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P36135

- UTH1_YEAST

UniProt

P36135 - UTH1_YEAST

Protein

Probable secreted beta-glucosidase UTH1

Gene

UTH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Involved in aging, oxidative stress response, and in the regulation of mitochondrial biogenesis. Inactivation of UTH1 increases life span, leads to higher resistance to heat stress and against hydrogen peroxide, and increases sensitivity to the superoxide radical-generating drug paraquat and to copper. Also required for the selective autophagic degradation of mitochondria (mitophagy) in response to nitrogen starvation. Involved in the remodeling of the cell wall during the various phases of yeast culture development and under various environmental conditions and plays a role in septation. Involved in cell sensitivity to boric acid.8 Publications

    GO - Molecular functioni

    1. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW

    GO - Biological processi

    1. barrier septum assembly Source: UniProtKB-KW
    2. fungal-type cell wall biogenesis Source: SGD
    3. mitochondrion degradation Source: SGD
    4. negative regulation of chromatin silencing involved in replicative cell aging Source: SGD
    5. polysaccharide catabolic process Source: UniProtKB-KW
    6. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Autophagy, Carbohydrate metabolism, Cell cycle, Cell division, Cell wall biogenesis/degradation, Polysaccharide degradation, Septation, Stress response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32013-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable secreted beta-glucosidase UTH1 (EC:3.2.1.-)
    Alternative name(s):
    Youth protein 1
    Gene namesi
    Name:UTH1
    Ordered Locus Names:YKR042W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    SGDiS000001750. UTH1.

    Subcellular locationi

    Mitochondrion outer membrane; Peripheral membrane protein. Secretedcell wall
    Note: Non-covalently bound to the cell wall.

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW
    3. extrinsic component of mitochondrial inner membrane Source: SGD
    4. mitochondrial outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell wall, Membrane, Mitochondrion, Mitochondrion outer membrane, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Leads to increased resistance to zymolyase treatment and to boric acid.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 365348Probable secreted beta-glucosidase UTH1PRO_0000194002Add
    BLAST

    Proteomic databases

    MaxQBiP36135.
    PaxDbiP36135.
    PeptideAtlasiP36135.

    Miscellaneous databases

    PMAP-CutDBP36135.

    Expressioni

    Inductioni

    Induced by hydrogen peroxide. Repressed by superoxide radicals and anoxia.2 Publications

    Gene expression databases

    GenevestigatoriP36135.

    Interactioni

    Protein-protein interaction databases

    BioGridi34174. 123 interactions.
    MINTiMINT-2782317.
    STRINGi4932.YKR042W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36135.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi89 – 10315Poly-SerAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SUN family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG83991.
    GeneTreeiENSGT00390000002934.
    HOGENOMiHOG000180870.
    OMAiGCIWGTD.
    OrthoDBiEOG79PK14.

    Family and domain databases

    InterProiIPR005556. SUN.
    [Graphical view]
    PfamiPF03856. SUN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36135-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSALLALS ASTAVLAAPA VHHSDNHHHN DKRAVVTVTQ YVNADGAVVI    50
    PAATTATSAA ADGKVESVAA ATTTLSSTAA AATTSAAASS SSSSSSSSSS 100
    SSSVGSGDFE DGTISCSDFP SGQGAVSLDW LGLGGWASIM DMNGNTATSC 150
    QDGYYCSYAC SPGYAKTQWP SEQPSDGRSV GGLYCKNGKL YRSNTDTNSL 200
    CVEGQGSAQA VNKVSGSIAI CGTDYPGSEN MVVPTVVGAG SSQPINVIKE 250
    DSYYQWQGKK TSAQYYVNNA GVSVEDGCIW GTEGSGVGNW APVVLGAGYT 300
    DGITYLSIIP NPNNKEAPNF NIKIVATDGS TVNGACSYEN GVYSGSGSDG 350
    CTVSVTSGSA NFVFY 365
    Length:365
    Mass (Da):36,955
    Last modified:June 16, 2009 - v2
    Checksum:i5DBAB73510FA6F84
    GO

    Sequence cautioni

    The sequence CAA82118.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28267 Genomic DNA. Translation: CAA82118.1. Different initiation.
    BK006944 Genomic DNA. Translation: DAA09194.1.
    PIRiS38114.
    RefSeqiNP_012968.4. NM_001179832.3.

    Genome annotation databases

    EnsemblFungiiYKR042W; YKR042W; YKR042W.
    GeneIDi853916.
    KEGGisce:YKR042W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28267 Genomic DNA. Translation: CAA82118.1 . Different initiation.
    BK006944 Genomic DNA. Translation: DAA09194.1 .
    PIRi S38114.
    RefSeqi NP_012968.4. NM_001179832.3.

    3D structure databases

    ProteinModelPortali P36135.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34174. 123 interactions.
    MINTi MINT-2782317.
    STRINGi 4932.YKR042W.

    Proteomic databases

    MaxQBi P36135.
    PaxDbi P36135.
    PeptideAtlasi P36135.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR042W ; YKR042W ; YKR042W .
    GeneIDi 853916.
    KEGGi sce:YKR042W.

    Organism-specific databases

    SGDi S000001750. UTH1.

    Phylogenomic databases

    eggNOGi NOG83991.
    GeneTreei ENSGT00390000002934.
    HOGENOMi HOG000180870.
    OMAi GCIWGTD.
    OrthoDBi EOG79PK14.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32013-MONOMER.

    Miscellaneous databases

    NextBioi 975258.
    PMAP-CutDB P36135.

    Gene expression databases

    Genevestigatori P36135.

    Family and domain databases

    InterProi IPR005556. SUN.
    [Graphical view ]
    Pfami PF03856. SUN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Mutation in the silencing gene SIR4 can delay aging in S.cerevisiae."
      Kennedy B.K., Austriaco N.R. Jr., Zhang J., Guarente L.
      Cell 80:485-496(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "Involvement of the Saccharomyces cerevisiae UTH1 gene in the oxidative-stress response."
      Bandara P.D.S., Flattery-O'Brien J.A., Grant C.M., Dawes I.W.
      Curr. Genet. 34:259-268(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, INDUCTION, FUNCTION.
    5. "The 'SUN' family: UTH1, an ageing gene, is also involved in the regulation of mitochondria biogenesis in Saccharomyces cerevisiae."
      Camougrand N.M., Mouassite M., Velours G.M., Guerin M.G.
      Arch. Biochem. Biophys. 375:154-160(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The SUN family of Saccharomyces cerevisiae: the double knock-out of UTH1 and SIM1 promotes defects in nucleus migration and increased drug sensitivity."
      Mouassite M., Guerin M.G., Camougrand N.M.
      FEMS Microbiol. Lett. 182:137-141(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The 'SUN' family: yeast SUN4/SCW3 is involved in cell septation."
      Mouassite M., Camougrand N.M., Schwob E., Demaison G., Laclau M., Guerin M.G.
      Yeast 16:905-919(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Dual cell wall/mitochondria localization of the 'SUN' family proteins."
      Velours G.M., Boucheron C., Manon S., Camougrand N.M.
      FEMS Microbiol. Lett. 207:165-172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "The product of the UTH1 gene, required for Bax-induced cell death in yeast, is involved in the response to rapamycin."
      Camougrand N.M., Grelaud-Coq A., Marza E., Priault M., Bessoule J.-J., Manon S.
      Mol. Microbiol. 47:495-506(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "Uth1p is involved in the autophagic degradation of mitochondria."
      Kissova I., Deffieu M., Manon S., Camougrand N.M.
      J. Biol. Chem. 279:39068-39074(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
      Zhang Z., Dietrich F.S.
      Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    14. "Selective and non-selective autophagic degradation of mitochondria in yeast."
      Kissova I., Salin B., Schaeffer J., Bhatia S., Manon S., Camougrand N.M.
      Autophagy 3:329-336(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces cerevisiae and produced dependently on oxygen level."
      Kuznetsov E., Kucerova H., Vachova L., Palkova Z.
      PLoS ONE 8:E73882-E73882(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry nameiUTH1_YEAST
    AccessioniPrimary (citable) accession number: P36135
    Secondary accession number(s): D6VXA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 56900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3