Signal recognition particle receptor subunit beta - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36057 (SRPB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Signal recognition particle receptor subunit beta
Short name=SR-beta

Gene names

Name:	SRP102
Ordered Locus Names:	YKL154W
ORF Names:	YKL609

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. Has GTPase activity. May mediate the membrane association of SRPR.
Subunit structure	Heterodimer of an alpha and a beta chain. Ref.5
Subcellular location	Endoplasmic reticulum membrane Potential.
Miscellaneous	Present with 8250 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the SRP receptor beta subunit family.
Sequence caution	The sequence CAA81499.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	GTP-binding Nucleotide-binding
Molecular function	Receptor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: SGD
Cellular component	integral to endoplasmic reticulum membrane Inferred from direct assay. Source: SGD signal recognition particle receptor complex Traceable author statement. Source: SGD
Molecular function	GTP binding Inferred from physical interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct receptor activity Inferred from electronic annotation. Source: UniProtKB-KW signal recognition particle binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRP101	P32916	5	EBI-18091,EBI-18098

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 244

244

Signal recognition particle receptor subunit beta

PRO_0000101230

Regions

Transmembrane

7 – 23

Helical; Potential

Nucleotide binding

45 – 53

GTP

Nucleotide binding

66 – 69

GTP

Nucleotide binding

154 – 157

GTP

Sites

Binding site

GTP; via amide nitrogen

Secondary structure

244

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P36057 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: EA4AE9126B2A04B5
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FASTA

244

26,974

        10         20         30         40         50         60 
MLSNTLIIAC LLVIGTTIAL IAVQKASSKT GIKQKSYQPS IIIAGPQNSG KTSLLTLLTT 

        70         80         90        100        110        120 
DSVRPTVVSQ EPLSAADYDG SGVTLVDFPG HVKLRYKLSD YLKTRAKFVK GLIFMVDSTV 

       130        140        150        160        170        180 
DPKKLTTTAE FLVDILSITE SSCENGIDIL IACNKSELFT ARPPSKIKDA LESEIQKVIE 

       190        200        210        220        230        240 
RRKKSLNEVE RKINEEDYAE NTLDVLQSTD GFKFANLEAS VVAFEGSINK RKISQWREWI 


DEKL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor." Schwartz T., Blobel G. Cell 112:793-803(2003) [PubMed: 12654246] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-244 IN COMPLEX WITH GTP AND SRP101, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z26877 Genomic DNA. Translation: CAA81499.1. Different initiation.
Z28154 Genomic DNA. Translation: CAA81995.1.
BK006944 Genomic DNA. Translation: DAA09010.1.

PIR

S37984.

RefSeq

NP_012768.1. NM_001179720.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NRJ	X-ray	1.70	B	31-244	[»]
2GED	X-ray	2.20	A/B	36-244	[»]

ProteinModelPortal

P36057.

SMR

P36057. Positions 36-244.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4753N.

IntAct

P36057. 5 interactions.

MINT

MINT-365016.

STRING

P36057.

Protein family/group databases

TCDB

3.A.5.8.1. general secretory pathway (Sec) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL154W; YKL154W; YKL154W.

GeneID

853702.

KEGG

sce:YKL154W.

NMPDR

fig|4932.3.peg.3747.

Organism-specific databases

CYGD

YKL154w.

SGD

S000001637. SRP102.

Phylogenomic databases

eggNOG

fuNOG10734.

GeneTree

EFGT00050000004466.

HOGENOM

HBG398153.

OMA

IDILIAC.

OrthoDB

EOG42RHHQ.

Gene expression databases

ArrayExpress

P36057.

Genevestigator

P36057.

GermOnline

YKL154W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR019009. SRP_receptor_beta_su.
[Graphical view]

K12272.

Pfam

PF09439. SRPRB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

974696.

Entry information

Entry name

SRPB_YEAST

Accession

Primary (citable) accession number: P36057
Secondary accession number(s): D6VX44

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents