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P36041 (EAP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein EAP1
Alternative name(s):
eIF4E-associated protein 1
Gene names
Name:EAP1
Ordered Locus Names:YKL204W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length632 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can regulate translation through binding to eIF4E. Competes with eIF4G and p20 for binding to eIF4E in vivo and inhibits cap-dependent translation in vitro. Plays a role in cell growth and is implicated in the TOR signaling cascade. Functions independently of eIF4E to maintain genetic stability and to attenuate GCN4 translation upon TOR inactivation. Ref.3 Ref.4 Ref.7

Subunit structure

Interacts with SMY2, SYH1 and eIF4E. Ref.4 Ref.8

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 3240 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 632632Protein EAP1
PRO_0000203133

Regions

Nucleotide binding440 – 4478ATP Potential

Amino acid modifications

Modified residue301Phosphoserine Ref.10
Modified residue2811Phosphoserine Ref.10
Modified residue2821Phosphoserine Ref.10 Ref.11
Modified residue3271Phosphoserine Ref.10 Ref.11
Modified residue3441Phosphoserine Ref.10
Modified residue3871Phosphoserine Ref.10

Experimental info

Mutagenesis1091Y → A: Abolishes interaction with eIF4E; when associated with A-114. Ref.4 Ref.7
Mutagenesis1141L → A: Abolishes interaction with eIF4E; when associated with A-109. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P36041 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 775F73F57BB0EF2B

FASTA63269,762
        10         20         30         40         50         60 
MELNDPSIIS SSQFSGELSD SDTAAATHKS QQAISNLFQK LAKKGREEKP IGSVESSTDS 

        70         80         90        100        110        120 
SNISVATSGN NKESNKKKNK KTAMLNFSSL TDPITNYKPM DLQYKTYAYS MNELYHLKPS 

       130        140        150        160        170        180 
LASASYEEDP LISELVRSLP KRKFWRLRMG PPDQKHANNH HFNGNNGGGS WKAGYKNGKN 

       190        200        210        220        230        240 
DERRMSRTKN MQGGKRRSQQ DDEEKKIDQE MLEMDKNLQL GGDVGHSIAD FEDWKAKMKE 

       250        260        270        280        290        300 
LELKKLSKSK GISNSTAIAP RESASHETPT DLRPVIPRGP SSITDFLNLK RQDKKEESSQ 

       310        320        330        340        350        360 
QTPGIPVGQP SLSKTSIEQV NELETNSDLG KSSSSRFSSF FNKSATSLPS LDNNNQVPSS 

       370        380        390        400        410        420 
NVSVVNNDGN STPHQSGSRL MSFFKESRSS TPNAESQLLS ASDKDNGKMQ TLPQFQQQPQ 

       430        440        450        460        470        480 
QMQPMAFTQH PPNNNAFFNG LLNKGKSETS TPPPPPPGLI AHQGPQFPVM GVPPNFPQRM 

       490        500        510        520        530        540 
MPPPPGLVQF QKDSKDVNKK EDRQLRQNKN PNGTRNSKGK QEETATPDLP QQQYMPPPPP 

       550        560        570        580        590        600 
PGFFPMHPNF PNGPMPPLPQ GFPIPPNGML PVTGQQPQPP YPNMMLQGNF PPNFQQGFGS 

       610        620        630 
NSPMPIPSII NANGKNVTNQ LPPGLNSKKN IK 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability."
Chial H.J., Stemm-Wolf A.J., McBratney S., Winey M.
Curr. Biol. 10:1519-1522(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae."
Cosentino G.P., Schmelzle T., Haghighat A., Helliwell S.B., Hall M.N., Sonenberg N.
Mol. Cell. Biol. 20:4604-4613(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EIF4E, MUTAGENESIS OF TYR-109.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation."
Matsuo R., Kubota H., Obata T., Kito K., Ota K., Kitazono T., Ibayashi S., Sasaki T., Iida M., Ito T.
FEBS Lett. 579:2433-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TYR-109 AND LEU-114.
[8]"GYF domain proteomics reveals interaction sites in known and novel target proteins."
Kofler M., Motzny K., Freund C.
Mol. Cell. Proteomics 4:1797-1811(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SMY2 AND SYH1.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30; SER-281; SER-282; SER-327; SER-344 AND SER-387, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282 AND SER-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28204 Genomic DNA. Translation: CAA82049.1.
BK006944 Genomic DNA. Translation: DAA08965.1.
PIRS38042.
RefSeqNP_012718.1. NM_001179769.1.

3D structure databases

ProteinModelPortalP36041.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33919. 65 interactions.
DIPDIP-1778N.
IntActP36041. 11 interactions.
MINTMINT-394822.
STRING4932.YKL204W.

Proteomic databases

PaxDbP36041.
PeptideAtlasP36041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL204W; YKL204W; YKL204W.
GeneID853631.
KEGGsce:YKL204W.

Organism-specific databases

CYGDYKL204w.
SGDS000001687. EAP1.

Phylogenomic databases

eggNOGNOG12793.
KOK15908.
OMANDERRMS.
OrthoDBEOG7J18CB.

Enzyme and pathway databases

BioCycYEAST:G3O-31963-MONOMER.

Gene expression databases

GenevestigatorP36041.

Family and domain databases

ProtoNetSearch...

Other

NextBio974508.

Entry information

Entry nameEAP1_YEAST
AccessionPrimary (citable) accession number: P36041
Secondary accession number(s): D6VWZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD