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Protein

Mannan endo-1,6-alpha-mannosidase DCW1

Gene

DCW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for normal synthesis of the cell wall.1 Publication

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

GO - Molecular functioni

GO - Biological processi

  • budding cell bud growth Source: SGD
  • carbohydrate catabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • filamentous growth Source: GO_Central
  • fungal-type cell wall biogenesis Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-31847-MONOMER

Protein family/group databases

CAZyiGH76 Glycoside Hydrolase Family 76

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,6-alpha-mannosidase DCW1 (EC:3.2.1.101)
Alternative name(s):
Defective cell wall 1
Endo-alpha-1->6-D-mannanase DCW1
Gene namesi
Name:DCW1
Ordered Locus Names:YKL046C
ORF Names:YKL259
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL046C
SGDiS000001529 DCW1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001212522 – 428Mannan endo-1,6-alpha-mannosidase DCW1Add BLAST407
PropeptideiPRO_0000012126429 – 449Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi203N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi265N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi281N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi337N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi362N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi420N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi428GPI-anchor amidated glycineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP36091
PRIDEiP36091

Interactioni

Protein-protein interaction databases

BioGridi34087, 90 interactors
IntActiP36091, 1 interactor
MINTiP36091
STRINGi4932.YKL046C

Structurei

3D structure databases

ProteinModelPortaliP36091
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 76 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000002859
HOGENOMiHOG000178818
InParanoidiP36091
KOiK08257
OMAiWTYNSGV
OrthoDBiEOG092C22S6

Family and domain databases

InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR005198 Glyco_hydro_76
IPR014480 Mannan-1_6-alpha_mannosidase
PANTHERiPTHR12145 PTHR12145, 1 hit
PfamiView protein in Pfam
PF03663 Glyco_hydro_76, 1 hit
PIRSFiPIRSF016302 Man_a_manosd, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN
60 70 80 90 100
QYGKTVGMFS DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG
110 120 130 140 150
DDNDYIPLNQ STTEGNDDQA FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA
160 170 180 190 200
VFNTMALRWD ADSCGGGLRW QIFVWNSGYD YKNTVSNGAL FHIAARLARY
210 220 230 240 250
TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN CTKVTSYQWT
260 270 280 290 300
YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP
310 320 330 340 350
NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG
360 370 380 390 400
TDGHTCGLNW FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS
410 420 430 440
SVGDGAAGTQ AQPTNLAPLN ITKGSKAGAG IITAVIGISI VACALWLVF
Length:449
Mass (Da):49,565
Last modified:June 1, 1994 - v1
Checksum:iDECFE9CAFD9579D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276T → A in AAU09756 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA No translation available.
Z28046 Genomic DNA Translation: CAA81881.1
AY723839 Genomic DNA Translation: AAU09756.1
BK006944 Genomic DNA Translation: DAA09111.1
PIRiS37867
RefSeqiNP_012878.1, NM_001179612.1

Genome annotation databases

EnsemblFungiiYKL046C; YKL046C; YKL046C
GeneIDi853820
KEGGisce:YKL046C

Similar proteinsi

Entry informationi

Entry nameiDCW1_YEAST
AccessioniPrimary (citable) accession number: P36091
Secondary accession number(s): D6VXP1, Q66R60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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