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P36048

- SN114_YEAST

UniProt

P36048 - SN114_YEAST

Protein

Pre-mRNA-splicing factor SNU114

Gene

SNU114

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi140 – 1478GTPBy similarity
    Nucleotide bindingi214 – 2185GTPBy similarity
    Nucleotide bindingi268 – 2714GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: SGD
    3. U5 snRNA binding Source: SGD

    GO - Biological processi

    1. generation of catalytic spliceosome for first transesterification step Source: SGD
    2. GTP catabolic process Source: GOC
    3. mRNA splicing, via spliceosome Source: SGD
    4. spliceosomal tri-snRNP complex assembly Source: SGD
    5. spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31940-MONOMER.
    ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor SNU114
    Alternative name(s):
    114 kDa U5 small nuclear ribonucleoprotein component
    Growth inhibitory protein 10
    Gene namesi
    Name:SNU114
    Synonyms:GIN10
    Ordered Locus Names:YKL173W
    ORF Names:YKL637
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL173w.
    SGDiS000001656. SNU114.

    Subcellular locationi

    GO - Cellular componenti

    1. U4/U6 x U5 tri-snRNP complex Source: SGD
    2. U5 snRNP Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10081008Pre-mRNA-splicing factor SNU114PRO_0000091565Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei85 – 851Phosphoserine1 Publication
    Modified residuei88 – 881Phosphothreonine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36048.
    PaxDbiP36048.
    PeptideAtlasiP36048.

    Expressioni

    Gene expression databases

    GenevestigatoriP36048.

    Interactioni

    Subunit structurei

    Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Interacts (via C-terminus) with CWC21. Interacts (via N-terminus) with PRP8 (via SCwid domain).4 Publications

    Protein-protein interaction databases

    BioGridi33965. 133 interactions.
    DIPiDIP-759N.
    IntActiP36048. 48 interactions.
    MINTiMINT-426147.
    STRINGi4932.YKL173W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36048.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini131 – 338208tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni140 – 1478G1PROSITE-ProRule annotation
    Regioni188 – 1925G2PROSITE-ProRule annotation
    Regioni214 – 2174G3PROSITE-ProRule annotation
    Regioni268 – 2714G4PROSITE-ProRule annotation
    Regioni315 – 3173G5PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0480.
    HOGENOMiHOG000231589.
    KOiK12853.
    OMAiYGWDSLA.
    OrthoDBiEOG7966SQ.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    PROSITEiPS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36048-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES     50
    RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL 100
    QEHTIFTQLK KNIPKTRYNR DYMLSMANIP ERIINVGVIG PLHSGKTSLM 150
    DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN LKQEIDRGLS IKLNGSTLLC 200
    TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID VVEGVTFVVE 250
    QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK 300
    GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG 350
    SVYYHKGNFR TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL 400
    RSNFRVNLSQ EALQYDPQPF LKHVLQLIFR QQTGLVDAIT RCYQPFELFD 450
    NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG AEWSLVRIYS GLLKRGDTVR 500
    ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE EIGLLGGRYV 550
    YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE 600
    AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL 650
    YMDCLLYDLR ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE 700
    ENLPGLSISV AAEPMDSKMI QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI 750
    LRTEYGWDSL ASRNVWSFYN GNVLINDTLP DEISPELLSK YKEQIIQGFY 800
    WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL 850
    LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL 900
    EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF 950
    IPKLKPAPIN SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ 1000
    LRENGLVP 1008
    Length:1,008
    Mass (Da):114,041
    Last modified:June 1, 1994 - v1
    Checksum:i5CE30CEFF154D013
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26878 Genomic DNA. Translation: CAA81514.1.
    Z28173 Genomic DNA. Translation: CAA82015.1.
    D28149 Genomic DNA. Translation: BAA05682.1.
    BK006944 Genomic DNA. Translation: DAA08993.1.
    PIRiS38003.
    RefSeqiNP_012748.1. NM_001179739.1.

    Genome annotation databases

    EnsemblFungiiYKL173W; YKL173W; YKL173W.
    GeneIDi853681.
    KEGGisce:YKL173W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26878 Genomic DNA. Translation: CAA81514.1 .
    Z28173 Genomic DNA. Translation: CAA82015.1 .
    D28149 Genomic DNA. Translation: BAA05682.1 .
    BK006944 Genomic DNA. Translation: DAA08993.1 .
    PIRi S38003.
    RefSeqi NP_012748.1. NM_001179739.1.

    3D structure databases

    ProteinModelPortali P36048.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33965. 133 interactions.
    DIPi DIP-759N.
    IntActi P36048. 48 interactions.
    MINTi MINT-426147.
    STRINGi 4932.YKL173W.

    Proteomic databases

    MaxQBi P36048.
    PaxDbi P36048.
    PeptideAtlasi P36048.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL173W ; YKL173W ; YKL173W .
    GeneIDi 853681.
    KEGGi sce:YKL173W.

    Organism-specific databases

    CYGDi YKL173w.
    SGDi S000001656. SNU114.

    Phylogenomic databases

    eggNOGi COG0480.
    HOGENOMi HOG000231589.
    KOi K12853.
    OMAi YGWDSLA.
    OrthoDBi EOG7966SQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31940-MONOMER.
    Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

    Miscellaneous databases

    NextBioi 974643.

    Gene expression databases

    Genevestigatori P36048.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR009000. Transl_B-barrel.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    PROSITEi PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
      Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
      Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
      EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Akada R., Yamamoto J., Yamashita I.
      Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 823-846.
      Strain: ATCC 26786 / X2180-1A.
    7. "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2."
      Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R.
      EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
      Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
      Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.
    12. "Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."
      Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D.
      RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CWC21 AND PRP8.
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSN114_YEAST
    AccessioniPrimary (citable) accession number: P36048
    Secondary accession number(s): D6VX27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3