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P36037 (DOA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein DOA1
Gene names
Name:DOA1
Synonyms:UFD3, ZZZ4
Ordered Locus Names:YKL213C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May act by preventing the interaction between CDC48 and the E4 enzyme UFD2, leading to prevent multiubiquitination of substrates and subsequent degradation. Essential for maintaining cellular ubiquitin levels. Ref.5 Ref.6

Subunit structure

Interacts with CDC48, OTU1 and ubiquitin. Ref.9 Ref.10 Ref.14

Subcellular location

Nucleus. Cytoplasm Ref.7.

Domain

The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with CDC48 C-terminus.

The PFU domain mediates interaction with ubiquitin.

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat PLAP family.

Contains 6 ARM repeats.

Contains 1 PFU domain.

Contains 1 PUL domain.

Contains 7 WD repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 715715Protein DOA1
PRO_0000050958

Regions

Repeat11 – 4030WD 1
Repeat53 – 8230WD 2
Repeat97 – 12529WD 3
Repeat135 – 16632WD 4
Repeat177 – 20630WD 5
Repeat218 – 24730WD 6
Repeat259 – 28830WD 7
Domain352 – 44998PFU
Domain465 – 715251PUL
Repeat478 – 51235ARM 1
Repeat513 – 54331ARM 2
Repeat544 – 58239ARM 3
Repeat583 – 63553ARM 4
Repeat636 – 68045ARM 5
Repeat681 – 71535ARM 6

Amino acid modifications

Modified residue3321Phosphoserine Ref.11 Ref.12

Experimental info

Mutagenesis5411R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Ref.14
Mutagenesis6691R → A: Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Ref.14
Sequence conflict1801D → DI AA sequence Ref.5

Secondary structure

............................................................................................................... 715
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36037 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 593B808169283B5F

FASTA71579,506
        10         20         30         40         50         60 
MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV YTGQGFLNSV 

        70         80         90        100        110        120 
CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG NVCSLSFQDG VVISGSWDKT 

       130        140        150        160        170        180 
AKVWKEGSLV YNLQAHNASV WDAKVVSFSE NKFLTASADK TIKLWQNDKV IKTFSGIHND 

       190        200        210        220        230        240 
VVRHLAVVDD GHFISCSNDG LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED 

       250        260        270        280        290        300 
RTVRIWSKEN GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI 

       310        320        330        340        350        360 
NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI EAHQFSNSSW 

       370        380        390        400        410        420 
KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL KLPINVSDNP YTAADNFLAR 

       430        440        450        460        470        480 
YELPMSYRDQ VVQFILKNTN GISLDQPNDN ASSSAVSPSK TSVMKVLPVK QYLIMENYNP 

       490        500        510        520        530        540 
DTIFNGIVKI NSNEKTFDDE ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV 

       550        560        570        580        590        600 
RLIVKKLPYS SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF 

       610        620        630        640        650        660 
ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI NTKYGPLEEY 

       670        680        690        700        710 
QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS YGNVPRFKDI FDDLS 

« Hide

References

« Hide 'large scale' references
[1]Hochstrasser M., Gang G.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein."
Tzermia M., Horaitis O., Alexandraki D.
Yeast 10:663-679(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Identification of a novel 29-linked polyubiquitin binding protein, Ufd3, using polyubiquitin chain analogues."
Russell N.S., Wilkinson K.D.
Biochemistry 43:4844-4854(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 126-144; 173-183; 215-227; 316-335; 345-361; 384-420; 534-541; 598-613; 654-685 AND 700-706, FUNCTION.
[6]"Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae."
Ghislain M., Dohmen R.J., Levy F., Varshavsky A.
EMBO J. 15:4884-4899(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone."
Rumpf S., Jentsch S.
Mol. Cell 21:261-269(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OTU1 AND CDC48.
[10]"Doa1 is a Cdc48 adapter that possesses a novel ubiquitin binding domain."
Mullally J.E., Chernova T., Wilkinson K.D.
Mol. Cell. Biol. 26:822-830(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBIQUITIN AND CDC48.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"An Armadillo motif in Ufd3 interacts with Cdc48 and is involved in ubiquitin homeostasis and protein degradation."
Zhao G., Li G., Schindelin H., Lennarz W.J.
Proc. Natl. Acad. Sci. U.S.A. 106:16197-16202(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 464-715, INTERACTION WITH CDC48, ARM REPEATS, MUTAGENESIS OF ARG-541 AND ARG-669.
[15]"Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3."
Nishimasu R., Komori H., Higuchi Y., Nishimasu H., Hiroaki H.
Kobe J. Med. Sci. 56:E125-E139(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 354-715, ARM REPEATS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39947 Genomic DNA. Translation: AAA82258.1.
X75951 Genomic DNA. Translation: CAA53560.1.
Z28213 Genomic DNA. Translation: CAA82058.1.
BK006944 Genomic DNA. Translation: DAA08956.1.
PIRS38051.
RefSeqNP_012709.1. NM_001179778.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GAEX-ray1.60A/B464-715[»]
3L3FX-ray1.90X354-715[»]
3ODTX-ray1.35A/B2-300[»]
3PSPX-ray2.42A325-715[»]
3PSTX-ray2.00A325-715[»]
ProteinModelPortalP36037.
SMRP36037. Positions 2-295, 376-715.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33952. 437 interactions.
DIPDIP-6274N.
IntActP36037. 9 interactions.
MINTMINT-606421.
STRING4932.YKL213C.

Proteomic databases

MaxQBP36037.
PaxDbP36037.
PeptideAtlasP36037.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL213C; YKL213C; YKL213C.
GeneID853667.
KEGGsce:YKL213C.

Organism-specific databases

CYGDYKL213c.
SGDS000001696. DOA1.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00550000074944.
HOGENOMHOG000174247.
KOK14018.
OMAPAYDIVR.
OrthoDBEOG7FR7QW.

Enzyme and pathway databases

BioCycYEAST:G3O-31971-MONOMER.

Gene expression databases

GenevestigatorP36037.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR016024. ARM-type_fold.
IPR020472. G-protein_beta_WD-40_rep.
IPR015155. PLAA_fam_Ub-bd_PFU.
IPR013535. PUL.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF09070. PFU. 1 hit.
PF08324. PUL. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEPS51394. PFU. 1 hit.
PS51396. PUL. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP36037.
NextBio974605.
PROP36037.

Entry information

Entry nameDOA1_YEAST
AccessionPrimary (citable) accession number: P36037
Secondary accession number(s): D6VWZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references