P36121 (RPC5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase III subunit RPC5
Short name=RNA polymerase III subunit C5

Alternative name(s):
DNA-directed RNA polymerase III 37 kDa polypeptide
RNA polymerase III subunit C37

Gene names

Name:	RPC37
Ordered Locus Names:	YKR025W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. The RPC53/RPC4-RPC37/RPC5 subcomplex is required for terminator recognition and reinitiation. Ref.8
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Interacts with RPC53/RPC4. RPC53/RPC4, RPC37/RPC5 and RPC11/RPC10 probably form a Pol III subcomplex. Ref.5
Subcellular location	Nucleus Ref.6.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA transcription from RNA polymerase III promoter Inferred from direct assay PubMed 3905793. Source: SGD
Cellular_component	DNA-directed RNA polymerase III complex Inferred from direct assay PubMed 10611227PubMed 3905793. Source: SGD
Molecular_function	DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	DNA-directed RNA polymerase III subunit RPC5		PRO_0000203203
Amino acid modifications
	Modified residue	18	1	Phosphothreonine Ref.10
	Modified residue	52	1	Phosphoserine Ref.10
	Modified residue	61	1	Phosphothreonine Ref.9 Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P36121 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 9E948BDDECE9D15E	FASTA	282	32,142
	10 20 30 40 50 60 MSIDNKLFVT EEDEEDRTQD RADVEDESND IDMIADENGT NSAIANEQEE KSEEVKAEDD 70 80 90 100 110 120 TGEEEEDDPV IEEFPLKISG EEESLHVFQY ANRPRLVGRK PAEHPFISAA RYKPKSHLWE 130 140 150 160 170 180 IDIPLDEQAF YNKDKAESEW NGVNVQTLKG VGVENNGQYA AFVKDMQVYL VPIERVAQLK 190 200 210 220 230 240 PFFKYIDDAN VTRKQEDARR NPNPSSQRAQ VVTMSVKSVN DPSQNRLTGS LLAHKVADEE 250 260 270 280 ANIELTWAEG TFEQFKDTIV KEAEDKTLVA LEKQEDYIDN LV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation." Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A. Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW ON THE RNA POL III COMPLEX.
[5]	"A protein-protein interaction map of yeast RNA polymerase III." Flores A., Briand J.-F., Gadal O., Andrau J.-C., Rubbi L., Van Mullem V., Boschiero C., Goussot M., Marck C., Carles C., Thuriaux P., Sentenac A., Werner M. Proc. Natl. Acad. Sci. U.S.A. 96:7815-7820(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, INTERACTION WITH RPC4.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A subcomplex of RNA polymerase III subunits involved in transcription termination and reinitiation." Landrieux E., Alic N., Ducrot C., Acker J., Riva M., Carles C. EMBO J. 25:118-128(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RPC53-RPC37 SUBCOMPLEX.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-61, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18; SER-52 AND THR-61, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28250 Genomic DNA. Translation: CAA82097.1. AY692886 Genomic DNA. Translation: AAT92905.1. BK006944 Genomic DNA. Translation: DAA09180.1.
PIR	S38094.
RefSeq	NP_012950.3. NM_001179815.3.
3D structure databases
ProteinModelPortal	P36121.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-761N.
IntAct	P36121. 9 interactions.
MINT	MINT-399243.
STRING	4932.YKR025W.
Proteomic databases
PaxDb	P36121.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR025W; YKR025W; YKR025W.
GeneID	853895.
KEGG	sce:YKR025W. sce:YKR027W.
Organism-specific databases
CYGD	YKR025w.
SGD	S000001733. RPC37.
Phylogenomic databases
eggNOG	NOG276044.
HOGENOM	HOG000074717.
KO	K14721.
OMA	INDASTG.
OrthoDB	EOG40CMSK.
Gene expression databases
Genevestigator	P36121.
GermOnline	YKR025W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006886. RNA_pol_III_Rpc5. [Graphical view]
Pfam	PF04801. Sin_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975210.

Entry information

Entry name

RPC5_YEAST

Accession

Primary (citable) accession number: P36121
Secondary accession number(s): D6VX90

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program