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P36048 (SN114_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor SNU114
Alternative name(s):
114 kDa U5 small nuclear ribonucleoprotein component
Growth inhibitory protein 10
Gene names
Name:SNU114
Synonyms:GIN10
Ordered Locus Names:YKL173W
ORF Names:YKL637
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1008 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.

Subunit structure

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Interacts (via C-terminus) with CWC21. Interacts (via N-terminus) with PRP8 (via SCwid domain). Ref.4 Ref.5 Ref.11 Ref.12

Subcellular location

Nucleus.

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10081008Pre-mRNA-splicing factor SNU114
PRO_0000091565

Regions

Nucleotide binding140 – 1478GTP By similarity
Nucleotide binding214 – 2185GTP By similarity
Nucleotide binding268 – 2714GTP By similarity

Amino acid modifications

Modified residue851Phosphoserine Ref.13
Modified residue881Phosphothreonine Ref.9 Ref.10 Ref.13

Sequences

Sequence LengthMass (Da)Tools
P36048 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 5CE30CEFF154D013

FASTA1,008114,041
        10         20         30         40         50         60 
MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK 

        70         80         90        100        110        120 
ELGISLEHPY GKEVEVLMET KNTQSPQTPL VEPVTERTKL QEHTIFTQLK KNIPKTRYNR 

       130        140        150        160        170        180 
DYMLSMANIP ERIINVGVIG PLHSGKTSLM DLLVIDSHKR IPDMSKNVEL GWKPLRYLDN 

       190        200        210        220        230        240 
LKQEIDRGLS IKLNGSTLLC TDLESKSRMI NFLDAPGHVN FMDETAVALA ASDLVLIVID 

       250        260        270        280        290        300 
VVEGVTFVVE QLIKQSIKNN VAMCFVINKL DRLILDLKLP PMDAYLKLNH IIANINSFTK 

       310        320        330        340        350        360 
GNVFSPIDNN IIFASTKLGF TFTIKEFVSY YYAHSIPSSK IDDFTTRLWG SVYYHKGNFR 

       370        380        390        400        410        420 
TKPFENVEKY PTFVEFILIP LYKIFSYALS MEKDKLKNLL RSNFRVNLSQ EALQYDPQPF 

       430        440        450        460        470        480 
LKHVLQLIFR QQTGLVDAIT RCYQPFELFD NKTAHLSIPG KSTPEGTLWA HVLKTVDYGG 

       490        500        510        520        530        540 
AEWSLVRIYS GLLKRGDTVR ILDTSQSESR QKRQLHDISK TETSNEDEDE DDETPSCEVE 

       550        560        570        580        590        600 
EIGLLGGRYV YPVHEAHKGQ IVLIKGISSA YIKSATLYSV KSKEDMKQLK FFKPLDYITE 

       610        620        630        640        650        660 
AVFKIVLQPL LPRELPKLLD ALNKISKYYP GVIIKVEESG EHVILGNGEL YMDCLLYDLR 

       670        680        690        700        710        720 
ASYAKIEIKI SDPLTVFSES CSNESFASIP VSNSISRLGE ENLPGLSISV AAEPMDSKMI 

       730        740        750        760        770        780 
QDLSRNTLGK GQNCLDIDGI MDNPRKLSKI LRTEYGWDSL ASRNVWSFYN GNVLINDTLP 

       790        800        810        820        830        840 
DEISPELLSK YKEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP 

       850        860        870        880        890        900 
LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIYK TIKVAGTPLL 

       910        920        930        940        950        960 
EVRGQVPVIE SAGFETDLRL STNGLGMCQL YFWHKIWRKV PGDVLDKDAF IPKLKPAPIN 

       970        980        990       1000 
SLSRDFVMKT RRRKGISTGG FMSNDGPTLE KYISAELYAQ LRENGLVP 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, MASS SPECTROMETRY.
[5]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[6]Akada R., Yamamoto J., Yamashita I.
Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 823-846.
Strain: ATCC 26786 / X2180-1A.
[7]"An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2."
Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R.
EMBO J. 16:4092-4106(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.
[12]"Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."
Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D.
RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CWC21 AND PRP8.
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND THR-88, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z26878 Genomic DNA. Translation: CAA81514.1.
Z28173 Genomic DNA. Translation: CAA82015.1.
D28149 Genomic DNA. Translation: BAA05682.1.
BK006944 Genomic DNA. Translation: DAA08993.1.
PIRS38003.
RefSeqNP_012748.1. NM_001179739.1.

3D structure databases

ProteinModelPortalP36048.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33965. 133 interactions.
DIPDIP-759N.
IntActP36048. 48 interactions.
MINTMINT-426147.
STRING4932.YKL173W.

Proteomic databases

PaxDbP36048.
PeptideAtlasP36048.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL173W; YKL173W; YKL173W.
GeneID853681.
KEGGsce:YKL173W.

Organism-specific databases

CYGDYKL173w.
SGDS000001656. SNU114.

Phylogenomic databases

eggNOGCOG0480.
GeneTreeENSGT00740000116702.
HOGENOMHOG000231589.
KOK12853.
OMALSRDFVM.
OrthoDBEOG7966SQ.

Enzyme and pathway databases

BioCycYEAST:G3O-31940-MONOMER.

Gene expression databases

GenevestigatorP36048.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
ProtoNetSearch...

Other

NextBio974643.

Entry information

Entry nameSN114_YEAST
AccessionPrimary (citable) accession number: P36048
Secondary accession number(s): D6VX27
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 19, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families