P36144 (RL1D1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome biogenesis protein UTP30

Alternative name(s):
U3 snoRNP-associated protein UTP30

Gene names

Name:	UTP30
Ordered Locus Names:	YKR060W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in rRNA-processing and ribosome biosynthesis. Ref.8
Subunit structure	Component of the 90S pre-ribosomes. Interacts with FAF1. Ref.4 Ref.7
Subcellular location	Nucleus › nucleolus Ref.5.
Miscellaneous	Present with 3060 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein L1P family. Highly divergent.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW ribosomal small subunit biogenesis Inferred from physical interaction PubMed 21724601. Source: SGD translation Inferred from electronic annotation. Source: InterPro
Cellular_component	90S preribosome Inferred from direct assay Ref.4. Source: SGD large ribosomal subunit Inferred from electronic annotation. Source: InterPro nucleolus Inferred from direct assay Ref.5. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Ribosome biogenesis protein UTP30		PRO_0000203217
Amino acid modifications
	Modified residue	270	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P36144 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 897F2B8C54CE8030	FASTA	274	31,616
	10 20 30 40 50 60 MVESNDIIKS GLAEKALKAL ILQCEENPSL KNDKDIHIII NTGKKMGINR DNIPRIIPLT 70 80 90 100 110 120 KYKLFKPRDL NILLITKDPS ALYRETLTKD EHTSELFKEI ISVKNLRRRF KGSKLTQLYK 130 140 150 160 170 180 DFDLVVADYR VHHLLPEVLG SRFYHGSKKL PYMIRMSKEV KLKRQQMVEK CDPIYVRAQL 190 200 210 220 230 240 RSICKNTSYI PNNDNCLSVR VGYIQKHSIP EILQNIQDTI NFLTDKSKRP QGGVIKGGII 250 260 270 SIFVKTSNST SLPIYQFSEA RENQKNEDLS DIKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors." Grandi P., Rybin V., Bassler J., Petfalski E., Strauss D., Marzioch M., Schaefer T., Kuster B., Tschochner H., Tollervey D., Gavin A.-C., Hurt E. Mol. Cell 10:105-115(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE 90S PRE-RIBOSOME, MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing." Rempola B., Karkusiewicz I., Piekarska I., Rytka J. Biochem. Biophys. Res. Commun. 346:546-554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FAF1.
[8]	"The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes." Wade C.H., Umbarger M.A., McAlear M.A. Yeast 23:293-306(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28285 Genomic DNA. Translation: CAA82139.1. AY557907 Genomic DNA. Translation: AAS56233.1. BK006944 Genomic DNA. Translation: DAA09211.1.
PIR	S38136.
RefSeq	NP_012986.1. NM_001179850.1.
3D structure databases
ProteinModelPortal	P36144.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1926N.
IntAct	P36144. 12 interactions.
MINT	MINT-8285317.
STRING	4932.YKR060W.
Proteomic databases
PaxDb	P36144.
PeptideAtlas	P36144.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR060W; YKR060W; YKR060W.
GeneID	853934.
KEGG	sce:YKR060W.
Organism-specific databases
CYGD	YKR060w.
SGD	S000001768. UTP30.
Phylogenomic databases
eggNOG	NOG316750.
HOGENOM	HOG000248442.
KO	K14775.
OMA	DPSTPYR.
OrthoDB	EOG41ZJM5.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32028-MONOMER.
Gene expression databases
Genevestigator	P36144.
GermOnline	YKR060W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002143. Ribosomal_L1. IPR023674. Ribosomal_L1_SF. [Graphical view]
Pfam	PF00687. Ribosomal_L1. 1 hit. [Graphical view]
SUPFAM	SSF56808. Ribosomal_L1. 1 hit.
ProtoNet	Search...
Other
NextBio	975309.

Entry information

Entry name

RL1D1_YEAST

Accession

Primary (citable) accession number: P36144
Secondary accession number(s): D6VXC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program