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Protein

Splicing factor MUD2

Gene

MUD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor that contacts pre-mRNA directly and is a component of the pre-mRNA-U1 snRNP complex (commitment complex 2) that forms during early spliceosome assembly in yeast extracts.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • pre-mRNA branch point binding Source: SGD

GO - Biological processi

  • mRNA branch site recognition Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor MUD2
Gene namesi
Name:MUD2
Ordered Locus Names:YKL074C
ORF Names:YKL358
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL074C.
SGDiS000001557. MUD2.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Splicing factor MUD2PRO_0000081654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36084.
PeptideAtlasiP36084.

PTM databases

iPTMnetiP36084.

Interactioni

Subunit structurei

MSL5, MUD2 and PRP40 interact to form the commitment complex 2 (CC2), a precursor of mature spliceosomes.

Binary interactionsi

WithEntry#Exp.IntActNotes
MSL5Q121865EBI-11612,EBI-34012

Protein-protein interaction databases

BioGridi34057. 161 interactions.
DIPiDIP-758N.
IntActiP36084. 11 interactions.
MINTiMINT-2786506.

Structurei

3D structure databases

ProteinModelPortaliP36084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini424 – 51188RRMAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

InParanoidiP36084.
KOiK12837.
OMAiDYRINID.
OrthoDBiEOG7SFJ5R.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR003954. RRM_dom_euk.
[Graphical view]
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequencei

Sequence statusi: Complete.

P36084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEKRLEDL RSKIMESIGK SEKDVVPIEN KRFNTDNAVI DTHFKRQKSD
60 70 80 90 100
GELPKAPKSR NVSHSNNRGP SSIITMSTNR TTYEQTRAGP HRQSYRDASG
110 120 130 140 150
RSYNRENRYS SHNTGPQWNN NPYNRQRDER RGRNERFDRR GRNGNGNYDR
160 170 180 190 200
FNYQRKNEGS KFNGDRDKRQ LQTNKYDMNY NSQNVMYPGS SFDSPAYYNM
210 220 230 240 250
ASSKANSRLV ISGLSQSSDP SIVARLKDLL ENFISGLQKT ESNAEDFKIS
260 270 280 290 300
NFYIGEGRPD HIIVEFSSQI CSTMVLACRS FFNAKLGTFD LKWRRPNDYV
310 320 330 340 350
QQLDHLVDFC RGTVIALENL ENIGEGEDYR MKELFSSLNV TNGTAKPLFY
360 370 380 390 400
KCSSNTNNTG KESEFTKCIL LSFEVVTQDI LDKLKPYKWF KPNDGKISQV
410 420 430 440 450
TSWITFQSLP NLVTQSVRVE SRVLLLLNCL DPLDLKDETF ITEIKETLKY
460 470 480 490 500
SIAGADTIKI CQPGVDYRLN FENLASGAGN IYIKFKTLEA AKHAMEELPG
510 520
TQFNDRTVLC TYIDEDDFDM MEATQLS
Length:527
Mass (Da):60,460
Last modified:August 16, 2004 - v3
Checksum:i884427AB57A12E12
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981A → R in AAA64215 (PubMed:7926772).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22449 Genomic DNA. Translation: AAA64215.1.
X75780 Genomic DNA. Translation: CAA53400.1.
Z28074 Genomic DNA. Translation: CAA81911.1.
BK006944 Genomic DNA. Translation: DAA09082.1.
PIRiS37899.
RefSeqiNP_012849.1. NM_001179640.1.

Genome annotation databases

EnsemblFungiiYKL074C; YKL074C; YKL074C.
GeneIDi853788.
KEGGisce:YKL074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22449 Genomic DNA. Translation: AAA64215.1.
X75780 Genomic DNA. Translation: CAA53400.1.
Z28074 Genomic DNA. Translation: CAA81911.1.
BK006944 Genomic DNA. Translation: DAA09082.1.
PIRiS37899.
RefSeqiNP_012849.1. NM_001179640.1.

3D structure databases

ProteinModelPortaliP36084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34057. 161 interactions.
DIPiDIP-758N.
IntActiP36084. 11 interactions.
MINTiMINT-2786506.

PTM databases

iPTMnetiP36084.

Proteomic databases

MaxQBiP36084.
PeptideAtlasiP36084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL074C; YKL074C; YKL074C.
GeneIDi853788.
KEGGisce:YKL074C.

Organism-specific databases

EuPathDBiFungiDB:YKL074C.
SGDiS000001557. MUD2.

Phylogenomic databases

InParanoidiP36084.
KOiK12837.
OMAiDYRINID.
OrthoDBiEOG7SFJ5R.

Enzyme and pathway databases

BioCyciYEAST:G3O-31870-MONOMER.

Miscellaneous databases

PROiP36084.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR003954. RRM_dom_euk.
[Graphical view]
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition."
    Abovich N., Liao X.C., Rosbash M.
    Genes Dev. 8:843-854(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins."
    Rasmussen S.W.
    Yeast 10:S69-S74(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-365.
    Strain: ATCC 204508 / S288c.
  5. "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals."
    Abovich N., Rosbash M.
    Cell 89:403-412(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MSL5 AND PRP40.
  6. "Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly."
    Rutz B., Seraphin B.
    RNA 5:819-831(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MSL5.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMUD2_YEAST
AccessioniPrimary (citable) accession number: P36084
Secondary accession number(s): D6VXL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.