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Protein

Splicing factor MUD2

Gene

MUD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor that contacts pre-mRNA directly and is a component of the pre-mRNA-U1 snRNP complex (commitment complex 2) that forms during early spliceosome assembly in yeast extracts.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • pre-mRNA branch point binding Source: SGD

GO - Biological processi

  • mRNA branch site recognition Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor MUD2
Gene namesi
Name:MUD2
Ordered Locus Names:YKL074C
ORF Names:YKL358
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL074C.
SGDiS000001557. MUD2.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816541 – 527Splicing factor MUD2Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36084.
PRIDEiP36084.

PTM databases

iPTMnetiP36084.

Interactioni

Subunit structurei

MSL5, MUD2 and PRP40 interact to form the commitment complex 2 (CC2), a precursor of mature spliceosomes.

Binary interactionsi

WithEntry#Exp.IntActNotes
MSL5Q121865EBI-11612,EBI-34012

Protein-protein interaction databases

BioGridi34057. 161 interactors.
DIPiDIP-758N.
IntActiP36084. 11 interactors.
MINTiMINT-2786506.

Structurei

3D structure databases

ProteinModelPortaliP36084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini424 – 511RRMAdd BLAST88

Sequence similaritiesi

Phylogenomic databases

InParanoidiP36084.
KOiK12837.
OMAiDYRINID.
OrthoDBiEOG092C1ST8.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR003954. RRM_dom_euk.
[Graphical view]
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequencei

Sequence statusi: Complete.

P36084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEKRLEDL RSKIMESIGK SEKDVVPIEN KRFNTDNAVI DTHFKRQKSD
60 70 80 90 100
GELPKAPKSR NVSHSNNRGP SSIITMSTNR TTYEQTRAGP HRQSYRDASG
110 120 130 140 150
RSYNRENRYS SHNTGPQWNN NPYNRQRDER RGRNERFDRR GRNGNGNYDR
160 170 180 190 200
FNYQRKNEGS KFNGDRDKRQ LQTNKYDMNY NSQNVMYPGS SFDSPAYYNM
210 220 230 240 250
ASSKANSRLV ISGLSQSSDP SIVARLKDLL ENFISGLQKT ESNAEDFKIS
260 270 280 290 300
NFYIGEGRPD HIIVEFSSQI CSTMVLACRS FFNAKLGTFD LKWRRPNDYV
310 320 330 340 350
QQLDHLVDFC RGTVIALENL ENIGEGEDYR MKELFSSLNV TNGTAKPLFY
360 370 380 390 400
KCSSNTNNTG KESEFTKCIL LSFEVVTQDI LDKLKPYKWF KPNDGKISQV
410 420 430 440 450
TSWITFQSLP NLVTQSVRVE SRVLLLLNCL DPLDLKDETF ITEIKETLKY
460 470 480 490 500
SIAGADTIKI CQPGVDYRLN FENLASGAGN IYIKFKTLEA AKHAMEELPG
510 520
TQFNDRTVLC TYIDEDDFDM MEATQLS
Length:527
Mass (Da):60,460
Last modified:August 16, 2004 - v3
Checksum:i884427AB57A12E12
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98A → R in AAA64215 (PubMed:7926772).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22449 Genomic DNA. Translation: AAA64215.1.
X75780 Genomic DNA. Translation: CAA53400.1.
Z28074 Genomic DNA. Translation: CAA81911.1.
BK006944 Genomic DNA. Translation: DAA09082.1.
PIRiS37899.
RefSeqiNP_012849.1. NM_001179640.1.

Genome annotation databases

EnsemblFungiiYKL074C; YKL074C; YKL074C.
GeneIDi853788.
KEGGisce:YKL074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22449 Genomic DNA. Translation: AAA64215.1.
X75780 Genomic DNA. Translation: CAA53400.1.
Z28074 Genomic DNA. Translation: CAA81911.1.
BK006944 Genomic DNA. Translation: DAA09082.1.
PIRiS37899.
RefSeqiNP_012849.1. NM_001179640.1.

3D structure databases

ProteinModelPortaliP36084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34057. 161 interactors.
DIPiDIP-758N.
IntActiP36084. 11 interactors.
MINTiMINT-2786506.

PTM databases

iPTMnetiP36084.

Proteomic databases

MaxQBiP36084.
PRIDEiP36084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL074C; YKL074C; YKL074C.
GeneIDi853788.
KEGGisce:YKL074C.

Organism-specific databases

EuPathDBiFungiDB:YKL074C.
SGDiS000001557. MUD2.

Phylogenomic databases

InParanoidiP36084.
KOiK12837.
OMAiDYRINID.
OrthoDBiEOG092C1ST8.

Enzyme and pathway databases

BioCyciYEAST:G3O-31870-MONOMER.

Miscellaneous databases

PROiP36084.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR003954. RRM_dom_euk.
[Graphical view]
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMUD2_YEAST
AccessioniPrimary (citable) accession number: P36084
Secondary accession number(s): D6VXL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.