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P36143

- GLG1_YEAST

UniProt

P36143 - GLG1_YEAST

Protein

Glycogenin-1

Gene

GLG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 4 (20 May 2008)
      Previous versions | rss
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    Functioni

    Self-glucosylating initiator of glycogen synthesis. Catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues. These chains act as primers for the elongation reaction catalyzed by glycogen synthase. Capable of transferring glucosyl residues to unbound acceptors such as free oligoglucans or oligoglucan derivatives.2 Publications

    Catalytic activityi

    UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

    GO - Molecular functioni

    1. glycogenin glucosyltransferase activity Source: SGD

    GO - Biological processi

    1. glycogen biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:MONOMER3O-4054.

    Protein family/group databases

    CAZyiGT8. Glycosyltransferase Family 8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogenin-1 (EC:2.4.1.186)
    Alternative name(s):
    Glycogen synthesis initiator protein 1
    Glycogenin glucosyltransferase 1
    Gene namesi
    Name:GLG1
    Ordered Locus Names:YKR058W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    SGDiS000001766. GLG1.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi230 – 2301Y → F: Eliminates glycogen accumulation; when associated with F-598. 1 Publication
    Mutagenesisi598 – 5981Y → F: Eliminates glycogen accumulation; when associated with F-230. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 616616Glycogenin-1PRO_0000215181Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi230 – 2301O-linked (Glc...)By similarity
    Glycosylationi598 – 5981O-linked (Glc...)Curated

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP36143.
    PaxDbiP36143.
    PeptideAtlasiP36143.

    Expressioni

    Inductioni

    Induced during the diauxic transition.1 Publication

    Gene expression databases

    GenevestigatoriP36143.

    Interactioni

    Protein-protein interaction databases

    BioGridi34189. 34 interactions.
    IntActiP36143. 2 interactions.
    MINTiMINT-2779894.
    STRINGi4932.YKR058W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36143.
    SMRiP36143. Positions 55-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG321063.
    GeneTreeiENSGT00390000004721.
    HOGENOMiHOG000112734.
    OMAiHFIGKHK.
    OrthoDBiEOG7BS4K4.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR002495. Glyco_trans_8.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF01501. Glyco_transf_8. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36143-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKKLAIATL LYSADYLPGV FALGHQVNKL LEEAGKKGDI ETCLIVTTSL    50
    FNGTLSELAK NILQSIYTKI VLVEPLNCQE ESIQKNSENL ALLERPELSF 100
    ALIKARLWEL TQFEQVLYLD SDTLPLNKEF LKLFDIMSKQ TTSQVGAIAD 150
    IGWPDMFNSG VMMLIPDADT ASVLQNYIFE NTSIDGSDQG ILNQFFNQNC 200
    CTDELVKDSF SREWVQLSFT YNVTIPNLGY QSSPAMNYFK PSIKLIHFIG 250
    KHKPWSLWSQ KNFIKNEYHD QWNEVYEEFK EEHQLNNEVS KPKISDSDKT 300
    ETPETITPVD APPSNEPTTN QEIDTISTVE ENVDNQNAEP VPNSDHSPAP 350
    NPVPLDFTKW LTTFINKDHL TNQPVNESRE YSKENDNNII NSSSNRDQES 400
    PPNSTQELNS SYSVVSTQAD SDEHQNAEEE DSTTDNASNS GEESHLDDIS 450
    TAASSNNNVS NQPDGKNFSN SKENNISVES SPSNPEQKRS TDNIQKPSVS 500
    TNDLPDDVEP HTSVDDNIQY LEKDKEGYEE FLPDVYESNA IDNEEEFFDD 550
    DARDATEGET KTSAVADKQE DMKLTAEETN QPQQEMPNFK FDWEDSDYLS 600
    KVERCFPDDI FEYAVE 616
    Length:616
    Mass (Da):69,724
    Last modified:May 20, 2008 - v4
    Checksum:i35DFF4A1E043FC8B
    GO

    Sequence cautioni

    The sequence CAA82136.1 differs from that shown. Reason: Frameshift at positions 6 and 38.
    The sequence AAA91646.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA82136.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25546 Genomic DNA. Translation: AAA91646.1. Different initiation.
    Z28283 Genomic DNA. Translation: CAA82136.1. Sequence problems.
    BK006944 Genomic DNA. Translation: DAA09209.1.
    PIRiS38134.
    RefSeqiNP_012984.2. NM_001179848.1.

    Genome annotation databases

    EnsemblFungiiYKR058W; YKR058W; YKR058W.
    GeneIDi853932.
    KEGGisce:YKR058W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25546 Genomic DNA. Translation: AAA91646.1 . Different initiation.
    Z28283 Genomic DNA. Translation: CAA82136.1 . Sequence problems.
    BK006944 Genomic DNA. Translation: DAA09209.1 .
    PIRi S38134.
    RefSeqi NP_012984.2. NM_001179848.1.

    3D structure databases

    ProteinModelPortali P36143.
    SMRi P36143. Positions 55-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34189. 34 interactions.
    IntActi P36143. 2 interactions.
    MINTi MINT-2779894.
    STRINGi 4932.YKR058W.

    Protein family/group databases

    CAZyi GT8. Glycosyltransferase Family 8.

    Proteomic databases

    MaxQBi P36143.
    PaxDbi P36143.
    PeptideAtlasi P36143.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR058W ; YKR058W ; YKR058W .
    GeneIDi 853932.
    KEGGi sce:YKR058W.

    Organism-specific databases

    SGDi S000001766. GLG1.

    Phylogenomic databases

    eggNOGi NOG321063.
    GeneTreei ENSGT00390000004721.
    HOGENOMi HOG000112734.
    OMAi HFIGKHK.
    OrthoDBi EOG7BS4K4.

    Enzyme and pathway databases

    BioCyci YEAST:MONOMER3O-4054.

    Miscellaneous databases

    NextBioi 975303.

    Gene expression databases

    Genevestigatori P36143.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR002495. Glyco_trans_8.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF01501. Glyco_transf_8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae."
      Cheng C., Mu J., Farkas I., Huang D., Goebl M.G., Roach P.J.
      Mol. Cell. Biol. 15:6632-6640(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Initiation of glycogen synthesis in yeast. Requirement of multiple tyrosine residues for function of the self-glucosylating Glg proteins in vivo."
      Mu J., Cheng C., Roach P.J.
      J. Biol. Chem. 271:26554-26560(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TYR-230 AND TYR-598.
    5. "Dynamic responses of reserve carbohydrate metabolism under carbon and nitrogen limitations in Saccharomyces cerevisiae."
      Parrou J.L., Enjalbert B., Plourde L., Bauche A., Gonzalez B., Francois J.
      Yeast 15:191-203(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
      Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
      Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    7. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
      Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
      Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLG1_YEAST
    AccessioniPrimary (citable) accession number: P36143
    Secondary accession number(s): D6VXB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 94 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3