Carboxylic acid transporter protein homolog - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36035 (JEN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Carboxylic acid transporter protein homolog

Gene names

Name:	JEN1
Ordered Locus Names:	YKL217W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential to lactate transport.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	plasma membrane lactate transport Inferred from mutant phenotype PubMed 10198029 PubMed 17710650. Source: SGD plasma membrane pyruvate transport Inferred from mutant phenotype PubMed 17710650. Source: SGD plasma membrane selenite transport Inferred from mutant phenotype PubMed 20861301. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD plasma membrane Inferred from direct assay PubMed 17710650. Source: SGD
Molecular_function	carbohydrate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro secondary active monocarboxylate transmembrane transporter activity Inferred from mutant phenotype PubMed 17710650. Source: SGD selenite:hydrogen symporter activity Inferred from mutant phenotype PubMed 20861301. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 616

616

Carboxylic acid transporter protein homolog

PRO_0000050460

Regions

Topological domain

1 – 140

140

Cytoplasmic Potential

Transmembrane

141 – 161

Helical; Name=1; Potential

Topological domain

162 – 176

Extracellular Potential

Transmembrane

177 – 197

Helical; Name=2; Potential

Topological domain

198 – 205

Cytoplasmic Potential

Transmembrane

206 – 226

Helical; Name=3; Potential

Topological domain

227 – 230

Extracellular Potential

Transmembrane

231 – 251

Helical; Name=4; Potential

Topological domain

252 – 263

Cytoplasmic Potential

Transmembrane

264 – 284

Helical; Name=5; Potential

Topological domain

285 – 296

Extracellular Potential

Transmembrane

297 – 317

Helical; Name=6; Potential

Topological domain

318 – 363

Cytoplasmic Potential

Transmembrane

364 – 384

Helical; Name=7; Potential

Topological domain

385 – 402

Extracellular Potential

Transmembrane

403 – 423

Helical; Name=8; Potential

Topological domain

424 – 432

Cytoplasmic Potential

Transmembrane

433 – 453

Helical; Name=9; Potential

Topological domain

454 – 457

Extracellular Potential

Transmembrane

458 – 478

Helical; Name=10; Potential

Topological domain

479 – 489

Cytoplasmic Potential

Transmembrane

490 – 510

Helical; Name=11; Potential

Topological domain

511 – 535

Extracellular Potential

Transmembrane

536 – 556

Helical; Name=12; Potential

Topological domain

557 – 616

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphoserine Ref.5 Ref.7

Modified residue

584

Phosphoserine Ref.7

Modified residue

606

Phosphoserine Ref.7

Cross-link

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Cross-link

338

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P36035 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 47926F50842ACD5B
Show »

FASTA

616

69,376

        10         20         30         40         50         60 
MSSSITDEKI SGEQQQPAGR KLYYNTSTFA EPPLVDGEGN PINYEPEVYN PDHEKLYHNP 

        70         80         90        100        110        120 
SLPAQSIQDT RDDELLERVY SQDQGVEYEE DEEDKPNLSA ASIKSYALTR FTSLLHIHEF 

       130        140        150        160        170        180 
SWENVNPIPE LRKMTWQNWN YFFMGYFAWL SAAWAFFCVS VSVAPLAELY DRPTKDITWG 

       190        200        210        220        230        240 
LGLVLFVRSA GAVIFGLWTD KSSRKWPYIT CLFLFVIAQL CTPWCDTYEK FLGVRWITGI 

       250        260        270        280        290        300 
AMGGIYGCAS ATAIEDAPVK ARSFLSGLFF SAYAMGFIFA IIFYRAFGYF RDDGWKILFW 

       310        320        330        340        350        360 
FSIFLPILLI FWRLLWPETK YFTKVLKARK LILSDAVKAN GGEPLPKANF KQKMVSMKRT 

       370        380        390        400        410        420 
VQKYWLLFAY LVVLLVGPNY LTHASQDLLP TMLRAQLGLS KDAVTVIVVV TNIGAICGGM 

       430        440        450        460        470        480 
IFGQFMEVTG RRLGLLIACT MGGCFTYPAF MLRSEKAILG AGFMLYFCVF GVWGILPIHL 

       490        500        510        520        530        540 
AELAPADARA LVAGLSYQLG NLASAAASTI ETQLADRYPL ERDASGAVIK EDYAKVMAIL 

       550        560        570        580        590        600 
TGSVFIFTFA CVFVGHEKFH RDLSSPVMKK YINQVEEYEA DGLSISDIVE QKTECASVKM 

       610 
IDSNVSKTYE EHIETV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Davis E.S., Brennan M.B. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[2]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, UBIQUITINATION AT LYS-9 AND LYS-338, MASS SPECTROMETRY. Strain: SUB592.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-11; SER-81; SER-584 AND SER-606, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24155 Genomic DNA. Translation: AAB60291.1. X75951 Genomic DNA. Translation: CAA53556.1. Z28217 Genomic DNA. Translation: CAA82062.1. BK006944 Genomic DNA. Translation: DAA08952.1.
PIR	S38060.
RefSeq	NP_012705.1. NM_001179782.1.
3D structure databases
ProteinModelPortal	P36035.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8064N.
STRING	4932.YKL217W.
Protein family/group databases
TCDB	2.A.1.12.2. major facilitator superfamily (MFS).
Proteomic databases
PaxDb	P36035.
PeptideAtlas	P36035.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL217W; YKL217W; YKL217W.
GeneID	853663.
KEGG	sce:YKL217W.
Organism-specific databases
CYGD	YKL217w.
SGD	S000001700. JEN1.
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000259757.
KO	K08178.
OMA	NYLTHAS.
OrthoDB	EOG4N8VDD.
Gene expression databases
Genevestigator	P36035.
GermOnline	YKL217W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004742. SA_transporter. IPR005828. Sub_transporter. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00891. 2A0112. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. False negative. PS00217. SUGAR_TRANSPORT_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	974593.

Entry information

Entry name

JEN1_YEAST

Accession

Primary (citable) accession number: P36035
Secondary accession number(s): D6VWY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents