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Protein

RNA polymerase I-specific transcription initiation factor RRN3

Gene

RRN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient transcription initiation by RNA polymerase I. Interacts with Pol I in the absence of template DNA and stimulates recruitment of Pol I, but does not remain as part of stable preinitiation complex.

GO - Molecular functioni

  • rDNA binding Source: SGD
  • RNA polymerase I core binding Source: SGD
  • RNA polymerase I transcription factor binding Source: SGD
  • transcription factor activity, core RNA polymerase I binding Source: SGD
  • transcription factor activity, RNA polymerase I transcription factor binding Source: SGD

GO - Biological processi

  • transcription initiation from RNA polymerase I promoter for nuclear large rRNA transcript Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31907-MONOMER.
ReactomeiR-SCE-73762. RNA Polymerase I Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase I-specific transcription initiation factor RRN3
Gene namesi
Name:RRN3
Ordered Locus Names:YKL125W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL125W.
SGDiS000001608. RRN3.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114301 – 627RNA polymerase I-specific transcription initiation factor RRN3Add BLAST627

Proteomic databases

MaxQBiP36070.
PRIDEiP36070.

Interactioni

Subunit structurei

Monomer (Probable). Interacts with NOP53.Curated1 Publication

GO - Molecular functioni

  • RNA polymerase I core binding Source: SGD
  • RNA polymerase I transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi34011. 19 interactors.
DIPiDIP-6657N.
IntActiP36070. 7 interactors.
MINTiMINT-624762.

Structurei

Secondary structure

1627
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 64Combined sources16
Helixi69 – 79Combined sources11
Helixi90 – 102Combined sources13
Helixi103 – 107Combined sources5
Helixi110 – 112Combined sources3
Helixi113 – 120Combined sources8
Helixi125 – 127Combined sources3
Helixi130 – 146Combined sources17
Helixi148 – 150Combined sources3
Helixi151 – 160Combined sources10
Helixi161 – 163Combined sources3
Helixi166 – 182Combined sources17
Helixi184 – 186Combined sources3
Helixi187 – 196Combined sources10
Helixi205 – 221Combined sources17
Helixi223 – 225Combined sources3
Helixi226 – 251Combined sources26
Helixi323 – 325Combined sources3
Helixi326 – 346Combined sources21
Helixi349 – 353Combined sources5
Helixi357 – 370Combined sources14
Turni371 – 374Combined sources4
Helixi383 – 389Combined sources7
Helixi393 – 408Combined sources16
Beta strandi410 – 412Combined sources3
Helixi414 – 430Combined sources17
Helixi436 – 457Combined sources22
Helixi458 – 460Combined sources3
Beta strandi463 – 465Combined sources3
Helixi467 – 470Combined sources4
Helixi471 – 487Combined sources17
Helixi488 – 491Combined sources4
Helixi499 – 501Combined sources3
Helixi502 – 511Combined sources10
Helixi516 – 519Combined sources4
Helixi522 – 534Combined sources13
Helixi541 – 553Combined sources13
Helixi579 – 590Combined sources12
Helixi600 – 606Combined sources7
Helixi607 – 609Combined sources3
Helixi613 – 616Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TJ1X-ray2.85A/B1-627[»]
5G5Lelectron microscopy4.80O1-627[»]
ProteinModelPortaliP36070.
SMRiP36070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 259Poly-Asp8
Compositional biasi267 – 274Poly-Asp8
Compositional biasi277 – 280Poly-Asp4
Compositional biasi546 – 549Poly-Asn4

Sequence similaritiesi

Belongs to the RRN3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000001488.
HOGENOMiHOG000093739.
InParanoidiP36070.
KOiK15216.
OMAiCYIFCFR.
OrthoDBiEOG092C16U7.

Family and domain databases

InterProiIPR007991. RNA_pol_I_trans_ini_fac_RRN3.
[Graphical view]
PANTHERiPTHR12790. PTHR12790. 1 hit.
PfamiPF05327. RRN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAFENTSKR PPQDFVAPID QKKRKVQFSD STGLVTLQPE EIKDEVFSAA
60 70 80 90 100
MYSRFVKSAL DDLDKNDSTQ IGIIANQVAL PSKNPERIND KNLNILLDIL
110 120 130 140 150
SSNINRIESS RGTFLIQSII NFEKWWELPP HTLSKYIYFI KILCSSIPKW
160 170 180 190 200
WQDVSMILVS CFILPIKQTV CHHDMLKYFL RMIPSSMGFI DTYLAKFFPN
210 220 230 240 250
KNDTRRKLVN YTSNLLKLRG YCSELGFQIW SLLIEKIISI DVELQNELDE
260 270 280 290 300
LDDDVDDDDL EEVDLEDDDD LDDDSGDDDD ENCGNSNEEL RSGAADGSQS
310 320 330 340 350
DSEDMDIIEG MDGTEEYNVE LTQGIKELST KLDSILTLVS THVEEQVTPE
360 370 380 390 400
SLESGEGVGV FNTLTTLFKT HVLPTYYTRS IQYIMFHVSQ QQLELMDSFL
410 420 430 440 450
VTLIDISFAV NEAAEKKIKS LQYLGSYIAR AKKLSRTQII FVASYLTSWL
460 470 480 490 500
NRYVIEREEE VDQRGGMERF KHFYAAFQAL CYIFCFRHNI FRDTDGNWEC
510 520 530 540 550
ELDKFFQRMV ISKFNPLKFC NENVMLMFAR IAQQESVAYC FSIIENNNNE
560 570 580 590 600
RLRGIIGKAD SDKKENSAQA NTTSSSWSLA TRQQFIDLQS YFPYDPLFLK
610 620
NYKILMKEYY IEWSEASGEY ESDGSDD
Length:627
Mass (Da):72,388
Last modified:June 1, 1994 - v1
Checksum:iA31E7386A9873FDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71927 Genomic DNA. Translation: CAA96470.1.
Z28125 Genomic DNA. Translation: CAA81966.1.
BK006944 Genomic DNA. Translation: DAA09036.1.
PIRiS37954.
RefSeqiNP_012797.1. NM_001179691.1.

Genome annotation databases

EnsemblFungiiYKL125W; YKL125W; YKL125W.
GeneIDi853734.
KEGGisce:YKL125W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71927 Genomic DNA. Translation: CAA96470.1.
Z28125 Genomic DNA. Translation: CAA81966.1.
BK006944 Genomic DNA. Translation: DAA09036.1.
PIRiS37954.
RefSeqiNP_012797.1. NM_001179691.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TJ1X-ray2.85A/B1-627[»]
5G5Lelectron microscopy4.80O1-627[»]
ProteinModelPortaliP36070.
SMRiP36070.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34011. 19 interactors.
DIPiDIP-6657N.
IntActiP36070. 7 interactors.
MINTiMINT-624762.

Proteomic databases

MaxQBiP36070.
PRIDEiP36070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL125W; YKL125W; YKL125W.
GeneIDi853734.
KEGGisce:YKL125W.

Organism-specific databases

EuPathDBiFungiDB:YKL125W.
SGDiS000001608. RRN3.

Phylogenomic databases

GeneTreeiENSGT00390000001488.
HOGENOMiHOG000093739.
InParanoidiP36070.
KOiK15216.
OMAiCYIFCFR.
OrthoDBiEOG092C16U7.

Enzyme and pathway databases

BioCyciYEAST:G3O-31907-MONOMER.
ReactomeiR-SCE-73762. RNA Polymerase I Transcription Initiation.

Miscellaneous databases

PROiP36070.

Family and domain databases

InterProiIPR007991. RNA_pol_I_trans_ini_fac_RRN3.
[Graphical view]
PANTHERiPTHR12790. PTHR12790. 1 hit.
PfamiPF05327. RRN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRRN3_YEAST
AccessioniPrimary (citable) accession number: P36070
Secondary accession number(s): D6VX70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 138 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.