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Protein

Helper of Tim protein 13

Gene

HOT13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the assembly or recycling of the small Tim proteins in the mitochondrial intermembrane, thereby participating in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Probably acts by facilitating the formation of disulfide bonds in small Tim proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 9485CHY-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • zinc ion binding Source: SGD

GO - Biological processi

  • protein import into mitochondrial intermembrane space Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31878-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Helper of Tim protein 13
Gene namesi
Name:HOT13
Ordered Locus Names:YKL084W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKL084w.
EuPathDBiFungiDB:YKL084W.
SGDiS000001567. HOT13.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: SGD
  • mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Helper of Tim protein 13PRO_0000203165Add
BLAST

Proteomic databases

MaxQBiP36078.
PaxDbiP36078.

Expressioni

Gene expression databases

GenevestigatoriP36078.

Interactioni

Subunit structurei

Interacts with the small Tim proteins TIM8, TIM9, TIM10, TIM12, and TIM13.

Protein-protein interaction databases

BioGridi34049. 3 interactions.
IntActiP36078. 2 interactions.
STRINGi4932.YKL084W.

Structurei

3D structure databases

ProteinModelPortaliP36078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 CHY-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 9485CHY-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG4357.
HOGENOMiHOG000235148.
InParanoidiP36078.
OMAiQTRCIHY.
OrthoDBiEOG7V4BBM.

Family and domain databases

InterProiIPR016694. UCP017292.
IPR008913. Znf_CHY.
[Graphical view]
PfamiPF05495. zf-CHY. 1 hit.
[Graphical view]
PIRSFiPIRSF017292. UCP017292_Znf_CHY. 1 hit.
PROSITEiPS51266. ZF_CHY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETAIYGKT VDDQSRCVHW HLPKDVIAIR FKCCDKYYAC FECHQELSSH
60 70 80 90 100
PLEKYDLLDD ANKHLIICGV CRHEMTFAEY YDYNSNLICP NCRSPFNPGC
110
KLHYHLYFQN PPPAMC
Length:116
Mass (Da):13,641
Last modified:June 1, 1994 - v1
Checksum:i57464E5FD9A591F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711C → R in AAS56591 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28084 Genomic DNA. Translation: CAA81922.1.
AY558265 Genomic DNA. Translation: AAS56591.1.
BK006944 Genomic DNA. Translation: DAA09074.1.
PIRiS37909.
RefSeqiNP_012839.1. NM_001179650.1.

Genome annotation databases

EnsemblFungiiYKL084W; YKL084W; YKL084W.
GeneIDi853778.
KEGGisce:YKL084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28084 Genomic DNA. Translation: CAA81922.1.
AY558265 Genomic DNA. Translation: AAS56591.1.
BK006944 Genomic DNA. Translation: DAA09074.1.
PIRiS37909.
RefSeqiNP_012839.1. NM_001179650.1.

3D structure databases

ProteinModelPortaliP36078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34049. 3 interactions.
IntActiP36078. 2 interactions.
STRINGi4932.YKL084W.

Proteomic databases

MaxQBiP36078.
PaxDbiP36078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL084W; YKL084W; YKL084W.
GeneIDi853778.
KEGGisce:YKL084W.

Organism-specific databases

CYGDiYKL084w.
EuPathDBiFungiDB:YKL084W.
SGDiS000001567. HOT13.

Phylogenomic databases

eggNOGiCOG4357.
HOGENOMiHOG000235148.
InParanoidiP36078.
OMAiQTRCIHY.
OrthoDBiEOG7V4BBM.

Enzyme and pathway databases

BioCyciYEAST:G3O-31878-MONOMER.

Miscellaneous databases

NextBioi974891.
PROiP36078.

Gene expression databases

GenevestigatoriP36078.

Family and domain databases

InterProiIPR016694. UCP017292.
IPR008913. Znf_CHY.
[Graphical view]
PfamiPF05495. zf-CHY. 1 hit.
[Graphical view]
PIRSFiPIRSF017292. UCP017292_Znf_CHY. 1 hit.
PROSITEiPS51266. ZF_CHY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The role of Hot13p and redox chemistry in the mitochondrial TIM22 import pathway."
    Curran S.P., Leuenberger D., Leverich E.P., Hwang D.K., Beverly K.N., Koehler C.M.
    J. Biol. Chem. 279:43744-43751(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHOT13_YEAST
AccessioniPrimary (citable) accession number: P36078
Secondary accession number(s): D6VXK4, Q6Q598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 29, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.