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P36091 (DCW1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,6-alpha-mannosidase DCW1
EC=3.2.1.101
Alternative name(s):
Defective cell wall 1
Endo-alpha-1->6-D-mannanase DCW1
Gene names
Name:DCW1
Ordered Locus Names:YKL046C
ORF Names:YKL259
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for normal synthesis of the cell wall. Ref.5

Catalytic activity

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor. Note: GPI-anchored plasma membrane protein (GPI-PMP). Ref.5

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyl hydrolase 76 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 428407Mannan endo-1,6-alpha-mannosidase DCW1
PRO_0000012125
Propeptide429 – 44921Removed in mature form Potential
PRO_0000012126

Amino acid modifications

Modified residue4001Phosphoserine Ref.7
Modified residue4011Phosphoserine Ref.7
Lipidation4281GPI-anchor amidated glycine Potential
Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2251N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation2651N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation3371N-linked (GlcNAc...) Potential
Glycosylation3621N-linked (GlcNAc...) Potential
Glycosylation4201N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2761T → A in AAU09756. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P36091 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: DECFE9CAFD9579D5

FASTA44949,565
        10         20         30         40         50         60 
MLVNKVIGLL GVLFATRFTN AVELDLDNYE SLQNATSLIA YGLMDYYTGN QYGKTVGMFS 

        70         80         90        100        110        120 
DPYYWWEAGG AWGCMLDYWF FMDNDTYNDE IIAAMIHQAG DDNDYIPLNQ STTEGNDDQA 

       130        140        150        160        170        180 
FWGIAAMTAA ERNFTNPPEN EPQWLYLAQA VFNTMALRWD ADSCGGGLRW QIFVWNSGYD 

       190        200        210        220        230        240 
YKNTVSNGAL FHIAARLARY TGNQTYVDWA EKVYEWMVGV NLISNGTYKY VYDGVSIDDN 

       250        260        270        280        290        300 
CTKVTSYQWT YNQGLLLAGS AYLYNFTGSD LWHTRTKEFL NASQVFFHDG IVYEAACQGP 

       310        320        330        340        350        360 
NSCNTDQRSF KAYFARFLGV TAQLVPETRN QIMSWLNTSA IAAAKSCSGG TDGHTCGLNW 

       370        380        390        400        410        420 
FNGTWDGMYG LGEQMSALEV MVNTRALDKP APYTAENGGS SVGDGAAGTQ AQPTNLAPLN 

       430        440 
ITKGSKAGAG IITAVIGISI VACALWLVF

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames."
Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.
Yeast 9:1379-1384(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae."
Kitagaki H., Wu H., Shimoi H., Ito K.
Mol. Microbiol. 46:1011-1022(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400 AND SER-401, MASS SPECTROMETRY.
Strain: SUB592.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X71621 Genomic DNA. No translation available.
Z28046 Genomic DNA. Translation: CAA81881.1.
AY723839 Genomic DNA. Translation: AAU09756.1.
BK006944 Genomic DNA. Translation: DAA09111.1.
PIRS37867.
RefSeqNP_012878.1. NM_001179612.1.

3D structure databases

ProteinModelPortalP36091.
SMRP36091. Positions 144-331.
ModBaseSearch...

Protein-protein interaction databases

IntActP36091. 1 interaction.
MINTMINT-4492510.
STRING4932.YKL046C.

Protein family/group databases

CAZyGH76. Glycoside Hydrolase Family 76.

Proteomic databases

PaxDbP36091.
PeptideAtlasP36091.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL046C; YKL046C; YKL046C.
GeneID853820.
KEGGsce:YKL046C.

Organism-specific databases

CYGDYKL046c.
SGDS000001529. DCW1.

Phylogenomic databases

eggNOGNOG74795.
GeneTreeENSGT00390000002859.
HOGENOMHOG000178818.
KOK08257.
OMAQRSFKAY.
OrthoDBEOG4VT95S.

Gene expression databases

GenevestigatorP36091.
GermOnlineYKL046C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMSSF48208. Glyco_trans_6hp. 1 hit.
ProtoNetSearch...

Other

NextBio975002.

Entry information

Entry nameDCW1_YEAST
AccessionPrimary (citable) accession number: P36091
Secondary accession number(s): D6VXP1, Q66R60
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents