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Protein

Vacuolar amino acid transporter 3

Gene

AVT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine.1 Publication

GO - Molecular functioni

  1. L-glutamine transmembrane transporter activity Source: SGD
  2. L-isoleucine transmembrane transporter activity Source: SGD
  3. L-tyrosine transmembrane transporter activity Source: SGD

GO - Biological processi

  1. amino acid transmembrane export from vacuole Source: SGD
  2. branched-chain amino acid transport Source: GOC
  3. cation transmembrane transport Source: GOC
  4. cation transport Source: GOC
  5. glutamine transport Source: GOC
  6. L-alpha-amino acid transmembrane transport Source: GOC
  7. L-amino acid transport Source: GOC
  8. neutral amino acid transport Source: GOC
  9. tyrosine transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31921-MONOMER.
ReactomeiREACT_301247. Proton-coupled neutral amino acid transporters.
REACT_341203. Amino acid transport across the plasma membrane.

Protein family/group databases

TCDBi2.A.18.7.1. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 3
Gene namesi
Name:AVT3
Ordered Locus Names:YKL146W
ORF Names:YKL600
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKL146w.
SGDiS000001629. AVT3.

Subcellular locationi

Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei302 – 32221HelicalSequence AnalysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence AnalysisAdd
BLAST
Transmembranei374 – 39421HelicalSequence AnalysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence AnalysisAdd
BLAST
Transmembranei443 – 46321HelicalSequence AnalysisAdd
BLAST
Transmembranei483 – 50321HelicalSequence AnalysisAdd
BLAST
Transmembranei519 – 53921HelicalSequence AnalysisAdd
BLAST
Transmembranei561 – 58121HelicalSequence AnalysisAdd
BLAST
Transmembranei607 – 62721HelicalSequence AnalysisAdd
BLAST
Transmembranei630 – 65021HelicalSequence AnalysisAdd
BLAST
Transmembranei665 – 68521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Vacuolar amino acid transporter 3PRO_0000093836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 591Phosphoserine1 Publication
Modified residuei119 – 1191Phosphoserine1 Publication
Modified residuei121 – 1211Phosphoserine2 Publications
Modified residuei165 – 1651Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36062.
PaxDbiP36062.

Expressioni

Gene expression databases

GenevestigatoriP36062.

Interactioni

Protein-protein interaction databases

BioGridi33990. 43 interactions.
DIPiDIP-4105N.
MINTiMINT-542142.
STRINGi4932.YKL146W.

Structurei

3D structure databases

ProteinModelPortaliP36062.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi261 – 27616Poly-GluAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00390000011245.
HOGENOMiHOG000185918.
InParanoidiP36062.
KOiK14209.
OMAiKYNPYIK.
OrthoDBiEOG7BZW24.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR
60 70 80 90 100
SRSRSRKSSG TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN
110 120 130 140 150
NPEPHVVDAV ARHLIRNPSN SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP
160 170 180 190 200
SQPALSTSIP SQAPSFSNRK RSMSFSAASI ASSSHLNNNS EANGNPLAAI
210 220 230 240 250
GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF TRNFIEFLTL
260 270 280 290 300
YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV
310 320 330 340 350
KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT
360 370 380 390 400
TKDKVGVDGY GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ
410 420 430 440 450
VFSENFFHLK PGSISLATYI FAQVLIFVPL SLTRNIAKLS GTALIADLFI
460 470 480 490 500
LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK ADWSLFIGTA IFTFEGIGLL
510 520 530 540 550
IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG SDVKTVVLLN
560 570 580 590 600
FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK
610 620 630 640 650
VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL
660 670 680 690
HYKASILSGT SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ
Length:692
Mass (Da):75,459
Last modified:May 31, 1994 - v1
Checksum:i379054D69094A0F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81508.1.
Z28146 Genomic DNA. Translation: CAA81988.1.
BK006944 Genomic DNA. Translation: DAA09016.1.
PIRiS37976.
RefSeqiNP_012776.1. NM_001179712.1.

Genome annotation databases

EnsemblFungiiYKL146W; YKL146W; YKL146W.
GeneIDi853710.
KEGGisce:YKL146W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81508.1.
Z28146 Genomic DNA. Translation: CAA81988.1.
BK006944 Genomic DNA. Translation: DAA09016.1.
PIRiS37976.
RefSeqiNP_012776.1. NM_001179712.1.

3D structure databases

ProteinModelPortaliP36062.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33990. 43 interactions.
DIPiDIP-4105N.
MINTiMINT-542142.
STRINGi4932.YKL146W.

Protein family/group databases

TCDBi2.A.18.7.1. the amino acid/auxin permease (aaap) family.

Proteomic databases

MaxQBiP36062.
PaxDbiP36062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL146W; YKL146W; YKL146W.
GeneIDi853710.
KEGGisce:YKL146W.

Organism-specific databases

CYGDiYKL146w.
SGDiS000001629. AVT3.

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00390000011245.
HOGENOMiHOG000185918.
InParanoidiP36062.
KOiK14209.
OMAiKYNPYIK.
OrthoDBiEOG7BZW24.

Enzyme and pathway databases

BioCyciYEAST:G3O-31921-MONOMER.
ReactomeiREACT_301247. Proton-coupled neutral amino acid transporters.
REACT_341203. Amino acid transport across the plasma membrane.

Miscellaneous databases

NextBioi974716.
PROiP36062.

Gene expression databases

GenevestigatoriP36062.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
    Russnak R., Konczal D., McIntire S.L.
    J. Biol. Chem. 276:23849-23857(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121 AND SER-165, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-121, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAVT3_YEAST
AccessioniPrimary (citable) accession number: P36062
Secondary accession number(s): D6VX50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 1994
Last sequence update: May 31, 1994
Last modified: March 31, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.