Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

rRNA-processing protein EBP2

Gene

EBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • nuclear division Source: SGD
  • ribosomal large subunit biogenesis Source: GO_Central
  • rRNA processing Source: SGD

Keywordsi

Biological processRibosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31939-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA-processing protein EBP2
Alternative name(s):
EBNA1-binding protein homolog
Gene namesi
Name:EBP2
Ordered Locus Names:YKL172W
ORF Names:YKL636
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL172W
SGDiS000001655 EBP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36 – 37KK → RR: Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-61 and R-62. 1 Publication2
Mutagenesisi61 – 62KK → RR: Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-36 and R-37. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001200001 – 427rRNA-processing protein EBP2Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104PhosphoserineCombined sources1
Modified residuei177PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP36049
PaxDbiP36049
PRIDEiP36049

PTM databases

iPTMnetiP36049

Interactioni

Subunit structurei

Interacts with LOC1, NOP12, SIZ2, ULS1 and WSS1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi33966, 201 interactors
DIPiDIP-6507N
IntActiP36049, 60 interactors
MINTiP36049
STRINGi4932.YKL172W

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi199 – 207Combined sources9
Beta strandi213 – 215Combined sources3
Helixi217 – 220Combined sources4
Turni236 – 239Combined sources4
Helixi241 – 260Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6ELZelectron microscopy3.30J1-427[»]
6EM1electron microscopy3.60J1-427[»]
6EM3electron microscopy3.20J1-427[»]
6EM4electron microscopy4.10J1-427[»]
6EM5electron microscopy4.30J1-427[»]
ProteinModelPortaliP36049
SMRiP36049
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili45 – 174Sequence analysisAdd BLAST130
Coiled coili234 – 265Sequence analysisAdd BLAST32
Coiled coili291 – 348Sequence analysisAdd BLAST58

Sequence similaritiesi

Belongs to the EBP2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014984
HOGENOMiHOG000176390
InParanoidiP36049
KOiK14823
OMAiAFYKVCQ
OrthoDBiEOG092C5MPO

Family and domain databases

InterProiView protein in InterPro
IPR008610 Ebp2
PANTHERiPTHR13028 PTHR13028, 3 hits
PfamiView protein in Pfam
PF05890 Ebp2, 1 hit

Sequencei

Sequence statusi: Complete.

P36049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK
60 70 80 90 100
LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELKK MQEQDATEAQ
110 120 130 140 150
KHMSGDEDES GDDREEEEEE EEEEEGRLDL EKLAKSDSES EDDSESENDS
160 170 180 190 200
EEDEDVVAKE ESEEKEEQEE EQDVPLSDVE FDSDADVVPH HKLTVNNTKA
210 220 230 240 250
MKHALERVQL PWKKHSFQEH QSVTSETNTD EHIKDIYDDT ERELAFYKQS
260 270 280 290 300
LDAVLVARDE LKRLKVPFKR PLDYFAEMVK SDEHMDKIKG KLIEEASDKK
310 320 330 340 350
AREEARRQRQ LKKFGKQVQN ATLQKRQLEK RETLEKIKSL KNKRKHNEID
360 370 380 390 400
HSEFNVGVEE EVEGKRFDRG RPNGKRAAKN AKYGQGGMKR FKRKNDATSS
410 420
ADVSGFSSRK MKGKTNRPGK SRRARRF
Length:427
Mass (Da):49,734
Last modified:June 1, 1994 - v1
Checksum:i4A11F6CDF779DB5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA Translation: CAA81515.1
Z28172 Genomic DNA Translation: CAA82014.1
BK006944 Genomic DNA Translation: DAA08994.1
PIRiS38002
RefSeqiNP_012749.1, NM_001179738.1

Genome annotation databases

EnsemblFungiiYKL172W; YKL172W; YKL172W
GeneIDi853682
KEGGisce:YKL172W

Similar proteinsi

Entry informationi

Entry nameiEBP2_YEAST
AccessioniPrimary (citable) accession number: P36049
Secondary accession number(s): D6VX28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health