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P36122

- BCH2_YEAST

UniProt

P36122 - BCH2_YEAST

Protein

Protein BCH2

Gene

BCH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Member of the CHS5-ARF1P-binding proteins (CHAPS) which mediates export of specific cargo proteins, including chitin synthase CHS3.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. fungal-type cell wall chitin biosynthetic process Source: SGD
    2. Golgi to plasma membrane transport Source: SGD
    3. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32003-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein BCH2
    Alternative name(s):
    BUD7 and CHS6 homolog 2
    Gene namesi
    Name:BCH2
    Synonyms:FMP50
    Ordered Locus Names:YKR027W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR027w.
    SGDiS000001735. BCH2.

    Subcellular locationi

    Golgi apparatustrans-Golgi network membrane 3 Publications; Peripheral membrane protein 3 Publications
    Note: Trans-Golgi network location requires interaction with CHS5 and with myristoylated GTP-bound ARF1 for the recruitment to the membranes.

    GO - Cellular componenti

    1. exomer complex Source: SGD
    2. membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 765765Protein BCH2PRO_0000203204Add
    BLAST

    Proteomic databases

    MaxQBiP36122.
    PaxDbiP36122.
    PeptideAtlasiP36122.

    Expressioni

    Gene expression databases

    GenevestigatoriP36122.

    Interactioni

    Subunit structurei

    Component of the CHS5/6 complex composed of the 4 CHAPS proteins BCH1, BCH2, BUD7, and CHS6 as well as at least CHS5 and GTP-bound ARF1. The complex interacts with the cargo protein CHS3.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARF1P110762EBI-26374,EBI-2816
    BCH1Q050294EBI-26374,EBI-27508
    CHS5Q121147EBI-26374,EBI-4640
    SHO1P400732EBI-26374,EBI-18140

    Protein-protein interaction databases

    BioGridi34160. 55 interactions.
    IntActiP36122. 9 interactions.
    MINTiMINT-600256.
    STRINGi4932.YKR027W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36122.
    SMRiP36122. Positions 44-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni735 – 76531CHS5-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CHAPS family.Curated

    Phylogenomic databases

    eggNOGiNOG74238.
    GeneTreeiENSGT00530000065203.
    HOGENOMiHOG000095187.
    OMAiHYENELE.
    OrthoDBiEOG7W15DF.

    Family and domain databases

    InterProiIPR015374. ChAPs.
    [Graphical view]
    PfamiPF09295. ChAPs. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36122-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFLWGSTKS KKGKNKKAAG SLPSGVVPQQ RVKPTRKNVP IDYPRTLEKV    50
    HGESLIFRTS LLSELVSTGK SGIGPPDLIH CTELDKFHDE KIGEFFYITG 100
    IDASSVSMPI AFLKLIKWND GKKLKSASLK NDDITTYCTF NIFQKLDIRL 150
    RYESEDVYQV NIVDCLNGNN EIPLSDLIWE ETFVSCCIRS VIINSDFERK 200
    IPGLVELPFV FENRCASDYK RVIDSLCKFL PRFLECGWDS TKSVYATILN 250
    NYLTESLLVF LSITPEFITD YAIQVLDNLM TNDPSNSRYY AIVIISIMER 300
    SNDRDVEMIK RIHEILDLLL PVLYGLPSDE PYISDLINCI TDVLSIQARF 350
    LLNNNDYELS LSISTLATNL SSDNFESWYL LSKGYIFSQQ YDKALLSINS 400
    MPCLAEYDIV KQAQINAFKF YMNYYKAPLC HSREHCTMTS HELNHLMNIM 450
    HYENELELKT IIFGRTVMPN ESKYGCIEEI WNKSCLELGP ICGPQSDNLI 500
    NFVSQQEVNT VGDMLLLKRS KETRQESWFI KQVRLLLMEL VARIGWNALL 550
    QLRSDVFVME SKFKMIESSD KLSTELRQKR LCQRWFDAMF LDVYEDLSIS 600
    TSSQENKATA KYSGLEWELL GLTLLRVSDL PDAVACLRTS ILARFDPISC 650
    HHLLNFYLTM DFNDEFMRRF DVDIILDLLV KLISFRIRFY DRFQIFSLQV 700
    LRKLEGQLGS EIIKNKIINS PYGQAGITSV IDYMLECLSK NRNEACLAYE 750
    RPLPDLPSTI KPLAD 765
    Length:765
    Mass (Da):88,044
    Last modified:June 1, 1994 - v1
    Checksum:i53A4A700682287E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28252 Genomic DNA. Translation: CAA82099.1.
    BK006944 Genomic DNA. Translation: DAA09182.1.
    PIRiS38099.
    RefSeqiNP_012952.3. NM_001179817.3.

    Genome annotation databases

    EnsemblFungiiYKR027W; YKR027W; YKR027W.
    GeneIDi853898.
    KEGGisce:YKR027W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28252 Genomic DNA. Translation: CAA82099.1 .
    BK006944 Genomic DNA. Translation: DAA09182.1 .
    PIRi S38099.
    RefSeqi NP_012952.3. NM_001179817.3.

    3D structure databases

    ProteinModelPortali P36122.
    SMRi P36122. Positions 44-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34160. 55 interactions.
    IntActi P36122. 9 interactions.
    MINTi MINT-600256.
    STRINGi 4932.YKR027W.

    Proteomic databases

    MaxQBi P36122.
    PaxDbi P36122.
    PeptideAtlasi P36122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR027W ; YKR027W ; YKR027W .
    GeneIDi 853898.
    KEGGi sce:YKR027W.

    Organism-specific databases

    CYGDi YKR027w.
    SGDi S000001735. BCH2.

    Phylogenomic databases

    eggNOGi NOG74238.
    GeneTreei ENSGT00530000065203.
    HOGENOMi HOG000095187.
    OMAi HYENELE.
    OrthoDBi EOG7W15DF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32003-MONOMER.

    Miscellaneous databases

    NextBioi 975218.

    Gene expression databases

    Genevestigatori P36122.

    Family and domain databases

    InterProi IPR015374. ChAPs.
    [Graphical view ]
    Pfami PF09295. ChAPs. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi."
      Trautwein M., Schindler C., Gauss R., Dengjel J., Hartmann E., Spang A.
      EMBO J. 25:943-954(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARF1; BCH1; BUD7; CHS3; CHS5 AND CHS6, DOMAIN.
    6. "Exomer: a coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast."
      Wang C.-W., Hamamoto S., Orci L., Schekman R.
      J. Cell Biol. 174:973-983(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH ARF1, SUBCELLULAR LOCATION.
    7. "Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface."
      Sanchatjate S., Schekman R.
      Mol. Biol. Cell 17:4157-4166(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE CHS5/6 COMPLEX, INTERACTION WITH CHS3.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBCH2_YEAST
    AccessioniPrimary (citable) accession number: P36122
    Secondary accession number(s): D6VX92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2540 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3