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P36091

- DCW1_YEAST

UniProt

P36091 - DCW1_YEAST

Protein

Mannan endo-1,6-alpha-mannosidase DCW1

Gene

DCW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Required for normal synthesis of the cell wall.1 Publication

    Catalytic activityi

    Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

    GO - Molecular functioni

    1. mannan endo-1,6-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. budding cell bud growth Source: SGD
    2. carbohydrate catabolic process Source: InterPro
    3. fungal-type cell wall biogenesis Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31847-MONOMER.

    Protein family/group databases

    CAZyiGH76. Glycoside Hydrolase Family 76.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,6-alpha-mannosidase DCW1 (EC:3.2.1.101)
    Alternative name(s):
    Defective cell wall 1
    Endo-alpha-1->6-D-mannanase DCW1
    Gene namesi
    Name:DCW1
    Ordered Locus Names:YKL046C
    ORF Names:YKL259
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL046c.
    SGDiS000001529. DCW1.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication
    Note: GPI-anchored plasma membrane protein (GPI-PMP).

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 428407Mannan endo-1,6-alpha-mannosidase DCW1PRO_0000012125Add
    BLAST
    Propeptidei429 – 44921Removed in mature formSequence AnalysisPRO_0000012126Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence Analysis
    Lipidationi428 – 4281GPI-anchor amidated glycineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP36091.
    PeptideAtlasiP36091.

    Expressioni

    Gene expression databases

    GenevestigatoriP36091.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-2052781,EBI-2357
    CHO1P084561EBI-2052781,EBI-14055
    OST1P415431EBI-2052781,EBI-12651
    PMP2P409751EBI-2052781,EBI-2043041
    SWP1Q027951EBI-2052781,EBI-12666

    Protein-protein interaction databases

    BioGridi34087. 24 interactions.
    IntActiP36091. 1 interaction.
    MINTiMINT-4492510.
    STRINGi4932.YKL046C.

    Structurei

    3D structure databases

    ProteinModelPortaliP36091.
    SMRiP36091. Positions 144-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 76 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG74795.
    GeneTreeiENSGT00390000002859.
    HOGENOMiHOG000178818.
    KOiK08257.
    OMAiRSFKAYF.
    OrthoDBiEOG72RN80.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR005198. Glyco_hydro_76.
    IPR014480. Mannan-1_6-alpha_mannosidase.
    [Graphical view]
    PfamiPF03663. Glyco_hydro_76. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36091-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN    50
    QYGKTVGMFS DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG 100
    DDNDYIPLNQ STTEGNDDQA FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA 150
    VFNTMALRWD ADSCGGGLRW QIFVWNSGYD YKNTVSNGAL FHIAARLARY 200
    TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN CTKVTSYQWT 250
    YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP 300
    NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG 350
    TDGHTCGLNW FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS 400
    SVGDGAAGTQ AQPTNLAPLN ITKGSKAGAG IITAVIGISI VACALWLVF 449
    Length:449
    Mass (Da):49,565
    Last modified:June 1, 1994 - v1
    Checksum:iDECFE9CAFD9579D5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti276 – 2761T → A in AAU09756. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71621 Genomic DNA. No translation available.
    Z28046 Genomic DNA. Translation: CAA81881.1.
    AY723839 Genomic DNA. Translation: AAU09756.1.
    BK006944 Genomic DNA. Translation: DAA09111.1.
    PIRiS37867.
    RefSeqiNP_012878.1. NM_001179612.1.

    Genome annotation databases

    EnsemblFungiiYKL046C; YKL046C; YKL046C.
    GeneIDi853820.
    KEGGisce:YKL046C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71621 Genomic DNA. No translation available.
    Z28046 Genomic DNA. Translation: CAA81881.1 .
    AY723839 Genomic DNA. Translation: AAU09756.1 .
    BK006944 Genomic DNA. Translation: DAA09111.1 .
    PIRi S37867.
    RefSeqi NP_012878.1. NM_001179612.1.

    3D structure databases

    ProteinModelPortali P36091.
    SMRi P36091. Positions 144-331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34087. 24 interactions.
    IntActi P36091. 1 interaction.
    MINTi MINT-4492510.
    STRINGi 4932.YKL046C.

    Protein family/group databases

    CAZyi GH76. Glycoside Hydrolase Family 76.

    Proteomic databases

    PaxDbi P36091.
    PeptideAtlasi P36091.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL046C ; YKL046C ; YKL046C .
    GeneIDi 853820.
    KEGGi sce:YKL046C.

    Organism-specific databases

    CYGDi YKL046c.
    SGDi S000001529. DCW1.

    Phylogenomic databases

    eggNOGi NOG74795.
    GeneTreei ENSGT00390000002859.
    HOGENOMi HOG000178818.
    KOi K08257.
    OMAi RSFKAYF.
    OrthoDBi EOG72RN80.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31847-MONOMER.

    Miscellaneous databases

    NextBioi 975002.

    Gene expression databases

    Genevestigatori P36091.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR005198. Glyco_hydro_76.
    IPR014480. Mannan-1_6-alpha_mannosidase.
    [Graphical view ]
    Pfami PF03663. Glyco_hydro_76. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016302. Man_a_manosd. 1 hit.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames."
      Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.
      Yeast 9:1379-1384(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae."
      Kitagaki H., Wu H., Shimoi H., Ito K.
      Mol. Microbiol. 46:1011-1022(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDCW1_YEAST
    AccessioniPrimary (citable) accession number: P36091
    Secondary accession number(s): D6VXP1, Q66R60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2580 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3