ID   YK25_YEAST              Reviewed;         183 AA.
AC   P36138; D6VXA7;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 2.
DT   08-NOV-2023, entry version 120.
DE   RecName: Full=Uncharacterized protein YKR045C;
GN   OrderedLocusNames=YKR045C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=11914276; DOI=10.1101/gad.970902;
RA   Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S.,
RA   Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P.,
RA   Gerstein M., Roeder G.S., Snyder M.;
RT   "Subcellular localization of the yeast proteome.";
RL   Genes Dev. 16:707-719(2002).
RN   [5]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [6]
RP   UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-21, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22106047; DOI=10.1002/pmic.201100166;
RA   Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT   "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL   Proteomics 12:236-240(2012).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11914276}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAS56505.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA82121.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z28270; CAA82121.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AY558179; AAS56505.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BK006944; DAA09197.1; -; Genomic_DNA.
DR   PIR; S38117; S38117.
DR   RefSeq; NP_012971.2; NM_001179835.1.
DR   AlphaFoldDB; P36138; -.
DR   BioGRID; 34177; 50.
DR   STRING; 4932.YKR045C; -.
DR   iPTMnet; P36138; -.
DR   MaxQB; P36138; -.
DR   PaxDb; 4932-YKR045C; -.
DR   PeptideAtlas; P36138; -.
DR   EnsemblFungi; YKR045C_mRNA; YKR045C; YKR045C.
DR   GeneID; 853919; -.
DR   KEGG; sce:YKR045C; -.
DR   AGR; SGD:S000001753; -.
DR   SGD; S000001753; YKR045C.
DR   VEuPathDB; FungiDB:YKR045C; -.
DR   eggNOG; ENOG502SAAB; Eukaryota.
DR   HOGENOM; CLU_083988_0_0_1; -.
DR   InParanoid; P36138; -.
DR   OMA; PSMSNSH; -.
DR   OrthoDB; 2032790at2759; -.
DR   BioCyc; YEAST:G3O-32016-MONOMER; -.
DR   BioGRID-ORCS; 853919; 4 hits in 10 CRISPR screens.
DR   PRO; PR:P36138; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P36138; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
PE   1: Evidence at protein level;
KW   Cytoplasm; Isopeptide bond; Reference proteome; Ubl conjugation.
FT   CHAIN           1..183
FT                   /note="Uncharacterized protein YKR045C"
FT                   /id="PRO_0000203213"
FT   REGION          24..111
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          160..183
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..41
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..72
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        73..92
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        95..111
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        21
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0007744|PubMed:22106047"
SQ   SEQUENCE   183 AA;  20226 MW;  5858E2176C314148 CRC64;
     MSNSHHTSQG RRNKLSVWVK KIINTTTTTN ASVSSSKPRR GTRAGPTRVK RAELDPDGTT
     ISSSLRPLVD RNSLHSSESD DEGDRRVAWD EPPTGKVRQQ QQQQQQQQND NASVIPLVSF
     CSSSVKSSTF SDIHSIQSTR PTIFSNRTFE TNSSVLAIPP QSILDRSRTL PPSNASNTTT
     RRP
//