Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein DOA1

Gene

DOA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May act by preventing the interaction between CDC48 and the E4 enzyme UFD2, leading to prevent multiubiquitination of substrates and subsequent degradation. Essential for maintaining cellular ubiquitin levels.2 Publications

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • double-strand break repair via nonhomologous end joining Source: SGD
  • mitochondria-associated ubiquitin-dependent protein catabolic process Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • ribophagy Source: SGD
  • ubiquitin recycling Source: SGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31971-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DOA1
Gene namesi
Name:DOA1
Synonyms:UFD3, ZZZ4
Ordered Locus Names:YKL213C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL213C
SGDiS000001696 DOA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi541R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. 1 Publication1
Mutagenesisi669R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509581 – 715Protein DOA1Add BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei332PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36037
PaxDbiP36037
PRIDEiP36037

PTM databases

iPTMnetiP36037

Interactioni

Subunit structurei

Interacts with CDC48, OTU1 and ubiquitin.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33952, 679 interactors
DIPiDIP-6274N
IntActiP36037, 10 interactors
MINTiP36037
STRINGi4932.YKL213C

Structurei

Secondary structure

1715
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Beta strandi16 – 23Combined sources8
Beta strandi26 – 31Combined sources6
Beta strandi34 – 52Combined sources19
Beta strandi57 – 63Combined sources7
Turni64 – 67Combined sources4
Beta strandi68 – 73Combined sources6
Beta strandi78 – 82Combined sources5
Beta strandi102 – 108Combined sources7
Beta strandi111 – 116Combined sources6
Beta strandi119 – 125Combined sources7
Beta strandi128 – 134Combined sources7
Beta strandi140 – 147Combined sources8
Turni148 – 151Combined sources4
Beta strandi152 – 157Combined sources6
Beta strandi162 – 166Combined sources5
Beta strandi169 – 174Combined sources6
Beta strandi182 – 189Combined sources8
Beta strandi192 – 197Combined sources6
Beta strandi200 – 206Combined sources7
Turni207 – 209Combined sources3
Beta strandi212 – 217Combined sources6
Beta strandi223 – 228Combined sources6
Beta strandi234 – 238Combined sources5
Beta strandi241 – 246Combined sources6
Turni248 – 250Combined sources3
Beta strandi253 – 258Combined sources6
Beta strandi260 – 262Combined sources3
Beta strandi264 – 269Combined sources6
Beta strandi275 – 279Combined sources5
Beta strandi284 – 289Combined sources6
Helixi291 – 293Combined sources3
Beta strandi378 – 380Combined sources3
Beta strandi383 – 391Combined sources9
Beta strandi400 – 404Combined sources5
Helixi410 – 420Combined sources11
Helixi425 – 427Combined sources3
Helixi428 – 438Combined sources11
Helixi480 – 494Combined sources15
Helixi499 – 509Combined sources11
Helixi512 – 529Combined sources18
Helixi534 – 543Combined sources10
Helixi544 – 546Combined sources3
Helixi550 – 553Combined sources4
Helixi554 – 560Combined sources7
Helixi566 – 579Combined sources14
Turni583 – 585Combined sources3
Helixi586 – 590Combined sources5
Helixi593 – 596Combined sources4
Helixi599 – 602Combined sources4
Beta strandi608 – 610Combined sources3
Helixi612 – 634Combined sources23
Helixi642 – 651Combined sources10
Turni652 – 656Combined sources5
Helixi658 – 662Combined sources5
Helixi664 – 680Combined sources17
Helixi682 – 685Combined sources4
Turni688 – 690Combined sources3
Helixi692 – 701Combined sources10
Helixi705 – 714Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GAEX-ray1.60A/B464-715[»]
3L3FX-ray1.90X354-715[»]
3ODTX-ray1.35A/B2-300[»]
3PSPX-ray2.42A325-715[»]
3PSTX-ray2.00A325-715[»]
ProteinModelPortaliP36037
SMRiP36037
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36037

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 40WD 1Add BLAST30
Repeati53 – 82WD 2Add BLAST30
Repeati97 – 125WD 3Add BLAST29
Repeati135 – 166WD 4Add BLAST32
Repeati177 – 206WD 5Add BLAST30
Repeati218 – 247WD 6Add BLAST30
Repeati259 – 288WD 7Add BLAST30
Domaini352 – 449PFUPROSITE-ProRule annotationAdd BLAST98
Domaini465 – 715PULPROSITE-ProRule annotationAdd BLAST251
Repeati478 – 512ARM 1Add BLAST35
Repeati513 – 543ARM 2Add BLAST31
Repeati544 – 582ARM 3Add BLAST39
Repeati583 – 635ARM 4Add BLAST53
Repeati636 – 680ARM 5Add BLAST45
Repeati681 – 715ARM 6Add BLAST35

Domaini

The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with CDC48 C-terminus.
The PFU domain mediates interaction with ubiquitin.

Sequence similaritiesi

Belongs to the WD repeat PLAP family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00550000074944
HOGENOMiHOG000174247
InParanoidiP36037
KOiK14018
OMAiQEGSTIM
OrthoDBiEOG092C1BUL

Family and domain databases

Gene3Di1.10.150.410, 1 hit
1.25.10.10, 1 hit
2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR020472 G-protein_beta_WD-40_rep
IPR015155 PFU
IPR038122 PFU_sf
IPR033510 PLAA/Doa1/Lub1
IPR013535 PUL_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19849 PTHR19849, 1 hit
PfamiView protein in Pfam
PF09070 PFU, 1 hit
PF08324 PUL, 1 hit
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF48371 SSF48371, 1 hit
SSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS51394 PFU, 1 hit
PS51396 PUL, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P36037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV
60 70 80 90 100
YTGQGFLNSV CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG
110 120 130 140 150
NVCSLSFQDG VVISGSWDKT AKVWKEGSLV YNLQAHNASV WDAKVVSFSE
160 170 180 190 200
NKFLTASADK TIKLWQNDKV IKTFSGIHND VVRHLAVVDD GHFISCSNDG
210 220 230 240 250
LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED RTVRIWSKEN
260 270 280 290 300
GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI
310 320 330 340 350
NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI
360 370 380 390 400
EAHQFSNSSW KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL
410 420 430 440 450
KLPINVSDNP YTAADNFLAR YELPMSYRDQ VVQFILKNTN GISLDQPNDN
460 470 480 490 500
ASSSAVSPSK TSVMKVLPVK QYLIMENYNP DTIFNGIVKI NSNEKTFDDE
510 520 530 540 550
ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV RLIVKKLPYS
560 570 580 590 600
SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF
610 620 630 640 650
ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI
660 670 680 690 700
NTKYGPLEEY QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS
710
YGNVPRFKDI FDDLS
Length:715
Mass (Da):79,506
Last modified:June 1, 1994 - v1
Checksum:i593B808169283B5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180D → DI AA sequence (PubMed:15096053).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39947 Genomic DNA Translation: AAA82258.1
X75951 Genomic DNA Translation: CAA53560.1
Z28213 Genomic DNA Translation: CAA82058.1
BK006944 Genomic DNA Translation: DAA08956.1
PIRiS38051
RefSeqiNP_012709.1, NM_001179778.1

Genome annotation databases

EnsemblFungiiYKL213C; YKL213C; YKL213C
GeneIDi853667
KEGGisce:YKL213C

Similar proteinsi

Entry informationi

Entry nameiDOA1_YEAST
AccessioniPrimary (citable) accession number: P36037
Secondary accession number(s): D6VWZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health