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Protein

Mannan endo-1,6-alpha-mannosidase DCW1

Gene

DCW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal synthesis of the cell wall.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

GO - Molecular functioni

GO - Biological processi

  • budding cell bud growth Source: SGD
  • carbohydrate catabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • fungal-type cell wall biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-31847-MONOMER.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,6-alpha-mannosidase DCW1 (EC:3.2.1.101)
Alternative name(s):
Defective cell wall 1
Endo-alpha-1->6-D-mannanase DCW1
Gene namesi
Name:DCW1
Ordered Locus Names:YKL046C
ORF Names:YKL259
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL046C.
SGDiS000001529. DCW1.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

  • Note: GPI-anchored plasma membrane protein (GPI-PMP).

GO - Cellular componenti

  • anchored component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 428407Mannan endo-1,6-alpha-mannosidase DCW1PRO_0000012125Add
BLAST
Propeptidei429 – 44921Removed in mature formSequence analysisPRO_0000012126Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence analysis
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence analysis
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence analysis
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence analysis
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence analysis
Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence analysis
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence analysis
Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence analysis
Lipidationi428 – 4281GPI-anchor amidated glycineSequence analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Protein-protein interaction databases

BioGridi34087. 22 interactions.
IntActiP36091. 1 interaction.
MINTiMINT-4492510.

Structurei

3D structure databases

ProteinModelPortaliP36091.
SMRiP36091. Positions 144-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 76 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
InParanoidiP36091.
KOiK08257.
OMAiYERACEP.
OrthoDBiEOG092C22S6.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN
60 70 80 90 100
QYGKTVGMFS DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG
110 120 130 140 150
DDNDYIPLNQ STTEGNDDQA FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA
160 170 180 190 200
VFNTMALRWD ADSCGGGLRW QIFVWNSGYD YKNTVSNGAL FHIAARLARY
210 220 230 240 250
TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN CTKVTSYQWT
260 270 280 290 300
YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP
310 320 330 340 350
NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG
360 370 380 390 400
TDGHTCGLNW FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS
410 420 430 440
SVGDGAAGTQ AQPTNLAPLN ITKGSKAGAG IITAVIGISI VACALWLVF
Length:449
Mass (Da):49,565
Last modified:June 1, 1994 - v1
Checksum:iDECFE9CAFD9579D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti276 – 2761T → A in AAU09756 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1.
AY723839 Genomic DNA. Translation: AAU09756.1.
BK006944 Genomic DNA. Translation: DAA09111.1.
PIRiS37867.
RefSeqiNP_012878.1. NM_001179612.1.

Genome annotation databases

EnsemblFungiiYKL046C; YKL046C; YKL046C.
GeneIDi853820.
KEGGisce:YKL046C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1.
AY723839 Genomic DNA. Translation: AAU09756.1.
BK006944 Genomic DNA. Translation: DAA09111.1.
PIRiS37867.
RefSeqiNP_012878.1. NM_001179612.1.

3D structure databases

ProteinModelPortaliP36091.
SMRiP36091. Positions 144-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34087. 22 interactions.
IntActiP36091. 1 interaction.
MINTiMINT-4492510.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL046C; YKL046C; YKL046C.
GeneIDi853820.
KEGGisce:YKL046C.

Organism-specific databases

EuPathDBiFungiDB:YKL046C.
SGDiS000001529. DCW1.

Phylogenomic databases

GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
InParanoidiP36091.
KOiK08257.
OMAiYERACEP.
OrthoDBiEOG092C22S6.

Enzyme and pathway databases

BioCyciYEAST:G3O-31847-MONOMER.

Miscellaneous databases

PROiP36091.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCW1_YEAST
AccessioniPrimary (citable) accession number: P36091
Secondary accession number(s): D6VXP1, Q66R60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.