P36125 (GMH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GMH1

Alternative name(s):
GEA1-6 membrane-associated high-copy suppressor protein 1

Gene names

Name:	GMH1
Synonyms:	MSG1
Ordered Locus Names:	YKR030W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with GEA1 and GEA2. Ref.3
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Recycles between Golgi apparatus and endoplasmic reticulum. Ref.3
Sequence similarities	Belongs to the unc-50 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transport Inferred from physical interaction Ref.3. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Protein GMH1		PRO_0000203205
Regions
	Topological domain	1 – 89	89	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 134	24	Lumenal Potential
	Transmembrane	135 – 155	21	Helical; Potential
	Topological domain	156 – 175	20	Cytoplasmic Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Topological domain	197 – 216	20	Lumenal Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Topological domain	238 – 242	5	Cytoplasmic Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Topological domain	264 – 273	10	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	P36125 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 94E991416D7630F2	FASTA	273	31,993
	10 20 30 40 50 60 MSYLPTYSND LPAGPQGQRR RNNGNENDAR QGYGQQSVPM VIRRLFKTPK NLDLETASWE 70 80 90 100 110 120 MFHLIFHPRK AYRSIYYQRQ TKNQWARDDP SFFIFQIALI SLSSIIWSIY NSGFNNDSDM 130 140 150 160 170 180 GALSIIGHFF KSLVMMVILD FFIFGFIMAT IFYLLLNRSH FKFKSSQNSV VEWAYCFDVH 190 200 210 220 230 240 CNSFLIILLC LYFIQFLLLP IINLQNWISL LIGNSLYCFA IGHYFILTFY GYNQLPFLKN 250 260 270 LNFILLPTLG LSIIYLISLF GIDLSKKLSF YNY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p." Chantalat S., Courbeyrette R., Senic-Matuglia F., Jackson C.L., Goud B., Peyroche A. Mol. Biol. Cell 14:2357-2371(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH GEA1 AND GEA2.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28255 Genomic DNA. Translation: CAA82102.1. BK006944 Genomic DNA. Translation: DAA09185.1.
PIR	S38102.
RefSeq	NP_012955.1. NM_001179820.1.
3D structure databases
ProteinModelPortal	P36125.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2023N.
IntAct	P36125. 8 interactions.
MINT	MINT-386053.
STRING	4932.YKR030W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR030W; YKR030W; YKR030W.
GeneID	853901.
KEGG	sce:YKR030W.
Organism-specific databases
CYGD	YKR030w.
SGD	S000001738. GMH1.
Phylogenomic databases
eggNOG	NOG262379.
GeneTree	ENSGT00390000018553.
HOGENOM	HOG000193563.
OMA	VWRAVAV.
OrthoDB	EOG4M951P.
Gene expression databases
Genevestigator	P36125.
GermOnline	YKR030W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007881. UNC-50. [Graphical view]
PANTHER	PTHR12841. PTHR12841. 1 hit.
Pfam	PF05216. UNC-50. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975227.

Entry information

Entry name

GMH1_YEAST

Accession

Primary (citable) accession number: P36125
Secondary accession number(s): D6VX95

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program