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Protein

Pre-mRNA-splicing factor SNU114

Gene

SNU114

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 147GTPBy similarity8
Nucleotide bindingi214 – 218GTPBy similarity5
Nucleotide bindingi268 – 271GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • U5 snRNA binding Source: SGD

GO - Biological processi

  • generation of catalytic spliceosome for first transesterification step Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • spliceosomal tri-snRNP complex assembly Source: SGD
  • spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31940-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SNU114
Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component
Growth inhibitory protein 10
Gene namesi
Name:SNU114
Synonyms:GIN10
Ordered Locus Names:YKL173W
ORF Names:YKL637
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL173W.
SGDiS000001656. SNU114.

Subcellular locationi

GO - Cellular componenti

  • U4/U6 x U5 tri-snRNP complex Source: SGD
  • U5 snRNP Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915651 – 1008Pre-mRNA-splicing factor SNU114Add BLAST1008

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphoserineCombined sources1
Modified residuei88PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36048.
PRIDEiP36048.

PTM databases

iPTMnetiP36048.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Interacts (via C-terminus) with CWC21. Interacts (via N-terminus) with PRP8 (via SCwid domain).4 Publications

Protein-protein interaction databases

BioGridi33965. 410 interactors.
DIPiDIP-759N.
IntActiP36048. 48 interactors.
MINTiMINT-426147.
STRINGi4932.YKL173W.

Structurei

Secondary structure

11008
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni68 – 70Combined sources3
Beta strandi75 – 77Combined sources3
Turni101 – 103Combined sources3
Beta strandi110 – 113Combined sources4
Helixi120 – 126Combined sources7
Turni130 – 132Combined sources3
Beta strandi134 – 139Combined sources6
Helixi146 – 157Combined sources12
Helixi166 – 169Combined sources4
Helixi182 – 186Combined sources5
Beta strandi194 – 201Combined sources8
Beta strandi207 – 214Combined sources8
Helixi222 – 231Combined sources10
Beta strandi233 – 240Combined sources8
Turni241 – 243Combined sources3
Helixi247 – 258Combined sources12
Beta strandi262 – 268Combined sources7
Helixi271 – 274Combined sources4
Turni275 – 277Combined sources3
Helixi281 – 296Combined sources16
Helixi306 – 308Combined sources3
Beta strandi311 – 315Combined sources5
Turni316 – 319Combined sources4
Beta strandi320 – 322Combined sources3
Helixi324 – 330Combined sources7
Helixi332 – 335Combined sources4
Helixi338 – 340Combined sources3
Helixi341 – 346Combined sources6
Beta strandi349 – 351Combined sources3
Beta strandi356 – 360Combined sources5
Beta strandi362 – 364Combined sources3
Helixi372 – 376Combined sources5
Helixi378 – 390Combined sources13
Helixi393 – 402Combined sources10
Turni410 – 413Combined sources4
Helixi417 – 428Combined sources12
Helixi433 – 440Combined sources8
Helixi445 – 455Combined sources11
Beta strandi465 – 476Combined sources12
Beta strandi479 – 481Combined sources3
Beta strandi483 – 491Combined sources9
Beta strandi499 – 503Combined sources5
Helixi504 – 506Combined sources3
Beta strandi541 – 545Combined sources5
Beta strandi547 – 554Combined sources8
Beta strandi561 – 566Combined sources6
Helixi568 – 570Combined sources3
Beta strandi575 – 579Combined sources5
Helixi583 – 588Combined sources6
Beta strandi603 – 611Combined sources9
Helixi612 – 614Combined sources3
Helixi615 – 628Combined sources14
Beta strandi633 – 636Combined sources4
Beta strandi642 – 648Combined sources7
Helixi649 – 661Combined sources13
Beta strandi668 – 670Combined sources3
Beta strandi678 – 681Combined sources4
Beta strandi708 – 714Combined sources7
Helixi717 – 723Combined sources7
Helixi737 – 739Combined sources3
Helixi744 – 755Combined sources12
Helixi759 – 764Combined sources6
Beta strandi772 – 776Combined sources5
Helixi779 – 783Combined sources5
Helixi785 – 803Combined sources19
Beta strandi807 – 810Combined sources4
Beta strandi815 – 823Combined sources9
Helixi833 – 836Combined sources4
Helixi838 – 851Combined sources14
Beta strandi855 – 869Combined sources15
Helixi870 – 872Combined sources3
Helixi873 – 883Combined sources11
Beta strandi887 – 893Combined sources7
Beta strandi897 – 908Combined sources12
Helixi909 – 911Combined sources3
Helixi914 – 921Combined sources8
Turni922 – 924Combined sources3
Beta strandi928 – 931Combined sources4
Beta strandi937 – 939Combined sources3
Beta strandi953 – 955Combined sources3
Helixi959 – 961Combined sources3
Helixi963 – 974Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80H1-1008[»]
5GAMelectron microscopy3.70C1-1008[»]
5GANelectron microscopy3.60C1-1008[»]
5GM6electron microscopy3.50C1-1008[»]
5GMKelectron microscopy3.40C1-1008[»]
5LJ3electron microscopy3.80C1-1008[»]
5LJ5electron microscopy3.80C1-1008[»]
5LQWelectron microscopy5.80B1-1008[»]
5MPSelectron microscopy3.85C1-1008[»]
5MQ0electron microscopy4.17C1-1008[»]
5NRLelectron microscopy7.20C1-1008[»]
5WSGelectron microscopy4.00C1-1008[»]
ProteinModelPortaliP36048.
SMRiP36048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 338tr-type GPROSITE-ProRule annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 147G1PROSITE-ProRule annotation8
Regioni188 – 192G2PROSITE-ProRule annotation5
Regioni214 – 217G3PROSITE-ProRule annotation4
Regioni268 – 271G4PROSITE-ProRule annotation4
Regioni315 – 317G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000231589.
InParanoidiP36048.
KOiK12853.
OMAiCFTLRSF.
OrthoDBiEOG092C0D4V.

Family and domain databases

CDDicd04098. eEF2_C_snRNP. 1 hit.
Gene3Di3.30.230.10. 1 hit.
InterProiView protein in InterPro
IPR035647. EFG_III/V.
IPR000640. EFG_V-like.
IPR004161. EFTu-like_2.
IPR031950. EFTUD2_N.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR035655. U5-116kD_C.
PfamiView protein in Pfam
PF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF16004. EFTUD2. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SMARTiView protein in SMART
SM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
PROSITEiView protein in PROSITE
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P36048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES
60 70 80 90 100
RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL
110 120 130 140 150
QEHTIFTQLK KNIPKTRYNR DYMLSMANIP ERIINVGVIG PLHSGKTSLM
160 170 180 190 200
DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN LKQEIDRGLS IKLNGSTLLC
210 220 230 240 250
TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID VVEGVTFVVE
260 270 280 290 300
QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK
310 320 330 340 350
GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG
360 370 380 390 400
SVYYHKGNFR TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL
410 420 430 440 450
RSNFRVNLSQ EALQYDPQPF LKHVLQLIFR QQTGLVDAIT RCYQPFELFD
460 470 480 490 500
NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG AEWSLVRIYS GLLKRGDTVR
510 520 530 540 550
ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE EIGLLGGRYV
560 570 580 590 600
YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE
610 620 630 640 650
AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL
660 670 680 690 700
YMDCLLYDLR ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE
710 720 730 740 750
ENLPGLSISV AAEPMDSKMI QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI
760 770 780 790 800
LRTEYGWDSL ASRNVWSFYN GNVLINDTLP DEISPELLSK YKEQIIQGFY
810 820 830 840 850
WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL
860 870 880 890 900
LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL
910 920 930 940 950
EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF
960 970 980 990 1000
IPKLKPAPIN SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ

LRENGLVP
Length:1,008
Mass (Da):114,041
Last modified:June 1, 1994 - v1
Checksum:i5CE30CEFF154D013
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81514.1.
Z28173 Genomic DNA. Translation: CAA82015.1.
D28149 Genomic DNA. Translation: BAA05682.1.
BK006944 Genomic DNA. Translation: DAA08993.1.
PIRiS38003.
RefSeqiNP_012748.1. NM_001179739.1.

Genome annotation databases

EnsemblFungiiYKL173W; YKL173W; YKL173W.
GeneIDi853681.
KEGGisce:YKL173W.

Similar proteinsi

Entry informationi

Entry nameiSN114_YEAST
AccessioniPrimary (citable) accession number: P36048
Secondary accession number(s): D6VX27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: August 30, 2017
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names