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Protein

Transmembrane E3 ubiquitin-protein ligase 1

Gene

TUL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase protein that mediates ubiquitination of vacuolar proteins such as CPS1, PPN1, PEP12 and other proteins containing exposed hydrophilic residues within their transmembrane domains (TMDs). Ubiquitination of such proteins mediates their sorting into internal vesicles in late endosomes.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri699 – 752RING-type; atypicalPROSITE-ProRule annotationAdd BLAST54

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein ubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31837-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane E3 ubiquitin-protein ligase 1 (EC:6.3.2.-)
Gene namesi
Name:TUL1
Ordered Locus Names:YKL034W
ORF Names:YKL247
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL034W.
SGDiS000001517. TUL1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 398LumenalSequence analysisAdd BLAST372
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Topological domaini420 – 431CytoplasmicSequence analysisAdd BLAST12
Transmembranei432 – 452HelicalSequence analysisAdd BLAST21
Topological domaini453 – 458LumenalSequence analysis6
Transmembranei459 – 479HelicalSequence analysisAdd BLAST21
Topological domaini480 – 523CytoplasmicSequence analysisAdd BLAST44
Transmembranei524 – 544HelicalSequence analysisAdd BLAST21
Topological domaini545 – 553LumenalSequence analysis9
Transmembranei554 – 574HelicalSequence analysisAdd BLAST21
Topological domaini575 – 602CytoplasmicSequence analysisAdd BLAST28
Transmembranei603 – 623HelicalSequence analysisAdd BLAST21
Topological domaini624 – 635LumenalSequence analysisAdd BLAST12
Transmembranei636 – 656HelicalSequence analysisAdd BLAST21
Topological domaini657 – 758CytoplasmicSequence analysisAdd BLAST102

GO - Cellular componenti

  • Dsc E3 ubiquitin ligase complex Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000005640927 – 758Transmembrane E3 ubiquitin-protein ligase 1Add BLAST732

Proteomic databases

MaxQBiP36096.
PRIDEiP36096.

Interactioni

Subunit structurei

Interacts with UBC4.1 Publication

Protein-protein interaction databases

BioGridi34097. 16 interactors.
DIPiDIP-5413N.
IntActiP36096. 1 interactor.
MINTiMINT-531834.

Structurei

3D structure databases

ProteinModelPortaliP36096.
SMRiP36096.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri699 – 752RING-type; atypicalPROSITE-ProRule annotationAdd BLAST54

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000248272.
InParanoidiP36096.
OMAiLENWMSY.
OrthoDBiEOG092C1ALL.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIDGNTLVF IIVILFLFFS SPGGDGVSSQ YEFNQLQRLK QQFRTEHNTF
60 70 80 90 100
VNMTYTDSFR NITGLKLSYQ DMLNNPLQNA TYPLPGKDYD RWFPNQNYMV
110 120 130 140 150
LPNDVIEAIN TEVWNTSNDD ASNLFPPNIT STLLGKIDLV SNNKYEKIRM
160 170 180 190 200
PVPRFYEPAT DFSEDIPPEG ETYWSEWPSY GELHNVSFQH GEIAIQISHM
210 220 230 240 250
SNLQDNNNYL RRNFINKKND RWKLLNLQID FSDKAEKEKH SIYSKAVYDI
260 270 280 290 300
QRGRILSISQ SSKFHSLFAL PHYMSFQNDY NEKIFNDVKE LVDEFWNFTD
310 320 330 340 350
YTDVMTMKDV QDAYNNANFK CEYLIFLQLE PWNQYTRDQI KLIDDELNWP
360 370 380 390 400
LGRPANLSSL PPINVVSGLL YSPDCGVRLG LHNVKGTRYE LKIMSIRKHL
410 420 430 440 450
LFGIALFAAQ IYLLLTQMHH TNTPSMVNKI SFYCFSMINL VDGSLATLYF
460 470 480 490 500
VAASVVPELY LPLVISAFSC FILASIFEIR YLISIYASQV NEQNVGIINL
510 520 530 540 550
LRGNTGTYDE NRPRPAFIPD EGSIGGSLYG RFFFMLIIFT FLILSSTSWP
560 570 580 590 600
RQLRMVFEYI LIFILNSYWI PQIFRNAVKG IPSRRERARS SIGGNRSQNK
610 620 630 640 650
MPLLWSFVIG TTIIRSLPVV YVFTYSSNVF RHHKDVHFVV FLSLWLLFQI
660 670 680 690 700
SILYSQDVLG SRWFLPKHTI PDGYSYFKPL SNEYISEHGG GTAEHTVDCA
710 720 730 740 750
ICMSDVPIYI EEIPETHKVD QHSYMVTPCN HVFHTSCLEN WMNYKLQCPV

CRSPLPPL
Length:758
Mass (Da):87,880
Last modified:June 1, 1994 - v1
Checksum:iF29A61E994F96628
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71622 Genomic DNA. No translation available.
X69584 Genomic DNA. Translation: CAA49298.1.
Z28034 Genomic DNA. Translation: CAA81869.1.
BK006944 Genomic DNA. Translation: DAA09121.1.
PIRiS37855.
RefSeqiNP_012890.1. NM_001179600.1.

Genome annotation databases

EnsemblFungiiYKL034W; YKL034W; YKL034W.
GeneIDi853832.
KEGGisce:YKL034W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71622 Genomic DNA. No translation available.
X69584 Genomic DNA. Translation: CAA49298.1.
Z28034 Genomic DNA. Translation: CAA81869.1.
BK006944 Genomic DNA. Translation: DAA09121.1.
PIRiS37855.
RefSeqiNP_012890.1. NM_001179600.1.

3D structure databases

ProteinModelPortaliP36096.
SMRiP36096.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34097. 16 interactors.
DIPiDIP-5413N.
IntActiP36096. 1 interactor.
MINTiMINT-531834.

Proteomic databases

MaxQBiP36096.
PRIDEiP36096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL034W; YKL034W; YKL034W.
GeneIDi853832.
KEGGisce:YKL034W.

Organism-specific databases

EuPathDBiFungiDB:YKL034W.
SGDiS000001517. TUL1.

Phylogenomic databases

HOGENOMiHOG000248272.
InParanoidiP36096.
OMAiLENWMSY.
OrthoDBiEOG092C1ALL.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-31837-MONOMER.

Miscellaneous databases

PROiP36096.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTUL1_YEAST
AccessioniPrimary (citable) accession number: P36096
Secondary accession number(s): D6VXQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.