P36105 (RL14A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L14-A

Gene names

Name:	RPL14A
Ordered Locus Names:	YKL006W
ORF Names:	YKL153

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 45300 molecules/cell in log phase SD medium. There are 2 genes for L14 in yeast.
Sequence similarities	Belongs to the ribosomal protein L14e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.4. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.4. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 6337137. Source: SGD structural constituent of ribosome Traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 138	137	60S ribosomal protein L14-A		PRO_0000132043
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P36105 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: F8F2A346ABB395BC	FASTA	138	15,167
	10 20 30 40 50 60 MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL 70 80 90 100 110 120 GQVVLTPLTF ALPRGARTAT VSKKWAAAAV CEKWAASSWA KKIAQRERRA ALTDFERFQV 130 MVLRKQKRYT VKKALAKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene." Boyer J., Pascolo S., Richard G.-F., Dujon B. Yeast 9:279-287(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X61398 Genomic DNA. No translation available.
Z28006 Genomic DNA. Translation: CAA81839.1.
Z28005 Genomic DNA. Translation: CAA81838.1.
S59773 Genomic DNA. Translation: AAC60550.1.
BK006944 Genomic DNA. Translation: DAA09151.1.

PIR

S30133.

RefSeq

NP_012920.1. NM_001179572.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZS	electron microscopy	-	N	1-138	[»]
3O58	X-ray	4.00	N	1-138	[»]
3O5H	X-ray	4.00	N	1-138	[»]
3U5E	X-ray	3.00	M	1-138	[»]
3U5I	X-ray	3.00	M	1-138	[»]
4B6A	electron microscopy	8.10	M	1-138	[»]

ProteinModelPortal

P36105.

SMR

P36105. Positions 2-138.

ModBase

Search...

Protein-protein interaction databases

IntAct

P36105. 15 interactions.

MINT

MINT-4492283.

STRING

4932.YKL006W.

Proteomic databases

PeptideAtlas

P36105.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL006W; YKL006W; YKL006W.

GeneID

853864.

KEGG

sce:YKL006W.

Organism-specific databases

CYGD

YKL006w.

SGD

S000001489. RPL14A.

Phylogenomic databases

GeneTree

ENSGT00390000007888.

HOGENOM

HOG000201693.

KO

K02875.

OMA

VQKRRAN.

OrthoDB

EOG4VT96T.

Enzyme and pathway databases

BioCyc

YEAST:G3O-31816-MONOMER.

Gene expression databases

Genevestigator

P36105.

GermOnline

YKL006W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.30.30.30. 1 hit.

InterPro

IPR005824. KOW.
IPR014722. Rib_L2_dom2.
IPR002784. Ribosomal_L14.
[Graphical view]

Pfam

PF00467. KOW. 1 hit.
PF01929. Ribosomal_L14e. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975121.

Entry information

Entry name

RL14A_YEAST

Accession

Primary (citable) accession number: P36105
Secondary accession number(s): D6VXT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program