Reviewed, UniProtKB/Swiss-Prot P36040 (POC2_YEAST)

Names and origin

Protein names

Recommended name:
    Proteasome chaperone 2
Alternative name(s):
    Proteasome biogenesis-associated protein 2
    Alpha-1-proteinase inhibitor-degradation deficient protein 66

Gene names

Name:	ADD66
Synonyms:	PBA2, POC2
Ordered Locus Names:	YKL206C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in 20S proteasome assembly. Required for maximal proteasome activity. Affects the chymotrypsin-like activity of the proteasome. Can be degraded by the proteasome. Involved in the endoplasmic reticulum-associated degradation (ERAD). Ref.4 Ref.6 Ref.7
Subunit structure	Component of the 20S proteasome chaperone. Forms a heterodimer with PBA1 that binds to proteasome precursors. Ref.7 Ref.3
Subcellular location	Cytoplasm.
Miscellaneous	Present with 2940 molecules/cell in log phase SD medium. Ref.5 Deletion induces the unfolded protein response (UPR).
Sequence similarities	Belongs to the PSMG2 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Ref.4 Inferred from mutant phenotype. Source: SGD proteasome assembly Ref.3 Ref.6 Inferred from mutant phenotype. Source: SGD
Cellular component	cytosol Ref.6 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARL3	Q02804	1	EBI-27001,EBI-30136

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Proteasome chaperone 2		PRO_0000203132

Sequences

	Sequence		Length	Mass (Da)
	P36040-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D635C3EBC2E37C11	FASTA	267	30,739
	10 20 30 40 50 60 MSCLVLPLVS VGNIPQLSID WLLNSQANEW EYLEALDSKY LVEFVGPLDR PEDGSDSLYK 70 80 90 100 110 120 DADMKYSSAL EVFYNKKRGL FAIQQRTPLV SVNYLNNFIV EIILPFLSKY NISEICIWDS 130 140 150 160 170 180 LYAMEDENGV IVRPQEVYSL GEFYFDDEAE LLSNLHLNDQ ESMVNNWLHF TPTSFQDKIS 190 200 210 220 230 240 VDQPIFKILF QILNASQRPK ALRSIKYCSC LANEGDNSLD SQQFLQWIIS QKVIKNAPPI 250 260 VKFVRPISWQ GAYGMADARD KFVDLYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Beta-subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint." Li X., Kusmierczyk A.R., Wong P., Emili A., Hochstrasser M. EMBO J. 26:2339-2349(2007) [PubMed: 17431397] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-76; 79-86; 179-187 AND 243-259, GENE NAME, INTERACTION WITH PBA1, SUBUNIT, MASS SPECTROMETRY.
[4]	"Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation." Palmer E.A., Kruse K.B., Fewell S.W., Buchanan S.M., Brodsky J.L., McCracken A.A. J. Cell Sci. 116:2361-2373(2003) [PubMed: 12711700] [Abstract] Cited for: GENE NAME, FUNCTION, DELETION MUTANT.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly." Scott C.M., Kruse K.B., Schmidt B.Z., Perlmutter D.H., McCracken A.A., Brodsky J.L. Mol. Biol. Cell 18:3776-3787(2007) [PubMed: 17634286] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals." Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R., Marsolier-Kergoat M.-C., Peyroche A. Mol. Cell 27:660-674(2007) [PubMed: 17707236] [Abstract] Cited for: GENE NAME, FUNCTION, INTERACTION WITH PBA1, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28206 Genomic DNA. Translation: CAA82051.1. AY558394 Genomic DNA. Translation: AAS56720.1.
PIR	S38044.
RefSeq	NP_012716.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6515N.
IntAct	P36040. 2 interactions.
STRING	P36040.
Proteomic databases
PeptideAtlas	P36040.
PRIDE	P36040.
Genome annotation databases
Ensembl	YKL206C; YKL206C; YKL206C; Saccharomyces cerevisiae. [Genome view]
GeneID	853629.
KEGG	sce:YKL206C.
NMPDR	fig|4932.3.peg.3694.
Organism-specific databases
CYGD	YKL206c.
SGD	S000001689. ADD66.
Phylogenomic databases
HOGENOM	P36040.
OrthoDB	EOG9PK3R5
Gene expression databases
ArrayExpress	P36040.
Genevestigator	P36040.
GermOnline	YKL206C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019151. 20S_Psome_assmbly_chp2. IPR016562. 20S_Psome_chp2_sbgrp. [Graphical view]
Pfam	PF09754. HCCA3. 1 hit. [Graphical view]
PIRSF	PIRSF010044. UCP010044. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	974502.

Entry information

Entry name

POC2_YEAST

Accession

Primary (citable) accession number: P36040

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)