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Protein

Mannan endo-1,6-alpha-mannosidase DCW1

Gene

DCW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal synthesis of the cell wall.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

GO - Molecular functioni

GO - Biological processi

  • budding cell bud growth Source: SGD
  • carbohydrate catabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • filamentous growth Source: GO_Central
  • fungal-type cell wall biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-31847-MONOMER.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,6-alpha-mannosidase DCW1 (EC:3.2.1.101)
Alternative name(s):
Defective cell wall 1
Endo-alpha-1->6-D-mannanase DCW1
Gene namesi
Name:DCW1
Ordered Locus Names:YKL046C
ORF Names:YKL259
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL046C.
SGDiS000001529. DCW1.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

  • Note: GPI-anchored plasma membrane protein (GPI-PMP).

GO - Cellular componenti

  • anchored component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001212522 – 428Mannan endo-1,6-alpha-mannosidase DCW1Add BLAST407
PropeptideiPRO_0000012126429 – 449Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...)Sequence analysis1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Glycosylationi225N-linked (GlcNAc...)Sequence analysis1
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Glycosylationi337N-linked (GlcNAc...)Sequence analysis1
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1
Glycosylationi420N-linked (GlcNAc...)Sequence analysis1
Lipidationi428GPI-anchor amidated glycineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP36091.

Interactioni

Protein-protein interaction databases

BioGridi34087. 22 interactors.
IntActiP36091. 1 interactor.
MINTiMINT-4492510.

Structurei

3D structure databases

ProteinModelPortaliP36091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 76 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
InParanoidiP36091.
KOiK08257.
OMAiYERACEP.
OrthoDBiEOG092C22S6.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN
60 70 80 90 100
QYGKTVGMFS DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG
110 120 130 140 150
DDNDYIPLNQ STTEGNDDQA FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA
160 170 180 190 200
VFNTMALRWD ADSCGGGLRW QIFVWNSGYD YKNTVSNGAL FHIAARLARY
210 220 230 240 250
TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN CTKVTSYQWT
260 270 280 290 300
YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP
310 320 330 340 350
NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG
360 370 380 390 400
TDGHTCGLNW FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS
410 420 430 440
SVGDGAAGTQ AQPTNLAPLN ITKGSKAGAG IITAVIGISI VACALWLVF
Length:449
Mass (Da):49,565
Last modified:June 1, 1994 - v1
Checksum:iDECFE9CAFD9579D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276T → A in AAU09756 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1.
AY723839 Genomic DNA. Translation: AAU09756.1.
BK006944 Genomic DNA. Translation: DAA09111.1.
PIRiS37867.
RefSeqiNP_012878.1. NM_001179612.1.

Genome annotation databases

EnsemblFungiiYKL046C; YKL046C; YKL046C.
GeneIDi853820.
KEGGisce:YKL046C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1.
AY723839 Genomic DNA. Translation: AAU09756.1.
BK006944 Genomic DNA. Translation: DAA09111.1.
PIRiS37867.
RefSeqiNP_012878.1. NM_001179612.1.

3D structure databases

ProteinModelPortaliP36091.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34087. 22 interactors.
IntActiP36091. 1 interactor.
MINTiMINT-4492510.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Proteomic databases

PRIDEiP36091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL046C; YKL046C; YKL046C.
GeneIDi853820.
KEGGisce:YKL046C.

Organism-specific databases

EuPathDBiFungiDB:YKL046C.
SGDiS000001529. DCW1.

Phylogenomic databases

GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
InParanoidiP36091.
KOiK08257.
OMAiYERACEP.
OrthoDBiEOG092C22S6.

Enzyme and pathway databases

BioCyciYEAST:G3O-31847-MONOMER.

Miscellaneous databases

PROiP36091.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCW1_YEAST
AccessioniPrimary (citable) accession number: P36091
Secondary accession number(s): D6VXP1, Q66R60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.