Reviewed, UniProtKB/Swiss-Prot P36053 (ELF1_YEAST)

Names and origin

Protein names

Recommended name:
    Transcription elongation factor 1

Gene names

Name:	ELF1
Ordered Locus Names:	YKL160W
ORF Names:	YKL614

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions. Ref.6
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 5860 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the ELOF1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA elongation from RNA polymerase II promoter Ref.6 Inferred from genetic interaction. Source: SGD chromatin organization Ref.6 Inferred from genetic interaction. Source: SGD negative regulation of transposition, RNA-mediated Inferred from mutant phenotype. Source: SGD regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	RNA polymerase II transcription elongation factor activity Ref.6 Inferred from genetic interaction. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Transcription elongation factor 1		PRO_0000120950
Sites
	Metal binding	25	1	Zinc By similarity
	Metal binding	28	1	Zinc By similarity
	Metal binding	49	1	Zinc By similarity
	Metal binding	52	1	Zinc By similarity
Amino acid modifications
	Modified residue	55	1	Phosphoserine Ref.10
	Modified residue	117	1	Phosphoserine Ref.10 Ref.8 Ref.9
	Modified residue	124	1	Phosphoserine Ref.10 Ref.8 Ref.9
	Modified residue	142	1	Phosphoserine Ref.10 Ref.8 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P36053-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 0741A4F14D6CD705	FASTA	145	16,157
	10 20 30 40 50 60 MGKRKKSTRK PTKRLVQKLD TKFNCLFCNH EKSVSCTLDK KNSIGTLSCK ICGQSFQTRI 70 80 90 100 110 120 NSLSQPVDVY SDWFDAVEEV NSGRGSDTDD GDEGSDSDYE SDSEQDAKTQ NDGEIDSDEE 130 140 EVDSDEERIG QVKRGRGALV DSDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Identification and characterization of Elf1, a conserved transcription elongation factor in Saccharomyces cerevisiae." Prather D., Krogan N.J., Emili A., Greenblatt J.F., Winston F. Mol. Cell. Biol. 25:10122-10135(2005) [PubMed: 16260625] [Abstract] Cited for: FUNCTION.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117; SER-124 AND SER-142, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117 AND SER-124, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-117; SER-124 AND SER-142, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z26877 Genomic DNA. Translation: CAA81494.1. Z28160 Genomic DNA. Translation: CAA82002.1. AY558355 Genomic DNA. Translation: AAS56681.1.
PIR	S37791.
RefSeq	NP_012762.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2119N.
IntAct	P36053. 2 interactions.
STRING	P36053.
Proteomic databases
PeptideAtlas	P36053.
Genome annotation databases
Ensembl	YKL160W; YKL160W; YKL160W; Saccharomyces cerevisiae. [Genome view]
GeneID	853697.
KEGG	sce:YKL160W.
NMPDR	fig|4932.3.peg.3741.
Organism-specific databases
CYGD	YKL160w.
SGD	S000001643. ELF1.
Phylogenomic databases
HOGENOM	HBG630513.
OMA	YSDWFDA.
OrthoDB	EOG9PRV72.
Gene expression databases
ArrayExpress	P36053.
Genevestigator	P36053.
GermOnline	YKL160W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007808. DUF701_Zn_bd. [Graphical view]
PANTHER	PTHR20934. DUF701_Zn_bd. 1 hit.
Pfam	PF05129. Elf1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974681.

Entry information

Entry name

ELF1_YEAST

Accession

Primary (citable) accession number: P36053

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 15, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)