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Protein

Free methionine-R-sulfoxide reductase

Gene

YKL069W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation-reduction of the R-enantiomer of free methionine sulfoxide to methionine. Does not act on S-enantiomer of free methionine sulfoxide or R-enantiomer of dabsylated methionine sulfoxide. Involved in protection against oxidative stress.1 Publication

Catalytic activityi

L-methionine + thioredoxin disulfide + H2O = L-methionine (R)-S-oxide + thioredoxin.1 Publication

Kineticsi

  1. KM=230 µM for free methionine-R-sulfoxide1 Publication
  1. Vmax=443 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-31865-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Free methionine-R-sulfoxide reductase (EC:1.8.4.14)
Short name:
fRMsr
Alternative name(s):
GAF domain-containing protein YKL069W
Gene namesi
Ordered Locus Names:YKL069W
ORF Names:YKL340
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL069W.
SGDiS000001552. YKL069W.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Single deletion of fRMsr has complete growth inhibition on methionine-R-sulfoxide medium and fRMsr and MXR1 double deletion completely blocks the growth on both methionine-R-sulfoxide and methionine-S-sulfoxide medium. FRMsr and MXR2 double deletion has no effect on growth on methionine-S-sulfoxide medium. Single mutant or any of the double mutants show no growth defects in methionine medium, even the fRMsr, MXR1 and MXR2 triple deletion mutant is viable and grows similarly to wild-type. Single deletion of fRMsr has an increased sensitivity to oxidative stress and a decreased life span of 18% compared to wild-type. FRMsr and MXR1, as well as fRMsr and MXR2 double mutants, and fRMsr, MXR1 and MXR2 triple mutant show 20% reduction in life span compared with wild-type cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91C → S: Dramatic reduction in enzyme activity. 1 Publication1
Mutagenesisi101C → S: Dramatic reduction in enzyme activity. 1 Publication1
Mutagenesisi125C → S: Dramatic reduction in enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001715521 – 180Free methionine-R-sulfoxide reductaseAdd BLAST180

Proteomic databases

MaxQBiP36088.
PRIDEiP36088.

Interactioni

Protein-protein interaction databases

BioGridi34063. 129 interactors.
DIPiDIP-4301N.
IntActiP36088. 4 interactors.
MINTiMINT-523111.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 13Combined sources5
Helixi21 – 36Combined sources16
Helixi42 – 59Combined sources18
Beta strandi64 – 72Combined sources9
Beta strandi74 – 77Combined sources4
Beta strandi79 – 88Combined sources10
Beta strandi92 – 95Combined sources4
Helixi99 – 107Combined sources9
Beta strandi111 – 114Combined sources4
Helixi116 – 118Combined sources3
Beta strandi131 – 138Combined sources8
Beta strandi144 – 154Combined sources11
Helixi160 – 176Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F5MX-ray1.90A/B1-180[»]
3KO6X-ray2.55A/B1-180[»]
ProteinModelPortaliP36088.
SMRiP36088.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36088.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 177GAFAdd BLAST79

Sequence similaritiesi

Contains 1 GAF domain.Curated

Phylogenomic databases

HOGENOMiHOG000022909.
InParanoidiP36088.
KOiK08968.
OMAiLLWHAYK.
OrthoDBiEOG092C4PVO.

Family and domain databases

Gene3Di3.30.450.40. 1 hit.
InterProiIPR000614. fRMsr_CS.
IPR003018. GAF.
IPR029016. GAF_dom-like.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
PROSITEiPS01320. UPF0067. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36088-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSTGFHHA DHVNYSSNLN KEEILEQLLL SYEGLSDGQV NWVCNLSNAS
60 70 80 90 100
SLIWHAYKSL AVDINWAGFY VTQASEENTL ILGPFQGKVA CQMIQFGKGV
110 120 130 140 150
CGTAASTKET QIVPDVNKYP GHIACDGETK SEIVVPIISN DGKTLGVIDI
160 170 180
DCLDYEGFDH VDKEFLEKLA KLINKSCVFK
Length:180
Mass (Da):19,734
Last modified:June 1, 1994 - v1
Checksum:i911158E8C0F2B0A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75780 Genomic DNA. Translation: CAA53405.1.
Z28069 Genomic DNA. Translation: CAA81906.1.
BK006944 Genomic DNA. Translation: DAA09087.1.
PIRiS37891.
RefSeqiNP_012854.1. NM_001179635.1.

Genome annotation databases

EnsemblFungiiYKL069W; YKL069W; YKL069W.
GeneIDi853794.
KEGGisce:YKL069W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75780 Genomic DNA. Translation: CAA53405.1.
Z28069 Genomic DNA. Translation: CAA81906.1.
BK006944 Genomic DNA. Translation: DAA09087.1.
PIRiS37891.
RefSeqiNP_012854.1. NM_001179635.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F5MX-ray1.90A/B1-180[»]
3KO6X-ray2.55A/B1-180[»]
ProteinModelPortaliP36088.
SMRiP36088.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34063. 129 interactors.
DIPiDIP-4301N.
IntActiP36088. 4 interactors.
MINTiMINT-523111.

Proteomic databases

MaxQBiP36088.
PRIDEiP36088.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL069W; YKL069W; YKL069W.
GeneIDi853794.
KEGGisce:YKL069W.

Organism-specific databases

EuPathDBiFungiDB:YKL069W.
SGDiS000001552. YKL069W.

Phylogenomic databases

HOGENOMiHOG000022909.
InParanoidiP36088.
KOiK08968.
OMAiLLWHAYK.
OrthoDBiEOG092C4PVO.

Enzyme and pathway databases

BioCyciYEAST:G3O-31865-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP36088.
PROiP36088.

Family and domain databases

Gene3Di3.30.450.40. 1 hit.
InterProiIPR000614. fRMsr_CS.
IPR003018. GAF.
IPR029016. GAF_dom-like.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
PROSITEiPS01320. UPF0067. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRMSR_YEAST
AccessioniPrimary (citable) accession number: P36088
Secondary accession number(s): D6VXL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.