P36036 (YRA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA annealing protein YRA2

Gene names

Name:	YRA2
Ordered Locus Names:	YKL214C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in export of poly(A) mRNAs from the nucleus. Recruited to the coding sequences as well as poly-A sites of active genes. Ref.4 Ref.6
Subunit structure	Associates with mRNPs. Interacts with YRA1. Ref.6
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 1310 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the YRA1 family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Transport mRNA transport
Cellular component	Nucleus
Ligand	DNA-binding RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	poly(A)+ mRNA export from nucleus Inferred from genetic interaction Ref.4. Source: SGD
Cellular component	nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from direct assay Ref.4. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	RNA annealing protein YRA2		PRO_0000082033
Regions
	Domain	64 – 138	75	RRM
Amino acid modifications
	Modified residue	186	1	Phosphothreonine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P36036 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 89B7A4B210438652	FASTA	203	23,778
	10 20 30 40 50 60 MDKAFDEIIG NSHTDSSSNH KVTRYRRRDL RNELGPRLGF APSDAASRSK DRLYREREEP 70 80 90 100 110 120 PLPKRIRISK IPLDVSDYTL DDMIKEFGSP IFSKIFDNKE DRTCIYEFED PEVLEKIVER 130 140 150 160 170 180 YNGHELHNAK IEVEIYQPQR KHSRMNAHNR RKQTAQEHGR GRPGSHYRQK PNRVSKKNKG 190 200 REKNNTPTSV EALDAELDAY MKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed: 7941750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast hnRNP-Like proteins Yra1p and Yra2p participate in mRNA export through interaction with Mex67p." Zenklusen D., Vinciguerra P., Strahm Y., Stutz F. Mol. Cell. Biol. 21:4219-4232(2001) [PubMed: 11390651] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH MRNPS, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Biochemical and genetic characterization of Yra1p in budding yeast." Kashyap A.K., Schieltz D., Yates J. III, Kellogg D.R. Yeast 22:43-56(2005) [PubMed: 15584090] [Abstract] Cited for: FUNCTION, INTERACTION WITH YRA1.
[7]	"Comprehensive analysis of diverse ribonucleoprotein complexes." Oeffinger M., Wei K.E., Rogers R., DeGrasse J.A., Chait B.T., Aitchison J.D., Rout M.P. Nat. Methods 4:951-956(2007) [PubMed: 17922018] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH MRNPS.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186, MASS SPECTROMETRY.
[9]	"Stimulation of mRNA export by an F-box protein, Mdm30p, in vivo." Shukla A., Durairaj G., Schneider J., Duan Z., Shadle T., Bhaumik S.R. J. Mol. Biol. 389:238-247(2009) [PubMed: 19376128] [Abstract] Cited for: DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75951 Genomic DNA. Translation: CAA53559.1. Z28214 Genomic DNA. Translation: CAA82059.1. BK006944 Genomic DNA. Translation: DAA08955.1.
PIR	S38052.
RefSeq	NP_012708.1. NM_001179779.1.
3D structure databases
ProteinModelPortal	P36036.
SMR	P36036. Positions 60-138.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6538N.
IntAct	P36036. 8 interactions.
MINT	MINT-602129.
STRING	P36036.
Proteomic databases
PeptideAtlas	P36036.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL214C; YKL214C; YKL214C.
GeneID	853666.
KEGG	sce:YKL214C.
NMPDR	fig|4932.3.peg.3686.
Organism-specific databases
CYGD	YKL214c.
SGD	S000001697. YRA2.
Phylogenomic databases
eggNOG	fuNOG11950.
HOGENOM	HBG202301.
OMA	EDWINEI.
OrthoDB	EOG403132.
Gene expression databases
ArrayExpress	P36036.
Genevestigator	P36036.
GermOnline	YKL214C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974602.

Entry information

Entry name

YRA2_YEAST

Accession

Primary (citable) accession number: P36036
Secondary accession number(s): D6VWY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program