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P36062

- AVT3_YEAST

UniProt

P36062 - AVT3_YEAST

Protein

Vacuolar amino acid transporter 3

Gene

AVT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine.1 Publication

    GO - Molecular functioni

    1. L-glutamine transmembrane transporter activity Source: SGD
    2. L-isoleucine transmembrane transporter activity Source: SGD
    3. L-tyrosine transmembrane transporter activity Source: SGD
    4. transporter activity Source: SGD

    GO - Biological processi

    1. amino acid transmembrane export from vacuole Source: SGD
    2. branched-chain amino acid transport Source: GOC
    3. cation transport Source: GOC
    4. glutamine transport Source: GOC
    5. L-alpha-amino acid transmembrane transport Source: GOC
    6. L-amino acid transport Source: GOC
    7. neutral amino acid transport Source: GOC
    8. tyrosine transport Source: GOC

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31921-MONOMER.

    Protein family/group databases

    TCDBi2.A.18.7.1. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar amino acid transporter 3
    Gene namesi
    Name:AVT3
    Ordered Locus Names:YKL146W
    ORF Names:YKL600
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL146w.
    SGDiS000001629. AVT3.

    Subcellular locationi

    Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. fungal-type vacuole membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 692692Vacuolar amino acid transporter 3PRO_0000093836Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei59 – 591Phosphoserine1 Publication
    Modified residuei119 – 1191Phosphoserine1 Publication
    Modified residuei121 – 1211Phosphoserine2 Publications
    Modified residuei165 – 1651Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36062.
    PaxDbiP36062.

    Expressioni

    Gene expression databases

    GenevestigatoriP36062.

    Interactioni

    Protein-protein interaction databases

    BioGridi33990. 40 interactions.
    DIPiDIP-4105N.
    MINTiMINT-542142.
    STRINGi4932.YKL146W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36062.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei302 – 32221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei329 – 34921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei374 – 39421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei443 – 46321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei483 – 50321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei519 – 53921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei561 – 58121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei607 – 62721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei630 – 65021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei665 – 68521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi261 – 27616Poly-GluAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0814.
    GeneTreeiENSGT00390000011245.
    HOGENOMiHOG000185918.
    KOiK14209.
    OMAiKYNPYIK.
    OrthoDBiEOG7BZW24.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36062-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR    50
    SRSRSRKSSG TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN 100
    NPEPHVVDAV ARHLIRNPSN SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP 150
    SQPALSTSIP SQAPSFSNRK RSMSFSAASI ASSSHLNNNS EANGNPLAAI 200
    GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF TRNFIEFLTL 250
    YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV 300
    KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT 350
    TKDKVGVDGY GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ 400
    VFSENFFHLK PGSISLATYI FAQVLIFVPL SLTRNIAKLS GTALIADLFI 450
    LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK ADWSLFIGTA IFTFEGIGLL 500
    IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG SDVKTVVLLN 550
    FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK 600
    VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL 650
    HYKASILSGT SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ 692
    Length:692
    Mass (Da):75,459
    Last modified:June 1, 1994 - v1
    Checksum:i379054D69094A0F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26877 Genomic DNA. Translation: CAA81508.1.
    Z28146 Genomic DNA. Translation: CAA81988.1.
    BK006944 Genomic DNA. Translation: DAA09016.1.
    PIRiS37976.
    RefSeqiNP_012776.1. NM_001179712.1.

    Genome annotation databases

    EnsemblFungiiYKL146W; YKL146W; YKL146W.
    GeneIDi853710.
    KEGGisce:YKL146W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26877 Genomic DNA. Translation: CAA81508.1 .
    Z28146 Genomic DNA. Translation: CAA81988.1 .
    BK006944 Genomic DNA. Translation: DAA09016.1 .
    PIRi S37976.
    RefSeqi NP_012776.1. NM_001179712.1.

    3D structure databases

    ProteinModelPortali P36062.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33990. 40 interactions.
    DIPi DIP-4105N.
    MINTi MINT-542142.
    STRINGi 4932.YKL146W.

    Protein family/group databases

    TCDBi 2.A.18.7.1. the amino acid/auxin permease (aaap) family.

    Proteomic databases

    MaxQBi P36062.
    PaxDbi P36062.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL146W ; YKL146W ; YKL146W .
    GeneIDi 853710.
    KEGGi sce:YKL146W.

    Organism-specific databases

    CYGDi YKL146w.
    SGDi S000001629. AVT3.

    Phylogenomic databases

    eggNOGi COG0814.
    GeneTreei ENSGT00390000011245.
    HOGENOMi HOG000185918.
    KOi K14209.
    OMAi KYNPYIK.
    OrthoDBi EOG7BZW24.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31921-MONOMER.

    Miscellaneous databases

    NextBioi 974716.
    PROi P36062.

    Gene expression databases

    Genevestigatori P36062.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
      Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
      Russnak R., Konczal D., McIntire S.L.
      J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121 AND SER-165, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-121, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAVT3_YEAST
    AccessioniPrimary (citable) accession number: P36062
    Secondary accession number(s): D6VX50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3