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P36118 (NTR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor NTR2
Alternative name(s):
Nineteen complex-related protein 2
Short name=NTC-related protein 2
Gene names
Name:NTR2
Ordered Locus Names:YKR022C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pre-mRNA splicing and spliceosome disassembly. Promotes release of excised lariat intron from the spliceosome by acting as a receptor for PRP43. This targeting of PRP43 leads to disassembly of the spliceosome with the separation of the U2, U5, U6 snRNPs and the NTC complex. Ref.5 Ref.6

Subunit structure

Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43. Interacts with CLF1, NTR1 and PRP43. Ref.5 Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.3.

Miscellaneous

Present with 892 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SPP382Q0641111EBI-26362,EBI-576

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Pre-mRNA-splicing factor NTR2
PRO_0000203201

Amino acid modifications

Modified residue401Phosphoserine Ref.7 Ref.8
Modified residue1531Phosphoserine Ref.7
Modified residue1971Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P36118 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D7A601A46839244C

FASTA32236,647
        10         20         30         40         50         60 
MAIKKRNKIR LPSGSPEEVG IDGSAHKPMQ QIKPLVSNDS EDDDNDICVL QPIKFKKVPK 

        70         80         90        100        110        120 
RDITFDGEQA IKEDNSHYED LYHSKKNTNA STRNKDDLLI LNMEDLMEGN HHLLSDSSEA 

       130        140        150        160        170        180 
GSSSEGEHIS SIPTRGEIAK LKAQKSLSRR KISESDVTTE RDYVKLLDSE DKREIMETIR 

       190        200        210        220        230        240 
LNGGLKRNNE KEITNFSDDE MQGFQDEMLA LTDNQIAIQK DSKRKIIEKA INEVPYRTNE 

       250        260        270        280        290        300 
EWETQLLSKG NINKSNEKII TPLPVLFPDD DESGNSIERI NEMVSKICLQ RKKVEMRLQA 

       310        320 
LEKTKIDLEK SKASLINKLI GN 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2."
Tsai R.-T., Fu R.-H., Yeh F.-L., Tseng C.-K., Lin Y.-C., Huang Y.-H., Cheng S.-C.
Genes Dev. 19:2991-3003(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE NTC-RELATED COMPLEX, INTERACTION WITH CLF1; NTR1 AND PRP43.
[6]"Yeast ntr1/spp382 mediates prp43 function in postspliceosomes."
Boon K.-L., Auchynnikava T., Edwalds-Gilbert G., Barrass J.D., Droop A.P., Dez C., Beggs J.D.
Mol. Cell. Biol. 26:6016-6023(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NTR1 AND PRP43.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40 AND SER-153, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40 AND SER-197, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28247 Genomic DNA. Translation: CAA82094.1.
BK006944 Genomic DNA. Translation: DAA09177.1.
PIRS38091.
RefSeqNP_012947.1. NM_001179812.1.

3D structure databases

ProteinModelPortalP36118.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34154. 61 interactions.
DIPDIP-1924N.
IntActP36118. 20 interactions.
MINTMINT-403676.
STRING4932.YKR022C.

Proteomic databases

PaxDbP36118.
PeptideAtlasP36118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR022C; YKR022C; YKR022C.
GeneID853892.
KEGGsce:YKR022C.

Organism-specific databases

CYGDYKR022c.
SGDS000001730. NTR2.

Phylogenomic databases

eggNOGNOG244380.
OrthoDBEOG7CG796.

Enzyme and pathway databases

BioCycYEAST:G3O-31998-MONOMER.

Gene expression databases

GenevestigatorP36118.

Family and domain databases

InterProIPR028211. Ntr2.
[Graphical view]
PfamPF15458. NTR2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975201.

Entry information

Entry nameNTR2_YEAST
AccessionPrimary (citable) accession number: P36118
Secondary accession number(s): D6VX87
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD