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Protein

Transcription initiation factor IIE subunit alpha

Gene

TFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase (By similarity).By similarity

Miscellaneous

Present with 35900 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri124 – 152C4-typeSequence analysisAdd BLAST29

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II core binding Source: SGD
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31834-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Alternative name(s):
Factor A 66 kDa subunit
Transcription factor A large subunit
Gene namesi
Name:TFA1
Ordered Locus Names:YKL028W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL028W
SGDiS000001511 TFA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002112251 – 482Transcription initiation factor IIE subunit alphaAdd BLAST482

Proteomic databases

MaxQBiP36100
PaxDbiP36100
PRIDEiP36100

Interactioni

Subunit structurei

TFIIE is a tetramer of two alpha (TFA1) and two beta (TFA2) subunits.By similarity

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD

Protein-protein interaction databases

BioGridi34103, 171 interactors
DIPiDIP-1700N
IntActiP36100, 7 interactors
MINTiP36100
STRINGi4932.YKL028W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FMFelectron microscopy6.00R1-160[»]
5FYWelectron microscopy4.35W1-482[»]
5FZ5electron microscopy8.80W1-482[»]
5SVAelectron microscopy15.30h1-482[»]
ProteinModelPortaliP36100
SMRiP36100
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 99HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi374 – 482Asp/Glu-rich (highly acidic)Add BLAST109
Compositional biasi374 – 392Poly-GluAdd BLAST19

Sequence similaritiesi

Belongs to the TFIIE alpha subunit family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri124 – 152C4-typeSequence analysisAdd BLAST29

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000016696
HOGENOMiHOG000246773
InParanoidiP36100
KOiK03136
OMAiEFRCTYC
OrthoDBiEOG092C2M8E

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR017919 TFIIE/TFIIEa_HTH
IPR002853 TFIIE_asu
IPR024550 TFIIEa/SarR/Rpc3_HTH_dom
IPR013083 Znf_RING/FYVE/PHD
IPR013137 Znf_TFIIB
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF02002 TFIIE_alpha, 1 hit
SMARTiView protein in SMART
SM00531 TFIIE, 1 hit
PROSITEiView protein in PROSITE
PS51344 HTH_TFE_IIE, 1 hit

Sequencei

Sequence statusi: Complete.

P36100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN
60 70 80 90 100
KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW
110 120 130 140 150
KVHQVVQRLK DDLDKNSEPN GYMCPICLTK YTQLEAVQLL NFDRTEFLCS
160 170 180 190 200
LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ PIIDSLKKID DSRIEENTFE
210 220 230 240 250
IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM GTALGNDSSR
260 270 280 290 300
RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG
310 320 330 340 350
KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE
360 370 380 390 400
RENEKTLNDY YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN
410 420 430 440 450
DETARENALE DEFEDVTDTA GTAKTESNTS NDVKQESIND KTEDAVNATA
460 470 480
TASGPSANAK PNDGDDDDDD DDDEMDIEFE DV
Length:482
Mass (Da):54,742
Last modified:June 1, 1994 - v1
Checksum:i3E789DFC4247EF8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12825 Genomic DNA Translation: AAA62665.1
Z28028 Genomic DNA Translation: CAA81863.1
BK006944 Genomic DNA Translation: DAA09126.1
PIRiS37845
RefSeqiNP_012897.3, NM_001179594.3

Genome annotation databases

EnsemblFungiiYKL028W; YKL028W; YKL028W
GeneIDi853840
KEGGisce:YKL028W

Similar proteinsi

Entry informationi

Entry nameiT2EA_YEAST
AccessioniPrimary (citable) accession number: P36100
Secondary accession number(s): D6VXQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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