##gff-version 3
P36075	UniProtKB	Chain	1	443	.	.	.	ID=PRO_0000079558;Note=Endonuclease CUE2	
P36075	UniProtKB	Domain	8	51	.	.	.	Note=CUE 1;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00468	
P36075	UniProtKB	Domain	55	98	.	.	.	Note=CUE 2;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00468	
P36075	UniProtKB	Domain	347	443	.	.	.	Note=Smr;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00321	
P36075	UniProtKB	Mutagenesis	20	21	.	.	.	Note=In CUE-D1 mutant%3B impaired ability to bind polyubiquitinated RPS7/eS7%3B when associated with 46-A-A-47. FP->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36583309;Dbxref=PMID:36583309	
P36075	UniProtKB	Mutagenesis	46	47	.	.	.	Note=In CUE-D1 mutant%3B impaired ability to bind polyubiquitinated RPS7/eS7%3B when associated with 21-A-A-22. LL->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36583309;Dbxref=PMID:36583309	
P36075	UniProtKB	Mutagenesis	67	68	.	.	.	Note=In CUE-D2 mutant%3B impaired ability to bind polyubiquitinated RPS7/eS7%3B when associated with 93-A-A-94. FP->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36583309;Dbxref=PMID:36583309	
P36075	UniProtKB	Mutagenesis	93	94	.	.	.	Note=In CUE-D2 mutant%3B impaired ability to bind polyubiquitinated RPS7/eS7%3B when associated with 66-A-A-67. LL->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36583309;Dbxref=PMID:36583309	
P36075	UniProtKB	Mutagenesis	122	122	.	.	.	Note=Abolished ability to act downstream of the RQT (ribosome quality control trigger) complex. W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36583309;Dbxref=PMID:36583309	
P36075	UniProtKB	Mutagenesis	348	350	.	.	.	Note=Abolished mRNA endonuclease activity. DFH->AAA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31219035;Dbxref=PMID:31219035	
P36075	UniProtKB	Mutagenesis	402	402	.	.	.	Note=Decreased ability to participate to No-Go Decay (NGD) pathway in response to ribosome collision. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31219035;Dbxref=PMID:31219035	
P36075	UniProtKB	Mutagenesis	402	402	.	.	.	Note=No effect. R->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31219035;Dbxref=PMID:31219035	
P36075	UniProtKB	Helix	9	19	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1OTR	
P36075	UniProtKB	Beta strand	21	23	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1OTR	
P36075	UniProtKB	Helix	25	34	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1OTR	
P36075	UniProtKB	Turn	35	37	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1OTR	
P36075	UniProtKB	Helix	40	49	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1OTR