P36062 (AVT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar amino acid transporter 3

Gene names

Name:	AVT3
Ordered Locus Names:	YKL146W
ORF Names:	YKL600

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Ref.4
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4.
Sequence similarities	Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amino acid transmembrane export from vacuole Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	fungal-type vacuole membrane Inferred from mutant phenotype Ref.4. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	L-glutamine transmembrane transporter activity Inferred from mutant phenotype Ref.4. Source: SGD L-isoleucine transmembrane transporter activity Inferred from mutant phenotype Ref.4. Source: SGD L-tyrosine transmembrane transporter activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 692	692	Vacuolar amino acid transporter 3		PRO_0000093836
Regions
	Transmembrane	302 – 322	21	Helical; Potential
	Transmembrane	329 – 349	21	Helical; Potential
	Transmembrane	374 – 394	21	Helical; Potential
	Transmembrane	412 – 432	21	Helical; Potential
	Transmembrane	443 – 463	21	Helical; Potential
	Transmembrane	483 – 503	21	Helical; Potential
	Transmembrane	519 – 539	21	Helical; Potential
	Transmembrane	561 – 581	21	Helical; Potential
	Transmembrane	607 – 627	21	Helical; Potential
	Transmembrane	630 – 650	21	Helical; Potential
	Transmembrane	665 – 685	21	Helical; Potential
	Compositional bias	261 – 276	16	Poly-Glu
Amino acid modifications
	Modified residue	58	1	Phosphoserine Ref.6
	Modified residue	59	1	Phosphoserine Ref.5 Ref.6 Ref.7
	Modified residue	61	1	Phosphothreonine Ref.6
	Modified residue	119	1	Phosphoserine Ref.7
	Modified residue	121	1	Phosphoserine Ref.7
	Modified residue	143	1	Phosphoserine Ref.7
	Modified residue	145	1	Phosphoserine Ref.7
	Modified residue	157	1	Phosphothreonine Ref.7
	Modified residue	158	1	Phosphoserine Ref.7
	Modified residue	165	1	Phosphoserine Ref.7
	Modified residue	283	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P36062 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 379054D69094A0F5	FASTA	692	75,459
	10 20 30 40 50 60 MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR SRSRSRKSSG 70 80 90 100 110 120 TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN NPEPHVVDAV ARHLIRNPSN 130 140 150 160 170 180 SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP SQPALSTSIP SQAPSFSNRK RSMSFSAASI 190 200 210 220 230 240 ASSSHLNNNS EANGNPLAAI GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF 250 260 270 280 290 300 TRNFIEFLTL YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV 310 320 330 340 350 360 KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT TKDKVGVDGY 370 380 390 400 410 420 GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ VFSENFFHLK PGSISLATYI 430 440 450 460 470 480 FAQVLIFVPL SLTRNIAKLS GTALIADLFI LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK 490 500 510 520 530 540 ADWSLFIGTA IFTFEGIGLL IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG 550 560 570 580 590 600 SDVKTVVLLN FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK 610 620 630 640 650 660 VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL HYKASILSGT 670 680 690 SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A family of yeast proteins mediating bidirectional vacuolar amino acid transport." Russnak R., Konczal D., McIntire S.L. J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-59 AND THR-61, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59; SER-119; SER-121; SER-143; SER-145; THR-157; SER-158; SER-165 AND THR-283, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26877 Genomic DNA. Translation: CAA81508.1. Z28146 Genomic DNA. Translation: CAA81988.1. BK006944 Genomic DNA. Translation: DAA09016.1.
PIR	S37976.
RefSeq	NP_012776.1. NM_001179712.1.
3D structure databases
ProteinModelPortal	P36062.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4105N.
MINT	MINT-542142.
STRING	4932.YKL146W.
Protein family/group databases
TCDB	2.A.18.7.1. amino acid/auxin permease (AAAP) family.
Proteomic databases
PaxDb	P36062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL146W; YKL146W; YKL146W.
GeneID	853710.
KEGG	sce:YKL146W.
Organism-specific databases
CYGD	YKL146w.
SGD	S000001629. AVT3.
Phylogenomic databases
eggNOG	COG0814.
GeneTree	ENSGT00390000011245.
HOGENOM	HOG000185918.
KO	K14209.
OMA	PGGFRRN.
OrthoDB	EOG4R2731.
Gene expression databases
Genevestigator	P36062.
GermOnline	YKL146W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013057. AA_transpt_TM. [Graphical view]
Pfam	PF01490. Aa_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974716.

Entry information

Entry name

AVT3_YEAST

Accession

Primary (citable) accession number: P36062
Secondary accession number(s): D6VX50

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program