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Protein

60S ribosomal protein L14-A

Gene

RPL14A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. RNA binding Source: SGD
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-31816-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L14-A
Gene namesi
Name:RPL14A
Ordered Locus Names:YKL006W
ORF Names:YKL153
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL006w.
SGDiS000001489. RPL14A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 13813760S ribosomal protein L14-APRO_0000132043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36105.
PeptideAtlasiP36105.

Expressioni

Gene expression databases

GenevestigatoriP36105.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34127. 168 interactions.
IntActiP36105. 18 interactions.
MINTiMINT-4492283.
STRINGi4932.YKL006W.

Structurei

Secondary structure

1
138
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 235Combined sources
Beta strandi25 – 284Combined sources
Beta strandi32 – 387Combined sources
Beta strandi40 – 489Combined sources
Helixi49 – 513Combined sources
Beta strandi53 – 597Combined sources
Helixi60 – 623Combined sources
Beta strandi63 – 697Combined sources
Helixi78 – 8811Combined sources
Helixi90 – 956Combined sources
Helixi98 – 11114Combined sources
Helixi114 – 13421Combined sources
Turni135 – 1373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW8electron microscopy6.10N1-138[»]
1VWUelectron microscopy6.10N1-138[»]
1VXVelectron microscopy6.20N1-138[»]
1VXYelectron microscopy6.30N1-138[»]
3IZSelectron microscopy-N1-138[»]
3J65electron microscopy8.70M1-138[»]
3O58X-ray4.00N1-138[»]
3O5HX-ray4.00N1-138[»]
3U5EX-ray3.00M1-138[»]
3U5IX-ray3.00M1-138[»]
4B6Aelectron microscopy8.10M1-138[»]
4UJGX-ray3.20O2-138[»]
4UJJX-ray3.20E2-138[»]
4UJLX-ray3.00O2-138[»]
4UJOX-ray3.00E2-138[»]
4UJQX-ray2.80O2-138[»]
4UJTX-ray2.80E2-138[»]
4UJVX-ray3.10O2-138[»]
4UJYX-ray3.10D2-138[»]
4UK0X-ray3.00O2-138[»]
4UK3X-ray3.00E2-138[»]
4UK5X-ray3.20O2-138[»]
4UK8X-ray3.20E2-138[»]
4UKAX-ray2.90O2-138[»]
4UKEX-ray2.90E2-138[»]
4UKGX-ray3.45O2-138[»]
4UKJX-ray3.45D2-138[»]
4UKLX-ray3.20O2-138[»]
4UKOX-ray3.20D2-138[»]
4UKQX-ray3.30O2-138[»]
4UKTX-ray3.30E2-138[»]
4UKVX-ray3.10O2-138[»]
4UKYX-ray3.10E2-138[»]
4UL0X-ray3.60O2-138[»]
4UL3X-ray3.60E2-138[»]
4UL5X-ray3.00O2-138[»]
4UL8X-ray3.00E2-138[»]
4ULAX-ray3.00O2-138[»]
4ULDX-ray3.00E2-138[»]
4ULFX-ray3.20O2-138[»]
4ULIX-ray3.20E2-138[»]
4ULKX-ray3.10O2-138[»]
4ULOX-ray3.10E2-138[»]
4ULQX-ray3.20O2-138[»]
4ULTX-ray3.20D2-138[»]
4V7Felectron microscopy8.70M1-138[»]
4V8Telectron microscopy8.10M1-138[»]
ProteinModelPortaliP36105.
SMRiP36105. Positions 2-138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L14e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007888.
HOGENOMiHOG000201693.
InParanoidiP36105.
KOiK02875.
OMAiVKAANWR.
OrthoDBiEOG7GFBHZ.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR014722. Rib_L2_dom2.
IPR002784. Ribosomal_L14.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF01929. Ribosomal_L14e. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36105-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK
60 70 80 90 100
AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAAV CEKWAASSWA
110 120 130
KKIAQRERRA ALTDFERFQV MVLRKQKRYT VKKALAKA
Length:138
Mass (Da):15,167
Last modified:June 1, 1994 - v1
Checksum:iF8F2A346ABB395BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61398 Genomic DNA. No translation available.
Z28006 Genomic DNA. Translation: CAA81839.1.
Z28005 Genomic DNA. Translation: CAA81838.1.
S59773 Genomic DNA. Translation: AAC60550.1.
BK006944 Genomic DNA. Translation: DAA09151.1.
PIRiS30133.
RefSeqiNP_012920.1. NM_001179572.1.

Genome annotation databases

EnsemblFungiiYKL006W; YKL006W; YKL006W.
GeneIDi853864.
KEGGisce:YKL006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61398 Genomic DNA. No translation available.
Z28006 Genomic DNA. Translation: CAA81839.1.
Z28005 Genomic DNA. Translation: CAA81838.1.
S59773 Genomic DNA. Translation: AAC60550.1.
BK006944 Genomic DNA. Translation: DAA09151.1.
PIRiS30133.
RefSeqiNP_012920.1. NM_001179572.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW8electron microscopy6.10N1-138[»]
1VWUelectron microscopy6.10N1-138[»]
1VXVelectron microscopy6.20N1-138[»]
1VXYelectron microscopy6.30N1-138[»]
3IZSelectron microscopy-N1-138[»]
3J65electron microscopy8.70M1-138[»]
3O58X-ray4.00N1-138[»]
3O5HX-ray4.00N1-138[»]
3U5EX-ray3.00M1-138[»]
3U5IX-ray3.00M1-138[»]
4B6Aelectron microscopy8.10M1-138[»]
4UJGX-ray3.20O2-138[»]
4UJJX-ray3.20E2-138[»]
4UJLX-ray3.00O2-138[»]
4UJOX-ray3.00E2-138[»]
4UJQX-ray2.80O2-138[»]
4UJTX-ray2.80E2-138[»]
4UJVX-ray3.10O2-138[»]
4UJYX-ray3.10D2-138[»]
4UK0X-ray3.00O2-138[»]
4UK3X-ray3.00E2-138[»]
4UK5X-ray3.20O2-138[»]
4UK8X-ray3.20E2-138[»]
4UKAX-ray2.90O2-138[»]
4UKEX-ray2.90E2-138[»]
4UKGX-ray3.45O2-138[»]
4UKJX-ray3.45D2-138[»]
4UKLX-ray3.20O2-138[»]
4UKOX-ray3.20D2-138[»]
4UKQX-ray3.30O2-138[»]
4UKTX-ray3.30E2-138[»]
4UKVX-ray3.10O2-138[»]
4UKYX-ray3.10E2-138[»]
4UL0X-ray3.60O2-138[»]
4UL3X-ray3.60E2-138[»]
4UL5X-ray3.00O2-138[»]
4UL8X-ray3.00E2-138[»]
4ULAX-ray3.00O2-138[»]
4ULDX-ray3.00E2-138[»]
4ULFX-ray3.20O2-138[»]
4ULIX-ray3.20E2-138[»]
4ULKX-ray3.10O2-138[»]
4ULOX-ray3.10E2-138[»]
4ULQX-ray3.20O2-138[»]
4ULTX-ray3.20D2-138[»]
4V7Felectron microscopy8.70M1-138[»]
4V8Telectron microscopy8.10M1-138[»]
ProteinModelPortaliP36105.
SMRiP36105. Positions 2-138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34127. 168 interactions.
IntActiP36105. 18 interactions.
MINTiMINT-4492283.
STRINGi4932.YKL006W.

Proteomic databases

MaxQBiP36105.
PeptideAtlasiP36105.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL006W; YKL006W; YKL006W.
GeneIDi853864.
KEGGisce:YKL006W.

Organism-specific databases

CYGDiYKL006w.
SGDiS000001489. RPL14A.

Phylogenomic databases

GeneTreeiENSGT00390000007888.
HOGENOMiHOG000201693.
InParanoidiP36105.
KOiK02875.
OMAiVKAANWR.
OrthoDBiEOG7GFBHZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31816-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

NextBioi975121.

Gene expression databases

GenevestigatoriP36105.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR014722. Rib_L2_dom2.
IPR002784. Ribosomal_L14.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF01929. Ribosomal_L14e. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene."
    Boyer J., Pascolo S., Richard G.-F., Dujon B.
    Yeast 9:279-287(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  9. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL14A_YEAST
AccessioniPrimary (citable) accession number: P36105
Secondary accession number(s): D6VXT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 4, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 45300 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L14 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.