Reviewed, UniProtKB/Swiss-Prot P36115 (IRS4_YEAST)

Names and origin

Protein names

Recommended name:
    Increased rDNA silencing protein 4

Gene names

Name:	IRS4
Ordered Locus Names:	YKR019C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	With TAX4, acts as a positive regulator of INP51 activity and phosphatidylinositol 4,5-bisphosphate turnover. Negatively regulates signaling through the cell integrity pathway, including the MAP kinase SLT2. Seems also to be involved in rDNA silencing. Ref.3 Ref.4
Subunit structure	Interacts with INP51. Ref.4
Sequence similarities	Belongs to the IRS4 family. Contains 1 EH domain.

Ontologies

Keywords
Biological process	Lipid metabolism
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from genetic interaction. Source: SGD cell wall organization Ref.4 Inferred from genetic interaction. Source: SGD cellular response to starvation Inferred from genetic interaction. Source: SGD chromatin silencing at rDNA Ref.3 Inferred from mutant phenotype. Source: SGD inositol lipid-mediated signaling Ref.4 Inferred from genetic interaction. Source: SGD lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD pre-autophagosomal structure Inferred from direct assay. Source: SGD
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
INP51	P40559	1	EBI-27090,EBI-24915

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Increased rDNA silencing protein 4		PRO_0000203199
Regions
	Domain	460 – 571	112	EH
	Compositional bias	122 – 282	161	Ser-rich
Amino acid modifications
	Modified residue	180	1	Phosphoserine Ref.6
	Modified residue	614	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P36115-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 242EBE45978068FD	FASTA	615	68,880
	10 20 30 40 50 60 MRLSFGKQRY HGGTTVTLTE QGASDSLRAA QAIFQNHSNE VSSPCPPVTV SRNPQTRLSE 70 80 90 100 110 120 PSLQKSGRKQ EQKKARIRTK QVPKIKTTAP NDVELSKKHR SSPAGKDNVS STAQMAAALA 130 140 150 160 170 180 HSQSKLSSDN NSSHSSALDT LKVLETPNLN GLLGIHSRSS SRNGSNESLT PGQRTPDNRS 190 200 210 220 230 240 QENLLTSFSS GRRLSSSSME PATNKDSNKA LPKRRPSPPL QSSLVGSGQL HENENLSSIS 250 260 270 280 290 300 IDSRHSLNPD TSDVISNRSQ TSLSQTINQL SLCESEPSIA SSNTTTTTSN QGSGLPNLVP 310 320 330 340 350 360 NYSSDMRKKK LVNKFKRKVF GSKPKHLSSQ YEMDASSEEL GQHEQQPSMR FKTTLRKTSV 370 380 390 400 410 420 STNAENDHAS SLHEGNLRYK YNPSNDTYDV YDDTDSDSES DQNQDALTKP RKRDRIKRKI 430 440 450 460 470 480 RNSANKTAHH RPIHRTRDRK FNEDKPWKSH TDITFVTDNE RKRYESMWVS NRHRHLNLLS 490 500 510 520 530 540 WWPSITGDSG AINTLPEDGL ILGIIVRDIW KRSNLPNSLL AEIYTKVDTR KDGTLDRKSF 550 560 570 580 590 600 IVGMWLVDQC LYGRKLPNVV EQCVWDSVDR YASTTVVPVS TLKAMAKQKR KQMKEEIKNI 610 KKENRVVLVD HNSSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Recognition specificity of individual EH domains of mammals and yeast." Paoluzi S., Castagnoli L., Lauro I., Salcini A.E., Coda L., Fre' S., Confalonieri S., Pelicci P.G., Di Fiore P.P., Cesareni G. EMBO J. 17:6541-6550(1998) [PubMed: 9822599] [Abstract] Cited for: DOMAIN.
[3]	"A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors." Smith J.S., Caputo E., Boeke J.D. Mol. Cell. Biol. 19:3184-3197(1999) [PubMed: 10082585] [Abstract] Cited for: FUNCTION.
[4]	"Negative regulation of phosphatidylinositol 4,5-bisphosphate levels by the INP51-associated proteins TAX4 and IRS4." Morales-Johansson H., Jenoe P., Cooke F.T., Hall M.N. J. Biol. Chem. 279:39604-39610(2004) [PubMed: 15265867] [Abstract] Cited for: FUNCTION, INTERACTION WITH INP51.
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-614, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28244 Genomic DNA. Translation: CAA82091.1.
PIR	S38088.
RefSeq	NP_012944.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4852N.
IntAct	P36115. 2 interactions.
STRING	P36115.
Genome annotation databases
Ensembl	YKR019C; YKR019C; YKR019C; Saccharomyces cerevisiae. [Genome view]
GeneID	853889.
KEGG	sce:YKR019C.
NMPDR	fig|4932.3.peg.3930.
Organism-specific databases
CYGD	YKR019c.
SGD	S000001727. IRS4.
Phylogenomic databases
OrthoDB	EOG9H47MJ
Gene expression databases
ArrayExpress	P36115.
Genevestigator	P36115.
GermOnline	YKR019C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR000261. EPS15_homology. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
SMART	SM00027. EH. 1 hit. [Graphical view]
PROSITE	PS50031. EH. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975192.

Entry information

Entry name

IRS4_YEAST

Accession

Primary (citable) accession number: P36115

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)