P36047 (SDS22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit SDS22

Gene names

Name:	SDS22
Synonyms:	EGP1
Ordered Locus Names:	YKL193C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potential regulator of the mitotic function of yeast type 1 protein phosphatase.
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the SDS22 family. Contains 11 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Domain	Leucine-rich repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from mutant phenotype. Source: SGD maintenance of protein location in nucleus Inferred from mutant phenotype. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase type 1 regulator activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GLC7	P32598	10	EBI-16783,EBI-13715
PPQ1	P32945	3	EBI-16783,EBI-13787
YPI1	P43587	4	EBI-16783,EBI-22913

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Protein phosphatase 1 regulatory subunit SDS22		PRO_0000071447
Regions
	Repeat	43 – 64	22	LRR 1
	Repeat	67 – 88	22	LRR 2
	Repeat	91 – 113	23	LRR 3
	Repeat	114 – 135	22	LRR 4
	Repeat	136 – 157	22	LRR 5
	Repeat	158 – 179	22	LRR 6
	Repeat	182 – 203	22	LRR 7
	Repeat	204 – 225	22	LRR 8
	Repeat	226 – 247	22	LRR 9
	Repeat	248 – 269	22	LRR 10
	Repeat	270 – 292	23	LRR 11
	Domain	306 – 338	33	LRRCT
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P36047 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 1EF434C7276ABAEA	FASTA	338	38,887
	10 20 30 40 50 60 MDKNSVNKDS EEKDERHKIE VVDDTNPDFI TADSELTQDL PDDVEVIDLV HLKIKSLEDL 70 80 90 100 110 120 NLYRFKNLKQ LCLRQNLIES ISEVEVLPHD KIVDLDFYDN KIKHISSNVN KLTKLTSLDL 130 140 150 160 170 180 SFNKIKHIKN LENLTDLENL YFVQNSISKI ENLSTLKSLK NLELGGNKVH SIEPDSFEGL 190 200 210 220 230 240 SNLEEIWLGK NSIPRLINLH PLKNLKILSI QSNKLKKIEN LEELTNLEEL YLSHNFITKI 250 260 270 280 290 300 EGLEKNLKLT TLDVTSNKIT SLENLNHLSN LTDIWASFNK IDQSFESLGE NLSALSRLET 310 320 330 IYLEGNPIQL ENKTSYRRKL TMNLPPSLQK IDATYIRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase." Mackelvie S.H., Andrews P.D., Stark M.J.R. Mol. Cell. Biol. 15:3777-3785(1995) [PubMed: 7791785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200358 / YNN 295.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae." Cheret G., Mattheakis L.C., Sor F. Yeast 9:661-667(1993) [PubMed: 8394042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-136. Strain: S288c / GRF88.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83609 Genomic DNA. Translation: CAA58588.1. Z28193 Genomic DNA. Translation: CAA82037.1. AY558314 Genomic DNA. Translation: AAS56640.1. X69765 Genomic DNA. No translation available. BK006944 Genomic DNA. Translation: DAA08973.1.
PIR	S38030.
RefSeq	NP_012728.1. NM_001179759.1.
3D structure databases
ProteinModelPortal	P36047.
SMR	P36047. Positions 15-336.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2378N.
IntAct	P36047. 28 interactions.
MINT	MINT-641828.
STRING	P36047.
Proteomic databases
PeptideAtlas	P36047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL193C; YKL193C; YKL193C.
GeneID	853641.
KEGG	sce:YKL193C.
NMPDR	fig|4932.3.peg.3705.
Organism-specific databases
CYGD	YKL193c.
SGD	S000001676. SDS22.
Phylogenomic databases
eggNOG	fuNOG06178.
GeneTree	EFGT00050000002537.
HOGENOM	HBG526095.
OMA	DATFVRF.
OrthoDB	EOG4B01ZB.
Gene expression databases
ArrayExpress	P36047.
Genevestigator	P36047.
GermOnline	YKL193C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. [Graphical view]
PROSITE	PS51450. LRR. 12 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	974534.

Entry information

Entry name

SDS22_YEAST

Accession

Primary (citable) accession number: P36047
Secondary accession number(s): D6VX07

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program