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P36018

- YPT52_YEAST

UniProt

P36018 - YPT52_YEAST

Protein

GTP-binding protein YPT52

Gene

YPT52

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Required for transport in the endocytic pathway and for correct sorting of the vacuolar hydrolases suggesting a possible intersection of the endocytic with the vacuolar sorting pathway.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTPBy similarity
    Nucleotide bindingi66 – 705GTPBy similarity
    Nucleotide bindingi111 – 1144GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. endocytosis Source: SGD
    2. Golgi to endosome transport Source: SGD
    3. GTP catabolic process Source: GOC
    4. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
    5. multivesicular body assembly Source: SGD
    6. protein localization to endosome Source: SGD
    7. protein targeting to vacuole Source: SGD
    8. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31990-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein YPT52
    Gene namesi
    Name:YPT52
    Ordered Locus Names:YKR014C
    ORF Names:YK112
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR014c.
    SGDiS000001722. YPT52.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Endoplasmic reticulum 1 Publication
    Note: Also found in association with endoplasmic reticulum vesicles having SED5 on their surface.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. late endosome Source: SGD
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 234234GTP-binding protein YPT52PRO_0000121326Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei139 – 1391Phosphoserine1 Publication
    Modified residuei142 – 1421Phosphoserine1 Publication
    Lipidationi232 – 2321S-geranylgeranyl cysteineBy similarity
    Lipidationi233 – 2331S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiP36018.
    PaxDbiP36018.
    PeptideAtlasiP36018.
    PRIDEiP36018.

    Expressioni

    Gene expression databases

    GenevestigatoriP36018.

    Interactioni

    Subunit structurei

    Interacts with ROY1, YIF1, YIP3, YIP4 and YIP5.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GDI1P399583EBI-29407,EBI-7517
    MRS6P328643EBI-29407,EBI-14799
    ROY1Q048473EBI-29407,EBI-27556
    YIP3P536332EBI-29407,EBI-25301
    YIP4P530932EBI-29407,EBI-24124

    Protein-protein interaction databases

    BioGridi34146. 46 interactions.
    DIPiDIP-2121N.
    IntActiP36018. 13 interactions.
    MINTiMINT-541697.
    STRINGi4932.YKR014C.

    Structurei

    3D structure databases

    ProteinModelPortaliP36018.
    SMRiP36018. Positions 5-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00740000114962.
    HOGENOMiHOG000233968.
    KOiK07889.
    OMAiKRAVDHQ.
    OrthoDBiEOG7BGHXR.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36018-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQFKLVLLG DSSVGKSSIV HRFVKDTFDE LRESTIGAAF LSQSITIHPN    50
    DGNETKDVVI KFEIWDTAGQ ERYKSLAPMY YRNANAALVV YDITQEDSLQ 100
    KARNWVDELK NKVGDDDLVI YLLGNKVDLC QETPSTETSP DSNEGGDEEQ 150
    KVRAISTEEA KQYAQEQGLL FREVSAKTGE GVKEIFQDIG EKLYDLKKDE 200
    ILSKQNRQIG GGNNGQVDIN LQRPSTNDPT SCCS 234
    Length:234
    Mass (Da):26,132
    Last modified:June 1, 1994 - v1
    Checksum:i34AB2FFE38697261
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76174 Genomic DNA. Translation: CAA53770.1.
    Z28239 Genomic DNA. Translation: CAA82086.1.
    BK006944 Genomic DNA. Translation: DAA09169.1.
    PIRiS38083.
    RefSeqiNP_012939.1. NM_001179804.1.

    Genome annotation databases

    EnsemblFungiiYKR014C; YKR014C; YKR014C.
    GeneIDi853884.
    KEGGisce:YKR014C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76174 Genomic DNA. Translation: CAA53770.1 .
    Z28239 Genomic DNA. Translation: CAA82086.1 .
    BK006944 Genomic DNA. Translation: DAA09169.1 .
    PIRi S38083.
    RefSeqi NP_012939.1. NM_001179804.1.

    3D structure databases

    ProteinModelPortali P36018.
    SMRi P36018. Positions 5-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34146. 46 interactions.
    DIPi DIP-2121N.
    IntActi P36018. 13 interactions.
    MINTi MINT-541697.
    STRINGi 4932.YKR014C.

    Proteomic databases

    MaxQBi P36018.
    PaxDbi P36018.
    PeptideAtlasi P36018.
    PRIDEi P36018.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR014C ; YKR014C ; YKR014C .
    GeneIDi 853884.
    KEGGi sce:YKR014C.

    Organism-specific databases

    CYGDi YKR014c.
    SGDi S000001722. YPT52.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00740000114962.
    HOGENOMi HOG000233968.
    KOi K07889.
    OMAi KRAVDHQ.
    OrthoDBi EOG7BGHXR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31990-MONOMER.

    Miscellaneous databases

    NextBioi 975177.

    Gene expression databases

    Genevestigatori P36018.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast."
      Singer-Krueger B., Stenmark H., Duesterhoeft A., Philippsen P., Yoo J.-S., Gallwitz D., Zerial M.
      J. Cell Biol. 125:283-298(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
      Cho J.-H., Noda Y., Yoda K.
      FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-8, ASSOCIATION WITH SED5 VESICLES.
    5. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
      Calero M., Collins R.N.
      Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP3.
    6. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
      Calero M., Winand N.J., Collins R.N.
      FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139 AND SER-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function."
      Liu Y., Nakatsukasa K., Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., Kamura T.
      Mol. Biol. Cell 22:1575-1584(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ROY1.
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYPT52_YEAST
    AccessioniPrimary (citable) accession number: P36018
    Secondary accession number(s): D6VX79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 9380 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3