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Reviewed, UniProtKB/Swiss-Prot P36062 (AVT3_YEAST)

Last modified November 24, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Vacuolar amino acid transporter 3
Gene names
Name: AVT3
Ordered Locus Names: YKL146W
ORF Names: YKL600
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Ref.3

Subcellular location

Vacuole membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 692692Vacuolar amino acid transporter 3
PRO_0000093836

Regions

Transmembrane302 – 32221 Potential
Transmembrane329 – 34921 Potential
Transmembrane374 – 39421 Potential
Transmembrane412 – 43221 Potential
Transmembrane443 – 46321 Potential
Transmembrane483 – 50321 Potential
Transmembrane519 – 53921 Potential
Transmembrane561 – 58121 Potential
Transmembrane607 – 62721 Potential
Transmembrane630 – 65021 Potential
Transmembrane665 – 68521 Potential
Compositional bias261 – 27616Poly-Glu

Amino acid modifications

Modified residue581Phosphoserine Ref.5
Modified residue591Phosphoserine Ref.5 Ref.4 Ref.6
Modified residue611Phosphothreonine Ref.5
Modified residue1191Phosphoserine Ref.6
Modified residue1211Phosphoserine Ref.6
Modified residue1431Phosphoserine Ref.6
Modified residue1451Phosphoserine Ref.6
Modified residue1571Phosphothreonine Ref.6
Modified residue1581Phosphoserine Ref.6
Modified residue1651Phosphoserine Ref.6
Modified residue2831Phosphothreonine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P36062-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 379054D69094A0F5

FASTA69275,459
        10         20         30         40         50         60 
MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR SRSRSRKSSG 

        70         80         90        100        110        120 
TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN NPEPHVVDAV ARHLIRNPSN 

       130        140        150        160        170        180 
SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP SQPALSTSIP SQAPSFSNRK RSMSFSAASI 

       190        200        210        220        230        240 
ASSSHLNNNS EANGNPLAAI GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF 

       250        260        270        280        290        300 
TRNFIEFLTL YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV 

       310        320        330        340        350        360 
KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT TKDKVGVDGY 

       370        380        390        400        410        420 
GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ VFSENFFHLK PGSISLATYI 

       430        440        450        460        470        480 
FAQVLIFVPL SLTRNIAKLS GTALIADLFI LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK 

       490        500        510        520        530        540 
ADWSLFIGTA IFTFEGIGLL IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG 

       550        560        570        580        590        600 
SDVKTVVLLN FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK 

       610        620        630        640        650        660 
VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL HYKASILSGT 

       670        680        690 
SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
Russnak R., Konczal D., McIntire S.L.
J. Biol. Chem. 276:23849-23857(2001) [PubMed: 11274162] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, MASS SPECTROMETRY.
[5]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-59 AND THR-61, MASS SPECTROMETRY.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59; SER-119; SER-121; SER-143; SER-145; THR-157; SER-158; SER-165 AND THR-283, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z26877 Genomic DNA. Translation: CAA81508.1.
Z28146 Genomic DNA. Translation: CAA81988.1.
PIRS37976.
RefSeqNP_012776.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4105N.
IntActP36062. 2 interactions.
STRINGP36062.

Protein family/group databases

TCDB2.A.18.7.1. amino acid/auxin permease (AAAP) family.

Proteomic databases

PRIDEP36062.

Genome annotation databases

EnsemblYKL146W; YKL146W; YKL146W; Saccharomyces cerevisiae. [Genome view]
GeneID853710.
KEGGsce:YKL146W.
NMPDRfig|4932.3.peg.3755.

Organism-specific databases

CYGDYKL146w.
SGDS000001629. AVT3.

Phylogenomic databases

HOGENOMP36062.
OMAPGGFRRN
OrthoDBEOG91NW33

Gene expression databases

ArrayExpressP36062.
GenevestigatorP36062.
GermOnlineYKL146W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974716.

Entry information

Entry nameAVT3_YEAST
AccessionPrimary (citable) accession number: P36062
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 24, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents