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P36018

- YPT52_YEAST

UniProt

P36018 - YPT52_YEAST

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Protein
GTP-binding protein YPT52
Gene
YPT52, YKR014C, YK112
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for transport in the endocytic pathway and for correct sorting of the vacuolar hydrolases suggesting a possible intersection of the endocytic with the vacuolar sorting pathway.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTP By similarity
Nucleotide bindingi66 – 705GTP By similarity
Nucleotide bindingi111 – 1144GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. Golgi to endosome transport Source: SGD
  3. endocytosis Source: SGD
  4. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  5. multivesicular body assembly Source: SGD
  6. protein localization to endosome Source: SGD
  7. protein targeting to vacuole Source: SGD
  8. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31990-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT52
Gene namesi
Name:YPT52
Ordered Locus Names:YKR014C
ORF Names:YK112
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR014c.
SGDiS000001722. YPT52.

Subcellular locationi

Cell membrane; Lipid-anchor; Cytoplasmic side Reviewed prediction. Endoplasmic reticulum
Note: Also found in association with endoplasmic reticulum vesicles having SED5 on their surface.1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
  2. late endosome Source: SGD
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 234234GTP-binding protein YPT52
PRO_0000121326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391Phosphoserine1 Publication
Modified residuei142 – 1421Phosphoserine1 Publication
Lipidationi232 – 2321S-geranylgeranyl cysteine By similarity
Lipidationi233 – 2331S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP36018.
PaxDbiP36018.
PeptideAtlasiP36018.
PRIDEiP36018.

Expressioni

Gene expression databases

GenevestigatoriP36018.

Interactioni

Subunit structurei

Interacts with ROY1, YIF1, YIP3, YIP4 and YIP5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GDI1P399583EBI-29407,EBI-7517
MRS6P328643EBI-29407,EBI-14799
ROY1Q048473EBI-29407,EBI-27556
YIP3P536332EBI-29407,EBI-25301
YIP4P530932EBI-29407,EBI-24124

Protein-protein interaction databases

BioGridi34146. 46 interactions.
DIPiDIP-2121N.
IntActiP36018. 13 interactions.
MINTiMINT-541697.
STRINGi4932.YKR014C.

Structurei

3D structure databases

ProteinModelPortaliP36018.
SMRiP36018. Positions 5-193.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00740000114962.
HOGENOMiHOG000233968.
KOiK07889.
OMAiKRAVDHQ.
OrthoDBiEOG7BGHXR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36018-1 [UniParc]FASTAAdd to Basket

« Hide

MLQFKLVLLG DSSVGKSSIV HRFVKDTFDE LRESTIGAAF LSQSITIHPN    50
DGNETKDVVI KFEIWDTAGQ ERYKSLAPMY YRNANAALVV YDITQEDSLQ 100
KARNWVDELK NKVGDDDLVI YLLGNKVDLC QETPSTETSP DSNEGGDEEQ 150
KVRAISTEEA KQYAQEQGLL FREVSAKTGE GVKEIFQDIG EKLYDLKKDE 200
ILSKQNRQIG GGNNGQVDIN LQRPSTNDPT SCCS 234
Length:234
Mass (Da):26,132
Last modified:June 1, 1994 - v1
Checksum:i34AB2FFE38697261
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76174 Genomic DNA. Translation: CAA53770.1.
Z28239 Genomic DNA. Translation: CAA82086.1.
BK006944 Genomic DNA. Translation: DAA09169.1.
PIRiS38083.
RefSeqiNP_012939.1. NM_001179804.1.

Genome annotation databases

EnsemblFungiiYKR014C; YKR014C; YKR014C.
GeneIDi853884.
KEGGisce:YKR014C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76174 Genomic DNA. Translation: CAA53770.1 .
Z28239 Genomic DNA. Translation: CAA82086.1 .
BK006944 Genomic DNA. Translation: DAA09169.1 .
PIRi S38083.
RefSeqi NP_012939.1. NM_001179804.1.

3D structure databases

ProteinModelPortali P36018.
SMRi P36018. Positions 5-193.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34146. 46 interactions.
DIPi DIP-2121N.
IntActi P36018. 13 interactions.
MINTi MINT-541697.
STRINGi 4932.YKR014C.

Proteomic databases

MaxQBi P36018.
PaxDbi P36018.
PeptideAtlasi P36018.
PRIDEi P36018.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR014C ; YKR014C ; YKR014C .
GeneIDi 853884.
KEGGi sce:YKR014C.

Organism-specific databases

CYGDi YKR014c.
SGDi S000001722. YPT52.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00740000114962.
HOGENOMi HOG000233968.
KOi K07889.
OMAi KRAVDHQ.
OrthoDBi EOG7BGHXR.

Enzyme and pathway databases

BioCyci YEAST:G3O-31990-MONOMER.

Miscellaneous databases

NextBioi 975177.

Gene expression databases

Genevestigatori P36018.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast."
    Singer-Krueger B., Stenmark H., Duesterhoeft A., Philippsen P., Yoo J.-S., Gallwitz D., Zerial M.
    J. Cell Biol. 125:283-298(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
    Cho J.-H., Noda Y., Yoda K.
    FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-8, ASSOCIATION WITH SED5 VESICLES.
  5. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
    Calero M., Collins R.N.
    Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIP3.
  6. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
    Calero M., Winand N.J., Collins R.N.
    FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND SER-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function."
    Liu Y., Nakatsukasa K., Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., Kamura T.
    Mol. Biol. Cell 22:1575-1584(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ROY1.
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYPT52_YEAST
AccessioniPrimary (citable) accession number: P36018
Secondary accession number(s): D6VX79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9380 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi