Reviewed, UniProtKB/Swiss-Prot P36044 (MNN4_YEAST)

Names and origin

Protein names

Recommended name:
    Protein MNN4

Gene names

Name:	MNN4
Ordered Locus Names:	YKL201C/YKL200C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function as a positive regulator for mannosylphosphate transferase. Is required to mediate mannosylphosphate transfer in both the core and outer chain portions of N-linked oligosaccharides.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Sequence similarities	To yeast YJR061w.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal-anchor Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Ref.1 Inferred from mutant phenotype. Source: SGD protein amino acid O-linked glycosylation Inferred from mutant phenotype. Source: SGD response to stress Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1178	1178	Protein MNN4		PRO_0000096525
Regions
	Topological domain	1 – 27	27	Cytoplasmic Potential
	Transmembrane	28 – 48	21	Signal-anchor for type II membrane protein Potential
	Topological domain	49 – 1178	1130	Lumenal Potential
	Repeat	1042 – 1049	8	1
	Repeat	1050 – 1057	8	2
	Repeat	1058 – 1065	8	3
	Repeat	1066 – 1073	8	4
	Repeat	1074 – 1081	8	5
	Repeat	1082 – 1089	8	6
	Repeat	1090 – 1097	8	7; approximate
	Repeat	1098 – 1105	8	8
	Repeat	1106 – 1113	8	9; approximate
	Repeat	1114 – 1121	8	10; approximate
	Repeat	1122 – 1129	8	11; approximate
	Repeat	1130 – 1137	8	12
	Repeat	1138 – 1144	7	13; approximate
	Repeat	1145 – 1152	8	14; approximate
	Repeat	1153 – 1160	8	15; approximate
	Repeat	1161 – 1168	8	16; approximate
	Repeat	1169 – 1174	6	17; truncated
	Region	1042 – 1174	133	17 X 8 AA tandem repeats of K-K-K-K-E-E-E-E
	Compositional bias	37 – 40	4	Poly-Ile
	Compositional bias	1032 – 1174	143	Arg/Glu/Lys-rich (highly charged)
	Compositional bias	1042 – 1045	4	Poly-Lys
	Compositional bias	1046 – 1049	4	Poly-Glu
	Compositional bias	1050 – 1053	4	Poly-Lys
	Compositional bias	1054 – 1057	4	Poly-Glu
	Compositional bias	1058 – 1061	4	Poly-Lys
	Compositional bias	1062 – 1065	4	Poly-Glu
	Compositional bias	1066 – 1069	4	Poly-Lys
	Compositional bias	1070 – 1073	4	Poly-Glu
	Compositional bias	1074 – 1077	4	Poly-Lys
	Compositional bias	1078 – 1081	4	Poly-Glu
	Compositional bias	1082 – 1085	4	Poly-Lys
	Compositional bias	1086 – 1089	4	Poly-Glu
	Compositional bias	1094 – 1097	4	Poly-Glu
	Compositional bias	1098 – 1101	4	Poly-Lys
	Compositional bias	1102 – 1105	4	Poly-Glu
	Compositional bias	1134 – 1137	4	Poly-Glu
	Compositional bias	1157 – 1160	4	Poly-Glu
	Compositional bias	1165 – 1168	4	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	P36044-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: BC05DAE0AEFCB282	FASTA	1,178	139,381
	10 20 30 40 50 60 MLQRISSKLH RRFLSGLLRV KHYPLRRILL PLILLQIIII TFIWSNSPQR NGLGRDADYL 70 80 90 100 110 120 LPNYNELDSD DDSWYSILTS SFKNDRKIQF AKTLYENLKF GTNPKWVNEY TLQNDLLSVK 130 140 150 160 170 180 MGPRKGSKLE SVDELKFYDF DPRLTWSVVL NHLQNNDADQ PEKLPFSWYD WTTFHELNKL 190 200 210 220 230 240 ISIDKTVLPC NFLFQSAFDK ESLEAIETEL GEPLFLYERP KYAQKLWYKA ARNQDRIKDS 250 260 270 280 290 300 KELKKHCSKL FTPDGHGSPK GLRFNTQFQI KELYDKVRPE VYQLQARNYI LTTQSHPLSI 310 320 330 340 350 360 SIIESDNSTY QVPLQTEKSK NLVQSGLLQE YINDNINSTN KRKKNKQDVE FNHNRLFQEF 370 380 390 400 410 420 VNNDQVNSLY KLEIEETDKF TFDKDLVYLS PSDFKFDASK KIEELEEQKK LYPDKFSAHN 430 440 450 460 470 480 ENYLNSLKNS VKTSPALQRK FFYEAGAVKQ YKGMGFHRDK RFFNVDTLIN DKQEYQARLN 490 500 510 520 530 540 SMIRTFQKFT KANGIISWLS HGTLYGYLYN GMAFPWDNDF DLQMPIKHLQ LLSQYFNQSL 550 560 570 580 590 600 ILEDPRQGNG RYFLDVSDSL TVRINGNGKN NIDARFIDVD TGLYIDITGL ASTSAPSRDY 610 620 630 640 650 660 LNSYIEERLQ EEHLDINNIP ESNGETATLP DKVDDGLVNM ATLNITELRD YITSDENKNH 670 680 690 700 710 720 KRVPTDTDLK DLLKKELEEL PKSKTIENKL NPKQRYFLNE KLKLYNCRNN HFNSFEELSP 730 740 750 760 770 780 LINTVFHGVP ALIPHRHTYC LHNEYHVPDR YAFDAYKNTA YLPEFRFWFD YDGLKKCSNI 790 800 810 820 830 840 NSWYPNIPSI NSWNPNLLKE ISSTKFESKL FDSNKVSEYS FKNLSMDDVR LIYKNIPKAG 850 860 870 880 890 900 FIEVFTNLYN SFNVTAYRQK ELEIQYCQNL TFIEKKKLLH QLRINVAPKL SSPAKDPFLF 910 920 930 940 950 960 GYEKAMWKDL SKSMNQTTLD QVTKIVHEEY VGKIIDLSES LKYRNFSLFN ITFDETGTTL 970 980 990 1000 1010 1020 DDNTEDYTPA NTVEVNPVDF KSNLNFSSNS FLDLNSYGLD LFAPTLSDVN RKGIQMFDKD 1030 1040 1050 1060 1070 1080 PIIVYEDYAY AKLLEERKRR EKKKKEEEEK KKKEEEEKKK KEEEEKKKKE EEEKKKKEEE 1090 1100 1110 1120 1130 1140 EKKKKEEEEK KKQEEEEKKK KEEEEKKKQE EGEKMKNEDE ENKKNEDEEK KKNEEEEKKK 1150 1160 1170 QEEKNKKNED EEKKKQEEEE KKKNEEEEKK KQEEGHSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and analysis of the MNN4 gene required for phosphorylation of N-linked oligosaccharides in Saccharomyces cerevisiae." Odani T., Shimma Y., Tanaka A., Jigami Y. Glycobiology 6:805-810(1996) [PubMed: 9023541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D83006 Genomic DNA. Translation: BAA11676.1. Z28201 Genomic DNA. Translation: CAA82046.1. Z28200 Genomic DNA. Translation: CAA82044.1.
PIR	S78475.
RefSeq	NP_012721.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4199N.
IntAct	P36044. 4 interactions.
STRING	P36044.
Proteomic databases
PRIDE	P36044.
Genome annotation databases
Ensembl	YKL201C; YKL201C; YKL201C; Saccharomyces cerevisiae. [Genome view]
GeneID	853634.
KEGG	sce:YKL201C.
NMPDR	fig|4932.3.peg.3699.
Organism-specific databases
CYGD	YKL201c.
SGD	S000001684. MNN4.
Phylogenomic databases
HOGENOM	P36044.
OMA	MIRTFQK
OrthoDB	EOG9NZW9J
Gene expression databases
ArrayExpress	P36044.
Genevestigator	P36044.
GermOnline	YKL201C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007074. LicD-like. [Graphical view]
Pfam	PF04991. LicD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974514.

Entry information

Entry name

MNN4_YEAST

Accession

Primary (citable) accession number: P36044
Secondary accession number(s): P36043, P89095

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	December 15, 1998
Last modified:	November 24, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)