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P36032 (MCH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable transporter MCH2
Gene names
Name:MCH2
Ordered Locus Names:YKL221W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transporter. Does not act in the transport of monocarboxylic acids across the plasma membrane. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]

Ontologies

Keywords
   Biological processSymport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionsymporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Probable transporter MCH2
PRO_0000211403

Regions

Topological domain1 – 3737Cytoplasmic Potential
Transmembrane38 – 5821Helical; Potential
Topological domain59 – 8325Extracellular Potential
Transmembrane84 – 10522Helical; Potential
Topological domain106 – 1116Cytoplasmic Potential
Transmembrane112 – 13524Helical; Potential
Topological domain136 – 1416Extracellular Potential
Transmembrane142 – 16322Helical; Potential
Topological domain164 – 1696Cytoplasmic Potential
Transmembrane170 – 18617Helical; Potential
Topological domain187 – 20014Extracellular Potential
Transmembrane201 – 22020Helical; Potential
Topological domain221 – 24323Cytoplasmic Potential
Transmembrane244 – 26825Helical; Potential
Topological domain269 – 28618Extracellular Potential
Transmembrane287 – 30418Helical; Potential
Topological domain305 – 3128Cytoplasmic Potential
Transmembrane313 – 33220Helical; Potential
Topological domain333 – 34210Extracellular Potential
Transmembrane343 – 36220Helical; Potential
Topological domain363 – 3708Cytoplasmic Potential
Transmembrane371 – 39424Helical; Potential
Topological domain395 – 40814Extracellular Potential
Transmembrane409 – 43325Helical; Potential
Topological domain434 – 47340Cytoplasmic Potential

Amino acid modifications

Glycosylation721N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3421A → R in CAA53552. Ref.1
Sequence conflict3421A → R in CAA82066. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P36032 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 6C58D5DB3BAB5510

FASTA47352,257
        10         20         30         40         50         60 
MSEERHEDHH RDVENKLNLN GKDDINGNTS ISIEVPDGGY GWFILLAFIL YNFSTWGANS 

        70         80         90        100        110        120 
GYAIYLAHYL ENNTFAGGSK LDYASIGGLA FSCGLFFAPV ITWLYHIFSI QFIIGLGILF 

       130        140        150        160        170        180 
QGAALLLAAF SVTLWEIYLT QGVLIGFGLA FIFIPSVTLI PLWFRNKRSL ASGIGTAGSG 

       190        200        210        220        230        240 
LGGIVFNLGM QSILQKRGVK WALIAQCIIC TSLSTIALML TRTTHQGLRQ HKRSYKFELL 

       250        260        270        280        290        300 
DYDVLSNFAV WLLFGFVSFA MLGYVVLLYS LSDFTVSLGY TSKQGSYVSC MVSVGSLLGR 

       310        320        330        340        350        360 
PIVGHIADKY GSLTVGMILH LVMAILCWAM WIPCKNLATA IAFGLLVGSI MGTIWPTIAS 

       370        380        390        400        410        420 
IVTRIVGLQK LPGTFGSTWI FMAAFALVAP IIGLELRSTD TNGNDYYRTA IFVGFAYFGV 

       430        440        450        460        470 
SLCQWLLRGF IIARDEIAVR EAYSADQNEL HLNVKLSHMS KCLFRYKQLP RRV

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 13.2 kb segment next to the left telomere of yeast chromosome XI revealed five open reading frames and recent recombination events with the right arms of chromosomes III and V."
Alexandraki D., Tzermia M.
Yeast 10:S81-S91(1994) [PubMed: 8091865] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 342.
Strain: ATCC 204508 / S288c.
[4]"The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane."
Makuc J., Paiva S., Schauen M., Kramer R., Andre B., Casal M., Leao C., Boles E.
Yeast 18:1131-1143(2001) [PubMed: 11536335] [Abstract]
Cited for: LACK OF FUNCTION AS A MONOCARBOXYLATE TRANSPORTER.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X75950 Genomic DNA. Translation: CAA53552.1.
Z28221 Genomic DNA. Translation: CAA82066.1.
BK006944 Genomic DNA. Translation: DAA08948.2.
PIRS38065.
RefSeqNP_012701.2. NM_001179786.2.

3D structure databases

ProteinModelPortalP36032.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4126N.
MINTMINT-564557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID853659.
KEGGsce:YKL221W.
NMPDRfig|4932.3.peg.3679.

Organism-specific databases

CYGDYKL221w.
SGDS000001704. MCH2.

Phylogenomic databases

eggNOGfuNOG07803.
GeneTreeEFGT00050000000299.
HOGENOMHBG395884.
OMAHYLENNT.
OrthoDBEOG4N33XN.

Gene expression databases

ArrayExpressP36032.
GenevestigatorP36032.
GermOnlineYKL221W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
ProtoNetSearch...

Other

NextBio974581.

Entry information

Entry nameMCH2_YEAST
AccessionPrimary (citable) accession number: P36032
Secondary accession number(s): D6VWY2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: September 21, 2011
Last modified: December 14, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents