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P36068 (SHE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SWI5-dependent HO expression protein 2
Gene names
Name:SHE2
Ordered Locus Names:YKL130C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, encoding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20 Ref.22 Ref.23

Subunit structure

Homodimer and Homotetramer. Interacts with LOC1, MYO4, PUF6, SHE3 and with RNA pol II subunits RPO21, SPT4 and SPT5. Ref.7 Ref.8 Ref.9 Ref.14 Ref.20 Ref.21 Ref.22 Ref.23

Subcellular location

Cytoplasm. Nucleus. Nucleusnucleolus. Note: Shuttles between the nucleus and cytoplasm and is exported in an mRNA-dependent manner. Nucleolar transit is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins. The presence the nucleus is essential for PUF6 and LOC1 to bind the ASH1 mRNA. Ref.10 Ref.14 Ref.17 Ref.19 Ref.20

Miscellaneous

Present with 4070 molecules/cell in log phase SD medium. Ref.12

Sequence similarities

Belongs to the SHE2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MYO4P324924EBI-26866,EBI-11681
SHE3P382723EBI-26866,EBI-21600

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246SWI5-dependent HO expression protein 2
PRO_0000203151

Regions

Motif15 – 239Nuclear localization signal

Amino acid modifications

Modified residue1661Phosphoserine Ref.18

Experimental info

Mutagenesis361N → S: Prevents association with ASH1 and IST2 mRNAs and leads to their mislocalization. Ref.14
Mutagenesis431R → A: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14
Mutagenesis441R → A: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14
Mutagenesis491R → K: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14
Mutagenesis521R → A or K: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14
Mutagenesis631R → A or K: Prevents association with ASH1 and IST2 mRNAs and leads to their mislocalization. Ref.14
Mutagenesis681C → Y: Prevents dimerization and RNA-binding. Ref.23
Mutagenesis1201S → Y: Prevents dimerization and RNA-binding. Ref.23
Mutagenesis1301L → Y: Prevents tetramerization. Ref.21

Secondary structure

........................ 246
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36068 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 5514B374655EFDBB

FASTA24628,251
        10         20         30         40         50         60 
MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK 

        70         80         90        100        110        120 
KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS 

       130        140        150        160        170        180 
LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC 

       190        200        210        220        230        240 
ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK 


FGKLKN 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Localization of ASH1 mRNA particles in living yeast."
Bertrand E., Chartrand P., Schaefer M., Shenoy S.M., Singer R.H., Long R.M.
Mol. Cell 2:437-445(1998) [PubMed: 9809065] [Abstract]
Cited for: FUNCTION.
[5]"Localization and anchoring of mRNA in budding yeast."
Beach D.L., Salmon E.D., Bloom K.
Curr. Biol. 9:569-578(1999) [PubMed: 10359695] [Abstract]
Cited for: FUNCTION.
[6]"Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins."
Munchow S., Sauter C., Jansen R.P.
J. Cell Sci. 112:1511-1518(1999) [PubMed: 10212145] [Abstract]
Cited for: FUNCTION.
[7]"She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p."
Bohl F., Kruse C., Frank A., Ferring D., Jansen R.P.
EMBO J. 19:5514-5524(2000) [PubMed: 11032818] [Abstract]
Cited for: FUNCTION, RNA-BINDING, INTERACTION WITH SHE3 AND MYO4.
[8]"She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA."
Long R.M., Gu W., Lorimer E., Singer R.H., Chartrand P.
EMBO J. 19:6592-6601(2000) [PubMed: 11101531] [Abstract]
Cited for: FUNCTION, RNA-BINDING, INTERACTION WITH SHE3.
[9]"The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p."
Takizawa P.A., Vale R.D.
Proc. Natl. Acad. Sci. U.S.A. 97:5273-5278(2000) [PubMed: 10792032] [Abstract]
Cited for: RNA-BINDING, INTERACTION WITH MYO4.
[10]"Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p."
Kruse C., Jaedicke A., Beaudouin J., Bohl F., Ferring D., Guttler T., Ellenberg J., Jansen R.P.
J. Cell Biol. 159:971-982(2002) [PubMed: 12499354] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae."
Estrada P., Kim J., Coleman J., Walker L., Dunn B., Takizawa P., Novick P., Ferro-Novick S.
J. Cell Biol. 163:1255-1266(2003) [PubMed: 14691136] [Abstract]
Cited for: FUNCTION.
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis."
Shepard K.A., Gerber A.P., Jambhekar A., Takizawa P.A., Brown P.O., Herschlag D., DeRisi J.L., Vale R.D.
Proc. Natl. Acad. Sci. U.S.A. 100:11429-11434(2003) [PubMed: 13679573] [Abstract]
Cited for: FUNCTION.
[14]"RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex."
Gonsalvez G.B., Lehmann K.A., Ho D.K., Stanitsa E.S., Williamson J.R., Long R.M.
RNA 9:1383-1399(2003) [PubMed: 14561888] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, INTERACTION WITH SHE3, MUTAGENESIS OF ASN-36; ARG-43; ARG-44; ARG-49; ARG-52 AND ARG-63.
[15]"ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex."
Gonsalvez G.B., Little J.L., Long R.M.
J. Biol. Chem. 279:46286-46294(2004) [PubMed: 15328357] [Abstract]
Cited for: FUNCTION.
[16]"Identification of a conserved RNA motif essential for She2p recognition and mRNA localization to the yeast bud."
Olivier C., Poirier G., Gendron P., Boisgontier A., Major F., Chartrand P.
Mol. Cell. Biol. 25:4752-4766(2005) [PubMed: 15899876] [Abstract]
Cited for: FUNCTION, RNA-BINDING.
[17]"Coordination of endoplasmic reticulum and mRNA localization to the yeast bud."
Schmid M., Jaedicke A., Du T.G., Jansen R.P.
Curr. Biol. 16:1538-1543(2006) [PubMed: 16890529] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[18]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, MASS SPECTROMETRY.
[19]"Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA."
Du T.G., Jellbauer S., Muller M., Schmid M., Niessing D., Jansen R.P.
EMBO Rep. 9:781-787(2008) [PubMed: 18566598] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[20]"Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p."
Shen Z., Paquin N., Forget A., Chartrand P.
Mol. Biol. Cell 20:2265-2275(2009) [PubMed: 19244342] [Abstract]
Cited for: FUNCTION, INTERACTION WITH LOC1; PUF6 AND SRP1, NUCLEUS LOCALIZATION SIGNAL, SUBCELLULAR LOCATION.
[21]"Formation of She2p tetramers is required for mRNA binding, mRNP assembly, and localization."
Muller M., Richter K., Heuck A., Kremmer E., Buchner J., Jansen R.P., Niessing D.
RNA 15:2002-2012(2009) [PubMed: 19710186] [Abstract]
Cited for: SUBUNIT, MUTAGENESIS OF LEU-130.
[22]"Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud."
Shen Z., St-Denis A., Chartrand P.
Genes Dev. 24:1914-1926(2010) [PubMed: 20713510] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RPO21; SPT4 AND SPT5.
[23]"She2p is a novel RNA binding protein with a basic helical hairpin motif."
Niessing D., Huttelmaier S., Zenklusen D., Singer R.H., Burley S.K.
Cell 119:491-502(2004) [PubMed: 15537539] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 6-239, SUBUNIT, FUNCTION, RNA-BINDING, MUTAGENESIS OF CYS-68 AND SER-120.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28130 Genomic DNA. Translation: CAA81971.1.
AY557902 Genomic DNA. Translation: AAS56228.1.
BK006944 Genomic DNA. Translation: DAA09031.1.
PIRS37959.
RefSeqNP_012792.1. NM_001179696.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XLYX-ray1.95A/B6-239[»]
ProteinModelPortalP36068.
SMRP36068. Positions 6-237.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1206N.
IntActP36068. 18 interactions.
MINTMINT-400410.
STRINGP36068.

Proteomic databases

PeptideAtlasP36068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL130C; YKL130C; YKL130C.
GeneID853728.
KEGGsce:YKL130C.
NMPDRfig|4932.3.peg.3773.

Organism-specific databases

CYGDYKL130c.
SGDS000001613. SHE2.

Phylogenomic databases

eggNOGfuNOG10958.
HOGENOMHBG202414.
OMAETDNIFL.
OrthoDBEOG4FR426.

Gene expression databases

ArrayExpressP36068.
GenevestigatorP36068.
GermOnlineYKL130C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR024261. RNA-bd_She2p.
[Graphical view]
Gene3DG3DSA:1.20.200.20. G3DSA:1.20.200.20. 1 hit.
PfamPF11435. She2p. 1 hit.
[Graphical view]
SUPFAMSSF116942. SSF116942. 1 hit.
ProtoNetSearch...

Other

NextBio974761.

Entry information

Entry nameSHE2_YEAST
AccessionPrimary (citable) accession number: P36068
Secondary accession number(s): D6VX65
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families