P36095 (VPS24_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein-sorting-associated protein 24

Alternative name(s):
DOA4-independent degradation protein 3
ESCRT-III complex subunit VPS24

Gene names

Name:	VPS24
Synonyms:	DID3
Ordered Locus Names:	YKL041W
ORF Names:	YKL254

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment oflate-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III. Ref.5 Ref.6 Ref.7
Subunit structure	Core component of the ESCRT-III complex (endosomal sorting required for transport complex III). ESCRT-III appears to be sequentially assembled as a flat lattice on the endosome membrane and forms a transient 450 kDa complex that contains DID4, oligomerized SNF7, VPS20 and VPS24. SNF7 oligomerization into a membrane-associated filament is nucleated by association of SNF7 with VPS20; the process is terminated through association of VPS24, possibly by capping the SNF7 filament. VPS24 subsequently associates with DID4/VPS2. Interacts with the VPS4. Interacts with DID2. Ref.7 Ref.10
Subcellular location	Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note: Endosomal and other punctate structures. Ref.5 Ref.7 Ref.8
Miscellaneous	Present with 1890 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the SNF7 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intralumenal vesicle formation Inferred from mutant phenotype. Source: SGD late endosome to vacuole transport Inferred from mutant phenotype Ref.5. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Inferred by curator. Source: SGD
Cellular component	ESCRT III complex Inferred from direct assay Ref.7. Source: SGD endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Vacuolar protein-sorting-associated protein 24		PRO_0000211487

Sequences

	Sequence		Length	Mass (Da)
	P36095 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6073C1B680D58E98	FASTA	224	26,242
	10 20 30 40 50 60 MDYIKKAIWG PDPKEQQRRI RSVLRKNGRN IEKSLRELTV LQNKTQQLIK KSAKKNDVRT 70 80 90 100 110 120 VRLYAKELYQ INKQYDRMYT SRAQLDSVRM KIDEAIRMNT LSNQMADSAG LMREVNSLVR 130 140 150 160 170 180 LPQLRNTMIE LEKELMKSGI ISEMVDDTME SVGDVGEEMD EAVDEEVNKI VEQYTNEKFK 190 200 210 220 NVDQVPTVEL AANEEEQEIP DEKVDEEADR MVNEMRERLR ALQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames." Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A. Yeast 9:1379-1384(1993) [PubMed: 8154189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function." Babst M., Wendland B., Estepa E.J., Emr S.D. EMBO J. 17:2982-2993(1998) [PubMed: 9606181] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins." Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M. J. Cell Sci. 114:2395-2404(2001) [PubMed: 11559748] [Abstract] Cited for: FUNCTION.
[7]	"Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting." Babst M., Katzmann D.J., Estepa-Sabal E.J., Meerloo T., Emr S.D. Dev. Cell 3:271-282(2002) [PubMed: 12194857] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE ESCRT-III COMPLEX, INTERACTION WITH VPS2; VPS20; SNF7 AND VPS4, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes." Nickerson D.P., West M., Odorizzi G. J. Cell Biol. 175:715-720(2006) [PubMed: 17130288] [Abstract] Cited for: INTERACTION WITH DID2.
[11]	Erratum Nickerson D.P., West M., Odorizzi G. J. Cell Biol. 175:1043-1043(2006)
[12]	"Ordered assembly of the ESCRT-III complex on endosomes is required to sequester cargo during MVB formation." Teis D., Saksena S., Emr S.D. Dev. Cell 15:578-589(2008) [PubMed: 18854142] [Abstract] Cited for: ASSEMBLY OF THE ESCRT-III COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71621 Genomic DNA. Translation: CAA50631.1. Z28041 Genomic DNA. Translation: CAA81876.1. AY557901 Genomic DNA. Translation: AAS56227.1. BK006944 Genomic DNA. Translation: DAA09115.1.
PIR	S37862.
RefSeq	NP_012883.1. NM_001179607.1.
3D structure databases
ProteinModelPortal	P36095.
SMR	P36095. Positions 7-183.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7295N.
IntAct	P36095. 7 interactions.
MINT	MINT-468346.
STRING	P36095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL041W; YKL041W; YKL041W.
GeneID	853825.
KEGG	sce:YKL041W.
NMPDR	fig|4932.3.peg.3868.
Organism-specific databases
CYGD	YKL041w.
SGD	S000001524. VPS24.
Phylogenomic databases
eggNOG	fuNOG08501.
GeneTree	EFGT00050000003857.
HOGENOM	HBG713119.
OMA	KAVNRLY.
OrthoDB	EOG4F7RVD.
Gene expression databases
ArrayExpress	P36095.
Genevestigator	P36095.
GermOnline	YKL041W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005024. Snf7. [Graphical view]
KO	K12193.
Pfam	PF03357. Snf7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975014.

Entry information

Entry name

VPS24_YEAST

Accession

Primary (citable) accession number: P36095
Secondary accession number(s): D6VXP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program