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P36029 (TPO5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyamine transporter TPO5
Gene names
Name:TPO5
Ordered Locus Names:YKL174C
ORF Names:YKL639
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length618 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for polyamine transport. Transports putrescine effectively and spermidine less effectively. Ref.5

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.5.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 618618Polyamine transporter TPO5
PRO_0000054165

Regions

Topological domain1 – 6060Cytoplasmic Potential
Transmembrane61 – 8424Helical; Potential
Topological domain85 – 906Extracellular Potential
Transmembrane91 – 11020Helical; Potential
Topological domain111 – 13121Cytoplasmic Potential
Transmembrane132 – 14817Helical; Potential
Topological domain149 – 1546Extracellular Potential
Transmembrane155 – 17117Helical; Potential
Topological domain172 – 1798Cytoplasmic Potential
Transmembrane180 – 20021Helical; Potential
Topological domain201 – 21111Extracellular Potential
Transmembrane212 – 23120Helical; Potential
Topological domain232 – 29766Cytoplasmic Potential
Transmembrane298 – 31720Helical; Potential
Topological domain318 – 34225Extracellular Potential
Transmembrane343 – 36725Helical; Potential
Topological domain368 – 40235Cytoplasmic Potential
Transmembrane403 – 41917Helical; Potential
Topological domain420 – 4256Extracellular Potential
Transmembrane426 – 44924Helical; Potential
Topological domain450 – 46415Cytoplasmic Potential
Transmembrane465 – 48622Helical; Potential
Topological domain487 – 49812Extracellular Potential
Transmembrane499 – 51618Helical; Potential
Topological domain517 – 618102Cytoplasmic Potential

Amino acid modifications

Modified residue5371Phosphoserine Ref.7
Modified residue5691Phosphoserine Ref.4 Ref.7

Sequences

Sequence LengthMass (Da)Tools
P36029 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 035CB927C624CE74

FASTA61869,243
        10         20         30         40         50         60 
MPEYTLLADN IRENIVHFDP NGLFDNLHTI VHEDDSQENE EAEHFNYDQV LDKSLLSRGS 

        70         80         90        100        110        120 
IVGLGLGLMS PVLGMCTSMA IGLINGGPLT IMLGFLISGV CIWFSSLSLG EIVSKFPMEL 

       130        140        150        160        170        180 
HVGSAMLAPE KLKLVCSWYT GWLMLIGNWT MSTSITFAGA QLTISLILMT NSNLISEAHL 

       190        200        210        220        230        240 
IFYTVIVFYL VVTVVGLVNL KFARFIETIN KVCVYWIIYA IIFIDILLLV FHKGKFRSLK 

       250        260        270        280        290        300 
YALFHFDNNL SGYKSAFLSF IIGFQQSNFT LQGFSMLPAL ADEVKVPEKD IPRGMSNAVL 

       310        320        330        340        350        360 
LSAFSGVIFL IPIMLILPDN DLLFTNHKVL PIVNIFTKST DSVVLSFFLV LLILGNLLFS 

       370        380        390        400        410        420 
GIGSITTSSR AVYSFSRDQA IPYYDKWTYV EPDSQSKVPK NSVVLSMIIS YFLGLLALIS 

       430        440        450        460        470        480 
TAAFNAFIGA AVLCLCSATF IPLVLVLFTR RRAIRSAPVK IRYKFGWFIN IVSIVWLLLS 

       490        500        510        520        530        540 
MVSVCLPTQV PVTFKTMNYA LMVYVFCILV ITGLYFKWGK YNFRLPLADD IKAPIPSDAE 

       550        560        570        580        590        600 
ETVFELEDSN VEHTLNSGTT VKESVENNSE EGFIKVHPKS STENPFEENE ENVITDYGDE 

       610 
HHTAEQEFDL ADDRRYDI

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-569, MASS SPECTROMETRY.
Strain: SUB592.
[5]"Excretion of putrescine and spermidine by the protein encoded by YKL174c (TPO5) in Saccharomyces cerevisiae."
Tachihara K., Uemura T., Kashiwagi K., Igarashi K.
J. Biol. Chem. 280:12637-12642(2005) [PubMed: 15668236] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537 AND SER-569, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z26878 Genomic DNA. Translation: CAA81512.1.
Z28174 Genomic DNA. Translation: CAA82016.1.
BK006944 Genomic DNA. Translation: DAA08992.1.
PIRS38004.
RefSeqNP_012747.1. NM_001179740.1.

3D structure databases

ProteinModelPortalP36029.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4038N.
IntActP36029. 1 interaction.
MINTMINT-485381.
STRINGP36029.

Protein family/group databases

TCDB2.A.3.4.5. amino acid-polyamine-organocation (APC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL174C; YKL174C; YKL174C.
GeneID853680.
KEGGsce:YKL174C.
NMPDRfig|4932.3.peg.3725.

Organism-specific databases

CYGDYKL174c.
SGDS000001657. TPO5.

Phylogenomic databases

eggNOGfuNOG05702.
GeneTreeEFGT00050000001135.
HOGENOMHBG202405.
OMATGVATIC.
OrthoDBEOG466ZVH.

Gene expression databases

ArrayExpressP36029.
GenevestigatorP36029.
GermOnlineYKL174C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
PROSITEPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974640.

Entry information

Entry nameTPO5_YEAST
AccessionPrimary (citable) accession number: P36029
Secondary accession number(s): D6VX26
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents