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P36048

- SN114_YEAST

UniProt

P36048 - SN114_YEAST

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Protein

Pre-mRNA-splicing factor SNU114

Gene
SNU114, GIN10, YKL173W, YKL637
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 1478GTP By similarity
Nucleotide bindingi214 – 2185GTP By similarity
Nucleotide bindingi268 – 2714GTP By similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: SGD
  3. U5 snRNA binding Source: SGD

GO - Biological processi

  1. generation of catalytic spliceosome for first transesterification step Source: SGD
  2. GTP catabolic process Source: GOC
  3. mRNA splicing, via spliceosome Source: SGD
  4. spliceosomal tri-snRNP complex assembly Source: SGD
  5. spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31940-MONOMER.
ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SNU114
Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component
Growth inhibitory protein 10
Gene namesi
Name:SNU114
Synonyms:GIN10
Ordered Locus Names:YKL173W
ORF Names:YKL637
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL173w.
SGDiS000001656. SNU114.

Subcellular locationi

GO - Cellular componenti

  1. U4/U6 x U5 tri-snRNP complex Source: SGD
  2. U5 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10081008Pre-mRNA-splicing factor SNU114PRO_0000091565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851Phosphoserine1 Publication
Modified residuei88 – 881Phosphothreonine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36048.
PaxDbiP36048.
PeptideAtlasiP36048.

Expressioni

Gene expression databases

GenevestigatoriP36048.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Interacts (via C-terminus) with CWC21. Interacts (via N-terminus) with PRP8 (via SCwid domain).4 Publications

Protein-protein interaction databases

BioGridi33965. 133 interactions.
DIPiDIP-759N.
IntActiP36048. 48 interactions.
MINTiMINT-426147.
STRINGi4932.YKL173W.

Structurei

3D structure databases

ProteinModelPortaliP36048.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 338208tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1478G1 By similarity
Regioni188 – 1925G2 By similarity
Regioni214 – 2174G3 By similarity
Regioni268 – 2714G4 By similarity
Regioni315 – 3173G5 By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0480.
HOGENOMiHOG000231589.
KOiK12853.
OMAiYGWDSLA.
OrthoDBiEOG7966SQ.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36048-1 [UniParc]FASTAAdd to Basket

« Hide

MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES     50
RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL 100
QEHTIFTQLK KNIPKTRYNR DYMLSMANIP ERIINVGVIG PLHSGKTSLM 150
DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN LKQEIDRGLS IKLNGSTLLC 200
TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID VVEGVTFVVE 250
QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK 300
GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG 350
SVYYHKGNFR TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL 400
RSNFRVNLSQ EALQYDPQPF LKHVLQLIFR QQTGLVDAIT RCYQPFELFD 450
NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG AEWSLVRIYS GLLKRGDTVR 500
ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE EIGLLGGRYV 550
YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE 600
AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL 650
YMDCLLYDLR ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE 700
ENLPGLSISV AAEPMDSKMI QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI 750
LRTEYGWDSL ASRNVWSFYN GNVLINDTLP DEISPELLSK YKEQIIQGFY 800
WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL 850
LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL 900
EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF 950
IPKLKPAPIN SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ 1000
LRENGLVP 1008
Length:1,008
Mass (Da):114,041
Last modified:June 1, 1994 - v1
Checksum:i5CE30CEFF154D013
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26878 Genomic DNA. Translation: CAA81514.1.
Z28173 Genomic DNA. Translation: CAA82015.1.
D28149 Genomic DNA. Translation: BAA05682.1.
BK006944 Genomic DNA. Translation: DAA08993.1.
PIRiS38003.
RefSeqiNP_012748.1. NM_001179739.1.

Genome annotation databases

EnsemblFungiiYKL173W; YKL173W; YKL173W.
GeneIDi853681.
KEGGisce:YKL173W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26878 Genomic DNA. Translation: CAA81514.1 .
Z28173 Genomic DNA. Translation: CAA82015.1 .
D28149 Genomic DNA. Translation: BAA05682.1 .
BK006944 Genomic DNA. Translation: DAA08993.1 .
PIRi S38003.
RefSeqi NP_012748.1. NM_001179739.1.

3D structure databases

ProteinModelPortali P36048.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33965. 133 interactions.
DIPi DIP-759N.
IntActi P36048. 48 interactions.
MINTi MINT-426147.
STRINGi 4932.YKL173W.

Proteomic databases

MaxQBi P36048.
PaxDbi P36048.
PeptideAtlasi P36048.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL173W ; YKL173W ; YKL173W .
GeneIDi 853681.
KEGGi sce:YKL173W.

Organism-specific databases

CYGDi YKL173w.
SGDi S000001656. SNU114.

Phylogenomic databases

eggNOGi COG0480.
HOGENOMi HOG000231589.
KOi K12853.
OMAi YGWDSLA.
OrthoDBi EOG7966SQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-31940-MONOMER.
Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi 974643.

Gene expression databases

Genevestigatori P36048.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SMARTi SM00838. EFG_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
    Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
    EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Akada R., Yamamoto J., Yamashita I.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 823-846.
    Strain: ATCC 26786 / X2180-1A.
  7. "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2."
    Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R.
    EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
    Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
    Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.
  12. "Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."
    Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D.
    RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CWC21 AND PRP8.
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSN114_YEAST
AccessioniPrimary (citable) accession number: P36048
Secondary accession number(s): D6VX27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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