Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P36076 (CAB3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Coenzyme A biosynthesis protein 3
Gene names
Name:CAB3
Ordered Locus Names:YKL088W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the phosphopantothenoylcysteine decarboxylase (PPCDC) involved in the coenzyme A synthesis. Ref.8 Ref.9

Subunit structure

Component of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex, a heterotrimer composed of CAB3, HAL3 and VHS3.

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Coenzyme A biosynthesis protein 3
PRO_0000182039

Regions

Compositional bias508 – 57063Asp/Glu-rich (highly acidic)

Amino acid modifications

Modified residue421Phosphoserine Ref.5 Ref.6 Ref.7
Modified residue1161Phosphoserine Ref.5 Ref.10
Modified residue1211Phosphoserine Ref.5 Ref.10
Modified residue1241Phosphoserine Ref.5
Modified residue2641Phosphoserine Ref.7

Experimental info

Mutagenesis4781C → S: Abolishes PPCDC activity. Ref.9

Sequences

Sequence LengthMass (Da)Tools
P36076 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 9C674C2394EFCEAB

FASTA57165,238
        10         20         30         40         50         60 
MTDEKVNSDQ NMNGKQGVNL ISSLPTTQVP VSILTNKERR KSIHDESNFE RSDSHEDQSK 

        70         80         90        100        110        120 
SNSNRRNIYK NDYSTNLRDF SFANLKQNSE RNKDGHEIQI NTSMPANTNG QQKRFSPSLP 

       130        140        150        160        170        180 
SAVSFTVPEV ERLPYHRYSI SNKPGKQQQQ QEQLQQNQQQ EEQQKAQLQE QNQRAKQQEE 

       190        200        210        220        230        240 
VKQIQEQVQK KQTERQQLID EKERIANAIF KENTTNDGTD IRKHSVSSGT SNSEDEVDSP 

       250        260        270        280        290        300 
SMEKNSIVHM PGDFIYFNPK SNASKPITAK AAPLSANNST HKNKEVITAP TGPRVPFTEF 

       310        320        330        340        350        360 
FQKEDDKKFH ILIGATGSVA TIKVPLIIDK LFKIYGPEKI SIQLIVTKPA EHFLKGLKMS 

       370        380        390        400        410        420 
THVKIWREED AWVFDAVNKN DTSLSLNLIL HHELRKWADI FLIAPLSANT LAKLANGICN 

       430        440        450        460        470        480 
NLLTSVMRDW SPLTPVLIAP AMNTFMYINP MTKKHLTSLV QDYPFIQVLK PVEKVLICGD 

       490        500        510        520        530        540 
IGMGGMREWT DIVEIVRRRI NEIRKARDEE TGDKEQEQEE QEGADNEDDD DEDDEEDEED 

       550        560        570 
EEEEEALNET ASDESNDEED EEDEEDVKTE V 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-116; SER-121 AND SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces cerevisiae: identification of a conditional mutation in the pantothenate kinase gene CAB1."
Olzhausen J., Schuebbe S., Schueller H.-J.
Curr. Genet. 55:163-173(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis."
Ruiz A., Gonzalez A., Munoz I., Serrano R., Abrie J.A., Strauss E., Arino J.
Nat. Chem. Biol. 5:920-928(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-478, INTERACTION WITH HAL3 AND VHS3.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116 AND SER-121, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28088 Genomic DNA. Translation: CAA81926.1.
BK006944 Genomic DNA. Translation: DAA09070.1.
PIRS37913.
RefSeqNP_012835.1. NM_001179654.1.

3D structure databases

ProteinModelPortalP36076.
SMRP36076. Positions 307-497.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34045. 28 interactions.
DIPDIP-4475N.
IntActP36076. 67 interactions.
MINTMINT-541287.
STRING4932.YKL088W.

Proteomic databases

MaxQBP36076.
PaxDbP36076.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL088W; YKL088W; YKL088W.
GeneID853774.
KEGGsce:YKL088W.

Organism-specific databases

CYGDYKL088w.
SGDS000001571. CAB3.

Phylogenomic databases

eggNOGCOG0452.
GeneTreeENSGT00440000038107.
KOK01598.
OMAISIQLIV.
OrthoDBEOG7N906N.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER3O-299.
YEAST:MONOMER3O-299.

Gene expression databases

GenevestigatorP36076.

Family and domain databases

Gene3D3.40.50.1950. 1 hit.
InterProIPR003382. Flavoprotein.
[Graphical view]
PfamPF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMSSF52507. SSF52507. 1 hit.
ProtoNetSearch...

Other

NextBio974879.

Entry information

Entry nameCAB3_YEAST
AccessionPrimary (citable) accession number: P36076
Secondary accession number(s): D6VXK0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families