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Protein

GPI ethanolamine phosphate transferase 1

Gene

MCD4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor. Ethanolamine phosphate on the alpha-1,4-linked mannose is essential for further mannosylation by GPI10 and is necessary for an efficient recognition of GPI lipids and GPI proteins by the GPI transamidase, for the efficient transport of GPI anchored proteins from endoplasmic reticulum to Golgi and for the physiological incorporation of ceramides into GPI anchors by lipid remodeling. Also involved in non-mitochondrial ATP movements across membrane and participates in Golgi and endoplasmic reticulum function, Also required for the incorporation of BGL2 into the cell wall.7 Publications

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • mannose-ethanolamine phosphotransferase activity Source: SGD
  • sulfuric ester hydrolase activity Source: InterPro

GO - Biological processi

  • ATP transport Source: SGD
  • cell wall organization Source: UniProtKB-KW
  • GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation, GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31933-MONOMER.
ReactomeiR-SCE-162710. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Protein family/group databases

TCDBi9.A.6.1.1. the atp exporter (atp-e) family.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI ethanolamine phosphate transferase 1 (EC:2.-.-.-)
Alternative name(s):
Morphogenesis checkpoint-dependent protein 4
Supersecretion of u-PA protein 21
Gene namesi
Name:MCD4
Synonyms:FSR2, SSU21, ZRG16
Ordered Locus Names:YKL165C
ORF Names:YKL619
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL165C.
SGDiS000001648. MCD4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Topological domaini31 – 457427LumenalSequence analysisAdd
BLAST
Transmembranei458 – 47821HelicalSequence analysisAdd
BLAST
Topological domaini479 – 48810CytoplasmicSequence analysis
Transmembranei489 – 50921HelicalSequence analysisAdd
BLAST
Topological domaini510 – 5123LumenalSequence analysis
Transmembranei513 – 53321HelicalSequence analysisAdd
BLAST
Topological domaini534 – 55320CytoplasmicSequence analysisAdd
BLAST
Transmembranei554 – 57421HelicalSequence analysisAdd
BLAST
Topological domaini575 – 5762LumenalSequence analysis
Transmembranei577 – 59721HelicalSequence analysisAdd
BLAST
Topological domaini598 – 5981CytoplasmicSequence analysis
Transmembranei599 – 61921HelicalSequence analysisAdd
BLAST
Topological domaini620 – 6267LumenalSequence analysis
Transmembranei627 – 64721HelicalSequence analysisAdd
BLAST
Topological domaini648 – 6558CytoplasmicSequence analysis
Transmembranei656 – 67621HelicalSequence analysisAdd
BLAST
Topological domaini677 – 68711LumenalSequence analysisAdd
BLAST
Transmembranei688 – 70821HelicalSequence analysisAdd
BLAST
Topological domaini709 – 72012CytoplasmicSequence analysisAdd
BLAST
Transmembranei721 – 74121HelicalSequence analysisAdd
BLAST
Topological domaini742 – 77635LumenalSequence analysisAdd
BLAST
Transmembranei777 – 79721HelicalSequence analysisAdd
BLAST
Topological domaini798 – 80710CytoplasmicSequence analysis
Transmembranei808 – 82821HelicalSequence analysisAdd
BLAST
Topological domaini829 – 84820LumenalSequence analysisAdd
BLAST
Transmembranei849 – 86921HelicalSequence analysisAdd
BLAST
Topological domaini870 – 88516CytoplasmicSequence analysisAdd
BLAST
Transmembranei886 – 90621HelicalSequence analysisAdd
BLAST
Topological domaini907 – 91913LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: GO_Central
  • fungal-type cell wall Source: SGD
  • fungal-type vacuole Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi227 – 2271G → E in mcd4-174; defective in endoplasmic reticulum-to-Golgi transport of GPI-anchored proteins. 1 Publication
Mutagenesisi302 – 3021P → L: Induces auxotrophy for ethanolamine. 1 Publication
Mutagenesisi916 – 9172KK → SS: No effect on subcellular location. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919GPI ethanolamine phosphate transferase 1PRO_0000211412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence analysis
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP36051.

Interactioni

Protein-protein interaction databases

BioGridi33972. 27 interactions.
DIPiDIP-8272N.
IntActiP36051. 1 interaction.
MINTiMINT-4493130.

Structurei

3D structure databases

ProteinModelPortaliP36051.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017600.
HOGENOMiHOG000170818.
InParanoidiP36051.
KOiK05285.
OMAiGWISYAS.
OrthoDBiEOG7SXWBS.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR007070. GPI_EtnP_transferase_1.
IPR017852. GPI_EtnP_transferase_1_C.
IPR000917. Sulfatase_N.
[Graphical view]
PANTHERiPTHR12250. PTHR12250. 1 hit.
PfamiPF04987. PigN. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P36051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWNKTRTTLL AVGVLFHLFY LWSIFDIYFI SPLVHGMSPY QSTPTPPAKR
60 70 80 90 100
LFLIVGDGLR ADTTFDKVTH PVSGKTEFLA PFIRSLVMNN ATYGISHTRM
110 120 130 140 150
PTESRPGHVA MIAGFYEDVS AVTKGWKSNP VNFDSFFNQS THTYSFGSPD
160 170 180 190 200
ILPMFKDGAS DPNKVDTWMY DHTFEDFTQS SIELDAFVFR HLDQLFHNST
210 220 230 240 250
LNSTLDYEIR QDGNVFFLHL LGCDTAGHSY RPYSAEYYDN VKYIDDQIPI
260 270 280 290 300
LIDKVNKFFA DDKTAFIFTA DHGMSAFGSH GDGHPNNTRT PLVAWGAGLN
310 320 330 340 350
KPVHNPFPVS DNYTENWELS SIKRNDVKQA DIASLMSYLI GVNYPKNSVG
360 370 380 390 400
ELPIAYIDGK ESDKLAALYN NARSILEQYL VKQDEVIDSQ FFYKEYFKFV
410 420 430 440 450
EKSHSHYLEE IETLIQRISE GENYLEQEAI TLTEELMQIT LEGLHYLTTY
460 470 480 490 500
NWRFIRTIVT FGFVGWIFFS FIIFLKSFIL ENVIDDQKAS PLSHAVFGSI
510 520 530 540 550
GILLNWILFY QHSPFNFYMY LLFPLYFWSY IFTNRSVLRS GIKEFFKGTS
560 570 580 590 600
PWKRVLITIS IISVYEGIVY GFFHRWTFTL ITNILAFYPF ICGVRELSVN
610 620 630 640 650
ILWIITSVLL STFTLFDAVK IEDLNQIHLA GLLIILSAFY ALYKIHSRIN
660 670 680 690 700
SYTRAIFAIQ ISLVAAMLAV THRSVISLQL RQGLPRESQV AGWIIFFVSL
710 720 730 740 750
FVMPILHYRK PNNDYKVRLL IIYLTFAPSF IILTISFESL FYFLFTSYMV
760 770 780 790 800
QWIEIENKIK EMKTQKDENW LQVLRVSVIG FFLLQVAFFG TGNVASISSF
810 820 830 840 850
SLESVCRLLP IFDPFLMGAL LMLKLIIPYG LLSTCLGILN LKLNFKDYTI
860 870 880 890 900
SSLIISMSDI LSLNFFYLLR TEGSWLDIGI TISNYCLAIL SSLFMLILEV
910
LGHVLLKNVI IQDKTKKTQ
Length:919
Mass (Da):105,693
Last modified:June 1, 1994 - v1
Checksum:iB8F1D0BF80792C6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81489.1.
Z28165 Genomic DNA. Translation: CAA82007.1.
BK006944 Genomic DNA. Translation: DAA09000.1.
PIRiS37786.
RefSeqiNP_012756.1. NM_001179731.1.

Genome annotation databases

EnsemblFungiiYKL165C; YKL165C; YKL165C.
GeneIDi853690.
KEGGisce:YKL165C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81489.1.
Z28165 Genomic DNA. Translation: CAA82007.1.
BK006944 Genomic DNA. Translation: DAA09000.1.
PIRiS37786.
RefSeqiNP_012756.1. NM_001179731.1.

3D structure databases

ProteinModelPortaliP36051.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33972. 27 interactions.
DIPiDIP-8272N.
IntActiP36051. 1 interaction.
MINTiMINT-4493130.

Protein family/group databases

TCDBi9.A.6.1.1. the atp exporter (atp-e) family.

Proteomic databases

MaxQBiP36051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL165C; YKL165C; YKL165C.
GeneIDi853690.
KEGGisce:YKL165C.

Organism-specific databases

EuPathDBiFungiDB:YKL165C.
SGDiS000001648. MCD4.

Phylogenomic databases

GeneTreeiENSGT00390000017600.
HOGENOMiHOG000170818.
InParanoidiP36051.
KOiK05285.
OMAiGWISYAS.
OrthoDBiEOG7SXWBS.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciYEAST:G3O-31933-MONOMER.
ReactomeiR-SCE-162710. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

PROiP36051.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR007070. GPI_EtnP_transferase_1.
IPR017852. GPI_EtnP_transferase_1_C.
IPR000917. Sulfatase_N.
[Graphical view]
PANTHERiPTHR12250. PTHR12250. 1 hit.
PfamiPF04987. PigN. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast."
    Gaynor E.C., Mondesert G., Grimme S.J., Reed S.I., Orlean P., Emr S.D.
    Mol. Biol. Cell 10:627-648(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, MUTAGENESIS OF GLY-227 AND 916-LYS-LYS-917.
  5. "A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast."
    Packeiser A.N., Urakov V.N., Polyakova Y.A., Shimanova N.I., Shcherbukhin V.D., Smirnov V.N., Ter-Avanesyan M.D.
    Yeast 15:1485-1501(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis."
    Storey M.K., Wu W.-I., Voelker D.R.
    Biochim. Biophys. Acta 1532:234-247(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF PRO-302.
  7. "Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall."
    Kalebina T.S., Laurinavichiute D.K., Packeiser A.N., Morenkov O.S., Ter-Avanesyan M.D., Kulaev I.S.
    FEMS Microbiol. Lett. 210:81-85(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase."
    Zhong X., Malhotra R., Guidotti G.
    J. Biol. Chem. 278:33436-33444(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae."
    Maneesri J., Azuma M., Sakai Y., Igarashi K., Matsumoto T., Fukuda H., Kondo A., Ooshima H.
    J. Biosci. Bioeng. 99:354-360(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi."
    Zhu Y., Vionnet C., Conzelmann A.
    J. Biol. Chem. 281:19830-19839(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMCD4_YEAST
AccessioniPrimary (citable) accession number: P36051
Secondary accession number(s): D6VX34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Target of the inhibitor of GPI biosynthesis YW3548/BE49385A.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.