Reviewed, UniProtKB/Swiss-Prot P36035 (JEN1_YEAST)

Names and origin

Protein names

Recommended name:
    Carboxylic acid transporter protein homolog

Gene names

Name:	JEN1
Ordered Locus Names:	YKL217W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential to lactate transport.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactate transport Inferred from direct assay. Source: SGD transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: SGD plasma membrane Inferred from mutant phenotype. Source: SGD
Molecular function	carbohydrate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro lactate transmembrane transporter activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Carboxylic acid transporter protein homolog		PRO_0000050460
Regions
	Topological domain	1 – 140	140	Cytoplasmic Potential
	Transmembrane	141 – 161	21	1 Potential
	Topological domain	162 – 176	15	Extracellular Potential
	Transmembrane	177 – 197	21	2 Potential
	Topological domain	198 – 205	8	Cytoplasmic Potential
	Transmembrane	206 – 226	21	3 Potential
	Topological domain	227 – 230	4	Extracellular Potential
	Transmembrane	231 – 251	21	4 Potential
	Topological domain	252 – 263	12	Cytoplasmic Potential
	Transmembrane	264 – 284	21	5 Potential
	Topological domain	285 – 296	12	Extracellular Potential
	Transmembrane	297 – 317	21	6 Potential
	Topological domain	318 – 363	46	Cytoplasmic Potential
	Transmembrane	364 – 384	21	7 Potential
	Topological domain	385 – 402	18	Extracellular Potential
	Transmembrane	403 – 423	21	8 Potential
	Topological domain	424 – 432	9	Cytoplasmic Potential
	Transmembrane	433 – 453	21	9 Potential
	Topological domain	454 – 457	4	Extracellular Potential
	Transmembrane	458 – 478	21	10 Potential
	Topological domain	479 – 489	11	Cytoplasmic Potential
	Transmembrane	490 – 510	21	11 Potential
	Topological domain	511 – 535	25	Extracellular Potential
	Transmembrane	536 – 556	21	12 Potential
	Topological domain	557 – 616	60	Cytoplasmic Potential
Amino acid modifications
	Modified residue	4	1	Phosphoserine Ref.6
	Modified residue	11	1	Phosphoserine Ref.6
	Modified residue	81	1	Phosphoserine Ref.6 Ref.4
	Modified residue	584	1	Phosphoserine Ref.6
	Modified residue	606	1	Phosphoserine Ref.6
	Cross-link	9		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4
	Cross-link	338		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36035-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 47926F50842ACD5B	FASTA	616	69,376
	10 20 30 40 50 60 MSSSITDEKI SGEQQQPAGR KLYYNTSTFA EPPLVDGEGN PINYEPEVYN PDHEKLYHNP 70 80 90 100 110 120 SLPAQSIQDT RDDELLERVY SQDQGVEYEE DEEDKPNLSA ASIKSYALTR FTSLLHIHEF 130 140 150 160 170 180 SWENVNPIPE LRKMTWQNWN YFFMGYFAWL SAAWAFFCVS VSVAPLAELY DRPTKDITWG 190 200 210 220 230 240 LGLVLFVRSA GAVIFGLWTD KSSRKWPYIT CLFLFVIAQL CTPWCDTYEK FLGVRWITGI 250 260 270 280 290 300 AMGGIYGCAS ATAIEDAPVK ARSFLSGLFF SAYAMGFIFA IIFYRAFGYF RDDGWKILFW 310 320 330 340 350 360 FSIFLPILLI FWRLLWPETK YFTKVLKARK LILSDAVKAN GGEPLPKANF KQKMVSMKRT 370 380 390 400 410 420 VQKYWLLFAY LVVLLVGPNY LTHASQDLLP TMLRAQLGLS KDAVTVIVVV TNIGAICGGM 430 440 450 460 470 480 IFGQFMEVTG RRLGLLIACT MGGCFTYPAF MLRSEKAILG AGFMLYFCVF GVWGILPIHL 490 500 510 520 530 540 AELAPADARA LVAGLSYQLG NLASAAASTI ETQLADRYPL ERDASGAVIK EDYAKVMAIL 550 560 570 580 590 600 TGSVFIFTFA CVFVGHEKFH RDLSSPVMKK YINQVEEYEA DGLSISDIVE QKTECASVKM 610 IDSNVSKTYE EHIETV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Davis E.S., Brennan M.B. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[2]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed: 7941750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, UBIQUITINATION AT LYS-9 AND LYS-338, MASS SPECTROMETRY.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[6]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-11; SER-81; SER-584 AND SER-606, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U24155 Genomic DNA. Translation: AAB60291.1. X75951 Genomic DNA. Translation: CAA53556.1. Z28217 Genomic DNA. Translation: CAA82062.1.
PIR	S38060.
RefSeq	NP_012705.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:8064N.
STRING	P36035.
Protein family/group databases
TCDB	2.A.1.12.2. major facilitator superfamily (MFS).
Proteomic databases
PeptideAtlas	P36035.
Genome annotation databases
Ensembl	YKL217W; YKL217W; YKL217W; Saccharomyces cerevisiae. [Genome view]
GeneID	853663.
KEGG	sce:YKL217W.
NMPDR	fig|4932.3.peg.3683.
Organism-specific databases
CYGD	YKL217w.
SGD	S000001700. JEN1.
Phylogenomic databases
HOGENOM	P36035.
OMA	NYLTHAS
OrthoDB	EOG9HQG1V
Gene expression databases
ArrayExpress	P36035.
Genevestigator	P36035.
GermOnline	YKL217W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016196. MFS_general_subst_transpt. IPR004742. SA_transporter. IPR005828. Sub_transporter. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00891. 2A0112. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. False negative. PS00217. SUGAR_TRANSPORT_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974593.

Entry information

Entry name

JEN1_YEAST

Accession

Primary (citable) accession number: P36035

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)