P36053 (ELF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor 1

Gene names

Name:	ELF1
Ordered Locus Names:	YKL160W
ORF Names:	YKL614

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions. Ref.7
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 5860 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ELOF1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription by chromatin organization Inferred from mutant phenotype Ref.7. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	transcription elongation factor complex Inferred from direct assay PubMed 20818391. Source: SGD
Molecular_function	RNA polymerase II core binding Inferred from direct assay PubMed 20818391. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Transcription elongation factor 1		PRO_0000120950
Sites
	Metal binding	25	1	Zinc By similarity
	Metal binding	28	1	Zinc By similarity
	Metal binding	49	1	Zinc By similarity
	Metal binding	52	1	Zinc By similarity
Amino acid modifications
	Modified residue	55	1	Phosphoserine Ref.11
	Modified residue	117	1	Phosphoserine Ref.9 Ref.10 Ref.11
	Modified residue	124	1	Phosphoserine Ref.9 Ref.10 Ref.11
	Modified residue	142	1	Phosphoserine Ref.8 Ref.9 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P36053 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 0741A4F14D6CD705	FASTA	145	16,157
	10 20 30 40 50 60 MGKRKKSTRK PTKRLVQKLD TKFNCLFCNH EKSVSCTLDK KNSIGTLSCK ICGQSFQTRI 70 80 90 100 110 120 NSLSQPVDVY SDWFDAVEEV NSGRGSDTDD GDEGSDSDYE SDSEQDAKTQ NDGEIDSDEE 130 140 EVDSDEERIG QVKRGRGALV DSDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Identification and characterization of Elf1, a conserved transcription elongation factor in Saccharomyces cerevisiae." Prather D., Krogan N.J., Emili A., Greenblatt J.F., Winston F. Mol. Cell. Biol. 25:10122-10135(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117; SER-124 AND SER-142, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117 AND SER-124, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-117; SER-124 AND SER-142, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26877 Genomic DNA. Translation: CAA81494.1. Z28160 Genomic DNA. Translation: CAA82002.1. AY558355 Genomic DNA. Translation: AAS56681.1. BK006944 Genomic DNA. Translation: DAA09005.1.
PIR	S37791.
RefSeq	NP_012762.1. NM_001179726.1.
3D structure databases
ProteinModelPortal	P36053.
SMR	P36053. Positions 2-78.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2119N.
IntAct	P36053. 2 interactions.
MINT	MINT-508271.
STRING	4932.YKL160W.
Proteomic databases
PaxDb	P36053.
PeptideAtlas	P36053.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL160W; YKL160W; YKL160W.
GeneID	853697.
KEGG	sce:YKL160W.
Organism-specific databases
CYGD	YKL160w.
SGD	S000001643. ELF1.
Phylogenomic databases
eggNOG	COG4888.
GeneTree	ENSGT00390000000053.
HOGENOM	HOG000159293.
OMA	YSDWFDA.
OrthoDB	EOG4K6KF0.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31928-MONOMER.
Gene expression databases
Genevestigator	P36053.
GermOnline	YKL160W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007808. Elf1. [Graphical view]
PANTHER	PTHR20934. PTHR20934. 1 hit.
Pfam	PF05129. Elf1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974681.

Entry information

Entry name

ELF1_YEAST

Accession

Primary (citable) accession number: P36053
Secondary accession number(s): D6VX39

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program