P36118 (NTR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor NTR2

Alternative name(s):
Nineteen complex-related protein 2
Short name=NTC-related protein 2

Gene names

Name:	NTR2
Ordered Locus Names:	YKR022C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing and spliceosome disassembly. Promotes release of excised lariat intron from the spliceosome by acting as a receptor for PRP43. This targeting of PRP43 leads to disassembly of the spliceosome with the separation of the U2, U5, U6 snRNPs and the NTC complex. Ref.5 Ref.6
Subunit structure	Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43. Interacts with CLF1, NTR1 and PRP43. Ref.5 Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.3.
Miscellaneous	Present with 892 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	spliceosomal complex disassembly Inferred from direct assay Ref.5. Source: SGD
Cellular_component	U2-type post-mRNA release spliceosomal complex Inferred from direct assay Ref.5. Source: SGD endoplasmic reticulum Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPP382	Q06411	11	EBI-26362,EBI-576

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Pre-mRNA-splicing factor NTR2		PRO_0000203201
Amino acid modifications
	Modified residue	40	1	Phosphoserine Ref.7
	Modified residue	153	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P36118 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D7A601A46839244C	FASTA	322	36,647
	10 20 30 40 50 60 MAIKKRNKIR LPSGSPEEVG IDGSAHKPMQ QIKPLVSNDS EDDDNDICVL QPIKFKKVPK 70 80 90 100 110 120 RDITFDGEQA IKEDNSHYED LYHSKKNTNA STRNKDDLLI LNMEDLMEGN HHLLSDSSEA 130 140 150 160 170 180 GSSSEGEHIS SIPTRGEIAK LKAQKSLSRR KISESDVTTE RDYVKLLDSE DKREIMETIR 190 200 210 220 230 240 LNGGLKRNNE KEITNFSDDE MQGFQDEMLA LTDNQIAIQK DSKRKIIEKA INEVPYRTNE 250 260 270 280 290 300 EWETQLLSKG NINKSNEKII TPLPVLFPDD DESGNSIERI NEMVSKICLQ RKKVEMRLQA 310 320 LEKTKIDLEK SKASLINKLI GN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2." Tsai R.-T., Fu R.-H., Yeh F.-L., Tseng C.-K., Lin Y.-C., Huang Y.-H., Cheng S.-C. Genes Dev. 19:2991-3003(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE NTC-RELATED COMPLEX, INTERACTION WITH CLF1; NTR1 AND PRP43.
[6]	"Yeast ntr1/spp382 mediates prp43 function in postspliceosomes." Boon K.-L., Auchynnikava T., Edwalds-Gilbert G., Barrass J.D., Droop A.P., Dez C., Beggs J.D. Mol. Cell. Biol. 26:6016-6023(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NTR1 AND PRP43.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40 AND SER-153, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28247 Genomic DNA. Translation: CAA82094.1. BK006944 Genomic DNA. Translation: DAA09177.1.
PIR	S38091.
RefSeq	NP_012947.1. NM_001179812.1.
3D structure databases
ProteinModelPortal	P36118.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1924N.
IntAct	P36118. 19 interactions.
MINT	MINT-403676.
STRING	4932.YKR022C.
Proteomic databases
PaxDb	P36118.
PeptideAtlas	P36118.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR022C; YKR022C; YKR022C.
GeneID	853892.
KEGG	sce:YKR022C.
Organism-specific databases
CYGD	YKR022c.
SGD	S000001730. NTR2.
Phylogenomic databases
eggNOG	NOG244380.
OrthoDB	EOG4WQ4CJ.
Gene expression databases
Genevestigator	P36118.
GermOnline	YKR022C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	975201.

Entry information

Entry name

NTR2_YEAST

Accession

Primary (citable) accession number: P36118
Secondary accession number(s): D6VX87

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program