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P36091

- DCW1_YEAST

UniProt

P36091 - DCW1_YEAST

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Protein

Mannan endo-1,6-alpha-mannosidase DCW1

Gene
DCW1, YKL046C, YKL259
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for normal synthesis of the cell wall.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

GO - Molecular functioni

  1. mannan endo-1,6-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. budding cell bud growth Source: SGD
  2. carbohydrate catabolic process Source: InterPro
  3. fungal-type cell wall biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-31847-MONOMER.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,6-alpha-mannosidase DCW1 (EC:3.2.1.101)
Alternative name(s):
Defective cell wall 1
Endo-alpha-1->6-D-mannanase DCW1
Gene namesi
Name:DCW1
Ordered Locus Names:YKL046C
ORF Names:YKL259
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL046c.
SGDiS000001529. DCW1.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor
Note: GPI-anchored plasma membrane protein (GPI-PMP).1 Publication

GO - Cellular componenti

  1. anchored component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed predictionAdd
BLAST
Chaini22 – 428407Mannan endo-1,6-alpha-mannosidase DCW1PRO_0000012125Add
BLAST
Propeptidei429 – 44921Removed in mature form Reviewed predictionPRO_0000012126Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi84 – 841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi109 – 1091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi133 – 1331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi203 – 2031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi225 – 2251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi240 – 2401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi265 – 2651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi281 – 2811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi337 – 3371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi362 – 3621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi420 – 4201N-linked (GlcNAc...) Reviewed prediction
Lipidationi428 – 4281GPI-anchor amidated glycine Reviewed prediction

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP36091.
PeptideAtlasiP36091.

Expressioni

Gene expression databases

GenevestigatoriP36091.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-2052781,EBI-2357
CHO1P084561EBI-2052781,EBI-14055
OST1P415431EBI-2052781,EBI-12651
PMP2P409751EBI-2052781,EBI-2043041
SWP1Q027951EBI-2052781,EBI-12666

Protein-protein interaction databases

BioGridi34087. 24 interactions.
IntActiP36091. 1 interaction.
MINTiMINT-4492510.
STRINGi4932.YKL046C.

Structurei

3D structure databases

ProteinModelPortaliP36091.
SMRiP36091. Positions 144-331.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG74795.
GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
KOiK08257.
OMAiRSFKAYF.
OrthoDBiEOG72RN80.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36091-1 [UniParc]FASTAAdd to Basket

« Hide

MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN    50
QYGKTVGMFS DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG 100
DDNDYIPLNQ STTEGNDDQA FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA 150
VFNTMALRWD ADSCGGGLRW QIFVWNSGYD YKNTVSNGAL FHIAARLARY 200
TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN CTKVTSYQWT 250
YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP 300
NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG 350
TDGHTCGLNW FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS 400
SVGDGAAGTQ AQPTNLAPLN ITKGSKAGAG IITAVIGISI VACALWLVF 449
Length:449
Mass (Da):49,565
Last modified:June 1, 1994 - v1
Checksum:iDECFE9CAFD9579D5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti276 – 2761T → A in AAU09756. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1.
AY723839 Genomic DNA. Translation: AAU09756.1.
BK006944 Genomic DNA. Translation: DAA09111.1.
PIRiS37867.
RefSeqiNP_012878.1. NM_001179612.1.

Genome annotation databases

EnsemblFungiiYKL046C; YKL046C; YKL046C.
GeneIDi853820.
KEGGisce:YKL046C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1 .
AY723839 Genomic DNA. Translation: AAU09756.1 .
BK006944 Genomic DNA. Translation: DAA09111.1 .
PIRi S37867.
RefSeqi NP_012878.1. NM_001179612.1.

3D structure databases

ProteinModelPortali P36091.
SMRi P36091. Positions 144-331.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34087. 24 interactions.
IntActi P36091. 1 interaction.
MINTi MINT-4492510.
STRINGi 4932.YKL046C.

Protein family/group databases

CAZyi GH76. Glycoside Hydrolase Family 76.

Proteomic databases

PaxDbi P36091.
PeptideAtlasi P36091.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL046C ; YKL046C ; YKL046C .
GeneIDi 853820.
KEGGi sce:YKL046C.

Organism-specific databases

CYGDi YKL046c.
SGDi S000001529. DCW1.

Phylogenomic databases

eggNOGi NOG74795.
GeneTreei ENSGT00390000002859.
HOGENOMi HOG000178818.
KOi K08257.
OMAi RSFKAYF.
OrthoDBi EOG72RN80.

Enzyme and pathway databases

BioCyci YEAST:G3O-31847-MONOMER.

Miscellaneous databases

NextBioi 975002.

Gene expression databases

Genevestigatori P36091.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view ]
Pfami PF03663. Glyco_hydro_76. 1 hit.
[Graphical view ]
PIRSFi PIRSF016302. Man_a_manosd. 1 hit.
SUPFAMi SSF48208. SSF48208. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames."
    Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.
    Yeast 9:1379-1384(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae."
    Kitagaki H., Wu H., Shimoi H., Ito K.
    Mol. Microbiol. 46:1011-1022(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDCW1_YEAST
AccessioniPrimary (citable) accession number: P36091
Secondary accession number(s): D6VXP1, Q66R60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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