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Protein

Pre-mRNA-splicing factor SNU114

Gene

SNU114

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 147GTPBy similarity8
Nucleotide bindingi214 – 218GTPBy similarity5
Nucleotide bindingi268 – 271GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • U5 snRNA binding Source: SGD

GO - Biological processi

  • generation of catalytic spliceosome for first transesterification step Source: SGD
  • mature ribosome assembly Source: GO_Central
  • mRNA splicing, via spliceosome Source: SGD
  • spliceosomal tri-snRNP complex assembly Source: SGD
  • spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31940-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SNU114
Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component
Growth inhibitory protein 10
Gene namesi
Name:SNU114
Synonyms:GIN10
Ordered Locus Names:YKL173W
ORF Names:YKL637
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL173W.
SGDiS000001656. SNU114.

Subcellular locationi

GO - Cellular componenti

  • U4/U6 x U5 tri-snRNP complex Source: SGD
  • U5 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915651 – 1008Pre-mRNA-splicing factor SNU114Add BLAST1008

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphoserineCombined sources1
Modified residuei88PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36048.
PRIDEiP36048.

PTM databases

iPTMnetiP36048.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Interacts (via C-terminus) with CWC21. Interacts (via N-terminus) with PRP8 (via SCwid domain).4 Publications

Protein-protein interaction databases

BioGridi33965. 125 interactors.
DIPiDIP-759N.
IntActiP36048. 48 interactors.
MINTiMINT-426147.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80H1-1008[»]
5GAMelectron microscopy3.70C1-1008[»]
5GANelectron microscopy3.60C1-1008[»]
5GM6electron microscopy3.50C1-1008[»]
5GMKelectron microscopy3.40C1-1008[»]
5LJ3electron microscopy3.80C1-1008[»]
5LJ5electron microscopy3.80C1-1008[»]
5LQWelectron microscopy5.80B1-1008[»]
ProteinModelPortaliP36048.
SMRiP36048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 338tr-type GPROSITE-ProRule annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 147G1PROSITE-ProRule annotation8
Regioni188 – 192G2PROSITE-ProRule annotation5
Regioni214 – 217G3PROSITE-ProRule annotation4
Regioni268 – 271G4PROSITE-ProRule annotation4
Regioni315 – 317G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000135290.
HOGENOMiHOG000231589.
InParanoidiP36048.
KOiK12853.
OMAiLSRDFVM.
OrthoDBiEOG092C0D4V.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031950. EFTUD2_N.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF16004. EFTUD2. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES
60 70 80 90 100
RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL
110 120 130 140 150
QEHTIFTQLK KNIPKTRYNR DYMLSMANIP ERIINVGVIG PLHSGKTSLM
160 170 180 190 200
DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN LKQEIDRGLS IKLNGSTLLC
210 220 230 240 250
TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID VVEGVTFVVE
260 270 280 290 300
QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK
310 320 330 340 350
GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG
360 370 380 390 400
SVYYHKGNFR TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL
410 420 430 440 450
RSNFRVNLSQ EALQYDPQPF LKHVLQLIFR QQTGLVDAIT RCYQPFELFD
460 470 480 490 500
NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG AEWSLVRIYS GLLKRGDTVR
510 520 530 540 550
ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE EIGLLGGRYV
560 570 580 590 600
YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE
610 620 630 640 650
AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL
660 670 680 690 700
YMDCLLYDLR ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE
710 720 730 740 750
ENLPGLSISV AAEPMDSKMI QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI
760 770 780 790 800
LRTEYGWDSL ASRNVWSFYN GNVLINDTLP DEISPELLSK YKEQIIQGFY
810 820 830 840 850
WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL
860 870 880 890 900
LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL
910 920 930 940 950
EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF
960 970 980 990 1000
IPKLKPAPIN SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ

LRENGLVP
Length:1,008
Mass (Da):114,041
Last modified:June 1, 1994 - v1
Checksum:i5CE30CEFF154D013
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81514.1.
Z28173 Genomic DNA. Translation: CAA82015.1.
D28149 Genomic DNA. Translation: BAA05682.1.
BK006944 Genomic DNA. Translation: DAA08993.1.
PIRiS38003.
RefSeqiNP_012748.1. NM_001179739.1.

Genome annotation databases

EnsemblFungiiYKL173W; YKL173W; YKL173W.
GeneIDi853681.
KEGGisce:YKL173W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81514.1.
Z28173 Genomic DNA. Translation: CAA82015.1.
D28149 Genomic DNA. Translation: BAA05682.1.
BK006944 Genomic DNA. Translation: DAA08993.1.
PIRiS38003.
RefSeqiNP_012748.1. NM_001179739.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80H1-1008[»]
5GAMelectron microscopy3.70C1-1008[»]
5GANelectron microscopy3.60C1-1008[»]
5GM6electron microscopy3.50C1-1008[»]
5GMKelectron microscopy3.40C1-1008[»]
5LJ3electron microscopy3.80C1-1008[»]
5LJ5electron microscopy3.80C1-1008[»]
5LQWelectron microscopy5.80B1-1008[»]
ProteinModelPortaliP36048.
SMRiP36048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33965. 125 interactors.
DIPiDIP-759N.
IntActiP36048. 48 interactors.
MINTiMINT-426147.

PTM databases

iPTMnetiP36048.

Proteomic databases

MaxQBiP36048.
PRIDEiP36048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL173W; YKL173W; YKL173W.
GeneIDi853681.
KEGGisce:YKL173W.

Organism-specific databases

EuPathDBiFungiDB:YKL173W.
SGDiS000001656. SNU114.

Phylogenomic databases

GeneTreeiENSGT00860000135290.
HOGENOMiHOG000231589.
InParanoidiP36048.
KOiK12853.
OMAiLSRDFVM.
OrthoDBiEOG092C0D4V.

Enzyme and pathway databases

BioCyciYEAST:G3O-31940-MONOMER.

Miscellaneous databases

PROiP36048.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031950. EFTUD2_N.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF16004. EFTUD2. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSN114_YEAST
AccessioniPrimary (citable) accession number: P36048
Secondary accession number(s): D6VX27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.