ADD66

Functionⁱ

Involved in 20S proteasome assembly. Required for maximal proteasome activity. Affects the chymotrypsin-like activity of the proteasome. Can be degraded by the proteasome. Involved in the endoplasmic reticulum-associated degradation (ERAD).3 Publications

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication

Deletion induces the unfolded protein response (UPR).

GO - Biological processⁱ

proteasome assembly
Source: SGD
Inferred from mutant phenotypeⁱ
- 17634286

Complete GO annotation...

Keywordsⁱ

Molecular function	Chaperone

Molecular function

Chaperone

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31965-MONOMER.

BioCycⁱ

YEAST:G3O-31965-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Proteasome assembly chaperone 2 Alternative name(s): Alpha-1-proteinase inhibitor-degradation deficient protein 66 Proteasome biogenesis-associated protein 2
Gene namesⁱ	Name:ADD66 Synonyms:PBA2, POC2 Ordered Locus Names:YKL206C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XI

Organism-specific databases

EuPathDBⁱ	FungiDB:YKL206C.
Saccharomyces Genome Database More...SGDⁱ	S000001689. ADD66.

Subcellular locationⁱ

Cytoplasm
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly."
  Scott C.M., Kruse K.B., Schmidt B.Z., Perlmutter D.H., McCracken A.A., Brodsky J.L.
  Mol. Biol. Cell 18:3776-3787(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.

GO - Cellular componentⁱ

cytosol
Source: SGD
Inferred from direct assayⁱ
- 17634286
nucleus Source: GO_Central
proteasome complex Source: GO_Central

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000203132	1 – 267	Proteasome assembly chaperone 2Add BLAST		267

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P36040.
PRIDEⁱ	P36040.

Interactionⁱ

Subunit structureⁱ

Component of the 20S proteasome chaperone. Forms a heterodimer with PBA1 that binds to proteasome precursors.2 Publications

Protein-protein interaction databases

BioGridⁱ	33917. 155 interactors.
Database of interacting proteins More...DIPⁱ	DIP-6515N.
Molecular INTeraction database More...MINTⁱ	MINT-700530.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	3 – 7	Combined sources	5
Helixⁱ	11 – 13	Combined sources	3
Helixⁱ	14 – 25	Combined sources	12
Beta strandⁱ	28 – 35	Combined sources	8
Beta strandⁱ	51 – 54	Combined sources	4
Beta strandⁱ	67 – 75	Combined sources	9
Turnⁱ	76 – 79	Combined sources	4
Beta strandⁱ	80 – 85	Combined sources	6
Helixⁱ	92 – 94	Combined sources	3
Helixⁱ	95 – 108	Combined sources	14
Helixⁱ	109 – 111	Combined sources	3
Beta strandⁱ	113 – 118	Combined sources	6
Helixⁱ	183 – 188	Combined sources	6
Helixⁱ	220 – 228	Combined sources	9
Helixⁱ	247 – 251	Combined sources	5
Helixⁱ	258 – 266	Combined sources	9

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4G4S	X-ray	2.49	P	1-267	[»]
ProteinModelPortalⁱ	P36040.
SMRⁱ	P36040.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the PSMG2 family.Curated

Phylogenomic databases

HOGENOMⁱ	HOG000066091.
InParanoidⁱ	P36040.
KEGG Orthology (KO) More...KOⁱ	K11880.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4LOR.

Family and domain databases

InterProⁱ	View protein in InterPro IPR019151. Proteasome_assmbl_chaperone_2. IPR016562. Proteasome_assmbl_chp_2_euk.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09754. PAC2. 1 hit.
PIRSFⁱ	PIRSF010044. UCP010044. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P36040-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSCLVLPLVS VGNIPQLSID WLLNSQANEW EYLEALDSKY LVEFVGPLDR 
        60         70         80         90        100
PEDGSDSLYK DADMKYSSAL EVFYNKKRGL FAIQQRTPLV SVNYLNNFIV 
       110        120        130        140        150
EIILPFLSKY NISEICIWDS LYAMEDENGV IVRPQEVYSL GEFYFDDEAE 
       160        170        180        190        200
LLSNLHLNDQ ESMVNNWLHF TPTSFQDKIS VDQPIFKILF QILNASQRPK 
       210        220        230        240        250
ALRSIKYCSC LANEGDNSLD SQQFLQWIIS QKVIKNAPPI VKFVRPISWQ 
       260 
GAYGMADARD KFVDLYN

Length:267

Mass (Da):30,739

Last modified:June 1, 1994 - v1

Checksum:ⁱD635C3EBC2E37C11

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z28206 Genomic DNA. Translation: CAA82051.1. AY558394 Genomic DNA. Translation: AAS56720.1. BK006944 Genomic DNA. Translation: DAA08963.1.
PIRⁱ	S38044.
NCBI Reference Sequences More...RefSeqⁱ	NP_012716.1. NM_001179771.1.

Genome annotation databases

EnsemblFungiⁱ	YKL206C; YKL206C; YKL206C.
GeneIDⁱ	853629.
KEGGⁱ	sce:YKL206C.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z28206 Genomic DNA. Translation: CAA82051.1. AY558394 Genomic DNA. Translation: AAS56720.1. BK006944 Genomic DNA. Translation: DAA08963.1.
PIRⁱ	S38044.
NCBI Reference Sequences More...RefSeqⁱ	NP_012716.1. NM_001179771.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4G4S	X-ray	2.49	P	1-267	[»]
ProteinModelPortalⁱ	P36040.
SMRⁱ	P36040.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	33917. 155 interactors.
Database of interacting proteins More...DIPⁱ	DIP-6515N.
Molecular INTeraction database More...MINTⁱ	MINT-700530.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P36040.
PRIDEⁱ	P36040.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YKL206C; YKL206C; YKL206C.
GeneIDⁱ	853629.
KEGGⁱ	sce:YKL206C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YKL206C.
Saccharomyces Genome Database More...SGDⁱ	S000001689. ADD66.

Phylogenomic databases

HOGENOMⁱ	HOG000066091.
InParanoidⁱ	P36040.
KEGG Orthology (KO) More...KOⁱ	K11880.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4LOR.

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31965-MONOMER.

BioCycⁱ

YEAST:G3O-31965-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P36040.

PROⁱ

PR:P36040.

Family and domain databases

InterProⁱ	View protein in InterPro IPR019151. Proteasome_assmbl_chaperone_2. IPR016562. Proteasome_assmbl_chp_2_euk.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09754. PAC2. 1 hit.
PIRSFⁱ	PIRSF010044. UCP010044. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	POC2_YEAST
Accessionⁱ	P36040Primary (citable) accession number: P36040 Secondary accession number(s): D6VWZ7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
	Last sequence update:	June 1, 1994
	Last modified:	March 15, 2017
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P36040 (POC2_YEAST)

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Proteasome assembly chaperone 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ