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Protein

rRNA-processing protein EBP2

Gene

EBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • nuclear division Source: SGD
  • ribosomal large subunit biogenesis Source: GO_Central
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31939-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA-processing protein EBP2
Alternative name(s):
EBNA1-binding protein homolog
Gene namesi
Name:EBP2
Ordered Locus Names:YKL172W
ORF Names:YKL636
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL172W.
SGDiS000001655. EBP2.

Subcellular locationi

GO - Cellular componenti

  • nuclear periphery Source: SGD
  • nucleolus Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 372KK → RR: Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-61 and R-62. 1 Publication
Mutagenesisi61 – 622KK → RR: Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-36 and R-37. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427rRNA-processing protein EBP2PRO_0000120000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041PhosphoserineCombined sources
Modified residuei177 – 1771PhosphoserineCombined sources
Modified residuei183 – 1831PhosphoserineCombined sources

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP36049.

PTM databases

iPTMnetiP36049.

Interactioni

Subunit structurei

Interacts with LOC1, NOP12, SIZ2, ULS1 and WSS1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-6289,EBI-6289
BRX1Q082355EBI-6289,EBI-3775
CGR1P531883EBI-6289,EBI-23731
DBP10Q123893EBI-6289,EBI-5644
LOC1P435866EBI-6289,EBI-22906
MPS3P470694EBI-6289,EBI-25811
NOC2P397443EBI-6289,EBI-29259
NOP4P378385EBI-6289,EBI-12122
NSA2P400783EBI-6289,EBI-22681
PRP43P531313EBI-6289,EBI-505
RRP12Q127543EBI-6289,EBI-30678
SSF1P387894EBI-6289,EBI-18160

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi33966. 115 interactions.
DIPiDIP-6507N.
IntActiP36049. 48 interactions.
MINTiMINT-671580.

Structurei

3D structure databases

ProteinModelPortaliP36049.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili45 – 174130Sequence analysisAdd
BLAST
Coiled coili234 – 26532Sequence analysisAdd
BLAST
Coiled coili291 – 34858Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the EBP2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014984.
HOGENOMiHOG000176390.
InParanoidiP36049.
KOiK14823.
OMAiGMKRFKR.
OrthoDBiEOG092C5MPO.

Family and domain databases

InterProiIPR008610. Ebp2.
[Graphical view]
PANTHERiPTHR13028. PTHR13028. 1 hit.
PfamiPF05890. Ebp2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK
60 70 80 90 100
LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELKK MQEQDATEAQ
110 120 130 140 150
KHMSGDEDES GDDREEEEEE EEEEEGRLDL EKLAKSDSES EDDSESENDS
160 170 180 190 200
EEDEDVVAKE ESEEKEEQEE EQDVPLSDVE FDSDADVVPH HKLTVNNTKA
210 220 230 240 250
MKHALERVQL PWKKHSFQEH QSVTSETNTD EHIKDIYDDT ERELAFYKQS
260 270 280 290 300
LDAVLVARDE LKRLKVPFKR PLDYFAEMVK SDEHMDKIKG KLIEEASDKK
310 320 330 340 350
AREEARRQRQ LKKFGKQVQN ATLQKRQLEK RETLEKIKSL KNKRKHNEID
360 370 380 390 400
HSEFNVGVEE EVEGKRFDRG RPNGKRAAKN AKYGQGGMKR FKRKNDATSS
410 420
ADVSGFSSRK MKGKTNRPGK SRRARRF
Length:427
Mass (Da):49,734
Last modified:June 1, 1994 - v1
Checksum:i4A11F6CDF779DB5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81515.1.
Z28172 Genomic DNA. Translation: CAA82014.1.
BK006944 Genomic DNA. Translation: DAA08994.1.
PIRiS38002.
RefSeqiNP_012749.1. NM_001179738.1.

Genome annotation databases

EnsemblFungiiYKL172W; YKL172W; YKL172W.
GeneIDi853682.
KEGGisce:YKL172W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81515.1.
Z28172 Genomic DNA. Translation: CAA82014.1.
BK006944 Genomic DNA. Translation: DAA08994.1.
PIRiS38002.
RefSeqiNP_012749.1. NM_001179738.1.

3D structure databases

ProteinModelPortaliP36049.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33966. 115 interactions.
DIPiDIP-6507N.
IntActiP36049. 48 interactions.
MINTiMINT-671580.

PTM databases

iPTMnetiP36049.

Proteomic databases

MaxQBiP36049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL172W; YKL172W; YKL172W.
GeneIDi853682.
KEGGisce:YKL172W.

Organism-specific databases

EuPathDBiFungiDB:YKL172W.
SGDiS000001655. EBP2.

Phylogenomic databases

GeneTreeiENSGT00390000014984.
HOGENOMiHOG000176390.
InParanoidiP36049.
KOiK14823.
OMAiGMKRFKR.
OrthoDBiEOG092C5MPO.

Enzyme and pathway databases

BioCyciYEAST:G3O-31939-MONOMER.

Miscellaneous databases

PROiP36049.

Family and domain databases

InterProiIPR008610. Ebp2.
[Graphical view]
PANTHERiPTHR13028. PTHR13028. 1 hit.
PfamiPF05890. Ebp2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEBP2_YEAST
AccessioniPrimary (citable) accession number: P36049
Secondary accession number(s): D6VX28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.