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P36041

- EAP1_YEAST

UniProt

P36041 - EAP1_YEAST

Protein

Protein EAP1

Gene

EAP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Can regulate translation through binding to eIF4E. Competes with eIF4G and p20 for binding to eIF4E in vivo and inhibits cap-dependent translation in vitro. Plays a role in cell growth and is implicated in the TOR signaling cascade. Functions independently of eIF4E to maintain genetic stability and to attenuate GCN4 translation upon TOR inactivation.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi440 – 4478ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. eukaryotic initiation factor 4E binding Source: UniProtKB

    GO - Biological processi

    1. deadenylation-dependent decapping of nuclear-transcribed mRNA Source: SGD
    2. mRNA catabolic process Source: SGD
    3. negative regulation of translation Source: SGD
    4. regulation of growth Source: UniProtKB-KW

    Keywords - Biological processi

    Growth regulation, Translation regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31963-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein EAP1
    Alternative name(s):
    eIF4E-associated protein 1
    Gene namesi
    Name:EAP1
    Ordered Locus Names:YKL204W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL204w.
    SGDiS000001687. EAP1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasmic stress granule Source: SGD
    2. mRNA cap binding complex Source: SGD
    3. polysome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi109 – 1091Y → A: Abolishes interaction with eIF4E; when associated with A-114. 2 Publications
    Mutagenesisi114 – 1141L → A: Abolishes interaction with eIF4E; when associated with A-109. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 632632Protein EAP1PRO_0000203133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Phosphoserine1 Publication
    Modified residuei281 – 2811Phosphoserine1 Publication
    Modified residuei282 – 2821Phosphoserine2 Publications
    Modified residuei327 – 3271Phosphoserine2 Publications
    Modified residuei344 – 3441Phosphoserine1 Publication
    Modified residuei387 – 3871Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36041.
    PaxDbiP36041.
    PeptideAtlasiP36041.

    Expressioni

    Gene expression databases

    GenevestigatoriP36041.

    Interactioni

    Subunit structurei

    Interacts with SMY2, SYH1 and eIF4E.2 Publications

    Protein-protein interaction databases

    BioGridi33919. 67 interactions.
    DIPiDIP-1778N.
    IntActiP36041. 11 interactions.
    MINTiMINT-394822.
    STRINGi4932.YKL204W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36041.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG12793.
    KOiK15908.
    OMAiNDERRMS.
    OrthoDBiEOG7J18CB.

    Sequencei

    Sequence statusi: Complete.

    P36041-1 [UniParc]FASTAAdd to Basket

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    MELNDPSIIS SSQFSGELSD SDTAAATHKS QQAISNLFQK LAKKGREEKP    50
    IGSVESSTDS SNISVATSGN NKESNKKKNK KTAMLNFSSL TDPITNYKPM 100
    DLQYKTYAYS MNELYHLKPS LASASYEEDP LISELVRSLP KRKFWRLRMG 150
    PPDQKHANNH HFNGNNGGGS WKAGYKNGKN DERRMSRTKN MQGGKRRSQQ 200
    DDEEKKIDQE MLEMDKNLQL GGDVGHSIAD FEDWKAKMKE LELKKLSKSK 250
    GISNSTAIAP RESASHETPT DLRPVIPRGP SSITDFLNLK RQDKKEESSQ 300
    QTPGIPVGQP SLSKTSIEQV NELETNSDLG KSSSSRFSSF FNKSATSLPS 350
    LDNNNQVPSS NVSVVNNDGN STPHQSGSRL MSFFKESRSS TPNAESQLLS 400
    ASDKDNGKMQ TLPQFQQQPQ QMQPMAFTQH PPNNNAFFNG LLNKGKSETS 450
    TPPPPPPGLI AHQGPQFPVM GVPPNFPQRM MPPPPGLVQF QKDSKDVNKK 500
    EDRQLRQNKN PNGTRNSKGK QEETATPDLP QQQYMPPPPP PGFFPMHPNF 550
    PNGPMPPLPQ GFPIPPNGML PVTGQQPQPP YPNMMLQGNF PPNFQQGFGS 600
    NSPMPIPSII NANGKNVTNQ LPPGLNSKKN IK 632
    Length:632
    Mass (Da):69,762
    Last modified:June 1, 1994 - v1
    Checksum:i775F73F57BB0EF2B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28204 Genomic DNA. Translation: CAA82049.1.
    BK006944 Genomic DNA. Translation: DAA08965.1.
    PIRiS38042.
    RefSeqiNP_012718.1. NM_001179769.1.

    Genome annotation databases

    EnsemblFungiiYKL204W; YKL204W; YKL204W.
    GeneIDi853631.
    KEGGisce:YKL204W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28204 Genomic DNA. Translation: CAA82049.1 .
    BK006944 Genomic DNA. Translation: DAA08965.1 .
    PIRi S38042.
    RefSeqi NP_012718.1. NM_001179769.1.

    3D structure databases

    ProteinModelPortali P36041.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33919. 67 interactions.
    DIPi DIP-1778N.
    IntActi P36041. 11 interactions.
    MINTi MINT-394822.
    STRINGi 4932.YKL204W.

    Proteomic databases

    MaxQBi P36041.
    PaxDbi P36041.
    PeptideAtlasi P36041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL204W ; YKL204W ; YKL204W .
    GeneIDi 853631.
    KEGGi sce:YKL204W.

    Organism-specific databases

    CYGDi YKL204w.
    SGDi S000001687. EAP1.

    Phylogenomic databases

    eggNOGi NOG12793.
    KOi K15908.
    OMAi NDERRMS.
    OrthoDBi EOG7J18CB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31963-MONOMER.

    Miscellaneous databases

    NextBioi 974508.

    Gene expression databases

    Genevestigatori P36041.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability."
      Chial H.J., Stemm-Wolf A.J., McBratney S., Winey M.
      Curr. Biol. 10:1519-1522(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae."
      Cosentino G.P., Schmelzle T., Haghighat A., Helliwell S.B., Hall M.N., Sonenberg N.
      Mol. Cell. Biol. 20:4604-4613(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EIF4E, MUTAGENESIS OF TYR-109.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation."
      Matsuo R., Kubota H., Obata T., Kito K., Ota K., Kitazono T., Ibayashi S., Sasaki T., Iida M., Ito T.
      FEBS Lett. 579:2433-2438(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TYR-109 AND LEU-114.
    8. "GYF domain proteomics reveals interaction sites in known and novel target proteins."
      Kofler M., Motzny K., Freund C.
      Mol. Cell. Proteomics 4:1797-1811(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SMY2 AND SYH1.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30; SER-281; SER-282; SER-327; SER-344 AND SER-387, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282 AND SER-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEAP1_YEAST
    AccessioniPrimary (citable) accession number: P36041
    Secondary accession number(s): D6VWZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3240 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3