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Protein

DOA4-independent degradation protein 4

Gene

DID4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Can directly stimulate VPS4 ATPase activity. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III.3 Publications

GO - Biological processi

  • ATP export Source: SGD
  • intralumenal vesicle formation Source: SGD
  • late endosome to vacuole transport Source: SGD
  • protein retention in Golgi apparatus Source: SGD
  • protein transport Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31813-MONOMER
ReactomeiR-SCE-1632852 Macroautophagy
R-SCE-432720 Lysosome Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
DOA4-independent degradation protein 4
Alternative name(s):
ESCRT-III complex subunit VPS2
Vacuolar protein-sorting-associated protein 2
Vacuolar protein-targeting protein 14
Gene namesi
Name:DID4
Synonyms:CHM2, GRD7, REN1, VPL2, VPS2, VPT14
Ordered Locus Names:YKL002W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL002W
SGDiS000001485 DID4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi221L → D: Abolishes interaction with VPS4. 1 Publication1
Mutagenesisi224R → D: Abolishes interaction with VPS4. Impairs sorting. 1 Publication1
Mutagenesisi225L → D: Abolishes interaction with VPS4. 1 Publication1
Mutagenesisi228 – 229LK → DD: Greatly impairs sorting. 2
Mutagenesisi228L → D: Abolishes interaction with VPS4. 1 Publication1
Mutagenesisi229K → D: Abolishes interaction with VPS4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114771 – 232DOA4-independent degradation protein 4Add BLAST232

Proteomic databases

MaxQBiP36108
PaxDbiP36108
PRIDEiP36108

PTM databases

iPTMnetiP36108

Interactioni

Subunit structurei

Core component of the ESCRT-III complex (endosomal sorting required for transport complex III). ESCRT-III appears to be sequentially assembled as a flat lattice on the endosome membrane and forms a transient 450 kDa complex that contains DID4, oligomerized SNF7, VPS20 and VPS24. SNF7 oligomerization into a membrane-associated filament is nucleated by association of SNF7 with VPS20; the process is terminated through association of VPS24, possibly by capping the SNF7 filament. VPS24 subsequently associates with DID4/VPS2.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34131, 224 interactors
ComplexPortaliCPX-1624 ESCRT-III complex
DIPiDIP-1916N
IntActiP36108, 14 interactors
MINTiP36108
STRINGi4932.YKL002W

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi188 – 191Combined sources4
Helixi197 – 200Combined sources4
Beta strandi201 – 204Combined sources4
Helixi219 – 229Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6XX-ray1.98B183-232[»]
6AP1electron microscopy3.20G165-172[»]
6BMFelectron microscopy3.20G165-172[»]
ProteinModelPortaliP36108
SMRiP36108
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36108

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni183 – 232Interaction with VPS41 PublicationAdd BLAST50

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 97Sequence analysisAdd BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi219 – 229MIT-interacting motifAdd BLAST11

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074737
HOGENOMiHOG000177218
InParanoidiP36108
KOiK12191
OMAiNMNRQLN
OrthoDBiEOG092C4XGL

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit

Sequencei

Sequence statusi: Complete.

P36108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFEWVFGK NVTPQERLKK NQRALERTQR ELEREKRKLE LQDKKLVSEI
60 70 80 90 100
KKSAKNGQVA AAKVQAKDLV RTRNYIQKFD NMKAQLQAIS LRIQAVRSSD
110 120 130 140 150
QMTRSMSEAT GLLAGMNRTM NLPQLQRISM EFEKQSDLMG QRQEFMDEAI
160 170 180 190 200
DNVMGDEVDE DEEADEIVNK VLDEIGVDLN SQLQSTPQNL VSNAPIAETA
210 220 230
MGIPEPIGAG SEFHGNPDDD LQARLNTLKK QT
Length:232
Mass (Da):26,291
Last modified:October 17, 2006 - v2
Checksum:i200D07DB4C45DCBC
GO

Sequence cautioni

The sequence CAA81834 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28002 Genomic DNA Translation: CAA81834.1 Sequence problems.
BK006944 Genomic DNA Translation: DAA09155.1
PIRiS37812
RefSeqiNP_012924.2, NM_001179568.1

Genome annotation databases

EnsemblFungiiYKL002W; YKL002W; YKL002W
GeneIDi853868
KEGGisce:YKL002W

Similar proteinsi

Entry informationi

Entry nameiDID4_YEAST
AccessioniPrimary (citable) accession number: P36108
Secondary accession number(s): D6VXT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 17, 2006
Last modified: June 20, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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