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P36143 (GLG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogenin-1

EC=2.4.1.186
Alternative name(s):
Glycogen synthesis initiator protein 1
Glycogenin glucosyltransferase 1
Gene names
Name:GLG1
Ordered Locus Names:YKR058W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length616 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Self-glucosylating initiator of glycogen synthesis. Catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues. These chains act as primers for the elongation reaction catalyzed by glycogen synthase. Capable of transferring glucosyl residues to unbound acceptors such as free oligoglucans or oligoglucan derivatives. Ref.1 Ref.4

Catalytic activity

UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

Induction

Induced during the diauxic transition. Ref.5

Sequence similarities

Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.

Sequence caution

The sequence AAA91646.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA82136.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAA82136.1 differs from that shown. Reason: Frameshift at positions 6 and 38.

Ontologies

Keywords
   Biological processGlycogen biosynthesis
   Molecular functionTransferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycogen biosynthetic process

Inferred from genetic interaction Ref.1. Source: SGD

   Molecular_functionglycogenin glucosyltransferase activity

Inferred from genetic interaction Ref.1. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 616616Glycogenin-1
PRO_0000215181

Amino acid modifications

Glycosylation2301O-linked (Glc...) By similarity
Glycosylation5981O-linked (Glc...) Probable

Experimental info

Mutagenesis2301Y → F: Eliminates glycogen accumulation; when associated with F-598. Ref.4
Mutagenesis5981Y → F: Eliminates glycogen accumulation; when associated with F-230. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P36143 [UniParc].

Last modified May 20, 2008. Version 4.
Checksum: 35DFF4A1E043FC8B

FASTA61669,724
        10         20         30         40         50         60 
MYKKLAIATL LYSADYLPGV FALGHQVNKL LEEAGKKGDI ETCLIVTTSL FNGTLSELAK 

        70         80         90        100        110        120 
NILQSIYTKI VLVEPLNCQE ESIQKNSENL ALLERPELSF ALIKARLWEL TQFEQVLYLD 

       130        140        150        160        170        180 
SDTLPLNKEF LKLFDIMSKQ TTSQVGAIAD IGWPDMFNSG VMMLIPDADT ASVLQNYIFE 

       190        200        210        220        230        240 
NTSIDGSDQG ILNQFFNQNC CTDELVKDSF SREWVQLSFT YNVTIPNLGY QSSPAMNYFK 

       250        260        270        280        290        300 
PSIKLIHFIG KHKPWSLWSQ KNFIKNEYHD QWNEVYEEFK EEHQLNNEVS KPKISDSDKT 

       310        320        330        340        350        360 
ETPETITPVD APPSNEPTTN QEIDTISTVE ENVDNQNAEP VPNSDHSPAP NPVPLDFTKW 

       370        380        390        400        410        420 
LTTFINKDHL TNQPVNESRE YSKENDNNII NSSSNRDQES PPNSTQELNS SYSVVSTQAD 

       430        440        450        460        470        480 
SDEHQNAEEE DSTTDNASNS GEESHLDDIS TAASSNNNVS NQPDGKNFSN SKENNISVES 

       490        500        510        520        530        540 
SPSNPEQKRS TDNIQKPSVS TNDLPDDVEP HTSVDDNIQY LEKDKEGYEE FLPDVYESNA 

       550        560        570        580        590        600 
IDNEEEFFDD DARDATEGET KTSAVADKQE DMKLTAEETN QPQQEMPNFK FDWEDSDYLS 

       610 
KVERCFPDDI FEYAVE 

« Hide

References

« Hide 'large scale' references
[1]"Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae."
Cheng C., Mu J., Farkas I., Huang D., Goebl M.G., Roach P.J.
Mol. Cell. Biol. 15:6632-6640(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Initiation of glycogen synthesis in yeast. Requirement of multiple tyrosine residues for function of the self-glucosylating Glg proteins in vivo."
Mu J., Cheng C., Roach P.J.
J. Biol. Chem. 271:26554-26560(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TYR-230 AND TYR-598.
[5]"Dynamic responses of reserve carbohydrate metabolism under carbon and nitrogen limitations in Saccharomyces cerevisiae."
Parrou J.L., Enjalbert B., Plourde L., Bauche A., Gonzalez B., Francois J.
Yeast 15:191-203(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]"Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25546 Genomic DNA. Translation: AAA91646.1. Different initiation.
Z28283 Genomic DNA. Translation: CAA82136.1. Sequence problems.
BK006944 Genomic DNA. Translation: DAA09209.1.
PIRS38134.
RefSeqNP_012984.2. NM_001179848.1.

3D structure databases

ProteinModelPortalP36143.
SMRP36143. Positions 55-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34189. 34 interactions.
IntActP36143. 2 interactions.
MINTMINT-2779894.
STRING4932.YKR058W.

Protein family/group databases

CAZyGT8. Glycosyltransferase Family 8.

Proteomic databases

MaxQBP36143.
PaxDbP36143.
PeptideAtlasP36143.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR058W; YKR058W; YKR058W.
GeneID853932.
KEGGsce:YKR058W.

Organism-specific databases

SGDS000001766. GLG1.

Phylogenomic databases

eggNOGNOG321063.
GeneTreeENSGT00390000004721.
HOGENOMHOG000112734.
OMAHFIGKHK.
OrthoDBEOG7BS4K4.

Enzyme and pathway databases

BioCycYEAST:MONOMER3O-4054.

Gene expression databases

GenevestigatorP36143.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio975303.

Entry information

Entry nameGLG1_YEAST
AccessionPrimary (citable) accession number: P36143
Secondary accession number(s): D6VXB9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 20, 2008
Last modified: June 11, 2014
This is version 93 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families