Reviewed, UniProtKB/Swiss-Prot P36093 (PHD1_YEAST)

Names and origin

Protein names

Recommended name:
    Putative transcription factor PHD1

Gene names

Name:	PHD1
Ordered Locus Names:	YKL043W
ORF Names:	YKL256

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative transcription factor that functions in pseudohyphal growth.
Subcellular location	Nucleus.
Miscellaneous	Present with 1420 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the EFG1/PHD1/stuA family. Contains 1 HTH APSES-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	positive regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from mutant phenotype. Source: SGD pseudohyphal growth Ref.1 Inferred from mutant phenotype. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD transcription factor activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMT3	Q12306	1	EBI-13366,EBI-17490

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Putative transcription factor PHD1		PRO_0000058377
Regions
	Domain	186 – 292	107	HTH APSES-type
	DNA binding	220 – 241	22	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36093-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 22B6FA0D505D8654	FASTA	366	40,646
	10 20 30 40 50 60 MYHVPEMRLH YPLVNTQSNA AITPTRSYDN TLPSFNELSH QSTINLPFVQ RETPNAYANV 70 80 90 100 110 120 AQLATSPTQA KSGYYCRYYA VPFPTYPQQP QSPYQQAVLP YATIPNSNFQ PSSFPVMAVM 130 140 150 160 170 180 PPEVQFDGSF LNTLHPHTEL PPIIQNTNDT SVARPNNLKS IAAASPTVTA TTRTPGVSST 190 200 210 220 230 240 SVLKPRVITT MWEDENTICY QVEANGISVV RRADNNMING TKLLNVTKMT RGRRDGILRS 250 260 270 280 290 300 EKVREVVKIG SMHLKGVWIP FERAYILAQR EQILDHLYPL FVKDIESIVD ARKPSNKASL 310 320 330 340 350 360 TPKSSPAPIK QEPSDNKHEI ATEIKPKSID ALSNGASTQG AGELPHLKIN HIDTEAQTSR AKNELS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development." Gimeno C.J., Fink G.R. Mol. Cell. Biol. 14:2100-2112(1994) [PubMed: 8114741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S288c / YPH1.
[2]	"The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames." Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A. Yeast 9:1379-1384(1993) [PubMed: 8154189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Population structure of the wine yeast Saccharomyces cerevisiae." Townsend J.P., Aa E., Taylor J.W. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76625 / YPH499, YPS396, YPS400, YPS598, YPS600, YPS602, YPS604, YPS606, YPS608 and YPS610.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05241 Unassigned DNA. Translation: AAA18358.1. X71621 Genomic DNA. Translation: CAA50629.1. Z28043 Genomic DNA. Translation: CAA81878.1. AY557923 Genomic DNA. Translation: AAS56249.1. AY949939 Genomic DNA. Translation: AAY27341.1. AY949940 Genomic DNA. Translation: AAY27342.1. AY949941 Genomic DNA. Translation: AAY27343.1. AY949942 Genomic DNA. Translation: AAY27344.1. AY949943 Genomic DNA. Translation: AAY27345.1. AY949944 Genomic DNA. Translation: AAY27346.1. AY949945 Genomic DNA. Translation: AAY27347.1. AY949946 Genomic DNA. Translation: AAY27348.1. AY949947 Genomic DNA. Translation: AAY27349.1. AY949948 Genomic DNA. Translation: AAY27350.1.
PIR	S37864.
RefSeq	NP_012881.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4868N.
IntAct	P36093. 3 interactions.
Genome annotation databases
Ensembl	YKL043W. Saccharomyces cerevisiae. [Contig view]
GeneID	853823.
GenomeReviews	Gene locus YKL043W in contig Y13137_GR.
KEGG	sce:YKL043W.
NMPDR	fig|4932.3.peg.3866.
Organism-specific databases
CYGD	YKL043w.
SGD	S000001526. PHD1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P36093.
Gene expression databases
GermOnline	YKL043W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003163. APSES-type_HTH_DNA-bd. IPR018004. KilA_N/APSES_HTH. [Graphical view]
Gene3D	G3DSA:3.10.260.10. Mlu1-box-bd_prot_DNA-bd. 1 hit.
Pfam	PF04383. KilA-N. 1 hit. [Graphical view]
PROSITE	PS51299. HTH_APSES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975008.

Entry information

Entry name

PHD1_YEAST

Accession

Primary (citable) accession number: P36093
Secondary accession number(s): Q2VQ88

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)