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P36076

- CAB3_YEAST

UniProt

P36076 - CAB3_YEAST

Protein

Coenzyme A biosynthesis protein 3

Gene

CAB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Component of the phosphopantothenoylcysteine decarboxylase (PPCDC) involved in the coenzyme A synthesis.2 Publications

    GO - Molecular functioni

    1. phosphopantothenoylcysteine decarboxylase activity Source: SGD
    2. protein binding Source: IntAct
    3. purine nucleotide binding Source: SGD

    GO - Biological processi

    1. coenzyme A biosynthetic process Source: SGD
    2. response to salt stress Source: SGD

    Keywords - Biological processi

    Coenzyme A biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-299.
    YEAST:MONOMER3O-299.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme A biosynthesis protein 3
    Gene namesi
    Name:CAB3
    Ordered Locus Names:YKL088W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL088w.
    SGDiS000001571. CAB3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. phosphopantothenoylcysteine decarboxylase complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi478 – 4781C → S: Abolishes PPCDC activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 571571Coenzyme A biosynthesis protein 3PRO_0000182039Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421Phosphoserine3 Publications
    Modified residuei116 – 1161Phosphoserine2 Publications
    Modified residuei121 – 1211Phosphoserine2 Publications
    Modified residuei124 – 1241Phosphoserine1 Publication
    Modified residuei264 – 2641Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36076.
    PaxDbiP36076.

    Expressioni

    Gene expression databases

    GenevestigatoriP36076.

    Interactioni

    Subunit structurei

    Component of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex, a heterotrimer composed of CAB3, HAL3 and VHS3.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PPZ1P265706EBI-26778,EBI-13807
    PPZ2P333294EBI-26778,EBI-13815
    SIS2P360243EBI-26778,EBI-17250
    VHS3Q084384EBI-26778,EBI-30482

    Protein-protein interaction databases

    BioGridi34045. 28 interactions.
    DIPiDIP-4475N.
    IntActiP36076. 67 interactions.
    MINTiMINT-541287.
    STRINGi4932.YKL088W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36076.
    SMRiP36076. Positions 307-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi508 – 57063Asp/Glu-rich (highly acidic)Add
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0452.
    GeneTreeiENSGT00440000038107.
    KOiK01598.
    OMAiISIQLIV.
    OrthoDBiEOG7N906N.

    Family and domain databases

    Gene3Di3.40.50.1950. 1 hit.
    InterProiIPR003382. Flavoprotein.
    [Graphical view]
    PfamiPF02441. Flavoprotein. 1 hit.
    [Graphical view]
    SUPFAMiSSF52507. SSF52507. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36076-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDEKVNSDQ NMNGKQGVNL ISSLPTTQVP VSILTNKERR KSIHDESNFE    50
    RSDSHEDQSK SNSNRRNIYK NDYSTNLRDF SFANLKQNSE RNKDGHEIQI 100
    NTSMPANTNG QQKRFSPSLP SAVSFTVPEV ERLPYHRYSI SNKPGKQQQQ 150
    QEQLQQNQQQ EEQQKAQLQE QNQRAKQQEE VKQIQEQVQK KQTERQQLID 200
    EKERIANAIF KENTTNDGTD IRKHSVSSGT SNSEDEVDSP SMEKNSIVHM 250
    PGDFIYFNPK SNASKPITAK AAPLSANNST HKNKEVITAP TGPRVPFTEF 300
    FQKEDDKKFH ILIGATGSVA TIKVPLIIDK LFKIYGPEKI SIQLIVTKPA 350
    EHFLKGLKMS THVKIWREED AWVFDAVNKN DTSLSLNLIL HHELRKWADI 400
    FLIAPLSANT LAKLANGICN NLLTSVMRDW SPLTPVLIAP AMNTFMYINP 450
    MTKKHLTSLV QDYPFIQVLK PVEKVLICGD IGMGGMREWT DIVEIVRRRI 500
    NEIRKARDEE TGDKEQEQEE QEGADNEDDD DEDDEEDEED EEEEEALNET 550
    ASDESNDEED EEDEEDVKTE V 571
    Length:571
    Mass (Da):65,238
    Last modified:June 1, 1994 - v1
    Checksum:i9C674C2394EFCEAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28088 Genomic DNA. Translation: CAA81926.1.
    BK006944 Genomic DNA. Translation: DAA09070.1.
    PIRiS37913.
    RefSeqiNP_012835.1. NM_001179654.1.

    Genome annotation databases

    EnsemblFungiiYKL088W; YKL088W; YKL088W.
    GeneIDi853774.
    KEGGisce:YKL088W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28088 Genomic DNA. Translation: CAA81926.1 .
    BK006944 Genomic DNA. Translation: DAA09070.1 .
    PIRi S37913.
    RefSeqi NP_012835.1. NM_001179654.1.

    3D structure databases

    ProteinModelPortali P36076.
    SMRi P36076. Positions 307-497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34045. 28 interactions.
    DIPi DIP-4475N.
    IntActi P36076. 67 interactions.
    MINTi MINT-541287.
    STRINGi 4932.YKL088W.

    Proteomic databases

    MaxQBi P36076.
    PaxDbi P36076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL088W ; YKL088W ; YKL088W .
    GeneIDi 853774.
    KEGGi sce:YKL088W.

    Organism-specific databases

    CYGDi YKL088w.
    SGDi S000001571. CAB3.

    Phylogenomic databases

    eggNOGi COG0452.
    GeneTreei ENSGT00440000038107.
    KOi K01598.
    OMAi ISIQLIV.
    OrthoDBi EOG7N906N.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER3O-299.
    YEAST:MONOMER3O-299.

    Miscellaneous databases

    NextBioi 974879.

    Gene expression databases

    Genevestigatori P36076.

    Family and domain databases

    Gene3Di 3.40.50.1950. 1 hit.
    InterProi IPR003382. Flavoprotein.
    [Graphical view ]
    Pfami PF02441. Flavoprotein. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52507. SSF52507. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-116; SER-121 AND SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces cerevisiae: identification of a conditional mutation in the pantothenate kinase gene CAB1."
      Olzhausen J., Schuebbe S., Schueller H.-J.
      Curr. Genet. 55:163-173(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis."
      Ruiz A., Gonzalez A., Munoz I., Serrano R., Abrie J.A., Strauss E., Arino J.
      Nat. Chem. Biol. 5:920-928(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-478, INTERACTION WITH HAL3 AND VHS3.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116 AND SER-121, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCAB3_YEAST
    AccessioniPrimary (citable) accession number: P36076
    Secondary accession number(s): D6VXK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2940 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3