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Reviewed, UniProtKB/Swiss-Prot P36051 (MCD4_YEAST)

Last modified November 3, 2009. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    GPI ethanolamine phosphate transferase 1
    EC=2.-.-.-
Alternative name(s):
    Morphogenesis checkpoint-dependent protein 4
    Supersecretion of u-PA protein 21
Gene names
Name: MCD4
Synonyms: FSR2, SSU21, ZRG16
Ordered Locus Names: YKL165C
ORF Names: YKL619
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor. Ethanolamine phosphate on the alpha-1,4-linked mannose is essential for further mannosylation by GPI10 and is necessary for an efficient recognition of GPI lipids and GPI proteins by the GPI transamidase, for the efficient transport of GPI anchored proteins from endoplasmic reticulum to Golgi and for the physiological incorporation of ceramides into GPI anchors by lipid remodeling. Also involved in non-mitochondrial ATP movements across membrane and participates in Golgi and endoplasmic reticulum function, Also required for the incorporation of BGL2 into the cell wall. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.9 Ref.10

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Ref.3 Ref.4 Ref.8

Post-translational modification

N-glycosylated. Ref.3

Miscellaneous

Target of the inhibitor of GPI biosynthesis YW3548/BE49385A.

Sequence similarities

Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 919919GPI ethanolamine phosphate transferase 1
PRO_0000211412

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3021 Potential
Topological domain31 – 457427Lumenal Potential
Transmembrane458 – 47821 Potential
Topological domain479 – 48810Cytoplasmic Potential
Transmembrane489 – 50921 Potential
Topological domain510 – 5123Lumenal Potential
Transmembrane513 – 53321 Potential
Topological domain534 – 55320Cytoplasmic Potential
Transmembrane554 – 57421 Potential
Topological domain575 – 5762Lumenal Potential
Transmembrane577 – 59721 Potential
Topological domain5981Cytoplasmic Potential
Transmembrane599 – 61921 Potential
Topological domain620 – 6267Lumenal Potential
Transmembrane627 – 64721 Potential
Topological domain648 – 6558Cytoplasmic Potential
Transmembrane656 – 67621 Potential
Topological domain677 – 68711Lumenal Potential
Transmembrane688 – 70821 Potential
Topological domain709 – 72012Cytoplasmic Potential
Transmembrane721 – 74121 Potential
Topological domain742 – 77635Lumenal Potential
Transmembrane777 – 79721 Potential
Topological domain798 – 80710Cytoplasmic Potential
Transmembrane808 – 82821 Potential
Topological domain829 – 84820Lumenal Potential
Transmembrane849 – 86921 Potential
Topological domain870 – 88516Cytoplasmic Potential
Transmembrane886 – 90621 Potential
Topological domain907 – 91913Lumenal Potential

Amino acid modifications

Modified residue1041Phosphoserine Ref.11
Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation1981N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation3121N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2271G → E in mcd4-174; defective in endoplasmic reticulum-to-Golgi transport of GPI-anchored proteins. Ref.3
Mutagenesis3021P → L: Induces auxotrophy for ethanolamine. Ref.5
Mutagenesis916 – 9172KK → SS: No effect on subcellular location.

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Sequences

Sequence LengthMass (Da)Tools
P36051-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B8F1D0BF80792C6A

FASTA919105,693
        10         20         30         40         50         60 
MWNKTRTTLL AVGVLFHLFY LWSIFDIYFI SPLVHGMSPY QSTPTPPAKR LFLIVGDGLR 

        70         80         90        100        110        120 
ADTTFDKVTH PVSGKTEFLA PFIRSLVMNN ATYGISHTRM PTESRPGHVA MIAGFYEDVS 

       130        140        150        160        170        180 
AVTKGWKSNP VNFDSFFNQS THTYSFGSPD ILPMFKDGAS DPNKVDTWMY DHTFEDFTQS 

       190        200        210        220        230        240 
SIELDAFVFR HLDQLFHNST LNSTLDYEIR QDGNVFFLHL LGCDTAGHSY RPYSAEYYDN 

       250        260        270        280        290        300 
VKYIDDQIPI LIDKVNKFFA DDKTAFIFTA DHGMSAFGSH GDGHPNNTRT PLVAWGAGLN 

       310        320        330        340        350        360 
KPVHNPFPVS DNYTENWELS SIKRNDVKQA DIASLMSYLI GVNYPKNSVG ELPIAYIDGK 

       370        380        390        400        410        420 
ESDKLAALYN NARSILEQYL VKQDEVIDSQ FFYKEYFKFV EKSHSHYLEE IETLIQRISE 

       430        440        450        460        470        480 
GENYLEQEAI TLTEELMQIT LEGLHYLTTY NWRFIRTIVT FGFVGWIFFS FIIFLKSFIL 

       490        500        510        520        530        540 
ENVIDDQKAS PLSHAVFGSI GILLNWILFY QHSPFNFYMY LLFPLYFWSY IFTNRSVLRS 

       550        560        570        580        590        600 
GIKEFFKGTS PWKRVLITIS IISVYEGIVY GFFHRWTFTL ITNILAFYPF ICGVRELSVN 

       610        620        630        640        650        660 
ILWIITSVLL STFTLFDAVK IEDLNQIHLA GLLIILSAFY ALYKIHSRIN SYTRAIFAIQ 

       670        680        690        700        710        720 
ISLVAAMLAV THRSVISLQL RQGLPRESQV AGWIIFFVSL FVMPILHYRK PNNDYKVRLL 

       730        740        750        760        770        780 
IIYLTFAPSF IILTISFESL FYFLFTSYMV QWIEIENKIK EMKTQKDENW LQVLRVSVIG 

       790        800        810        820        830        840 
FFLLQVAFFG TGNVASISSF SLESVCRLLP IFDPFLMGAL LMLKLIIPYG LLSTCLGILN 

       850        860        870        880        890        900 
LKLNFKDYTI SSLIISMSDI LSLNFFYLLR TEGSWLDIGI TISNYCLAIL SSLFMLILEV 

       910 
LGHVLLKNVI IQDKTKKTQ

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References

« Hide 'large scale' references
[1]"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast."
Gaynor E.C., Mondesert G., Grimme S.J., Reed S.I., Orlean P., Emr S.D.
Mol. Biol. Cell 10:627-648(1999) [PubMed: 10069808] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, MUTAGENESIS OF GLY-227 AND 916-LYS-LYS-917.
[4]"A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast."
Packeiser A.N., Urakov V.N., Polyakova Y.A., Shimanova N.I., Shcherbukhin V.D., Smirnov V.N., Ter-Avanesyan M.D.
Yeast 15:1485-1501(1999) [PubMed: 10514566] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis."
Storey M.K., Wu W.-I., Voelker D.R.
Biochim. Biophys. Acta 1532:234-247(2001) [PubMed: 11470244] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF PRO-302.
[6]"Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall."
Kalebina T.S., Laurinavichiute D.K., Packeiser A.N., Morenkov O.S., Ter-Avanesyan M.D., Kulaev I.S.
FEMS Microbiol. Lett. 210:81-85(2002) [PubMed: 12023081] [Abstract]
Cited for: FUNCTION.
[7]"ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase."
Zhong X., Malhotra R., Guidotti G.
J. Biol. Chem. 278:33436-33444(2003) [PubMed: 12807869] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae."
Maneesri J., Azuma M., Sakai Y., Igarashi K., Matsumoto T., Fukuda H., Kondo A., Ooshima H.
J. Biosci. Bioeng. 99:354-360(2005) [PubMed: 16233801] [Abstract]
Cited for: FUNCTION.
[10]"Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi."
Zhu Y., Vionnet C., Conzelmann A.
J. Biol. Chem. 281:19830-19839(2006) [PubMed: 16704983] [Abstract]
Cited for: FUNCTION.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Z26877 Genomic DNA. Translation: CAA81489.1.
Z28165 Genomic DNA. Translation: CAA82007.1.
PIRS37786.
RefSeqNP_012756.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:8272N.
IntActP36051. 13 interactions.
STRINGP36051.

Protein family/group databases

TCDB9.A.6.1.1. ATP exporter (ATP-E) family.

Genome annotation databases

EnsemblYKL165C; YKL165C; YKL165C; Saccharomyces cerevisiae. [Genome view]
GeneID853690.
GenomeReviewsGene locus YKL165C in contig Y13137_GR.
KEGGsce:YKL165C.
NMPDRfig|4932.3.peg.3735.

Organism-specific databases

CYGDYKL165c.
SGDS000001648. MCD4.

Phylogenomic databases

HOGENOMP36051.
OMAKDYGSWL.

Gene expression databases

ArrayExpressP36051.
GenevestigatorP36051.
GermOnlineYKL165C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR007070. GPI_EtnP_transferase_1.
IPR017852. GPI_EtnP_transferase_1_C.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PANTHERPTHR12250. PigN. 1 hit.
PfamPF04987. PigN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974664.

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Entry information

Entry nameMCD4_YEAST
AccessionPrimary (citable) accession number: P36051
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 3, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents