Reviewed, UniProtKB/Swiss-Prot P36039 (EMC3_YEAST)

Names and origin

Protein names

Recommended name:
    ER membrane protein complex subunit 3
Alternative name(s):
    Altered inheritance rate of mitochondria protein 27

Gene names

Name:	AIM27
Synonyms:	EMC3
Ordered Locus Names:	YKL207W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).
Subunit structure	Component of the ER membrane protein complex (EMC), which is composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the UPF0347 (TMEM111) family.
Sequence caution	The sequence CAA82052.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA82052.1 differs from that shown. Reason: Frameshift at position 212.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG25	P53045	1	EBI-27005,EBI-6506
ERV25	P54837	1	EBI-27005,EBI-6642
ERV29	P53337	1	EBI-27005,EBI-23662
GAP1	P19145	1	EBI-27005,EBI-7314
KRE27	P40540	1	EBI-27005,EBI-24977
ORM2	Q06144	1	EBI-27005,EBI-34916
SWP1	Q02795	1	EBI-27005,EBI-12666

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	ER membrane protein complex subunit 3		PRO_0000211411
Regions
	Transmembrane	10 – 30	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	176 – 196	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P36039-1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 499475DA44B93B3E	FASTA	253	28,352
	10 20 30 40 50 60 MLLDDQLKYW VLLPISIVMV LTGVLKQYIM TLITGSSANE AQPRVKLTEW QYLQWAQLLI 70 80 90 100 110 120 GNGGNLSSDA FAAKKEFLVK DLTEERHLAK AKQQDGSQAG EVPNPFNDPS MSNAMMNMAK 130 140 150 160 170 180 GNMASFIPQT IIMWWVNHFF AGFILMQLPF PLTAKFKEML QTGIICQDLD VRWVSSISWY 190 200 210 220 230 240 FISVLGLNPV YNLIGLNDQD MGIQAGIGGP QGPQGPPQSQ VDKAMHAMAN DLTIIQHETC 250 LDNVEQRVLK QYM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:RESEARCH45.1-RESEARCH45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-253. Strain: ATCC 204511 / S288c / AB972.
[3]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[4]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed: 12775844] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum." Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M. Science 323:1693-1697(2009) [PubMed: 19325107] [Abstract] Cited for: IDENTIFICATION IN EMC COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28207 Genomic DNA. Translation: CAA82052.1. Sequence problems. AY260882 Genomic DNA. Translation: AAP21750.1.
PIR	S38045.
RefSeq	NP_012715.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P36039. 9 interactions.
Proteomic databases
PeptideAtlas	P36039.
Genome annotation databases
Ensembl	YKL207W; YKL207W; YKL207W; Saccharomyces cerevisiae. [Genome view]
GeneID	853628.
GenomeReviews	Gene locus YKL207W in contig Y13137_GR.
KEGG	sce:YKL207W.
NMPDR	fig|4932.3.peg.3693.
Organism-specific databases
CYGD	YKL207w.
SGD	S000001690. AIM27.
Phylogenomic databases
HOGENOM	P36039.
OMA	HAMANDL.
Gene expression databases
ArrayExpress	P36039.
Genevestigator	P36039.
GermOnline	YKL207W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008568. DUF850_TM_euk. [Graphical view]
PANTHER	PTHR13116. DUF850_TM_euk. 1 hit.
Pfam	PF05863. DUF850. 1 hit. [Graphical view]
PIRSF	PIRSF010045. DUF850_TM_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EMC3_YEAST

Accession

Primary (citable) accession number: P36039
Secondary accession number(s): Q86ZT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 16, 2009
Last modified:	November 3, 2009
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)