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Protein

Polyamine transporter TPO5

Gene

TPO5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for polyamine transport. Transports putrescine effectively and spermidine less effectively.1 Publication

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: InterPro
  • polyamine transmembrane transporter activity Source: SGD

GO - Biological processi

  • polyamine transmembrane transport Source: GOC
  • polyamine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31941-MONOMER.

Protein family/group databases

TCDBi2.A.3.4.5. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter TPO5
Gene namesi
Name:TPO5
Ordered Locus Names:YKL174C
ORF Names:YKL639
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL174C.
SGDiS000001657. TPO5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060CytoplasmicSequence analysisAdd
BLAST
Transmembranei61 – 8424HelicalSequence analysisAdd
BLAST
Topological domaini85 – 906ExtracellularSequence analysis
Transmembranei91 – 11020HelicalSequence analysisAdd
BLAST
Topological domaini111 – 13121CytoplasmicSequence analysisAdd
BLAST
Transmembranei132 – 14817HelicalSequence analysisAdd
BLAST
Topological domaini149 – 1546ExtracellularSequence analysis
Transmembranei155 – 17117HelicalSequence analysisAdd
BLAST
Topological domaini172 – 1798CytoplasmicSequence analysis
Transmembranei180 – 20021HelicalSequence analysisAdd
BLAST
Topological domaini201 – 21111ExtracellularSequence analysisAdd
BLAST
Transmembranei212 – 23120HelicalSequence analysisAdd
BLAST
Topological domaini232 – 29766CytoplasmicSequence analysisAdd
BLAST
Transmembranei298 – 31720HelicalSequence analysisAdd
BLAST
Topological domaini318 – 34225ExtracellularSequence analysisAdd
BLAST
Transmembranei343 – 36725HelicalSequence analysisAdd
BLAST
Topological domaini368 – 40235CytoplasmicSequence analysisAdd
BLAST
Transmembranei403 – 41917HelicalSequence analysisAdd
BLAST
Topological domaini420 – 4256ExtracellularSequence analysis
Transmembranei426 – 44924HelicalSequence analysisAdd
BLAST
Topological domaini450 – 46415CytoplasmicSequence analysisAdd
BLAST
Transmembranei465 – 48622HelicalSequence analysisAdd
BLAST
Topological domaini487 – 49812ExtracellularSequence analysisAdd
BLAST
Transmembranei499 – 51618HelicalSequence analysisAdd
BLAST
Topological domaini517 – 618102CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Polyamine transporter TPO5PRO_0000054165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei569 – 5691PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36029.
TopDownProteomicsiP36029.

PTM databases

iPTMnetiP36029.

Interactioni

Protein-protein interaction databases

BioGridi33964. 24 interactions.
DIPiDIP-4038N.
IntActiP36029. 3 interactions.
MINTiMINT-485381.

Structurei

3D structure databases

ProteinModelPortaliP36029.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000067168.
HOGENOMiHOG000162166.
InParanoidiP36029.
OMAiSSRAVYS.
OrthoDBiEOG7P02T0.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36029-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEYTLLADN IRENIVHFDP NGLFDNLHTI VHEDDSQENE EAEHFNYDQV
60 70 80 90 100
LDKSLLSRGS IVGLGLGLMS PVLGMCTSMA IGLINGGPLT IMLGFLISGV
110 120 130 140 150
CIWFSSLSLG EIVSKFPMEL HVGSAMLAPE KLKLVCSWYT GWLMLIGNWT
160 170 180 190 200
MSTSITFAGA QLTISLILMT NSNLISEAHL IFYTVIVFYL VVTVVGLVNL
210 220 230 240 250
KFARFIETIN KVCVYWIIYA IIFIDILLLV FHKGKFRSLK YALFHFDNNL
260 270 280 290 300
SGYKSAFLSF IIGFQQSNFT LQGFSMLPAL ADEVKVPEKD IPRGMSNAVL
310 320 330 340 350
LSAFSGVIFL IPIMLILPDN DLLFTNHKVL PIVNIFTKST DSVVLSFFLV
360 370 380 390 400
LLILGNLLFS GIGSITTSSR AVYSFSRDQA IPYYDKWTYV EPDSQSKVPK
410 420 430 440 450
NSVVLSMIIS YFLGLLALIS TAAFNAFIGA AVLCLCSATF IPLVLVLFTR
460 470 480 490 500
RRAIRSAPVK IRYKFGWFIN IVSIVWLLLS MVSVCLPTQV PVTFKTMNYA
510 520 530 540 550
LMVYVFCILV ITGLYFKWGK YNFRLPLADD IKAPIPSDAE ETVFELEDSN
560 570 580 590 600
VEHTLNSGTT VKESVENNSE EGFIKVHPKS STENPFEENE ENVITDYGDE
610
HHTAEQEFDL ADDRRYDI
Length:618
Mass (Da):69,243
Last modified:June 1, 1994 - v1
Checksum:i035CB927C624CE74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81512.1.
Z28174 Genomic DNA. Translation: CAA82016.1.
BK006944 Genomic DNA. Translation: DAA08992.1.
PIRiS38004.
RefSeqiNP_012747.1. NM_001179740.1.

Genome annotation databases

EnsemblFungiiYKL174C; YKL174C; YKL174C.
GeneIDi853680.
KEGGisce:YKL174C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81512.1.
Z28174 Genomic DNA. Translation: CAA82016.1.
BK006944 Genomic DNA. Translation: DAA08992.1.
PIRiS38004.
RefSeqiNP_012747.1. NM_001179740.1.

3D structure databases

ProteinModelPortaliP36029.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33964. 24 interactions.
DIPiDIP-4038N.
IntActiP36029. 3 interactions.
MINTiMINT-485381.

Protein family/group databases

TCDBi2.A.3.4.5. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP36029.

Proteomic databases

MaxQBiP36029.
TopDownProteomicsiP36029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL174C; YKL174C; YKL174C.
GeneIDi853680.
KEGGisce:YKL174C.

Organism-specific databases

EuPathDBiFungiDB:YKL174C.
SGDiS000001657. TPO5.

Phylogenomic databases

GeneTreeiENSGT00530000067168.
HOGENOMiHOG000162166.
InParanoidiP36029.
OMAiSSRAVYS.
OrthoDBiEOG7P02T0.

Enzyme and pathway databases

BioCyciYEAST:G3O-31941-MONOMER.

Miscellaneous databases

PROiP36029.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Excretion of putrescine and spermidine by the protein encoded by YKL174c (TPO5) in Saccharomyces cerevisiae."
    Tachihara K., Uemura T., Kashiwagi K., Igarashi K.
    J. Biol. Chem. 280:12637-12642(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-569, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPO5_YEAST
AccessioniPrimary (citable) accession number: P36029
Secondary accession number(s): D6VX26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 8, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.