Reviewed, UniProtKB/Swiss-Prot P36077 (SRX1_YEAST)

Names and origin

Protein names

Recommended name:
    Sulfiredoxin
    EC=1.8.98.2
Alternative name(s):
    Sulphiredoxin

Gene names

Name:	SRX1
Ordered Locus Names:	YKL086W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxin TSA1. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and as thioltransferase. Ref.5
Catalytic activity	Peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH = peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R.
Cofactor	Magnesium.
Subcellular location	Cytoplasm. Nucleus. Ref.3
Induction	By hydrogen peroxide. Ref.5
Post-translational modification	Forms a transient disulfide bond with TSA1 during the reduction of cysteine sulfinic acid (-SO2H).
Miscellaneous	Present with 538 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the sulfiredoxin family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Antioxidant Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro antioxidant activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct sulfiredoxin activity Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSU81	P40073	1	EBI-26774,EBI-18140

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 127	127	Sulfiredoxin		PRO_0000211435
Amino acid modifications
	Disulfide bond	84		Interchain (with C-48 in TSA1); transient Ref.5
Experimental info
	Mutagenesis	48	1	C → S: Minor effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid. Ref.5
	Mutagenesis	84	1	C → S: Abolishes formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid. Ref.5
	Mutagenesis	106	1	C → S: No effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P36077-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: AC15AFD2F6CBC9A0	FASTA	127	13,854
	10 20 30 40 50 60 MSLQSNSVKP TEIPLSEIRR PLAPVLDPQK IDAMVATMKG IPTASKTCSL EQAEAAASAG 70 80 90 100 110 120 ELPPVDVLGV RVKGQTLYYA FGGCHRLQAY DRRARETQNA AFPVRCRVLP ATPRQIRMYL GSSLDIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin." Biteau B., Labarre J., Toledano M.B. Nature 425:980-984(2003) [PubMed: 14586471] [Abstract] Cited for: FUNCTION, INDUCTION, DISULFIDE BOND WITH TSA1, MUTAGENESIS OF CYS-48; CYS-84 AND CYS-106.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28086 Genomic DNA. Translation: CAA81924.1. AY558350 Genomic DNA. Translation: AAS56676.1.
PIR	S37911.
RefSeq	NP_012837.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4344N.
IntAct	P36077. 6 interactions.
Genome annotation databases
Ensembl	YKL086W. Saccharomyces cerevisiae. [Contig view]
GeneID	853776.
GenomeReviews	Gene locus YKL086W in contig Y13137_GR.
KEGG	sce:YKL086W.
NMPDR	fig|4932.3.peg.3821.
Organism-specific databases
CYGD	YKL086w.
SGD	S000001569. SRX1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P36077.
OMA	P36077. CHRLQAY.
Enzyme and pathway databases
BRENDA	1.8.98.2. 250.
Gene expression databases
GermOnline	YKL086W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003115. ParBc. IPR016692. Sulfiredoxin. [Graphical view]
Pfam	PF02195. ParBc. 1 hit. [Graphical view]
PIRSF	PIRSF017267. Sulfiredoxin. 1 hit.
SMART	SM00470. ParB. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974885.

Entry information

Entry name

SRX1_YEAST

Accession

Primary (citable) accession number: P36077

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)