P36122 (BCH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BCH2

Alternative name(s):
BUD7 and CHS6 homolog 2

Gene names

Name:	BCH2
Synonyms:	FMP50
Ordered Locus Names:	YKR027W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the CHS5-ARF1P-binding proteins (CHAPS) which mediates export of specific cargo proteins, including chitin synthase CHS3. Ref.5 Ref.6 Ref.7
Subunit structure	Component of the CHS5/6 complex composed of the 4 CHAPS proteins BCH1, BCH2, BUD7, and CHS6 as well as at least CHS5 and GTP-bound ARF1. The complex interacts with the cargo protein CHS3. Ref.5 Ref.6 Ref.7
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Trans-Golgi network location requires interaction with CHS5 and with myristoylated GTP-bound ARF1 for the recruitment to the membranes. Ref.3 Ref.5 Ref.6
Miscellaneous	Present with 2540 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CHAPS family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane transport Inferred from genetic interaction Ref.5. Source: SGD fungal-type cell wall chitin biosynthetic process Inferred from physical interaction Ref.5. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	exomer complex Inferred from direct assay Ref.6. Source: SGD membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHS5	Q12114	6	EBI-26374,EBI-4640

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	Protein BCH2		PRO_0000203204
Regions
	Region	735 – 765	31	CHS5-binding
Amino acid modifications
	Modified residue	568	1	Phosphoserine Ref.8 Ref.9
	Modified residue	569	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P36122 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 53A4A700682287E8	FASTA	765	88,044
	10 20 30 40 50 60 MSFLWGSTKS KKGKNKKAAG SLPSGVVPQQ RVKPTRKNVP IDYPRTLEKV HGESLIFRTS 70 80 90 100 110 120 LLSELVSTGK SGIGPPDLIH CTELDKFHDE KIGEFFYITG IDASSVSMPI AFLKLIKWND 130 140 150 160 170 180 GKKLKSASLK NDDITTYCTF NIFQKLDIRL RYESEDVYQV NIVDCLNGNN EIPLSDLIWE 190 200 210 220 230 240 ETFVSCCIRS VIINSDFERK IPGLVELPFV FENRCASDYK RVIDSLCKFL PRFLECGWDS 250 260 270 280 290 300 TKSVYATILN NYLTESLLVF LSITPEFITD YAIQVLDNLM TNDPSNSRYY AIVIISIMER 310 320 330 340 350 360 SNDRDVEMIK RIHEILDLLL PVLYGLPSDE PYISDLINCI TDVLSIQARF LLNNNDYELS 370 380 390 400 410 420 LSISTLATNL SSDNFESWYL LSKGYIFSQQ YDKALLSINS MPCLAEYDIV KQAQINAFKF 430 440 450 460 470 480 YMNYYKAPLC HSREHCTMTS HELNHLMNIM HYENELELKT IIFGRTVMPN ESKYGCIEEI 490 500 510 520 530 540 WNKSCLELGP ICGPQSDNLI NFVSQQEVNT VGDMLLLKRS KETRQESWFI KQVRLLLMEL 550 560 570 580 590 600 VARIGWNALL QLRSDVFVME SKFKMIESSD KLSTELRQKR LCQRWFDAMF LDVYEDLSIS 610 620 630 640 650 660 TSSQENKATA KYSGLEWELL GLTLLRVSDL PDAVACLRTS ILARFDPISC HHLLNFYLTM 670 680 690 700 710 720 DFNDEFMRRF DVDIILDLLV KLISFRIRFY DRFQIFSLQV LRKLEGQLGS EIIKNKIINS 730 740 750 760 PYGQAGITSV IDYMLECLSK NRNEACLAYE RPLPDLPSTI KPLAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi." Trautwein M., Schindler C., Gauss R., Dengjel J., Hartmann E., Spang A. EMBO J. 25:943-954(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARF1; BCH1; BUD7; CHS3; CHS5 AND CHS6, DOMAIN.
[6]	"Exomer: a coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast." Wang C.-W., Hamamoto S., Orci L., Schekman R. J. Cell Biol. 174:973-983(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH ARF1, SUBCELLULAR LOCATION.
[7]	"Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface." Sanchatjate S., Schekman R. Mol. Biol. Cell 17:4157-4166(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH CHS3.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568 AND SER-569, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28252 Genomic DNA. Translation: CAA82099.1. BK006944 Genomic DNA. Translation: DAA09182.1.
PIR	S38099.
RefSeq	NP_012952.3. NM_001179817.3.
3D structure databases
ProteinModelPortal	P36122.
SMR	P36122. Positions 44-735.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36122. 6 interactions.
MINT	MINT-600256.
STRING	4932.YKR027W.
Proteomic databases
PaxDb	P36122.
PeptideAtlas	P36122.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR027W; YKR027W; YKR027W.
GeneID	853898.
KEGG	sce:YKR027W. sce:YKR029C.
Organism-specific databases
CYGD	YKR027w.
SGD	S000001735. BCH2.
Phylogenomic databases
eggNOG	NOG74238.
GeneTree	ENSGT00530000065203.
HOGENOM	HOG000095187.
KO	K07117.
OrthoDB	EOG41RT3M.
Gene expression databases
Genevestigator	P36122.
GermOnline	YKR027W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015374. ChAPs. [Graphical view]
Pfam	PF09295. ChAPs. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	975218.

Entry information

Entry name

BCH2_YEAST

Accession

Primary (citable) accession number: P36122
Secondary accession number(s): D6VX92

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program