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Protein

Proteasome assembly chaperone 2

Gene

ADD66

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in 20S proteasome assembly. Required for maximal proteasome activity. Affects the chymotrypsin-like activity of the proteasome. Can be degraded by the proteasome. Involved in the endoplasmic reticulum-associated degradation (ERAD).3 Publications

GO - Biological processi

  • proteasome assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-31965-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome assembly chaperone 2
Alternative name(s):
Alpha-1-proteinase inhibitor-degradation deficient protein 66
Proteasome biogenesis-associated protein 2
Gene namesi
Name:ADD66
Synonyms:PBA2, POC2
Ordered Locus Names:YKL206C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL206C.
SGDiS000001689. ADD66.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: GO_Central
  • proteasome complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002031321 – 267Proteasome assembly chaperone 2Add BLAST267

Proteomic databases

MaxQBiP36040.
PRIDEiP36040.

Interactioni

Subunit structurei

Component of the 20S proteasome chaperone. Forms a heterodimer with PBA1 that binds to proteasome precursors.2 Publications

Protein-protein interaction databases

BioGridi33917. 35 interactors.
DIPiDIP-6515N.
MINTiMINT-700530.

Structurei

Secondary structure

1267
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi11 – 13Combined sources3
Helixi14 – 25Combined sources12
Beta strandi28 – 35Combined sources8
Beta strandi51 – 54Combined sources4
Beta strandi67 – 75Combined sources9
Turni76 – 79Combined sources4
Beta strandi80 – 85Combined sources6
Helixi92 – 94Combined sources3
Helixi95 – 108Combined sources14
Helixi109 – 111Combined sources3
Beta strandi113 – 118Combined sources6
Helixi183 – 188Combined sources6
Helixi220 – 228Combined sources9
Helixi247 – 251Combined sources5
Helixi258 – 266Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G4SX-ray2.49P1-267[»]
ProteinModelPortaliP36040.
SMRiP36040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PSMG2 family.Curated

Phylogenomic databases

HOGENOMiHOG000066091.
InParanoidiP36040.
KOiK11880.
OrthoDBiEOG092C4LOR.

Family and domain databases

InterProiIPR019151. Proteasome_assmbl_chaperone_2.
IPR016562. Proteasome_assmbl_chp_2_euk.
[Graphical view]
PfamiPF09754. PAC2. 1 hit.
[Graphical view]
PIRSFiPIRSF010044. UCP010044. 1 hit.

Sequencei

Sequence statusi: Complete.

P36040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCLVLPLVS VGNIPQLSID WLLNSQANEW EYLEALDSKY LVEFVGPLDR
60 70 80 90 100
PEDGSDSLYK DADMKYSSAL EVFYNKKRGL FAIQQRTPLV SVNYLNNFIV
110 120 130 140 150
EIILPFLSKY NISEICIWDS LYAMEDENGV IVRPQEVYSL GEFYFDDEAE
160 170 180 190 200
LLSNLHLNDQ ESMVNNWLHF TPTSFQDKIS VDQPIFKILF QILNASQRPK
210 220 230 240 250
ALRSIKYCSC LANEGDNSLD SQQFLQWIIS QKVIKNAPPI VKFVRPISWQ
260
GAYGMADARD KFVDLYN
Length:267
Mass (Da):30,739
Last modified:June 1, 1994 - v1
Checksum:iD635C3EBC2E37C11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28206 Genomic DNA. Translation: CAA82051.1.
AY558394 Genomic DNA. Translation: AAS56720.1.
BK006944 Genomic DNA. Translation: DAA08963.1.
PIRiS38044.
RefSeqiNP_012716.1. NM_001179771.1.

Genome annotation databases

EnsemblFungiiYKL206C; YKL206C; YKL206C.
GeneIDi853629.
KEGGisce:YKL206C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28206 Genomic DNA. Translation: CAA82051.1.
AY558394 Genomic DNA. Translation: AAS56720.1.
BK006944 Genomic DNA. Translation: DAA08963.1.
PIRiS38044.
RefSeqiNP_012716.1. NM_001179771.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G4SX-ray2.49P1-267[»]
ProteinModelPortaliP36040.
SMRiP36040.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33917. 35 interactors.
DIPiDIP-6515N.
MINTiMINT-700530.

Proteomic databases

MaxQBiP36040.
PRIDEiP36040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL206C; YKL206C; YKL206C.
GeneIDi853629.
KEGGisce:YKL206C.

Organism-specific databases

EuPathDBiFungiDB:YKL206C.
SGDiS000001689. ADD66.

Phylogenomic databases

HOGENOMiHOG000066091.
InParanoidiP36040.
KOiK11880.
OrthoDBiEOG092C4LOR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31965-MONOMER.

Miscellaneous databases

PROiP36040.

Family and domain databases

InterProiIPR019151. Proteasome_assmbl_chaperone_2.
IPR016562. Proteasome_assmbl_chp_2_euk.
[Graphical view]
PfamiPF09754. PAC2. 1 hit.
[Graphical view]
PIRSFiPIRSF010044. UCP010044. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPOC2_YEAST
AccessioniPrimary (citable) accession number: P36040
Secondary accession number(s): D6VWZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication
Deletion induces the unfolded protein response (UPR).

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.