ID   DCW1_YEAST              Reviewed;         449 AA.
AC   P36091; D6VXP1; Q66R60;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   27-MAR-2024, entry version 159.
DE   RecName: Full=Mannan endo-1,6-alpha-mannosidase DCW1;
DE            EC=3.2.1.101;
DE   AltName: Full=Defective cell wall 1;
DE   AltName: Full=Endo-alpha-1->6-D-mannanase DCW1;
DE   Flags: Precursor;
GN   Name=DCW1; OrderedLocusNames=YKL046C; ORFNames=YKL259;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8154189; DOI=10.1002/yea.320091212;
RA   Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.;
RT   "The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome
RT   XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a
RT   new gene encoding a putative histone and seven new open reading frames.";
RL   Yeast 9:1379-1384(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION, GLYCOSYLATION, AND SUBCELLULAR LOCATION.
RX   PubMed=12421307; DOI=10.1046/j.1365-2958.2002.03244.x;
RA   Kitagaki H., Wu H., Shimoi H., Ito K.;
RT   "Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell
RT   growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane
RT   proteins required for cell wall biogenesis in Saccharomyces cerevisiae.";
RL   Mol. Microbiol. 46:1011-1022(2002).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Required for normal synthesis of the cell wall.
CC       {ECO:0000269|PubMed:12421307}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in
CC         unbranched (1->6)-mannans.; EC=3.2.1.101;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12421307};
CC       Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:12421307}. Note=GPI-
CC       anchored plasma membrane protein (GPI-PMP).
CC   -!- MISCELLANEOUS: Present with 2580 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 76 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X71621; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; Z28046; CAA81881.1; -; Genomic_DNA.
DR   EMBL; AY723839; AAU09756.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09111.1; -; Genomic_DNA.
DR   PIR; S37867; S37867.
DR   RefSeq; NP_012878.1; NM_001179612.1.
DR   AlphaFoldDB; P36091; -.
DR   SMR; P36091; -.
DR   BioGRID; 34087; 94.
DR   IntAct; P36091; 2.
DR   MINT; P36091; -.
DR   STRING; 4932.YKL046C; -.
DR   CAZy; GH76; Glycoside Hydrolase Family 76.
DR   GlyCosmos; P36091; 12 sites, No reported glycans.
DR   GlyGen; P36091; 12 sites.
DR   PaxDb; 4932-YKL046C; -.
DR   PeptideAtlas; P36091; -.
DR   EnsemblFungi; YKL046C_mRNA; YKL046C; YKL046C.
DR   GeneID; 853820; -.
DR   KEGG; sce:YKL046C; -.
DR   AGR; SGD:S000001529; -.
DR   SGD; S000001529; DCW1.
DR   VEuPathDB; FungiDB:YKL046C; -.
DR   eggNOG; ENOG502QSWP; Eukaryota.
DR   HOGENOM; CLU_025694_1_2_1; -.
DR   InParanoid; P36091; -.
DR   OMA; QQSFKGY; -.
DR   OrthoDB; 2718577at2759; -.
DR   BioCyc; YEAST:G3O-31847-MONOMER; -.
DR   BioGRID-ORCS; 853820; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P36091; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P36091; Protein.
DR   GO; GO:0005933; C:cellular bud; HDA:SGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008496; F:mannan endo-1,6-alpha-mannosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0007117; P:budding cell bud growth; IGI:SGD.
DR   GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009272; P:fungal-type cell wall biogenesis; IDA:SGD.
DR   Gene3D; 1.50.10.20; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR005198; Glyco_hydro_76.
DR   InterPro; IPR014480; Mannan-1_6-alpha_mannosidase.
DR   PANTHER; PTHR12145:SF36; MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1; 1.
DR   PANTHER; PTHR12145; UNCHARACTERIZED; 1.
DR   Pfam; PF03663; Glyco_hydro_76; 1.
DR   PIRSF; PIRSF016302; Man_a_manosd; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Cell wall biogenesis/degradation; Glycoprotein; Glycosidase;
KW   GPI-anchor; Hydrolase; Lipoprotein; Membrane; Reference proteome; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..428
FT                   /note="Mannan endo-1,6-alpha-mannosidase DCW1"
FT                   /id="PRO_0000012125"
FT   PROPEP          429..449
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000012126"
FT   LIPID           428
FT                   /note="GPI-anchor amidated glycine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        34
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        84
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        133
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        203
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        225
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        240
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        265
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        281
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        337
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        362
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        420
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        276
FT                   /note="T -> A (in Ref. 4; AAU09756)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   449 AA;  49565 MW;  DECFE9CAFD9579D5 CRC64;
     MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN QYGKTVGMFS
     DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG DDNDYIPLNQ STTEGNDDQA
     FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA VFNTMALRWD ADSCGGGLRW QIFVWNSGYD
     YKNTVSNGAL FHIAARLARY TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN
     CTKVTSYQWT YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP
     NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG TDGHTCGLNW
     FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS SVGDGAAGTQ AQPTNLAPLN
     ITKGSKAGAG IITAVIGISI VACALWLVF
//