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P36062

- AVT3_YEAST

UniProt

P36062 - AVT3_YEAST

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Protein

Vacuolar amino acid transporter 3

Gene
AVT3, YKL146W, YKL600
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine.1 Publication

GO - Molecular functioni

  1. L-glutamine transmembrane transporter activity Source: SGD
  2. L-isoleucine transmembrane transporter activity Source: SGD
  3. L-tyrosine transmembrane transporter activity Source: SGD
  4. transporter activity Source: SGD

GO - Biological processi

  1. amino acid transmembrane export from vacuole Source: SGD
  2. branched-chain amino acid transport Source: GOC
  3. cation transport Source: GOC
  4. glutamine transport Source: GOC
  5. L-alpha-amino acid transmembrane transport Source: GOC
  6. L-amino acid transport Source: GOC
  7. neutral amino acid transport Source: GOC
  8. tyrosine transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31921-MONOMER.

Protein family/group databases

TCDBi2.A.18.7.1. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 3
Gene namesi
Name:AVT3
Ordered Locus Names:YKL146W
ORF Names:YKL600
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL146w.
SGDiS000001629. AVT3.

Subcellular locationi

Vacuole membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei302 – 32221Helical; Reviewed predictionAdd
BLAST
Transmembranei329 – 34921Helical; Reviewed predictionAdd
BLAST
Transmembranei374 – 39421Helical; Reviewed predictionAdd
BLAST
Transmembranei412 – 43221Helical; Reviewed predictionAdd
BLAST
Transmembranei443 – 46321Helical; Reviewed predictionAdd
BLAST
Transmembranei483 – 50321Helical; Reviewed predictionAdd
BLAST
Transmembranei519 – 53921Helical; Reviewed predictionAdd
BLAST
Transmembranei561 – 58121Helical; Reviewed predictionAdd
BLAST
Transmembranei607 – 62721Helical; Reviewed predictionAdd
BLAST
Transmembranei630 – 65021Helical; Reviewed predictionAdd
BLAST
Transmembranei665 – 68521Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Vacuolar amino acid transporter 3PRO_0000093836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 591Phosphoserine1 Publication
Modified residuei119 – 1191Phosphoserine1 Publication
Modified residuei121 – 1211Phosphoserine2 Publications
Modified residuei165 – 1651Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36062.
PaxDbiP36062.

Expressioni

Gene expression databases

GenevestigatoriP36062.

Interactioni

Protein-protein interaction databases

BioGridi33990. 40 interactions.
DIPiDIP-4105N.
MINTiMINT-542142.
STRINGi4932.YKL146W.

Structurei

3D structure databases

ProteinModelPortaliP36062.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi261 – 27616Poly-GluAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00390000011245.
HOGENOMiHOG000185918.
KOiK14209.
OMAiKYNPYIK.
OrthoDBiEOG7BZW24.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36062-1 [UniParc]FASTAAdd to Basket

« Hide

MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR    50
SRSRSRKSSG TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN 100
NPEPHVVDAV ARHLIRNPSN SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP 150
SQPALSTSIP SQAPSFSNRK RSMSFSAASI ASSSHLNNNS EANGNPLAAI 200
GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF TRNFIEFLTL 250
YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV 300
KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT 350
TKDKVGVDGY GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ 400
VFSENFFHLK PGSISLATYI FAQVLIFVPL SLTRNIAKLS GTALIADLFI 450
LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK ADWSLFIGTA IFTFEGIGLL 500
IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG SDVKTVVLLN 550
FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK 600
VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL 650
HYKASILSGT SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ 692
Length:692
Mass (Da):75,459
Last modified:June 1, 1994 - v1
Checksum:i379054D69094A0F5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26877 Genomic DNA. Translation: CAA81508.1.
Z28146 Genomic DNA. Translation: CAA81988.1.
BK006944 Genomic DNA. Translation: DAA09016.1.
PIRiS37976.
RefSeqiNP_012776.1. NM_001179712.1.

Genome annotation databases

EnsemblFungiiYKL146W; YKL146W; YKL146W.
GeneIDi853710.
KEGGisce:YKL146W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26877 Genomic DNA. Translation: CAA81508.1 .
Z28146 Genomic DNA. Translation: CAA81988.1 .
BK006944 Genomic DNA. Translation: DAA09016.1 .
PIRi S37976.
RefSeqi NP_012776.1. NM_001179712.1.

3D structure databases

ProteinModelPortali P36062.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33990. 40 interactions.
DIPi DIP-4105N.
MINTi MINT-542142.
STRINGi 4932.YKL146W.

Protein family/group databases

TCDBi 2.A.18.7.1. the amino acid/auxin permease (aaap) family.

Proteomic databases

MaxQBi P36062.
PaxDbi P36062.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL146W ; YKL146W ; YKL146W .
GeneIDi 853710.
KEGGi sce:YKL146W.

Organism-specific databases

CYGDi YKL146w.
SGDi S000001629. AVT3.

Phylogenomic databases

eggNOGi COG0814.
GeneTreei ENSGT00390000011245.
HOGENOMi HOG000185918.
KOi K14209.
OMAi KYNPYIK.
OrthoDBi EOG7BZW24.

Enzyme and pathway databases

BioCyci YEAST:G3O-31921-MONOMER.

Miscellaneous databases

NextBioi 974716.
PROi P36062.

Gene expression databases

Genevestigatori P36062.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
    Russnak R., Konczal D., McIntire S.L.
    J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121 AND SER-165, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-121, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAVT3_YEAST
AccessioniPrimary (citable) accession number: P36062
Secondary accession number(s): D6VX50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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