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Protein

Ferric/cupric reductase transmembrane component 2

Gene

FRE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloreductase responsible for reducing extracellular iron and copper prior to import. Catalyzes the reductive uptake of Fe3+-salts and Fe3+ bound to catecholate or hydroxamate siderophores. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane. Also participates in Cu2+ reduction and Cu+ uptake.2 Publications

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi316Iron (heme 1 axial ligand)By similarity1
Metal bindingi330Iron (heme 2 axial ligand)By similarity1
Metal bindingi386Iron (heme 1 axial ligand)By similarity1
Metal bindingi400Iron (heme 2 axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi479 – 485FADSequence analysis7
Nucleotide bindingi526 – 529NADPSequence analysis4
Nucleotide bindingi677 – 678NADPSequence analysis2

GO - Molecular functioni

GO - Biological processi

  • cellular iron ion homeostasis Source: GO_Central
  • copper ion import Source: SGD
  • iron ion transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Copper transport, Electron transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

Copper, FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:YKL220C-MONOMER.

Protein family/group databases

TCDBi5.B.1.5.3. the phagocyte (gp91(phox)) nadph oxidase family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric/cupric reductase transmembrane component 2 (EC:1.16.1.9)
Alternative name(s):
Ferric-chelate reductase 2
Gene namesi
Name:FRE2
Ordered Locus Names:YKL220C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL220C.
SGDiS000001703. FRE2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 164ExtracellularSequence analysisAdd BLAST141
Transmembranei165 – 185Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini186 – 235CytoplasmicSequence analysisAdd BLAST50
Transmembranei236 – 256Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini257 – 280ExtracellularSequence analysisAdd BLAST24
Transmembranei281 – 301Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini302 – 317CytoplasmicSequence analysisAdd BLAST16
Transmembranei318 – 340Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini341 – 353ExtracellularSequence analysisAdd BLAST13
Transmembranei354 – 374Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini375 – 377CytoplasmicSequence analysis3
Transmembranei378 – 398Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini399 – 400ExtracellularSequence analysis2
Transmembranei401 – 423Helical; Name=7Sequence analysisAdd BLAST23
Topological domaini424 – 711CytoplasmicSequence analysisAdd BLAST288

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001013824 – 711Ferric/cupric reductase transmembrane component 2Add BLAST688

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Glycosylationi108N-linked (GlcNAc...)Sequence analysis1
Glycosylationi120N-linked (GlcNAc...)Sequence analysis1
Glycosylationi134N-linked (GlcNAc...)Sequence analysis1
Glycosylationi341N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP36033.

Expressioni

Inductioni

By transcription factors AFT1 and AFT2 upon iron deprivation.6 Publications

Interactioni

Protein-protein interaction databases

BioGridi33945. 6 interactors.
DIPiDIP-7473N.
IntActiP36033. 1 interactor.
MINTiMINT-1363650.

Structurei

3D structure databases

ProteinModelPortaliP36033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 414Ferric oxidoreductaseAdd BLAST135
Domaini415 – 534FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST120

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiP36033.
OMAiPPNERCV.
OrthoDBiEOG092C04QA.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWTSILSAI LLFCLSGARA SPAKTVIRNK VPLLVTNACT RIFQKVTWEY
60 70 80 90 100
TSKSKRSSPV CSYEPAFQSM LYCIYETLDE KGYSNKTLEK TFSTIKKNCA
110 120 130 140 150
SYSDALQNMT NSEFYDVLNN GTRHMTPYVK GSANLTYPVE MDTQLRKAYY
160 170 180 190 200
HALHGFYANL DVGNIYGGII CAYFVAIMAF AGVLHCMNYT PFKTVLLKQK
210 220 230 240 250
LVGYVRGYLT LPTIGSKHAS DFSYFRIFTG YLPTRLEGII ILGYLVLHTV
260 270 280 290 300
FLAYGYEYDP ENIIFKSRRV QVARYVADRS GVLAFAHFPL IVLFAGRNNF
310 320 330 340 350
LEYISGVKYT SFIMFHKWLG RMMFLDAMIH GSAYTSYTVA NKTWATSKNR
360 370 380 390 400
LYWQFGVAAL CLAGTMVFFS FAVFRKYFYE AFLFLHIVLG AMFFYACWEH
410 420 430 440 450
VVSLSGIEWI YTAIAIWIVD RIIRIIKASY FGFPKASLQL IGDDLIRLTV
460 470 480 490 500
KKPARPWRAK PGQYVFVSFL HPLYFWQSHP FTVLDSVSKN GELVIILKEK
510 520 530 540 550
KGVTRLVKKY VCRNGGKTSM RLAIEGPYGS SSPVNNYNNV LLLTGGTGLP
560 570 580 590 600
GPIAHAIKLG KTSAAAGKQS VKLVIAVRGF DVLEAYKPEL MCLENLNVQL
610 620 630 640 650
HIYNTMEVPS LTPSDSLDIS QQDEKADEKG TVVATTLEKS ANPLGFDGVV
660 670 680 690 700
FHCGRPNVKE LLHEAAELSG SLSVVCCGPP IFVDKVRNET AKIVLDKSAK
710
AIEYFEEYQC W
Length:711
Mass (Da):80,072
Last modified:June 1, 1994 - v1
Checksum:i3C60346577C80E53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75950 Genomic DNA. Translation: CAA53553.1.
Z28220 Genomic DNA. Translation: CAA82065.1.
BK006944 Genomic DNA. Translation: DAA08949.1.
PIRiS38063.
RefSeqiNP_012702.1. NM_001179785.1.

Genome annotation databases

EnsemblFungiiYKL220C; YKL220C; YKL220C.
GeneIDi853660.
KEGGisce:YKL220C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75950 Genomic DNA. Translation: CAA53553.1.
Z28220 Genomic DNA. Translation: CAA82065.1.
BK006944 Genomic DNA. Translation: DAA08949.1.
PIRiS38063.
RefSeqiNP_012702.1. NM_001179785.1.

3D structure databases

ProteinModelPortaliP36033.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33945. 6 interactors.
DIPiDIP-7473N.
IntActiP36033. 1 interactor.
MINTiMINT-1363650.

Protein family/group databases

TCDBi5.B.1.5.3. the phagocyte (gp91(phox)) nadph oxidase family.

Proteomic databases

PRIDEiP36033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL220C; YKL220C; YKL220C.
GeneIDi853660.
KEGGisce:YKL220C.

Organism-specific databases

EuPathDBiFungiDB:YKL220C.
SGDiS000001703. FRE2.

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiP36033.
OMAiPPNERCV.
OrthoDBiEOG092C04QA.

Enzyme and pathway databases

BioCyciYEAST:YKL220C-MONOMER.

Miscellaneous databases

PROiP36033.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRE2_YEAST
AccessioniPrimary (citable) accession number: P36033
Secondary accession number(s): D6VWY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.