GTP-binding protein YPT52 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36018 (YPT52_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein YPT52

Gene names

Name:	YPT52
Ordered Locus Names:	YKR014C
ORF Names:	YK112

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for transport in the endocytic pathway and for correct sorting of the vacuolar hydrolases suggesting a possible intersection of the endocytic with the vacuolar sorting pathway. Ref.9
Subunit structure	Interacts with ROY1, YIF1, YIP3, YIP4 and YIP5. Ref.5 Ref.6 Ref.9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Endoplasmic reticulum. Note: Also found in association with endoplasmic reticulum vesicles having SED5 on their surface. Ref.9
Miscellaneous	Present with 9380 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Endoplasmic reticulum Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from mutant phenotype Ref.1. Source: SGD protein targeting to vacuole Inferred from mutant phenotype Ref.1. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from mutant phenotype. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.5 Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
GDI1	P39958	5	EBI-29407,EBI-7517
MRS6	P32864	3	EBI-29407,EBI-14799
ROY1	Q04847	3	EBI-29407,EBI-27556
YIP3	P53633	2	EBI-29407,EBI-25301
YIP4	P53093	2	EBI-29407,EBI-24124

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 234

234

GTP-binding protein YPT52

PRO_0000121326

Regions

Nucleotide binding

10 – 17

GTP By similarity

Nucleotide binding

66 – 70

GTP By similarity

Nucleotide binding

111 – 114

GTP By similarity

Amino acid modifications

Modified residue

Phosphothreonine Ref.8

Modified residue

135

Phosphoserine Ref.8

Lipidation

232

S-geranylgeranyl cysteine By similarity

Lipidation

233

S-geranylgeranyl cysteine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P36018 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 34AB2FFE38697261
Show »

FASTA

234

26,132

        10         20         30         40         50         60 
MLQFKLVLLG DSSVGKSSIV HRFVKDTFDE LRESTIGAAF LSQSITIHPN DGNETKDVVI 

        70         80         90        100        110        120 
KFEIWDTAGQ ERYKSLAPMY YRNANAALVV YDITQEDSLQ KARNWVDELK NKVGDDDLVI 

       130        140        150        160        170        180 
YLLGNKVDLC QETPSTETSP DSNEGGDEEQ KVRAISTEEA KQYAQEQGLL FREVSAKTGE 

       190        200        210        220        230 
GVKEIFQDIG EKLYDLKKDE ILSKQNRQIG GGNNGQVDIN LQRPSTNDPT SCCS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast." Singer-Krueger B., Stenmark H., Duesterhoeft A., Philippsen P., Yoo J.-S., Gallwitz D., Zerial M. J. Cell Biol. 125:283-298(1994) [PubMed: 8163546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p." Cho J.-H., Noda Y., Yoda K. FEBS Lett. 469:151-154(2000) [PubMed: 10713261] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-8, ASSOCIATION WITH SED5 VESICLES.
[5]	"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins." Calero M., Collins R.N. Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed: 11785952] [Abstract] Cited for: INTERACTION WITH YIP3.
[6]	"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors." Calero M., Winand N.J., Collins R.N. FEBS Lett. 515:89-98(2002) [PubMed: 11943201] [Abstract] Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-46 AND SER-135, MASS SPECTROMETRY.
[9]	"Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function." Liu Y., Nakatsukasa K., Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., Kamura T. Mol. Biol. Cell 22:1575-1584(2011) [PubMed: 21389113] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ROY1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76174 Genomic DNA. Translation: CAA53770.1. Z28239 Genomic DNA. Translation: CAA82086.1. BK006944 Genomic DNA. Translation: DAA09169.1.
PIR	S38083.
RefSeq	NP_012939.1. NM_001179804.1.
3D structure databases
ProteinModelPortal	P36018.
SMR	P36018. Positions 1-233.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2121N.
IntAct	P36018. 15 interactions.
MINT	MINT-541697.
STRING	P36018.
Proteomic databases
PeptideAtlas	P36018.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR014C; YKR014C; YKR014C.
GeneID	853884.
KEGG	sce:YKR014C.
NMPDR	fig\|4932.3.peg.3925.
Organism-specific databases
CYGD	YKR014c.
SGD	S000001722. YPT52.
Phylogenomic databases
eggNOG	fuNOG07709.
GeneTree	EFGT00050000002458.
HOGENOM	HBG745225.
OMA	VHRFVKD.
OrthoDB	EOG4S1XHJ.
Gene expression databases
ArrayExpress	P36018.
Genevestigator	P36018.
GermOnline	YKR014C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
KO	K07976.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975177.

Entry information

Entry name

YPT52_YEAST

Accession

Primary (citable) accession number: P36018
Secondary accession number(s): D6VX79

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents