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Protein

Probable transporter MCH2

Gene

MCH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable transporter. Does not act in the transport of monocarboxylic acids across the plasma membrane.

GO - Molecular functioni

  • symporter activity Source: UniProtKB-KW
  • transporter activity Source: SGD

GO - Biological processi

  • transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31976-MONOMER.

Protein family/group databases

TCDBi2.A.1.13.18. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Probable transporter MCH2
Gene namesi
Name:MCH2
Ordered Locus Names:YKL221W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL221W.
SGDiS000001704. MCH2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737CytoplasmicSequence analysisAdd
BLAST
Transmembranei38 – 5821HelicalSequence analysisAdd
BLAST
Topological domaini59 – 8325ExtracellularSequence analysisAdd
BLAST
Transmembranei84 – 10522HelicalSequence analysisAdd
BLAST
Topological domaini106 – 1116CytoplasmicSequence analysis
Transmembranei112 – 13524HelicalSequence analysisAdd
BLAST
Topological domaini136 – 1416ExtracellularSequence analysis
Transmembranei142 – 16322HelicalSequence analysisAdd
BLAST
Topological domaini164 – 1696CytoplasmicSequence analysis
Transmembranei170 – 18617HelicalSequence analysisAdd
BLAST
Topological domaini187 – 20014ExtracellularSequence analysisAdd
BLAST
Transmembranei201 – 22020HelicalSequence analysisAdd
BLAST
Topological domaini221 – 24323CytoplasmicSequence analysisAdd
BLAST
Transmembranei244 – 26825HelicalSequence analysisAdd
BLAST
Topological domaini269 – 28618ExtracellularSequence analysisAdd
BLAST
Transmembranei287 – 30418HelicalSequence analysisAdd
BLAST
Topological domaini305 – 3128CytoplasmicSequence analysis
Transmembranei313 – 33220HelicalSequence analysisAdd
BLAST
Topological domaini333 – 34210ExtracellularSequence analysis
Transmembranei343 – 36220HelicalSequence analysisAdd
BLAST
Topological domaini363 – 3708CytoplasmicSequence analysis
Transmembranei371 – 39424HelicalSequence analysisAdd
BLAST
Topological domaini395 – 40814ExtracellularSequence analysisAdd
BLAST
Transmembranei409 – 43325HelicalSequence analysisAdd
BLAST
Topological domaini434 – 47340CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Probable transporter MCH2PRO_0000211403Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

BioGridi33944. 8 interactions.
DIPiDIP-4126N.
IntActiP36032. 1 interaction.
MINTiMINT-564557.

Structurei

3D structure databases

ProteinModelPortaliP36032.
SMRiP36032. Positions 166-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000112463.
HOGENOMiHOG000248493.
InParanoidiP36032.
OrthoDBiEOG75F4P0.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

P36032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEERHEDHH RDVENKLNLN GKDDINGNTS ISIEVPDGGY GWFILLAFIL
60 70 80 90 100
YNFSTWGANS GYAIYLAHYL ENNTFAGGSK LDYASIGGLA FSCGLFFAPV
110 120 130 140 150
ITWLYHIFSI QFIIGLGILF QGAALLLAAF SVTLWEIYLT QGVLIGFGLA
160 170 180 190 200
FIFIPSVTLI PLWFRNKRSL ASGIGTAGSG LGGIVFNLGM QSILQKRGVK
210 220 230 240 250
WALIAQCIIC TSLSTIALML TRTTHQGLRQ HKRSYKFELL DYDVLSNFAV
260 270 280 290 300
WLLFGFVSFA MLGYVVLLYS LSDFTVSLGY TSKQGSYVSC MVSVGSLLGR
310 320 330 340 350
PIVGHIADKY GSLTVGMILH LVMAILCWAM WIPCKNLATA IAFGLLVGSI
360 370 380 390 400
MGTIWPTIAS IVTRIVGLQK LPGTFGSTWI FMAAFALVAP IIGLELRSTD
410 420 430 440 450
TNGNDYYRTA IFVGFAYFGV SLCQWLLRGF IIARDEIAVR EAYSADQNEL
460 470
HLNVKLSHMS KCLFRYKQLP RRV
Length:473
Mass (Da):52,257
Last modified:September 21, 2011 - v2
Checksum:i6C58D5DB3BAB5510
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti342 – 3421A → R in CAA53552 (PubMed:8091865).Curated
Sequence conflicti342 – 3421A → R in CAA82066 (PubMed:8196765).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75950 Genomic DNA. Translation: CAA53552.1.
Z28221 Genomic DNA. Translation: CAA82066.1.
BK006944 Genomic DNA. Translation: DAA08948.2.
PIRiS38065.
RefSeqiNP_012701.2. NM_001179786.2.

Genome annotation databases

EnsemblFungiiYKL221W; YKL221W; YKL221W.
GeneIDi853659.
KEGGisce:YKL221W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75950 Genomic DNA. Translation: CAA53552.1.
Z28221 Genomic DNA. Translation: CAA82066.1.
BK006944 Genomic DNA. Translation: DAA08948.2.
PIRiS38065.
RefSeqiNP_012701.2. NM_001179786.2.

3D structure databases

ProteinModelPortaliP36032.
SMRiP36032. Positions 166-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33944. 8 interactions.
DIPiDIP-4126N.
IntActiP36032. 1 interaction.
MINTiMINT-564557.

Protein family/group databases

TCDBi2.A.1.13.18. the major facilitator superfamily (mfs).

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL221W; YKL221W; YKL221W.
GeneIDi853659.
KEGGisce:YKL221W.

Organism-specific databases

EuPathDBiFungiDB:YKL221W.
SGDiS000001704. MCH2.

Phylogenomic databases

GeneTreeiENSGT00730000112463.
HOGENOMiHOG000248493.
InParanoidiP36032.
OrthoDBiEOG75F4P0.

Enzyme and pathway databases

BioCyciYEAST:G3O-31976-MONOMER.

Miscellaneous databases

NextBioi974581.
PROiP36032.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of a 13.2 kb segment next to the left telomere of yeast chromosome XI revealed five open reading frames and recent recombination events with the right arms of chromosomes III and V."
    Alexandraki D., Tzermia M.
    Yeast 10:S81-S91(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 342.
    Strain: ATCC 204508 / S288c.
  4. "The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane."
    Makuc J., Paiva S., Schauen M., Kramer R., Andre B., Casal M., Leao C., Boles E.
    Yeast 18:1131-1143(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF FUNCTION AS A MONOCARBOXYLATE TRANSPORTER.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiMCH2_YEAST
AccessioniPrimary (citable) accession number: P36032
Secondary accession number(s): D6VWY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: September 21, 2011
Last modified: May 11, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.