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Protein

Helper of Tim protein 13

Gene

HOT13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the assembly or recycling of the small Tim proteins in the mitochondrial intermembrane, thereby participating in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Probably acts by facilitating the formation of disulfide bonds in small Tim proteins.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 94CHY-typePROSITE-ProRule annotationAdd BLAST85

GO - Molecular functioni

  • zinc ion binding Source: SGD

GO - Biological processi

  • protein import into mitochondrial intermembrane space Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31878-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Helper of Tim protein 13
Gene namesi
Name:HOT13
Ordered Locus Names:YKL084W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL084W.
SGDiS000001567. HOT13.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: SGD
  • mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002031651 – 116Helper of Tim protein 13Add BLAST116

Proteomic databases

MaxQBiP36078.
PRIDEiP36078.

Interactioni

Subunit structurei

Interacts with the small Tim proteins TIM8, TIM9, TIM10, TIM12, and TIM13.

Protein-protein interaction databases

BioGridi34049. 3 interactors.
IntActiP36078. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP36078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 CHY-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 94CHY-typePROSITE-ProRule annotationAdd BLAST85

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000235148.
InParanoidiP36078.
OMAiQTRCIHY.
OrthoDBiEOG092C5PHC.

Family and domain databases

InterProiIPR016694. UCP017292.
IPR008913. Znf_CHY.
[Graphical view]
PfamiPF05495. zf-CHY. 1 hit.
[Graphical view]
PIRSFiPIRSF017292. UCP017292_Znf_CHY. 1 hit.
PROSITEiPS51266. ZF_CHY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETAIYGKT VDDQSRCVHW HLPKDVIAIR FKCCDKYYAC FECHQELSSH
60 70 80 90 100
PLEKYDLLDD ANKHLIICGV CRHEMTFAEY YDYNSNLICP NCRSPFNPGC
110
KLHYHLYFQN PPPAMC
Length:116
Mass (Da):13,641
Last modified:June 1, 1994 - v1
Checksum:i57464E5FD9A591F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71C → R in AAS56591 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28084 Genomic DNA. Translation: CAA81922.1.
AY558265 Genomic DNA. Translation: AAS56591.1.
BK006944 Genomic DNA. Translation: DAA09074.1.
PIRiS37909.
RefSeqiNP_012839.1. NM_001179650.1.

Genome annotation databases

EnsemblFungiiYKL084W; YKL084W; YKL084W.
GeneIDi853778.
KEGGisce:YKL084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28084 Genomic DNA. Translation: CAA81922.1.
AY558265 Genomic DNA. Translation: AAS56591.1.
BK006944 Genomic DNA. Translation: DAA09074.1.
PIRiS37909.
RefSeqiNP_012839.1. NM_001179650.1.

3D structure databases

ProteinModelPortaliP36078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34049. 3 interactors.
IntActiP36078. 2 interactors.

Proteomic databases

MaxQBiP36078.
PRIDEiP36078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL084W; YKL084W; YKL084W.
GeneIDi853778.
KEGGisce:YKL084W.

Organism-specific databases

EuPathDBiFungiDB:YKL084W.
SGDiS000001567. HOT13.

Phylogenomic databases

HOGENOMiHOG000235148.
InParanoidiP36078.
OMAiQTRCIHY.
OrthoDBiEOG092C5PHC.

Enzyme and pathway databases

BioCyciYEAST:G3O-31878-MONOMER.

Miscellaneous databases

PROiP36078.

Family and domain databases

InterProiIPR016694. UCP017292.
IPR008913. Znf_CHY.
[Graphical view]
PfamiPF05495. zf-CHY. 1 hit.
[Graphical view]
PIRSFiPIRSF017292. UCP017292_Znf_CHY. 1 hit.
PROSITEiPS51266. ZF_CHY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOT13_YEAST
AccessioniPrimary (citable) accession number: P36078
Secondary accession number(s): D6VXK4, Q6Q598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.