P36068 (SHE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SWI5-dependent HO expression protein 2

Gene names

Name:	SHE2
Ordered Locus Names:	YKL130C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, encoding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruites also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20 Ref.22 Ref.23
Subunit structure	Homodimer and Homotetramer. Interacts with LOC1, MYO4, PUF6, SHE3 and with RNA pol II subunits RPO21, SPT4 and SPT5. Ref.7 Ref.8 Ref.9 Ref.14 Ref.20 Ref.21 Ref.22 Ref.23
Subcellular location	Cytoplasm. Nucleus. Nucleus › nucleolus. Note: Shuttles between the nucleus and cytoplasm and is exported in an mRNA-dependent manner. Nucleolar transit is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins. The presence the nucleus is essential for PUF6 and LOC1 to bind the ASH1 mRNA. Ref.10 Ref.14 Ref.17 Ref.19 Ref.20
Miscellaneous	Present with 4070 molecules/cell in log phase SD medium. Ref.12
Sequence similarities	Belongs to the SHE2 family.

Ontologies

Keywords
Biological process	Transport mRNA transport
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular mRNA localization Inferred from direct assay Ref.7. Source: SGD mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW mating type switching Inferred from mutant phenotype. Source: SGD
Cellular component	cellular bud tip Inferred from direct assay Ref.7. Source: SGD cytoplasm Inferred from direct assay. Source: SGD nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mRNA binding Inferred from direct assay Ref.8. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYO4	P32492	4	EBI-26866,EBI-11681
SHE3	P38272	3	EBI-26866,EBI-21600

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

SWI5-dependent HO expression protein 2

PRO_0000203151

Regions

Motif

15 – 23

9

Nuclear localization signal

Amino acid modifications

Modified residue

166

1

Phosphoserine Ref.18

Experimental info

Mutagenesis

36

1

N → S: Prevents association with ASH1 and IST2 mRNAs and leads to their mislocalization. Ref.14

Mutagenesis

43

1

R → A: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14

Mutagenesis

44

1

R → A: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14

Mutagenesis

49

1

R → K: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14

Mutagenesis

52

1

R → A or K: Prevents association with ASH1 and mRNA and leads to its mislocalization. Ref.14

Mutagenesis

63

1

R → A or K: Prevents association with ASH1 and IST2 mRNAs and leads to their mislocalization. Ref.14

Mutagenesis

68

1

C → Y: Prevents dimerization and RNA-binding. Ref.23

Mutagenesis

120

1

S → Y: Prevents dimerization and RNA-binding. Ref.23

Mutagenesis

130

1

L → Y: Prevents tetramerization. Ref.21

Secondary structure

1

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246

Helix Strand Turn

Details...

Helix

13 – 42

30

Helix

46 – 48

3

Helix

49 – 71

23

Helix

74 – 77

4

Turn

86 – 88

3

Helix

92 – 118

27

Helix

120 – 129

10

Helix

132 – 134

3

Helix

138 – 161

24

Helix

167 – 169

3

Helix

172 – 179

8

Helix

205 – 235

31

Sequences

	Sequence		Length	Mass (Da)
	P36068 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5514B374655EFDBB	FASTA	246	28,251
	10 20 30 40 50 60 MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK 70 80 90 100 110 120 KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS 130 140 150 160 170 180 LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC 190 200 210 220 230 240 ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK FGKLKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Localization of ASH1 mRNA particles in living yeast." Bertrand E., Chartrand P., Schaefer M., Shenoy S.M., Singer R.H., Long R.M. Mol. Cell 2:437-445(1998) [PubMed: 9809065] [Abstract] Cited for: FUNCTION.
[5]	"Localization and anchoring of mRNA in budding yeast." Beach D.L., Salmon E.D., Bloom K. Curr. Biol. 9:569-578(1999) [PubMed: 10359695] [Abstract] Cited for: FUNCTION.
[6]	"Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins." Munchow S., Sauter C., Jansen R.P. J. Cell Sci. 112:1511-1518(1999) [PubMed: 10212145] [Abstract] Cited for: FUNCTION.
[7]	"She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p." Bohl F., Kruse C., Frank A., Ferring D., Jansen R.P. EMBO J. 19:5514-5524(2000) [PubMed: 11032818] [Abstract] Cited for: FUNCTION, RNA-BINDING, INTERACTION WITH SHE3 AND MYO4.
[8]	"She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA." Long R.M., Gu W., Lorimer E., Singer R.H., Chartrand P. EMBO J. 19:6592-6601(2000) [PubMed: 11101531] [Abstract] Cited for: FUNCTION, RNA-BINDING, INTERACTION WITH SHE3.
[9]	"The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p." Takizawa P.A., Vale R.D. Proc. Natl. Acad. Sci. U.S.A. 97:5273-5278(2000) [PubMed: 10792032] [Abstract] Cited for: RNA-BINDING, INTERACTION WITH MYO4.
[10]	"Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p." Kruse C., Jaedicke A., Beaudouin J., Bohl F., Ferring D., Guttler T., Ellenberg J., Jansen R.P. J. Cell Biol. 159:971-982(2002) [PubMed: 12499354] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]	"Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae." Estrada P., Kim J., Coleman J., Walker L., Dunn B., Takizawa P., Novick P., Ferro-Novick S. J. Cell Biol. 163:1255-1266(2003) [PubMed: 14691136] [Abstract] Cited for: FUNCTION.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis." Shepard K.A., Gerber A.P., Jambhekar A., Takizawa P.A., Brown P.O., Herschlag D., DeRisi J.L., Vale R.D. Proc. Natl. Acad. Sci. U.S.A. 100:11429-11434(2003) [PubMed: 13679573] [Abstract] Cited for: FUNCTION.
[14]	"RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex." Gonsalvez G.B., Lehmann K.A., Ho D.K., Stanitsa E.S., Williamson J.R., Long R.M. RNA 9:1383-1399(2003) [PubMed: 14561888] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, INTERACTION WITH SHE3, MUTAGENESIS OF ASN-36; ARG-43; ARG-44; ARG-49; ARG-52 AND ARG-63.
[15]	"ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex." Gonsalvez G.B., Little J.L., Long R.M. J. Biol. Chem. 279:46286-46294(2004) [PubMed: 15328357] [Abstract] Cited for: FUNCTION.
[16]	"Identification of a conserved RNA motif essential for She2p recognition and mRNA localization to the yeast bud." Olivier C., Poirier G., Gendron P., Boisgontier A., Major F., Chartrand P. Mol. Cell. Biol. 25:4752-4766(2005) [PubMed: 15899876] [Abstract] Cited for: FUNCTION, RNA-BINDING.
[17]	"Coordination of endoplasmic reticulum and mRNA localization to the yeast bud." Schmid M., Jaedicke A., Du T.G., Jansen R.P. Curr. Biol. 16:1538-1543(2006) [PubMed: 16890529] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[18]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, MASS SPECTROMETRY.
[19]	"Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA." Du T.G., Jellbauer S., Muller M., Schmid M., Niessing D., Jansen R.P. EMBO Rep. 9:781-787(2008) [PubMed: 18566598] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[20]	"Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p." Shen Z., Paquin N., Forget A., Chartrand P. Mol. Biol. Cell 20:2265-2275(2009) [PubMed: 19244342] [Abstract] Cited for: FUNCTION, INTERACTION WITH LOC1; PUF6 AND SRP1, NUCLEUS LOCALIZATION SIGNAL, SUBCELLULAR LOCATION.
[21]	"Formation of She2p tetramers is required for mRNA binding, mRNP assembly, and localization." Muller M., Richter K., Heuck A., Kremmer E., Buchner J., Jansen R.P., Niessing D. RNA 15:2002-2012(2009) [PubMed: 19710186] [Abstract] Cited for: SUBUNIT, MUTAGENESIS OF LEU-130.
[22]	"Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud." Shen Z., St-Denis A., Chartrand P. Genes Dev. 24:1914-1926(2010) [PubMed: 20713510] [Abstract] Cited for: FUNCTION, INTERACTION WITH RPO21; SPT4 AND SPT5.
[23]	"She2p is a novel RNA binding protein with a basic helical hairpin motif." Niessing D., Huttelmaier S., Zenklusen D., Singer R.H., Burley S.K. Cell 119:491-502(2004) [PubMed: 15537539] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 6-239, SUBUNIT, FUNCTION, RNA-BINDING, MUTAGENESIS OF CYS-68 AND SER-120.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z28130 Genomic DNA. Translation: CAA81971.1.
AY557902 Genomic DNA. Translation: AAS56228.1.
BK006944 Genomic DNA. Translation: DAA09031.1.

PIR

S37959.

RefSeq

NP_012792.1. NM_001179696.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XLY	X-ray	1.95	A/B	6-239	[»]

ProteinModelPortal

P36068.

SMR

P36068. Positions 6-237.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1206N.

IntAct

P36068. 18 interactions.

MINT

MINT-400410.

STRING

P36068.

Proteomic databases

PeptideAtlas

P36068.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL130C; YKL130C; YKL130C.

GeneID

853728.

KEGG

sce:YKL130C.

NMPDR

fig|4932.3.peg.3773.

Organism-specific databases

CYGD

YKL130c.

SGD

S000001613. SHE2.

Phylogenomic databases

eggNOG

fuNOG10958.

HOGENOM

HBG202414.

OMA

ETDNIFL.

OrthoDB

EOG4FR426.

Gene expression databases

ArrayExpress

P36068.

Genevestigator

P36068.

GermOnline

YKL130C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR024261. RNA-bd_She2p.
[Graphical view]

Gene3D

G3DSA:1.20.200.20. G3DSA:1.20.200.20. 1 hit.

Pfam

PF11435. She2p. 1 hit.
[Graphical view]

SUPFAM

SSF116942. SSF116942. 1 hit.

ProtoNet

Search...

Other

NextBio

974761.

Entry information

Entry name

SHE2_YEAST

Accession

Primary (citable) accession number: P36068
Secondary accession number(s): D6VX65

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program