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Protein

60S ribosomal protein L14-A

Gene

RPL14A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 45300 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL14 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: SGD
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • macroautophagy Source: SGD
  • ribosomal large subunit biogenesis Source: GO_Central
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-31816-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L14-A1 Publication
Alternative name(s):
Large ribosomal subunit protein eL14-A1 Publication
Gene namesi
Name:RPL14A1 Publication
Ordered Locus Names:YKL006W
ORF Names:YKL153
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL006W.
SGDiS000001489. RPL14A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001320432 – 13860S ribosomal protein L14-AAdd BLAST137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36105.
PRIDEiP36105.

PTM databases

iPTMnetiP36105.

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi34127. 499 interactors.
DIPiDIP-8081N.
IntActiP36105. 18 interactors.
MINTiMINT-4492283.
STRINGi4932.YKL006W.

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 23Combined sources5
Beta strandi25 – 28Combined sources4
Beta strandi32 – 38Combined sources7
Beta strandi40 – 48Combined sources9
Helixi49 – 51Combined sources3
Beta strandi53 – 59Combined sources7
Helixi60 – 62Combined sources3
Beta strandi63 – 69Combined sources7
Helixi78 – 88Combined sources11
Helixi90 – 95Combined sources6
Helixi98 – 111Combined sources14
Helixi114 – 134Combined sources21
Turni135 – 137Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10541-138[»]
3J6Yelectron microscopy6.10541-138[»]
3J77electron microscopy6.20641-138[»]
3J78electron microscopy6.30641-138[»]
3JCTelectron microscopy3.08M1-138[»]
4U3MX-ray3.00M4/m42-138[»]
4U3NX-ray3.20M4/m42-138[»]
4U3UX-ray2.90M4/m42-138[»]
4U4NX-ray3.10M4/m42-138[»]
4U4OX-ray3.60M4/m42-138[»]
4U4QX-ray3.00M4/m42-138[»]
4U4RX-ray2.80M4/m42-138[»]
4U4UX-ray3.00M4/m42-138[»]
4U4YX-ray3.20M4/m42-138[»]
4U4ZX-ray3.10M4/m42-138[»]
4U50X-ray3.20M4/m42-138[»]
4U51X-ray3.20M4/m42-138[»]
4U52X-ray3.00M4/m42-138[»]
4U53X-ray3.30M4/m42-138[»]
4U55X-ray3.20M4/m42-138[»]
4U56X-ray3.45M4/m42-138[»]
4U6FX-ray3.10M4/m42-138[»]
4V6Ielectron microscopy8.80BN1-138[»]
4V7Felectron microscopy8.70M1-138[»]
4V7RX-ray4.00BN/DN1-138[»]
4V88X-ray3.00BM/DM1-138[»]
4V8Telectron microscopy8.10M1-138[»]
5APNelectron microscopy3.91M1-138[»]
5APOelectron microscopy3.41M1-138[»]
5DATX-ray3.15M4/m42-138[»]
5DC3X-ray3.25M4/m42-138[»]
5DGEX-ray3.45M4/m42-138[»]
5DGFX-ray3.30M4/m42-138[»]
5DGVX-ray3.10M4/m42-138[»]
5FCIX-ray3.40M4/m42-138[»]
5FCJX-ray3.10M4/m42-138[»]
5FL8electron microscopy9.50M1-138[»]
5GAKelectron microscopy3.88O1-138[»]
5H4Pelectron microscopy3.07M1-138[»]
5I4LX-ray3.10M4/m42-138[»]
5JCSelectron microscopy9.50M1-138[»]
5JUOelectron microscopy4.00R1-138[»]
5JUPelectron microscopy3.50R1-138[»]
5JUSelectron microscopy4.20R1-138[»]
5JUTelectron microscopy4.00R1-138[»]
5JUUelectron microscopy4.00R1-138[»]
5LYBX-ray3.25M4/m42-138[»]
5M1Jelectron microscopy3.30M53-138[»]
5MC6electron microscopy3.80AM1-138[»]
5T62electron microscopy3.30O1-138[»]
5T6Relectron microscopy4.50O1-138[»]
5TGAX-ray3.30M4/m42-138[»]
5TGMX-ray3.50M4/m42-138[»]
ProteinModelPortaliP36105.
SMRiP36105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000201693.
InParanoidiP36105.
KOiK02875.
OMAiMAMKRIA.
OrthoDBiEOG092C5GEN.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiView protein in InterPro
IPR014722. Rib_L2_dom2.
IPR002784. Ribosomal_L14e_dom.
IPR008991. Translation_prot_SH3-like.
PfamiView protein in Pfam
PF01929. Ribosomal_L14e. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK
60 70 80 90 100
AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAAV CEKWAASSWA
110 120 130
KKIAQRERRA ALTDFERFQV MVLRKQKRYT VKKALAKA
Length:138
Mass (Da):15,167
Last modified:June 1, 1994 - v1
Checksum:iF8F2A346ABB395BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61398 Genomic DNA. No translation available.
Z28006 Genomic DNA. Translation: CAA81839.1.
Z28005 Genomic DNA. Translation: CAA81838.1.
S59773 Genomic DNA. Translation: AAC60550.1.
BK006944 Genomic DNA. Translation: DAA09151.1.
PIRiS30133.
RefSeqiNP_012920.1. NM_001179572.1.

Genome annotation databases

EnsemblFungiiYKL006W; YKL006W; YKL006W.
GeneIDi853864.
KEGGisce:YKL006W.

Similar proteinsi

Entry informationi

Entry nameiRL14A_YEAST
AccessioniPrimary (citable) accession number: P36105
Secondary accession number(s): D6VXT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: August 30, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names