Reviewed, UniProtKB/Swiss-Prot P36032 (MCH2_YEAST)

Names and origin

Protein names

Recommended name:
    Probable transporter MCH2

Gene names

Name:	MCH2
Ordered Locus Names:	YKL221W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transporter. Does not act in the transport of monocarboxylic acids across the plasma membrane. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Domain	Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Probable transporter MCH2		PRO_0000211403
Regions
	Topological domain	1 – 37	37	Cytoplasmic Potential
	Transmembrane	38 – 58	21	Potential
	Topological domain	59 – 83	25	Extracellular Potential
	Transmembrane	84 – 105	22	Potential
	Topological domain	106 – 111	6	Cytoplasmic Potential
	Transmembrane	112 – 135	24	Potential
	Topological domain	136 – 141	6	Extracellular Potential
	Transmembrane	142 – 163	22	Potential
	Topological domain	164 – 169	6	Cytoplasmic Potential
	Transmembrane	170 – 186	17	Potential
	Topological domain	187 – 200	14	Extracellular Potential
	Transmembrane	201 – 220	20	Potential
	Topological domain	221 – 243	23	Cytoplasmic Potential
	Transmembrane	244 – 268	25	Potential
	Topological domain	269 – 286	18	Extracellular Potential
	Transmembrane	287 – 304	18	Potential
	Topological domain	305 – 312	8	Cytoplasmic Potential
	Transmembrane	313 – 332	20	Potential
	Topological domain	333 – 342	10	Extracellular Potential
	Transmembrane	343 – 362	20	Potential
	Topological domain	363 – 370	8	Cytoplasmic Potential
	Transmembrane	371 – 394	24	Potential
	Topological domain	395 – 408	14	Extracellular Potential
	Transmembrane	409 – 433	25	Potential
	Topological domain	434 – 473	40	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P36032-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6C4F4AD574B28510	FASTA	473	52,342
	10 20 30 40 50 60 MSEERHEDHH RDVENKLNLN GKDDINGNTS ISIEVPDGGY GWFILLAFIL YNFSTWGANS 70 80 90 100 110 120 GYAIYLAHYL ENNTFAGGSK LDYASIGGLA FSCGLFFAPV ITWLYHIFSI QFIIGLGILF 130 140 150 160 170 180 QGAALLLAAF SVTLWEIYLT QGVLIGFGLA FIFIPSVTLI PLWFRNKRSL ASGIGTAGSG 190 200 210 220 230 240 LGGIVFNLGM QSILQKRGVK WALIAQCIIC TSLSTIALML TRTTHQGLRQ HKRSYKFELL 250 260 270 280 290 300 DYDVLSNFAV WLLFGFVSFA MLGYVVLLYS LSDFTVSLGY TSKQGSYVSC MVSVGSLLGR 310 320 330 340 350 360 PIVGHIADKY GSLTVGMILH LVMAILCWAM WIPCKNLATA IRFGLLVGSI MGTIWPTIAS 370 380 390 400 410 420 IVTRIVGLQK LPGTFGSTWI FMAAFALVAP IIGLELRSTD TNGNDYYRTA IFVGFAYFGV 430 440 450 460 470 SLCQWLLRGF IIARDEIAVR EAYSADQNEL HLNVKLSHMS KCLFRYKQLP RRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 13.2 kb segment next to the left telomere of yeast chromosome XI revealed five open reading frames and recent recombination events with the right arms of chromosomes III and V." Alexandraki D., Tzermia M. Yeast 10:S81-S91(1994) [PubMed: 8091865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane." Makuc J., Paiva S., Schauen M., Kramer R., Andre B., Casal M., Leao C., Boles E. Yeast 18:1131-1143(2001) [PubMed: 11536335] [Abstract] Cited for: LACK OF FUNCTION AS A MONOCARBOXYLATE TRANSPORTER.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75950 Genomic DNA. Translation: CAA53552.1. Z28221 Genomic DNA. Translation: CAA82066.1.
PIR	S38065.
RefSeq	NP_012701.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4126N.
IntAct	P36032. 2 interactions.
Genome annotation databases
Ensembl	YKL221W; YKL221W; YKL221W; Saccharomyces cerevisiae. [Genome view]
GeneID	853659.
KEGG	sce:YKL221W.
NMPDR	fig|4932.3.peg.3679.
Organism-specific databases
CYGD	YKL221w.
SGD	S000001704. MCH2.
Phylogenomic databases
HOGENOM	P36032.
OrthoDB	EOG99KH6Q
Gene expression databases
ArrayExpress	P36032.
Genevestigator	P36032.
GermOnline	YKL221W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011701. MFS_1. IPR016196. MFS_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974581.

Entry information

Entry name

MCH2_YEAST

Accession

Primary (citable) accession number: P36032

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)