Reviewed, UniProtKB/Swiss-Prot P36036 (YKV4_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized RNA-binding protein YKL214C

Gene names

Ordered Locus Names:

YKL214C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 1310 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	poly(A)+ mRNA export from nucleus Inferred from genetic interaction. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GPM2	Q12008	1	EBI-27015,EBI-2063839
LSB3	P43603	1	EBI-27015,EBI-22980
YSC84	P32793	1	EBI-27015,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Uncharacterized RNA-binding protein YKL214C		PRO_0000082033
Regions
	Domain	64 – 138	75	RRM
Amino acid modifications
	Modified residue	186	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36036-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 89B7A4B210438652	FASTA	203	23,778
	10 20 30 40 50 60 MDKAFDEIIG NSHTDSSSNH KVTRYRRRDL RNELGPRLGF APSDAASRSK DRLYREREEP 70 80 90 100 110 120 PLPKRIRISK IPLDVSDYTL DDMIKEFGSP IFSKIFDNKE DRTCIYEFED PEVLEKIVER 130 140 150 160 170 180 YNGHELHNAK IEVEIYQPQR KHSRMNAHNR RKQTAQEHGR GRPGSHYRQK PNRVSKKNKG 190 200 REKNNTPTSV EALDAELDAY MKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed: 7941750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75951 Genomic DNA. Translation: CAA53559.1. Z28214 Genomic DNA. Translation: CAA82059.1.
PIR	S38052.
RefSeq	NP_012708.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6538N.
IntAct	P36036. 8 interactions.
STRING	P36036.
Proteomic databases
PeptideAtlas	P36036.
PRIDE	P36036.
Genome annotation databases
Ensembl	YKL214C; YKL214C; YKL214C; Saccharomyces cerevisiae. [Genome view]
GeneID	853666.
KEGG	sce:YKL214C.
NMPDR	fig|4932.3.peg.3686.
Organism-specific databases
CYGD	YKL214c.
SGD	S000001697. YRA2.
Phylogenomic databases
HOGENOM	P36036.
OMA	EDWINEI
OrthoDB	EOG9WM6B1
Gene expression databases
ArrayExpress	P36036.
Genevestigator	P36036.
GermOnline	YKL214C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974602.

Entry information

Entry name

YKV4_YEAST

Accession

Primary (citable) accession number: P36036

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)