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Protein

Protein DOA1

Gene

DOA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May act by preventing the interaction between CDC48 and the E4 enzyme UFD2, leading to prevent multiubiquitination of substrates and subsequent degradation. Essential for maintaining cellular ubiquitin levels.2 Publications

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • double-strand break repair via nonhomologous end joining Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • ribophagy Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin homeostasis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31971-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DOA1
Gene namesi
Name:DOA1
Synonyms:UFD3, ZZZ4
Ordered Locus Names:YKL213C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL213C.
SGDiS000001696. DOA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi541R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. 1 Publication1
Mutagenesisi669R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509581 – 715Protein DOA1Add BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei332PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36037.
PRIDEiP36037.

PTM databases

iPTMnetiP36037.

Interactioni

Subunit structurei

Interacts with CDC48, OTU1 and ubiquitin.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC48P256945EBI-6017,EBI-4308
HSE1P387533EBI-6017,EBI-1382
UBP3Q014774EBI-6017,EBI-19834

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33952. 470 interactors.
DIPiDIP-6274N.
IntActiP36037. 9 interactors.
MINTiMINT-606421.

Structurei

Secondary structure

1715
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Beta strandi16 – 23Combined sources8
Beta strandi26 – 31Combined sources6
Beta strandi34 – 52Combined sources19
Beta strandi57 – 63Combined sources7
Turni64 – 67Combined sources4
Beta strandi68 – 73Combined sources6
Beta strandi78 – 82Combined sources5
Beta strandi102 – 108Combined sources7
Beta strandi111 – 116Combined sources6
Beta strandi119 – 125Combined sources7
Beta strandi128 – 134Combined sources7
Beta strandi140 – 147Combined sources8
Turni148 – 151Combined sources4
Beta strandi152 – 157Combined sources6
Beta strandi162 – 166Combined sources5
Beta strandi169 – 174Combined sources6
Beta strandi182 – 189Combined sources8
Beta strandi192 – 197Combined sources6
Beta strandi200 – 206Combined sources7
Turni207 – 209Combined sources3
Beta strandi212 – 217Combined sources6
Beta strandi223 – 228Combined sources6
Beta strandi234 – 238Combined sources5
Beta strandi241 – 246Combined sources6
Turni248 – 250Combined sources3
Beta strandi253 – 258Combined sources6
Beta strandi260 – 262Combined sources3
Beta strandi264 – 269Combined sources6
Beta strandi275 – 279Combined sources5
Beta strandi284 – 289Combined sources6
Helixi291 – 293Combined sources3
Beta strandi378 – 380Combined sources3
Beta strandi383 – 391Combined sources9
Beta strandi400 – 404Combined sources5
Helixi410 – 420Combined sources11
Helixi425 – 427Combined sources3
Helixi428 – 438Combined sources11
Helixi480 – 494Combined sources15
Helixi499 – 509Combined sources11
Helixi512 – 529Combined sources18
Helixi534 – 543Combined sources10
Helixi544 – 546Combined sources3
Helixi550 – 553Combined sources4
Helixi554 – 560Combined sources7
Helixi566 – 579Combined sources14
Turni583 – 585Combined sources3
Helixi586 – 590Combined sources5
Helixi593 – 596Combined sources4
Helixi599 – 602Combined sources4
Beta strandi608 – 610Combined sources3
Helixi612 – 634Combined sources23
Helixi642 – 651Combined sources10
Turni652 – 656Combined sources5
Helixi658 – 662Combined sources5
Helixi664 – 680Combined sources17
Helixi682 – 685Combined sources4
Turni688 – 690Combined sources3
Helixi692 – 701Combined sources10
Helixi705 – 714Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GAEX-ray1.60A/B464-715[»]
3L3FX-ray1.90X354-715[»]
3ODTX-ray1.35A/B2-300[»]
3PSPX-ray2.42A325-715[»]
3PSTX-ray2.00A325-715[»]
ProteinModelPortaliP36037.
SMRiP36037.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36037.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 40WD 1Add BLAST30
Repeati53 – 82WD 2Add BLAST30
Repeati97 – 125WD 3Add BLAST29
Repeati135 – 166WD 4Add BLAST32
Repeati177 – 206WD 5Add BLAST30
Repeati218 – 247WD 6Add BLAST30
Repeati259 – 288WD 7Add BLAST30
Domaini352 – 449PFUPROSITE-ProRule annotationAdd BLAST98
Domaini465 – 715PULPROSITE-ProRule annotationAdd BLAST251
Repeati478 – 512ARM 1Add BLAST35
Repeati513 – 543ARM 2Add BLAST31
Repeati544 – 582ARM 3Add BLAST39
Repeati583 – 635ARM 4Add BLAST53
Repeati636 – 680ARM 5Add BLAST45
Repeati681 – 715ARM 6Add BLAST35

Domaini

The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with CDC48 C-terminus.
The PFU domain mediates interaction with ubiquitin.

Sequence similaritiesi

Belongs to the WD repeat PLAP family.Curated
Contains 6 ARM repeats.Curated
Contains 1 PFU domain.PROSITE-ProRule annotation
Contains 1 PUL domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00550000074944.
HOGENOMiHOG000174247.
InParanoidiP36037.
KOiK14018.
OMAiQWSGGEW.
OrthoDBiEOG092C1BUL.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR020472. G-protein_beta_WD-40_rep.
IPR033510. PLAA/Doa1/Lub1.
IPR015155. PLAA_fam_Ub-bd_PFU.
IPR013535. PUL_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19849:SF0. PTHR19849:SF0. 1 hit.
PfamiPF09070. PFU. 1 hit.
PF08324. PUL. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS51394. PFU. 1 hit.
PS51396. PUL. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV
60 70 80 90 100
YTGQGFLNSV CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG
110 120 130 140 150
NVCSLSFQDG VVISGSWDKT AKVWKEGSLV YNLQAHNASV WDAKVVSFSE
160 170 180 190 200
NKFLTASADK TIKLWQNDKV IKTFSGIHND VVRHLAVVDD GHFISCSNDG
210 220 230 240 250
LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED RTVRIWSKEN
260 270 280 290 300
GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI
310 320 330 340 350
NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI
360 370 380 390 400
EAHQFSNSSW KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL
410 420 430 440 450
KLPINVSDNP YTAADNFLAR YELPMSYRDQ VVQFILKNTN GISLDQPNDN
460 470 480 490 500
ASSSAVSPSK TSVMKVLPVK QYLIMENYNP DTIFNGIVKI NSNEKTFDDE
510 520 530 540 550
ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV RLIVKKLPYS
560 570 580 590 600
SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF
610 620 630 640 650
ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI
660 670 680 690 700
NTKYGPLEEY QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS
710
YGNVPRFKDI FDDLS
Length:715
Mass (Da):79,506
Last modified:June 1, 1994 - v1
Checksum:i593B808169283B5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180D → DI AA sequence (PubMed:15096053).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39947 Genomic DNA. Translation: AAA82258.1.
X75951 Genomic DNA. Translation: CAA53560.1.
Z28213 Genomic DNA. Translation: CAA82058.1.
BK006944 Genomic DNA. Translation: DAA08956.1.
PIRiS38051.
RefSeqiNP_012709.1. NM_001179778.1.

Genome annotation databases

EnsemblFungiiYKL213C; YKL213C; YKL213C.
GeneIDi853667.
KEGGisce:YKL213C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39947 Genomic DNA. Translation: AAA82258.1.
X75951 Genomic DNA. Translation: CAA53560.1.
Z28213 Genomic DNA. Translation: CAA82058.1.
BK006944 Genomic DNA. Translation: DAA08956.1.
PIRiS38051.
RefSeqiNP_012709.1. NM_001179778.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GAEX-ray1.60A/B464-715[»]
3L3FX-ray1.90X354-715[»]
3ODTX-ray1.35A/B2-300[»]
3PSPX-ray2.42A325-715[»]
3PSTX-ray2.00A325-715[»]
ProteinModelPortaliP36037.
SMRiP36037.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33952. 470 interactors.
DIPiDIP-6274N.
IntActiP36037. 9 interactors.
MINTiMINT-606421.

PTM databases

iPTMnetiP36037.

Proteomic databases

MaxQBiP36037.
PRIDEiP36037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL213C; YKL213C; YKL213C.
GeneIDi853667.
KEGGisce:YKL213C.

Organism-specific databases

EuPathDBiFungiDB:YKL213C.
SGDiS000001696. DOA1.

Phylogenomic databases

GeneTreeiENSGT00550000074944.
HOGENOMiHOG000174247.
InParanoidiP36037.
KOiK14018.
OMAiQWSGGEW.
OrthoDBiEOG092C1BUL.

Enzyme and pathway databases

BioCyciYEAST:G3O-31971-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP36037.
PROiP36037.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR020472. G-protein_beta_WD-40_rep.
IPR033510. PLAA/Doa1/Lub1.
IPR015155. PLAA_fam_Ub-bd_PFU.
IPR013535. PUL_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19849:SF0. PTHR19849:SF0. 1 hit.
PfamiPF09070. PFU. 1 hit.
PF08324. PUL. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS51394. PFU. 1 hit.
PS51396. PUL. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOA1_YEAST
AccessioniPrimary (citable) accession number: P36037
Secondary accession number(s): D6VWZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.