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Protein

Signal recognition particle receptor subunit beta

Gene

SRP102

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. Has GTPase activity. May mediate the membrane association of SRPR.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901GTP; via amide nitrogen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 539GTP1 Publication
Nucleotide bindingi66 – 694GTP1 Publication
Nucleotide bindingi154 – 1574GTP1 Publication

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: SGD
  • signal recognition particle binding Source: SGD

GO - Biological processi

  • protein targeting to ER Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31924-MONOMER.

Protein family/group databases

TCDBi3.A.5.8.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle receptor subunit beta
Short name:
SR-beta
Gene namesi
Name:SRP102
Ordered Locus Names:YKL154W
ORF Names:YKL609
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL154W.
SGDiS000001637. SRP102.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2317HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: SGD
  • signal recognition particle receptor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244Signal recognition particle receptor subunit betaPRO_0000101230Add
BLAST

Proteomic databases

MaxQBiP36057.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SRP101P329165EBI-18091,EBI-18098

Protein-protein interaction databases

BioGridi33983. 49 interactions.
DIPiDIP-4753N.
IntActiP36057. 15 interactions.
MINTiMINT-365016.

Structurei

Secondary structure

1
244
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 445Combined sources
Helixi51 – 6010Combined sources
Beta strandi73 – 753Combined sources
Helixi78 – 803Combined sources
Beta strandi84 – 874Combined sources
Helixi92 – 954Combined sources
Helixi96 – 10510Combined sources
Helixi106 – 1083Combined sources
Beta strandi109 – 1179Combined sources
Helixi126 – 14217Combined sources
Beta strandi149 – 1546Combined sources
Helixi164 – 18724Combined sources
Helixi214 – 2163Combined sources
Beta strandi217 – 2193Combined sources
Beta strandi221 – 2255Combined sources
Turni228 – 2314Combined sources
Helixi234 – 24310Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NRJX-ray1.70B31-244[»]
2GEDX-ray2.20A/B36-183[»]
ProteinModelPortaliP36057.
SMRiP36057. Positions 36-244.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36057.

Family & Domainsi

Sequence similaritiesi

Belongs to the SRP receptor beta subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000048347.
HOGENOMiHOG000248720.
InParanoidiP36057.
KOiK12272.
OMAiWENWIDE.
OrthoDBiEOG7K3TXW.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR019009. SRP_receptor_beta_su.
[Graphical view]
PfamiPF09439. SRPRB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P36057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSNTLIIAC LLVIGTTIAL IAVQKASSKT GIKQKSYQPS IIIAGPQNSG
60 70 80 90 100
KTSLLTLLTT DSVRPTVVSQ EPLSAADYDG SGVTLVDFPG HVKLRYKLSD
110 120 130 140 150
YLKTRAKFVK GLIFMVDSTV DPKKLTTTAE FLVDILSITE SSCENGIDIL
160 170 180 190 200
IACNKSELFT ARPPSKIKDA LESEIQKVIE RRKKSLNEVE RKINEEDYAE
210 220 230 240
NTLDVLQSTD GFKFANLEAS VVAFEGSINK RKISQWREWI DEKL
Length:244
Mass (Da):26,974
Last modified:June 1, 1994 - v1
Checksum:iEA4AE9126B2A04B5
GO

Sequence cautioni

The sequence CAA81499.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81499.1. Different initiation.
Z28154 Genomic DNA. Translation: CAA81995.1.
BK006944 Genomic DNA. Translation: DAA09010.1.
PIRiS37984.
RefSeqiNP_012768.1. NM_001179720.1.

Genome annotation databases

EnsemblFungiiYKL154W; YKL154W; YKL154W.
GeneIDi853702.
KEGGisce:YKL154W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81499.1. Different initiation.
Z28154 Genomic DNA. Translation: CAA81995.1.
BK006944 Genomic DNA. Translation: DAA09010.1.
PIRiS37984.
RefSeqiNP_012768.1. NM_001179720.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NRJX-ray1.70B31-244[»]
2GEDX-ray2.20A/B36-183[»]
ProteinModelPortaliP36057.
SMRiP36057. Positions 36-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33983. 49 interactions.
DIPiDIP-4753N.
IntActiP36057. 15 interactions.
MINTiMINT-365016.

Protein family/group databases

TCDBi3.A.5.8.1. the general secretory pathway (sec) family.

Proteomic databases

MaxQBiP36057.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL154W; YKL154W; YKL154W.
GeneIDi853702.
KEGGisce:YKL154W.

Organism-specific databases

EuPathDBiFungiDB:YKL154W.
SGDiS000001637. SRP102.

Phylogenomic databases

GeneTreeiENSGT00510000048347.
HOGENOMiHOG000248720.
InParanoidiP36057.
KOiK12272.
OMAiWENWIDE.
OrthoDBiEOG7K3TXW.

Enzyme and pathway databases

BioCyciYEAST:G3O-31924-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP36057.
NextBioi974696.
PROiP36057.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR019009. SRP_receptor_beta_su.
[Graphical view]
PfamiPF09439. SRPRB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor."
    Schwartz T., Blobel G.
    Cell 112:793-803(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-244 IN COMPLEX WITH GTP AND SRP101, SUBUNIT.

Entry informationi

Entry nameiSRPB_YEAST
AccessioniPrimary (citable) accession number: P36057
Secondary accession number(s): D6VX44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.