P36077 (SRX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfiredoxin
EC=1.8.98.2

Alternative name(s):
Sulphiredoxin

Gene names

Name:	SRX1
Ordered Locus Names:	YKL086W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxin TSA1. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and as thioltransferase. Ref.6
Catalytic activity	Peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH = peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R.
Cofactor	Magnesium.
Subcellular location	Cytoplasm. Nucleus Ref.4.
Induction	By hydrogen peroxide. Ref.6
Post-translational modification	Forms a transient disulfide bond with TSA1 during the reduction of cysteine sulfinic acid (-SO2H).
Miscellaneous	Present with 538 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the sulfiredoxin family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Antioxidant Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to oxidative stress Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro sulfiredoxin activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSU81	P40073	1	EBI-26774,EBI-18140

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 127	127	Sulfiredoxin		PRO_0000211435
Amino acid modifications
	Disulfide bond	84		Interchain (with C-48 in TSA1); transient Ref.6
Experimental info
	Mutagenesis	48	1	C → S: Minor effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid. Ref.6
	Mutagenesis	84	1	C → S: Abolishes formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid. Ref.6
	Mutagenesis	106	1	C → S: No effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P36077 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: AC15AFD2F6CBC9A0	FASTA	127	13,854
	10 20 30 40 50 60 MSLQSNSVKP TEIPLSEIRR PLAPVLDPQK IDAMVATMKG IPTASKTCSL EQAEAAASAG 70 80 90 100 110 120 ELPPVDVLGV RVKGQTLYYA FGGCHRLQAY DRRARETQNA AFPVRCRVLP ATPRQIRMYL GSSLDIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin." Biteau B., Labarre J., Toledano M.B. Nature 425:980-984(2003) [PubMed: 14586471] [Abstract] Cited for: FUNCTION, INDUCTION, DISULFIDE BOND WITH TSA1, MUTAGENESIS OF CYS-48; CYS-84 AND CYS-106.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28086 Genomic DNA. Translation: CAA81924.1. AY558350 Genomic DNA. Translation: AAS56676.1. BK006944 Genomic DNA. Translation: DAA09072.1.
PIR	S37911.
RefSeq	NP_012837.1. NM_001179652.1.
3D structure databases
ProteinModelPortal	P36077.
SMR	P36077. Positions 2-126.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4344N.
IntAct	P36077. 2 interactions.
MINT	MINT-537334.
STRING	P36077.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL086W; YKL086W; YKL086W.
GeneID	853776.
KEGG	sce:YKL086W.
NMPDR	fig|4932.3.peg.3821.
Organism-specific databases
CYGD	YKL086w.
SGD	S000001569. SRX1.
Phylogenomic databases
eggNOG	fuNOG10980.
GeneTree	EFGT00050000008637.
HOGENOM	HBG599373.
OMA	CHRLQAY.
OrthoDB	EOG4PVS87.
Gene expression databases
ArrayExpress	P36077.
Genevestigator	P36077.
GermOnline	YKL086W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003115. ParBc. IPR016692. Sulfiredoxin. [Graphical view]
KO	K12260.
PANTHER	PTHR21348. PTHR21348. 1 hit.
Pfam	PF02195. ParBc. 1 hit. [Graphical view]
PIRSF	PIRSF017267. Sulfiredoxin. 1 hit.
SMART	SM00470. ParB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974885.

Entry information

Entry name

SRX1_YEAST

Accession

Primary (citable) accession number: P36077
Secondary accession number(s): D6VXK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program