; -.
DR   EMDB; EMD-6695; -.
DR   EMDB; EMD-6696; -.
DR   EMDB; EMD-8859; -.
DR   EMDB; EMD-9964; -.
DR   SMR; P36144; -.
DR   BioGRID; 34191; 157.
DR   ComplexPortal; CPX-1604; Small ribosomal subunit processome.
DR   DIP; DIP-1926N; -.
DR   IntAct; P36144; 18.
DR   MINT; P36144; -.
DR   STRING; 4932.YKR060W; -.
DR   iPTMnet; P36144; -.
DR   MaxQB; P36144; -.
DR   PaxDb; 4932-YKR060W; -.
DR   PeptideAtlas; P36144; -.
DR   EnsemblFungi; YKR060W_mRNA; YKR060W; YKR060W.
DR   GeneID; 853934; -.
DR   KEGG; sce:YKR060W; -.
DR   AGR; SGD:S000001768; -.
DR   SGD; S000001768; UTP30.
DR   VEuPathDB; FungiDB:YKR060W; -.
DR   eggNOG; KOG1685; Eukaryota.
DR   HOGENOM; CLU_063901_0_0_1; -.
DR   InParanoid; P36144; -.
DR   OMA; CLTVRIG; -.
DR   OrthoDB; 103361at2759; -.
DR   BioCyc; YEAST:G3O-32028-MONOMER; -.
DR   BioGRID-ORCS; 853934; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P36144; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P36144; Protein.
DR   GO; GO:0030686; C:90S preribosome; IDA:GO_Central.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0005730; C:nucleolus; IDA:GO_Central.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0032040; C:small-subunit processome; IPI:ComplexPortal.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; NAS:ComplexPortal.
DR   GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IGI:GO_Central.
DR   GO; GO:0042274; P:ribosomal small subunit biogenesis; IPI:SGD.
DR   InterPro; IPR023674; Ribosomal_uL1-like.
DR   InterPro; IPR028364; Ribosomal_uL1/biogenesis.
DR   Pfam; PF00687; Ribosomal_L1; 1.
DR   SUPFAM; SSF56808; Ribosomal protein L1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Nucleus; Reference proteome; Ribosome biogenesis;
KW   rRNA processing.
FT   CHAIN           1..274
FT                   /note="Ribosome biogenesis protein UTP30"
FT                   /id="PRO_0000203217"
FT   HELIX           12..26
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           28..30
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          35..44
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          55..57
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           67..69
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          72..79
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           81..88
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   TURN            91..96
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          98..102
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           103..109
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           112..116
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          123..128
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           129..134
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          152..154
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           173..185
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          186..189
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          195..204
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           209..223
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   HELIX           226..228
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   TURN            229..231
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          239..245
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          247..249
FT                   /evidence="ECO:0007829|PDB:5YDU"
FT   STRAND          252..255
FT                   /evidence="ECO:0007829|PDB:5YDU"
SQ   SEQUENCE   274 AA;  31616 MW;  897F2B8C54CE8030 CRC64;
     MVESNDIIKS GLAEKALKAL ILQCEENPSL KNDKDIHIII NTGKKMGINR DNIPRIIPLT
     KYKLFKPRDL NILLITKDPS ALYRETLTKD EHTSELFKEI ISVKNLRRRF KGSKLTQLYK
     DFDLVVADYR VHHLLPEVLG SRFYHGSKKL PYMIRMSKEV KLKRQQMVEK CDPIYVRAQL
     RSICKNTSYI PNNDNCLSVR VGYIQKHSIP EILQNIQDTI NFLTDKSKRP QGGVIKGGII
     SIFVKTSNST SLPIYQFSEA RENQKNEDLS DIKL
//