P36078 (HOT13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Helper of Tim protein 13

Gene names

Name:	HOT13
Ordered Locus Names:	YKL084W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the assembly or recycling of the small Tim proteins in the mitochondrial intermembrane, thereby participating in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Probably acts by facilitating the formation of disulfide bonds in small Tim proteins. Ref.4
Subunit structure	Interacts with the small Tim proteins TIM8, TIM9, TIM10, TIM12, and TIM13.
Subcellular location	Mitochondrion intermembrane space. Mitochondrion membrane; Peripheral membrane protein Ref.4.
Sequence similarities	Contains 1 CHY-type zinc finger.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial intermembrane space Inferred from physical interaction Ref.4. Source: SGD
Cellular_component	mitochondrial intermembrane space Inferred from direct assay Ref.4. Source: SGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from direct assay PubMed 18787558. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	Helper of Tim protein 13		PRO_0000203165
Regions
	Zinc finger	10 – 94	85	CHY-type
Experimental info
	Sequence conflict	71	1	C → R in AAS56591. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P36078 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 57464E5FD9A591F8	FASTA	116	13,641
	10 20 30 40 50 60 MIETAIYGKT VDDQSRCVHW HLPKDVIAIR FKCCDKYYAC FECHQELSSH PLEKYDLLDD 70 80 90 100 110 ANKHLIICGV CRHEMTFAEY YDYNSNLICP NCRSPFNPGC KLHYHLYFQN PPPAMC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The role of Hot13p and redox chemistry in the mitochondrial TIM22 import pathway." Curran S.P., Leuenberger D., Leverich E.P., Hwang D.K., Beverly K.N., Koehler C.M. J. Biol. Chem. 279:43744-43751(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28084 Genomic DNA. Translation: CAA81922.1. AY558265 Genomic DNA. Translation: AAS56591.1. BK006944 Genomic DNA. Translation: DAA09074.1.
PIR	S37909.
RefSeq	NP_012839.1. NM_001179650.1.
3D structure databases
ProteinModelPortal	P36078.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36078. 1 interaction.
STRING	4932.YKL084W.
Proteomic databases
PaxDb	P36078.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL084W; YKL084W; YKL084W.
GeneID	853778.
KEGG	sce:YKL084W.
Organism-specific databases
CYGD	YKL084w.
SGD	S000001567. HOT13.
Phylogenomic databases
eggNOG	COG4357.
HOGENOM	HOG000235148.
OMA	QTRCIHY.
OrthoDB	EOG4C2MMB.
Gene expression databases
Genevestigator	P36078.
GermOnline	YKL084W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016694. UCP017292_Znf_CHY. IPR008913. Znf_CHY. [Graphical view]
Pfam	PF05495. zf-CHY. 1 hit. [Graphical view]
PIRSF	PIRSF017292. UCP017292_Znf_CHY. 1 hit.
PROSITE	PS51266. ZF_CHY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974891.

Entry information

Entry name

HOT13_YEAST

Accession

Primary (citable) accession number: P36078
Secondary accession number(s): D6VXK4, Q6Q598

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program