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P36122

- BCH2_YEAST

UniProt

P36122 - BCH2_YEAST

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Protein

Protein BCH2

Gene

BCH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Member of the CHS5-ARF1P-binding proteins (CHAPS) which mediates export of specific cargo proteins, including chitin synthase CHS3.3 Publications

GO - Biological processi

  1. fungal-type cell wall chitin biosynthetic process Source: SGD
  2. Golgi to plasma membrane transport Source: SGD
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32003-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BCH2
Alternative name(s):
BUD7 and CHS6 homolog 2
Gene namesi
Name:BCH2
Synonyms:FMP50
Ordered Locus Names:YKR027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR027w.
SGDiS000001735. BCH2.

Subcellular locationi

Golgi apparatustrans-Golgi network membrane 3 Publications; Peripheral membrane protein 3 Publications
Note: Trans-Golgi network location requires interaction with CHS5 and with myristoylated GTP-bound ARF1 for the recruitment to the membranes.

GO - Cellular componenti

  1. exomer complex Source: SGD
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 765765Protein BCH2PRO_0000203204Add
BLAST

Proteomic databases

MaxQBiP36122.
PaxDbiP36122.
PeptideAtlasiP36122.

Expressioni

Gene expression databases

GenevestigatoriP36122.

Interactioni

Subunit structurei

Component of the CHS5/6 complex composed of the 4 CHAPS proteins BCH1, BCH2, BUD7, and CHS6 as well as at least CHS5 and GTP-bound ARF1. The complex interacts with the cargo protein CHS3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARF1P110762EBI-26374,EBI-2816
BCH1Q050294EBI-26374,EBI-27508
CHS5Q121147EBI-26374,EBI-4640
SHO1P400732EBI-26374,EBI-18140

Protein-protein interaction databases

BioGridi34160. 56 interactions.
IntActiP36122. 9 interactions.
MINTiMINT-600256.
STRINGi4932.YKR027W.

Structurei

3D structure databases

ProteinModelPortaliP36122.
SMRiP36122. Positions 44-735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni735 – 76531CHS5-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the CHAPS family.Curated

Phylogenomic databases

eggNOGiNOG74238.
GeneTreeiENSGT00530000065203.
HOGENOMiHOG000095187.
InParanoidiP36122.
OMAiHYENELE.
OrthoDBiEOG7W15DF.

Family and domain databases

InterProiIPR015374. ChAPs.
[Graphical view]
PfamiPF09295. ChAPs. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36122-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFLWGSTKS KKGKNKKAAG SLPSGVVPQQ RVKPTRKNVP IDYPRTLEKV
60 70 80 90 100
HGESLIFRTS LLSELVSTGK SGIGPPDLIH CTELDKFHDE KIGEFFYITG
110 120 130 140 150
IDASSVSMPI AFLKLIKWND GKKLKSASLK NDDITTYCTF NIFQKLDIRL
160 170 180 190 200
RYESEDVYQV NIVDCLNGNN EIPLSDLIWE ETFVSCCIRS VIINSDFERK
210 220 230 240 250
IPGLVELPFV FENRCASDYK RVIDSLCKFL PRFLECGWDS TKSVYATILN
260 270 280 290 300
NYLTESLLVF LSITPEFITD YAIQVLDNLM TNDPSNSRYY AIVIISIMER
310 320 330 340 350
SNDRDVEMIK RIHEILDLLL PVLYGLPSDE PYISDLINCI TDVLSIQARF
360 370 380 390 400
LLNNNDYELS LSISTLATNL SSDNFESWYL LSKGYIFSQQ YDKALLSINS
410 420 430 440 450
MPCLAEYDIV KQAQINAFKF YMNYYKAPLC HSREHCTMTS HELNHLMNIM
460 470 480 490 500
HYENELELKT IIFGRTVMPN ESKYGCIEEI WNKSCLELGP ICGPQSDNLI
510 520 530 540 550
NFVSQQEVNT VGDMLLLKRS KETRQESWFI KQVRLLLMEL VARIGWNALL
560 570 580 590 600
QLRSDVFVME SKFKMIESSD KLSTELRQKR LCQRWFDAMF LDVYEDLSIS
610 620 630 640 650
TSSQENKATA KYSGLEWELL GLTLLRVSDL PDAVACLRTS ILARFDPISC
660 670 680 690 700
HHLLNFYLTM DFNDEFMRRF DVDIILDLLV KLISFRIRFY DRFQIFSLQV
710 720 730 740 750
LRKLEGQLGS EIIKNKIINS PYGQAGITSV IDYMLECLSK NRNEACLAYE
760
RPLPDLPSTI KPLAD
Length:765
Mass (Da):88,044
Last modified:June 1, 1994 - v1
Checksum:i53A4A700682287E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28252 Genomic DNA. Translation: CAA82099.1.
BK006944 Genomic DNA. Translation: DAA09182.1.
PIRiS38099.
RefSeqiNP_012952.3. NM_001179817.3.

Genome annotation databases

EnsemblFungiiYKR027W; YKR027W; YKR027W.
GeneIDi853898.
KEGGisce:YKR027W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28252 Genomic DNA. Translation: CAA82099.1 .
BK006944 Genomic DNA. Translation: DAA09182.1 .
PIRi S38099.
RefSeqi NP_012952.3. NM_001179817.3.

3D structure databases

ProteinModelPortali P36122.
SMRi P36122. Positions 44-735.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34160. 56 interactions.
IntActi P36122. 9 interactions.
MINTi MINT-600256.
STRINGi 4932.YKR027W.

Proteomic databases

MaxQBi P36122.
PaxDbi P36122.
PeptideAtlasi P36122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR027W ; YKR027W ; YKR027W .
GeneIDi 853898.
KEGGi sce:YKR027W.

Organism-specific databases

CYGDi YKR027w.
SGDi S000001735. BCH2.

Phylogenomic databases

eggNOGi NOG74238.
GeneTreei ENSGT00530000065203.
HOGENOMi HOG000095187.
InParanoidi P36122.
OMAi HYENELE.
OrthoDBi EOG7W15DF.

Enzyme and pathway databases

BioCyci YEAST:G3O-32003-MONOMER.

Miscellaneous databases

NextBioi 975218.

Gene expression databases

Genevestigatori P36122.

Family and domain databases

InterProi IPR015374. ChAPs.
[Graphical view ]
Pfami PF09295. ChAPs. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi."
    Trautwein M., Schindler C., Gauss R., Dengjel J., Hartmann E., Spang A.
    EMBO J. 25:943-954(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARF1; BCH1; BUD7; CHS3; CHS5 AND CHS6, DOMAIN.
  6. "Exomer: a coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast."
    Wang C.-W., Hamamoto S., Orci L., Schekman R.
    J. Cell Biol. 174:973-983(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH ARF1, SUBCELLULAR LOCATION.
  7. "Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface."
    Sanchatjate S., Schekman R.
    Mol. Biol. Cell 17:4157-4166(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH CHS3.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBCH2_YEAST
AccessioniPrimary (citable) accession number: P36122
Secondary accession number(s): D6VX92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2540 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3