P36040 (POC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome assembly chaperone 2

Alternative name(s):
Alpha-1-proteinase inhibitor-degradation deficient protein 66
Proteasome biogenesis-associated protein 2

Gene names

Name:	ADD66
Synonyms:	PBA2, POC2
Ordered Locus Names:	YKL206C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in 20S proteasome assembly. Required for maximal proteasome activity. Affects the chymotrypsin-like activity of the proteasome. Can be degraded by the proteasome. Involved in the endoplasmic reticulum-associated degradation (ERAD). Ref.5 Ref.7 Ref.8
Subunit structure	Component of the 20S proteasome chaperone. Forms a heterodimer with PBA1 that binds to proteasome precursors. Ref.4 Ref.8
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 2940 molecules/cell in log phase SD medium. Ref.6 Deletion induces the unfolded protein response (UPR).
Sequence similarities	Belongs to the PSMG2 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Inferred from mutant phenotype Ref.5. Source: SGD proteasome assembly Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cytosol Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARL3	Q02804	1	EBI-27001,EBI-30136

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Proteasome assembly chaperone 2		PRO_0000203132

Sequences

	Sequence		Length	Mass (Da)
	P36040 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D635C3EBC2E37C11	FASTA	267	30,739
	10 20 30 40 50 60 MSCLVLPLVS VGNIPQLSID WLLNSQANEW EYLEALDSKY LVEFVGPLDR PEDGSDSLYK 70 80 90 100 110 120 DADMKYSSAL EVFYNKKRGL FAIQQRTPLV SVNYLNNFIV EIILPFLSKY NISEICIWDS 130 140 150 160 170 180 LYAMEDENGV IVRPQEVYSL GEFYFDDEAE LLSNLHLNDQ ESMVNNWLHF TPTSFQDKIS 190 200 210 220 230 240 VDQPIFKILF QILNASQRPK ALRSIKYCSC LANEGDNSLD SQQFLQWIIS QKVIKNAPPI 250 260 VKFVRPISWQ GAYGMADARD KFVDLYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Beta-subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint." Li X., Kusmierczyk A.R., Wong P., Emili A., Hochstrasser M. EMBO J. 26:2339-2349(2007) [PubMed: 17431397] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-76; 79-86; 179-187 AND 243-259, GENE NAME, INTERACTION WITH PBA1, SUBUNIT, MASS SPECTROMETRY.
[5]	"Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation." Palmer E.A., Kruse K.B., Fewell S.W., Buchanan S.M., Brodsky J.L., McCracken A.A. J. Cell Sci. 116:2361-2373(2003) [PubMed: 12711700] [Abstract] Cited for: GENE NAME, FUNCTION, DELETION MUTANT.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly." Scott C.M., Kruse K.B., Schmidt B.Z., Perlmutter D.H., McCracken A.A., Brodsky J.L. Mol. Biol. Cell 18:3776-3787(2007) [PubMed: 17634286] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals." Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R., Marsolier-Kergoat M.-C., Peyroche A. Mol. Cell 27:660-674(2007) [PubMed: 17707236] [Abstract] Cited for: GENE NAME, FUNCTION, INTERACTION WITH PBA1, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28206 Genomic DNA. Translation: CAA82051.1. AY558394 Genomic DNA. Translation: AAS56720.1. BK006944 Genomic DNA. Translation: DAA08963.1.
PIR	S38044.
RefSeq	NP_012716.1. NM_001179771.1.
3D structure databases
ProteinModelPortal	P36040.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6515N.
IntAct	P36040. 2 interactions.
MINT	MINT-700530.
STRING	P36040.
Proteomic databases
PeptideAtlas	P36040.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL206C; YKL206C; YKL206C.
GeneID	853629.
KEGG	sce:YKL206C.
NMPDR	fig|4932.3.peg.3694.
Organism-specific databases
CYGD	YKL206c.
SGD	S000001689. ADD66.
Phylogenomic databases
eggNOG	fuNOG10053.
HOGENOM	HBG202343.
OrthoDB	EOG4TQQKH.
Gene expression databases
ArrayExpress	P36040.
Genevestigator	P36040.
GermOnline	YKL206C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019151. Proteasome_assmbl_chaperone_2. IPR016562. Proteasome_assmbl_chp_2_euk. [Graphical view]
KO	K11880.
Pfam	PF09754. PAC2. 1 hit. [Graphical view]
PIRSF	PIRSF010044. UCP010044. 1 hit.
ProtoNet	Search...
Other
NextBio	974502.

Entry information

Entry name

POC2_YEAST

Accession

Primary (citable) accession number: P36040
Secondary accession number(s): D6VWZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program