Reviewed, UniProtKB/Swiss-Prot P36037 (DOA1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein DOA1

Gene names

Name:	DOA1
Synonyms:	UFD3, ZZZ4
Ordered Locus Names:	YKL213C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in ubiquitin conjugation pathway. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May act by preventing the interaction between CDC48 and the E4 enzyme UFD2, leading to prevent multiubiquitination of substrates and subsequent degradation. Ref.4 Ref.5
Subunit structure	Interacts with CDC48 and OTU1. Ref.8
Subcellular location	Nucleus. Cytoplasm Ref.6.
Miscellaneous	Present with 6800 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the WD repeat PLAP family. Contains 1 PFU domain. Contains 1 PUL domain. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	double-strand break repair via nonhomologous end joining Inferred from mutant phenotype. Source: SGD ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.6 Inferred from direct assay. Source: SGD nucleus Ref.6 Inferred from direct assay. Source: SGD
Molecular function	ubiquitin binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Protein DOA1		PRO_0000050958
Regions
	Repeat	11 – 40	30	WD 1
	Repeat	53 – 82	30	WD 2
	Repeat	97 – 125	29	WD 3
	Repeat	135 – 166	32	WD 4
	Repeat	177 – 206	30	WD 5
	Repeat	218 – 247	30	WD 6
	Repeat	259 – 288	30	WD 7
	Domain	352 – 449	98	PFU
	Domain	465 – 715	251	PUL
Amino acid modifications
	Modified residue	332	1	Phosphoserine Ref.9 Ref.10
	Modified residue	454	1	Phosphoserine Ref.10
	Modified residue	457	1	Phosphoserine Ref.9 Ref.10
	Modified residue	459	1	Phosphoserine Ref.10
Experimental info
	Sequence conflict	180	1	D → DI AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36037-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 593B808169283B5F	FASTA	715	79,506
	10 20 30 40 50 60 MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV YTGQGFLNSV 70 80 90 100 110 120 CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG NVCSLSFQDG VVISGSWDKT 130 140 150 160 170 180 AKVWKEGSLV YNLQAHNASV WDAKVVSFSE NKFLTASADK TIKLWQNDKV IKTFSGIHND 190 200 210 220 230 240 VVRHLAVVDD GHFISCSNDG LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED 250 260 270 280 290 300 RTVRIWSKEN GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI 310 320 330 340 350 360 NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI EAHQFSNSSW 370 380 390 400 410 420 KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL KLPINVSDNP YTAADNFLAR 430 440 450 460 470 480 YELPMSYRDQ VVQFILKNTN GISLDQPNDN ASSSAVSPSK TSVMKVLPVK QYLIMENYNP 490 500 510 520 530 540 DTIFNGIVKI NSNEKTFDDE ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV 550 560 570 580 590 600 RLIVKKLPYS SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF 610 620 630 640 650 660 ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI NTKYGPLEEY 670 680 690 700 710 QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS YGNVPRFKDI FDDLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hochstrasser M., Gang G. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed: 7941750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Identification of a novel 29-linked polyubiquitin binding protein, Ufd3, using polyubiquitin chain analogues." Russell N.S., Wilkinson K.D. Biochemistry 43:4844-4854(2004) [PubMed: 15096053] [Abstract] Cited for: PROTEIN SEQUENCE OF 126-144; 173-183; 215-227; 316-335; 345-361; 384-420; 534-541; 598-613; 654-685 AND 700-706, FUNCTION.
[5]	"Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae." Ghislain M., Dohmen R.J., Levy F., Varshavsky A. EMBO J. 15:4884-4899(1996) [PubMed: 8890162] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone." Rumpf S., Jentsch S. Mol. Cell 21:261-269(2006) [PubMed: 16427015] [Abstract] Cited for: INTERACTION WITH OTU1 AND CDC48.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-457, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332; SER-454; SER-457 AND SER-459, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U39947 Genomic DNA. Translation: AAA82258.1. X75951 Genomic DNA. Translation: CAA53560.1. Z28213 Genomic DNA. Translation: CAA82058.1.
PIR	S38051.
RefSeq	NP_012709.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6274N.
IntAct	P36037. 9 interactions.
STRING	P36037.
Proteomic databases
PeptideAtlas	P36037.
Genome annotation databases
Ensembl	YKL213C; YKL213C; YKL213C; Saccharomyces cerevisiae. [Genome view]
GeneID	853667.
KEGG	sce:YKL213C.
NMPDR	fig|4932.3.peg.3687.
Organism-specific databases
CYGD	YKL213c.
SGD	S000001696. DOA1.
Phylogenomic databases
HOGENOM	HBG395734.
OMA	HESFVYC.
OrthoDB	EOG90S1ZF.
Gene expression databases
ArrayExpress	P36037.
Genevestigator	P36037.
GermOnline	YKL213C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep_reg. IPR015155. PLAA_fam_Ub_bd_PFU. IPR013535. PUL. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF09070. PFU. 1 hit. PF08324. PUL. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 6 hits. [Graphical view]
PROSITE	PS51394. PFU. 1 hit. PS51396. PUL. 1 hit. PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974605.

Entry information

Entry name

DOA1_YEAST

Accession

Primary (citable) accession number: P36037

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 15, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)