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P36123 (SA190_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SIT4-associating protein SAP190
Gene names
Name:SAP190
Ordered Locus Names:YKR028W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1033 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulator of protein phosphatase SIT4. Involved in the general amino acid control (GAAC) response regulated by TOR. Involved in the dephosphorylation of the elongator complex subunit IKI3. Ref.4 Ref.5

Subunit structure

Associates with the SIT4 protein phosphatase catalytic subunit in a cell-cycle-dependent manner.

Subcellular location

Cytoplasm Probable.

Post-translational modification

Hyperphosphorylated in the absence of SIT4. Ref.1

Sequence similarities

Belongs to the SAPS family.

Sequence caution

The sequence CAA82100.1 differs from that shown. Reason: Frameshift at position 1009.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SIT4P206044EBI-16392,EBI-13707

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10331033SIT4-associating protein SAP190
PRO_0000046107

Amino acid modifications

Modified residue7741Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue8571Phosphoserine Ref.6 Ref.8
Modified residue8621Phosphoserine Ref.8
Modified residue8921Phosphoserine Ref.8
Modified residue9901Phosphothreonine Ref.8
Modified residue9911Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P36123 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 33E5575B58B73B1A

FASTA1,033117,098
        10         20         30         40         50         60 
MSGSFWKFGQ DYSIESPVSK ILNSAFIKIN KDQDDDVPTG TCEENIADDE DNSSHDYAAS 

        70         80         90        100        110        120 
EDNVVNENEE KEEENTLPTT ESEYENYRPN LDVLDDLLDD DELYTELMCS NFKLLIFLKY 

       130        140        150        160        170        180 
PDVLSKLIEY VTNEKILDEE TDSAKKPEII EGVNDHPILI ERDRKDKKED AEEGGDSEET 

       190        200        210        220        230        240 
TNDSDHDSGD ERSVDSEETS ITLPPESEEQ VETRRARIAA EILSADVWPI SAAIMQNKDL 

       250        260        270        280        290        300 
LGRLWSILDH PAPLPIPAST YFMKINERLL DMDITGMLEF ILSRDSLVAR FLTHVDNPSL 

       310        320        330        340        350        360 
MDFLLKVIST DKPDSPTGVI KILKSQELIP KLLDHLNPEY GISTQSAAGD FIKAFVTLST 

       370        380        390        400        410        420 
NSSNELASGI GPNELTRQLV SEEMIEKLIK IMLKGGTSLS NGVGIIIELI RKNNSDYDFI 

       430        440        450        460        470        480 
QLVYTTLESH PPTDRDPIHL IHLVKLFAKH MPDFADMLDK TKLPLMEMPF GNIEPLGFER 

       490        500        510        520        530        540 
FKICELIAEL LHCSNMTLLN EPNGEMIAQE RDIERAKELE TSTEKENITA IVDNKSSYYD 

       550        560        570        580        590        600 
KDCVEKDITE NLGALQINNQ GSEEDELNDT GVSSVKLDVK SDAKVVEGLE NDASGVELYD 

       610        620        630        640        650        660 
ETLSDTESVR ECLREKPLVG DRLKIALEDT KILISILDMF TEFPWNNFLH NVIFDIAQQI 

       670        680        690        700        710        720 
FNGPLKTGYN RFLLKDYLVD AYLTKKIVDA DKACQDYEKK TGLRYGYMGH LTLVAEEISK 

       730        740        750        760        770        780 
FKEYIDEMKL TFCNTAVSDR LEEPFWKEYS ETILADTREK YNTVLGDFGN DQESDDDVIR 

       790        800        810        820        830        840 
NSDSEDIIGD TEGNENYGNG ENDELLSNGH DSGNMDLYYN FNNNENEENE EDYAEYSDVD 

       850        860        870        880        890        900 
NKNYYNNVET NDDDYDSDDG KSKSAESEFT DKISEHRDGN SLYNEDNDEN GSDKWTSGTS 

       910        920        930        940        950        960 
LFPPDHFPSR SQPSDPKLQD QNIFHHQFDF EGVGDDDDYM DPNDDGQSYA RPGNPLYTTP 

       970        980        990       1000       1010       1020 
KTPPRPKTIL FNSLSALDNN GEDEEVALGT SVDDRMDNEI SSDEEDSEDE DEENDMGNEE 

      1030 
GYSLYRSRSK EAF 

« Hide

References

« Hide 'large scale' references
[1]"The SAPs, a new family of proteins, associate and function positively with the SIT4 phosphatase."
Luke M.M., della Seta F., di Como C.J., Sugimoto H., Kobayashi R., Arndt K.T.
Mol. Cell. Biol. 16:2744-2755(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF FRAMESHIFT, INTERACTION WITH SIT4, PHOSPHORYLATION.
Strain: S288c / GRF88.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 1009.
Strain: ATCC 204508 / S288c.
[4]"The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity."
Jablonowski D., Fichtner L., Stark M.J.R., Schaffrath R.
Mol. Biol. Cell 15:1459-1469(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"TOR controls transcriptional and translational programs via Sap-Sit4 protein phosphatase signaling effectors."
Rohde J.R., Campbell S., Zurita-Martinez S.A., Cutler N.S., Ashe M., Cardenas M.E.
Mol. Cell. Biol. 24:8332-8341(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-774 AND SER-857, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-774, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-774; SER-857; SER-862; SER-892; THR-990 AND SER-991, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U50561 Genomic DNA. Translation: AAC49304.1.
Z28253 Genomic DNA. Translation: CAA82100.1. Frameshift.
BK006944 Genomic DNA. Translation: DAA09183.2.
PIRS38100.
RefSeqNP_012953.2. NM_001179818.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34161. 104 interactions.
DIPDIP-4850N.
IntActP36123. 9 interactions.
MINTMINT-518659.
STRING4932.YKR028W.

Proteomic databases

MaxQBP36123.
PaxDbP36123.
PeptideAtlasP36123.
PRIDEP36123.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR028W; YKR028W; YKR028W.
GeneID853899.
KEGGsce:YKR028W.

Organism-specific databases

SGDS000001736. SAP190.

Phylogenomic databases

eggNOGNOG303042.
GeneTreeENSGT00390000009899.
HOGENOMHOG000248046.
KOK15458.
OMACEENIAD.
OrthoDBEOG7647CP.

Enzyme and pathway databases

BioCycYEAST:G3O-32004-MONOMER.

Gene expression databases

GenevestigatorP36123.

Family and domain databases

InterProIPR007587. SAPS.
[Graphical view]
PANTHERPTHR12634. PTHR12634. 1 hit.
PfamPF04499. SAPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975221.

Entry information

Entry nameSA190_YEAST
AccessionPrimary (citable) accession number: P36123
Secondary accession number(s): D6VX93
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families