P36039 (EMC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ER membrane protein complex subunit 3

Alternative name(s):
Altered inheritance rate of mitochondria protein 27

Gene names

Name:	AIM27
Synonyms:	EMC3
Ordered Locus Names:	YKL207W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).
Subunit structure	Component of the ER membrane protein complex (EMC), which is composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the UPF0347 (TMEM111) family.
Sequence caution	The sequence CAA82052.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA82052.1 differs from that shown. Reason: Frameshift at position 212.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding in endoplasmic reticulum Inferred from genetic interaction Ref.6. Source: SGD
Cellular component	ER membrane protein complex Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG25	P53045	1	EBI-27005,EBI-6506
ERV25	P54837	1	EBI-27005,EBI-6642
ERV29	P53337	1	EBI-27005,EBI-23662
GAP1	P19145	1	EBI-27005,EBI-7314
KRE27	P40540	1	EBI-27005,EBI-24977
ORM2	Q06144	1	EBI-27005,EBI-34916
SWP1	Q02795	1	EBI-27005,EBI-12666

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	ER membrane protein complex subunit 3		PRO_0000211411
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P36039 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 499475DA44B93B3E	FASTA	253	28,352
	10 20 30 40 50 60 MLLDDQLKYW VLLPISIVMV LTGVLKQYIM TLITGSSANE AQPRVKLTEW QYLQWAQLLI 70 80 90 100 110 120 GNGGNLSSDA FAAKKEFLVK DLTEERHLAK AKQQDGSQAG EVPNPFNDPS MSNAMMNMAK 130 140 150 160 170 180 GNMASFIPQT IIMWWVNHFF AGFILMQLPF PLTAKFKEML QTGIICQDLD VRWVSSISWY 190 200 210 220 230 240 FISVLGLNPV YNLIGLNDQD MGIQAGIGGP QGPQGPPQSQ VDKAMHAMAN DLTIIQHETC 250 LDNVEQRVLK QYM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-253. Strain: ATCC 204511 / S288c / AB972.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed: 12775844] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[6]	"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum." Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M. Science 323:1693-1697(2009) [PubMed: 19325107] [Abstract] Cited for: IDENTIFICATION IN EMC COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28207 Genomic DNA. Translation: CAA82052.1. Sequence problems. AY260882 Genomic DNA. Translation: AAP21750.1. BK006944 Genomic DNA. Translation: DAA08962.1.
PIR	S38045.
RefSeq	NP_012715.3. NM_001179772.1.
3D structure databases
ProteinModelPortal	P36039.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36039. 8 interactions.
STRING	P36039.
Proteomic databases
PeptideAtlas	P36039.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL207W; YKL207W; YKL207W.
GeneID	853628.
KEGG	sce:YKL207W.
NMPDR	fig|4932.3.peg.3693.
Organism-specific databases
SGD	S000001690. AIM27.
Phylogenomic databases
eggNOG	fuNOG08483.
GeneTree	EFGT00050000005357.
HOGENOM	HBG626693.
OMA	MAKGNMA.
OrthoDB	EOG454D7K.
Gene expression databases
ArrayExpress	P36039.
Genevestigator	P36039.
GermOnline	YKL207W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002809. DUF106_TM. IPR008568. UCP010045_TM_euk. [Graphical view]
PANTHER	PTHR13116. DUF850_TM_euk. 1 hit.
Pfam	PF01956. DUF106. 1 hit. [Graphical view]
PIRSF	PIRSF010045. DUF850_TM_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EMC3_YEAST

Accession

Primary (citable) accession number: P36039
Secondary accession number(s): D6VWZ6, Q86ZT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 16, 2009
Last modified:	December 14, 2011
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program