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P36018 (YPT52_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein YPT52
Gene names
Name:YPT52
Ordered Locus Names:YKR014C
ORF Names:YK112
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length234 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for transport in the endocytic pathway and for correct sorting of the vacuolar hydrolases suggesting a possible intersection of the endocytic with the vacuolar sorting pathway. Ref.9

Subunit structure

Interacts with ROY1, YIF1, YIP3, YIP4 and YIP5. Ref.5 Ref.6 Ref.9

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Endoplasmic reticulum. Note: Also found in association with endoplasmic reticulum vesicles having SED5 on their surface. Ref.9

Miscellaneous

Present with 9380 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell membrane
Endoplasmic reticulum
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Phosphoprotein
Prenylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay PubMed 11210571. Source: GOC

Golgi to endosome transport

Inferred from mutant phenotype PubMed 22748138. Source: SGD

endocytosis

Inferred from mutant phenotype Ref.1. Source: SGD

late endosome to vacuole transport via multivesicular body sorting pathway

Inferred from genetic interaction PubMed 22748138. Source: SGD

multivesicular body assembly

Inferred from genetic interaction PubMed 22748138. Source: SGD

protein localization to endosome

Inferred from genetic interaction PubMed 23264632. Source: SGD

protein targeting to vacuole

Inferred from mutant phenotype Ref.1. Source: SGD

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentendoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

late endosome

Inferred from mutant phenotype PubMed 11872141. Source: SGD

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from direct assay PubMed 11210571. Source: SGD

protein binding

Inferred from physical interaction Ref.5PubMed 11805826Ref.6PubMed 16429126PubMed 18467557PubMed 18719252. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 234234GTP-binding protein YPT52
PRO_0000121326

Regions

Nucleotide binding10 – 178GTP By similarity
Nucleotide binding66 – 705GTP By similarity
Nucleotide binding111 – 1144GTP By similarity

Amino acid modifications

Modified residue1391Phosphoserine Ref.8
Modified residue1421Phosphoserine Ref.8
Lipidation2321S-geranylgeranyl cysteine By similarity
Lipidation2331S-geranylgeranyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P36018 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 34AB2FFE38697261

FASTA23426,132
        10         20         30         40         50         60 
MLQFKLVLLG DSSVGKSSIV HRFVKDTFDE LRESTIGAAF LSQSITIHPN DGNETKDVVI 

        70         80         90        100        110        120 
KFEIWDTAGQ ERYKSLAPMY YRNANAALVV YDITQEDSLQ KARNWVDELK NKVGDDDLVI 

       130        140        150        160        170        180 
YLLGNKVDLC QETPSTETSP DSNEGGDEEQ KVRAISTEEA KQYAQEQGLL FREVSAKTGE 

       190        200        210        220        230 
GVKEIFQDIG EKLYDLKKDE ILSKQNRQIG GGNNGQVDIN LQRPSTNDPT SCCS 

« Hide

References

« Hide 'large scale' references
[1]"Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast."
Singer-Krueger B., Stenmark H., Duesterhoeft A., Philippsen P., Yoo J.-S., Gallwitz D., Zerial M.
J. Cell Biol. 125:283-298(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
Cho J.-H., Noda Y., Yoda K.
FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-8, ASSOCIATION WITH SED5 VESICLES.
[5]"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
Calero M., Collins R.N.
Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP3.
[6]"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
Calero M., Winand N.J., Collins R.N.
FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND SER-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function."
Liu Y., Nakatsukasa K., Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., Kamura T.
Mol. Biol. Cell 22:1575-1584(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ROY1.
[10]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76174 Genomic DNA. Translation: CAA53770.1.
Z28239 Genomic DNA. Translation: CAA82086.1.
BK006944 Genomic DNA. Translation: DAA09169.1.
PIRS38083.
RefSeqNP_012939.1. NM_001179804.1.

3D structure databases

ProteinModelPortalP36018.
SMRP36018. Positions 5-193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34146. 46 interactions.
DIPDIP-2121N.
IntActP36018. 13 interactions.
MINTMINT-541697.
STRING4932.YKR014C.

Proteomic databases

MaxQBP36018.
PaxDbP36018.
PeptideAtlasP36018.
PRIDEP36018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR014C; YKR014C; YKR014C.
GeneID853884.
KEGGsce:YKR014C.

Organism-specific databases

CYGDYKR014c.
SGDS000001722. YPT52.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00740000114962.
HOGENOMHOG000233968.
KOK07889.
OMAKRAVDHQ.
OrthoDBEOG7BGHXR.

Enzyme and pathway databases

BioCycYEAST:G3O-31990-MONOMER.

Gene expression databases

GenevestigatorP36018.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975177.

Entry information

Entry nameYPT52_YEAST
AccessionPrimary (citable) accession number: P36018
Secondary accession number(s): D6VX79
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families