P36048 (SN114_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor SNU114

Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component
Growth inhibitory protein 10

Gene names

Name:	SNU114
Synonyms:	GIN10
Ordered Locus Names:	YKL173W
ORF Names:	YKL637

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1008 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
Subunit structure	Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Interacts (via C-terminus) with CWC21. Interacts (via N-terminus) with PRP8 (via SCwid domain). Ref.4 Ref.5 Ref.12 Ref.13
Subcellular location	Nucleus.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	assembly of spliceosomal tri-snRNP Inferred from mutant phenotype PubMed 16540695. Source: SGD generation of catalytic spliceosome for first transesterification step Inferred from genetic interaction PubMed 15911574. Source: SGD spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Inferred from mutant phenotype PubMed 16540695. Source: SGD
Cellular_component	U4/U6 x U5 tri-snRNP complex Inferred from direct assay PubMed 10377396Ref.4. Source: SGD U5 snRNP Inferred from direct assay PubMed 11720284. Source: SGD
Molecular_function	GTP binding Inferred from sequence or structural similarity Ref.7. Source: SGD GTPase activity Inferred from mutant phenotype Ref.7. Source: SGD U5 snRNA binding Inferred from direct assay PubMed 9848662. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1008	1008	Pre-mRNA-splicing factor SNU114		PRO_0000091565
Regions
	Nucleotide binding	140 – 147	8	GTP By similarity
	Nucleotide binding	214 – 218	5	GTP By similarity
	Nucleotide binding	268 – 271	4	GTP By similarity
Amino acid modifications
	Modified residue	85	1	Phosphoserine Ref.9 Ref.11
	Modified residue	88	1	Phosphothreonine Ref.10 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P36048 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5CE30CEFF154D013	FASTA	1,008	114,041
	10 20 30 40 50 60 MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK 70 80 90 100 110 120 ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL QEHTIFTQLK KNIPKTRYNR 130 140 150 160 170 180 DYMLSMANIP ERIINVGVIG PLHSGKTSLM DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN 190 200 210 220 230 240 LKQEIDRGLS IKLNGSTLLC TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID 250 260 270 280 290 300 VVEGVTFVVE QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK 310 320 330 340 350 360 GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG SVYYHKGNFR 370 380 390 400 410 420 TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL RSNFRVNLSQ EALQYDPQPF 430 440 450 460 470 480 LKHVLQLIFR QQTGLVDAIT RCYQPFELFD NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG 490 500 510 520 530 540 AEWSLVRIYS GLLKRGDTVR ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE 550 560 570 580 590 600 EIGLLGGRYV YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE 610 620 630 640 650 660 AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL YMDCLLYDLR 670 680 690 700 710 720 ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE ENLPGLSISV AAEPMDSKMI 730 740 750 760 770 780 QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI LRTEYGWDSL ASRNVWSFYN GNVLINDTLP 790 800 810 820 830 840 DEISPELLSK YKEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP 850 860 870 880 890 900 LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL 910 920 930 940 950 960 EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF IPKLKPAPIN 970 980 990 1000 SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ LRENGLVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP." Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P. EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, MASS SPECTROMETRY.
[5]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[6]	Akada R., Yamamoto J., Yamashita I. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 823-846. Strain: ATCC 26786 / X2180-1A.
[7]	"An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2." Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R. EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, MASS SPECTROMETRY. Strain: ADR376.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND THR-88, MASS SPECTROMETRY.
[12]	"Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy." Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R. Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.
[13]	"Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome." Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D. RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CWC21 AND PRP8.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26878 Genomic DNA. Translation: CAA81514.1. Z28173 Genomic DNA. Translation: CAA82015.1. D28149 Genomic DNA. Translation: BAA05682.1. BK006944 Genomic DNA. Translation: DAA08993.1.
PIR	S38003.
RefSeq	NP_012748.1. NM_001179739.1.
3D structure databases
ProteinModelPortal	P36048.
SMR	P36048. Positions 132-283, 598-947.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-759N.
IntAct	P36048. 47 interactions.
MINT	MINT-426147.
STRING	4932.YKL173W.
Proteomic databases
PaxDb	P36048.
PeptideAtlas	P36048.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL173W; YKL173W; YKL173W.
GeneID	853681.
KEGG	sce:YKL173W.
Organism-specific databases
CYGD	YKL173w.
SGD	S000001656. SNU114.
Phylogenomic databases
eggNOG	COG0480.
GeneTree	ENSGT00660000096693.
HOGENOM	HOG000231589.
KO	K12853.
OMA	YGWDSLA.
OrthoDB	EOG44TSH2.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31940-MONOMER.
Gene expression databases
Genevestigator	P36048.
GermOnline	YKL173W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.230.10. 1 hit. 3.30.70.240. 1 hit.
InterPro	IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR027417. P-loop_NTPase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF52540. SSF52540. 1 hit. SSF50447. Translat_factor. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	974643.

Entry information

Entry name

SN114_YEAST

Accession

Primary (citable) accession number: P36048
Secondary accession number(s): D6VX27

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program