DASH complex subunit SPC34 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36131 (SPC34_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DASH complex subunit SPC34

Alternative name(s):
34 kDa spindle pole component protein
Outer kinetochore protein SPC34

Gene names

Name:	SPC34
Ordered Locus Names:	YKR037C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. Ref.6 Ref.7 Ref.9
Subunit structure	The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1. Ref.8
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle. Chromosome › centromere › kinetochore. Note: Associates with the mitotic spindle and the kinetochore. Ref.5
Sequence similarities	Belongs to the DASH complex SPC34 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Microtubule Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW microtubule nucleation Inferred from physical interaction. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle organization in nucleus Inferred from physical interaction. Source: SGD regulation of microtubule polymerization or depolymerization Inferred from physical interaction. Source: SGD
Cellular component	DASH complex Inferred from direct assay. Source: SGD spindle microtubule Inferred from direct assay. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
DAM1	P53267	5	EBI-26401,EBI-23268
DUO1	P53168	3	EBI-26401,EBI-23800
SPC19	Q03954	4	EBI-26401,EBI-38809

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

DASH complex subunit SPC34

PRO_0000211554

Regions

Coiled coil

223 – 295

Potential

Amino acid modifications

Modified residue

199

Phosphothreonine; by IPL1 Ref.3 Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P36131 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: C1061B49F6D4D308
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FASTA

295

34,078

        10         20         30         40         50         60 
MGESLDRCID DINRAVDSMS TLYFKPPGIF HNAILQGASN KASIRKDITR LIKDCNHDEA 

        70         80         90        100        110        120 
YLLFKVNPEK QSVSRRDGKE GVFDYVIKRD TDMKRNRRLG RPGEKPIIHV PKEVYLNKDR 

       130        140        150        160        170        180 
LDLNNKRRRT ATTSGGGLNG FIFDTDLIGS SVISNSSSGT FKALSAVFKD DPQIQRLLYA 

       190        200        210        220        230        240 
LENGSVLMEE ESNNQRRKTI FVEDFPTDLI LKVMAEVTDL WPLTEFKQDY DQLYHNYEQL 

       250        260        270        280        290 
SSKLRFIKKE VLLQDDRLKT MSQYHPSSSH DVAKIIRKEK DEIRRLEMEI ANLQE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p." Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G. Cell 111:163-172(2002) [PubMed: 12408861] [Abstract] Cited for: PHOSPHORYLATION AT THR-199.
[4]	"The mitotic spindle is required for loading of the DASH complex onto the kinetochore." Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J. Genes Dev. 16:183-197(2002) [PubMed: 11799062] [Abstract] Cited for: COMPONENT OF THE DASH COMPLEX.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex." Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G. Mol. Cell 17:277-290(2005) [PubMed: 15664196] [Abstract] Cited for: FUNCTION.
[7]	"The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends." Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G. Nature 440:565-569(2006) [PubMed: 16415853] [Abstract] Cited for: FUNCTION.
[8]	"Molecular architecture of a kinetochore-microtubule attachment site." Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D. Nat. Cell Biol. 8:581-585(2006) [PubMed: 16715078] [Abstract] Cited for: SUBUNIT.
[9]	"The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement." Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N. Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed: 16777964] [Abstract] Cited for: FUNCTION.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-199, MASS SPECTROMETRY. Strain: ADR376.
[11]	"The yeast DASH complex forms closed rings on microtubules." Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C. Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed: 15640796] [Abstract] Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28262 Genomic DNA. Translation: CAA82111.1. BK006944 Genomic DNA. Translation: DAA09190.1.
PIR	S38109.
RefSeq	NP_012963.1. NM_001179827.1.
3D structure databases
ProteinModelPortal	P36131.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1649N.
IntAct	P36131. 12 interactions.
MINT	MINT-398351.
STRING	P36131.
Proteomic databases
PeptideAtlas	P36131.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR037C; YKR037C; YKR037C.
GeneID	853909.
KEGG	sce:YKR037C.
NMPDR	fig\|4932.3.peg.3949.
Organism-specific databases
CYGD	YKR037c.
SGD	S000001745. SPC34.
Phylogenomic databases
eggNOG	fuNOG10093.
HOGENOM	HBG202373.
OMA	YFKPPGI.
OrthoDB	EOG4V1B92.
Gene expression databases
ArrayExpress	P36131.
Genevestigator	P36131.
GermOnline	YKR037C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013966. DASH_Spc34. [Graphical view]
KO	K11573.
Pfam	PF08657. DASH_Spc34. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975242.

Entry information

Entry name

SPC34_YEAST

Accession

Primary (citable) accession number: P36131
Secondary accession number(s): D6VXA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents